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The map label for this gene is tuf

Identifier: 150008881

GI number: 150008881

Start: 2666004

End: 2667191

Strand: Reverse

Name: tuf

Synonym: BDI_2272

Alternate gene names: 150008881

Gene position: 2667191-2666004 (Counterclockwise)

Preceding gene: 150008882

Following gene: 150008880

Centisome position: 55.44

GC content: 46.3

Gene sequence:

>1188_bases
ATGGCAAAAGAGAAATTTGACAGATCGAAACCACATGTGAACATTGGTACGATTGGTCACGTTGACCACGGTAAAACAAC
TTTGACAGCTGCTATCACAACAGTATTGGCAAAGAAAGGTCTTTCAGAGTTGCGTTCATTCGATTCTATCGACAACGCTC
CTGAAGAAAAAGAGAGAGGTATCACAATCAACACTTCTCACGTAGAGTATCAGACTGCAAACCGTCACTATGCTCACGTA
GACTGTCCGGGTCACGCCGACTACGTAAAGAACATGGTAACTGGTGCTGCTCAGATGGACGGTGCTATCATCGTAGTTGC
TGCTACTGATGGTCCTATGCCTCAAACTCGCGAGCACATCCTTTTGGCTCGTCAGGTAAACGTTCCGAGATTGGTTGTAT
TCATGAACAAGTGTGACATGGTTGACGACGAGGAAATGTTGGAATTGGTTGAGATGGAGATGAGAGAGTTGCTTTCATTC
TATCAATTCGACGGTGACAACACTCCGATCATCCGTGGTTCTGCTCTTGGTGCATTGAACGGTGATGCTCAATGGGAAGA
TAAAGTAATGGAGTTGATGGAAGCTTGTGATACTTGGATTCCTCTGCCTCCGCGCGAAATCGACAAGCCGTTCTTGATGC
CGGTTGAGGACGTATTCTCAATCACGGGTCGTGGTACTGTTGCTACAGGTCGTATCGAGACAGGTATTGTTAAGGTTGGT
GAGGAAGTTCAGATCATCGGTCTTGGCGCTGCTGGTAAGAAATCTGTTGTTACAGGTGTTGAGATGTTCCGTAAGTTATT
GGATCAAGGTGAGGCTGGTGATAACGTTGGTTTGTTGCTTCGCGGTATCGATAAGAATGAGATCAAGCGTGGTATGGTAA
TCTGCCACCCGGGTCAGGTTAAAGAGCATTCTAAGTTCAAGGCTGAGGTTTATATCTTGAAGAAAGAGGAAGGTGGTCGT
CACACTCCGTTCCACAACAAATATCGTCCTCAGTTCTATATCCGTACATTGGATGTAACTGGTGAGATCACTTTGCCGGA
AGGAACTGAAATGGTAATGCCGGGTGATAACGTAACGATCGAGGTTGAGTTGATCTATCCGGTAGCATGTAGCGTAGGTC
TTCGTTTCGCTATCCGCGAGGGTGGACGTACAGTAGGTGCAGGCCAGATCACAGAATTAGAGAACTAA

Upstream 100 bases:

>100_bases
AGCACTTCCTTGGTAAGGAAGAGGTCCCGAGTTCAAGTCTCGGCACCGGCTCAATTGAAAATTGAACATGATCATTAATC
AAATAAAGAACAATTAAGTT

Downstream 100 bases:

>100_bases
TTCAAGCATTAGATAATGTTATAGCCAGTCTTTTTAAGGGCTGGCTATTTCTACGGAAGTAGCTCAGTTGGTAGAGCACT
GGTCTCCAAAACCAGGTGTC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGLSELRSFDSIDNAPEEKERGITINTSHVEYQTANRHYAHV
DCPGHADYVKNMVTGAAQMDGAIIVVAATDGPMPQTREHILLARQVNVPRLVVFMNKCDMVDDEEMLELVEMEMRELLSF
YQFDGDNTPIIRGSALGALNGDAQWEDKVMELMEACDTWIPLPPREIDKPFLMPVEDVFSITGRGTVATGRIETGIVKVG
EEVQIIGLGAAGKKSVVTGVEMFRKLLDQGEAGDNVGLLLRGIDKNEIKRGMVICHPGQVKEHSKFKAEVYILKKEEGGR
HTPFHNKYRPQFYIRTLDVTGEITLPEGTEMVMPGDNVTIEVELIYPVACSVGLRFAIREGGRTVGAGQITELEN

Sequences:

>Translated_395_residues
MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGLSELRSFDSIDNAPEEKERGITINTSHVEYQTANRHYAHV
DCPGHADYVKNMVTGAAQMDGAIIVVAATDGPMPQTREHILLARQVNVPRLVVFMNKCDMVDDEEMLELVEMEMRELLSF
YQFDGDNTPIIRGSALGALNGDAQWEDKVMELMEACDTWIPLPPREIDKPFLMPVEDVFSITGRGTVATGRIETGIVKVG
EEVQIIGLGAAGKKSVVTGVEMFRKLLDQGEAGDNVGLLLRGIDKNEIKRGMVICHPGQVKEHSKFKAEVYILKKEEGGR
HTPFHNKYRPQFYIRTLDVTGEITLPEGTEMVMPGDNVTIEVELIYPVACSVGLRFAIREGGRTVGAGQITELEN
>Mature_394_residues
AKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGLSELRSFDSIDNAPEEKERGITINTSHVEYQTANRHYAHVD
CPGHADYVKNMVTGAAQMDGAIIVVAATDGPMPQTREHILLARQVNVPRLVVFMNKCDMVDDEEMLELVEMEMRELLSFY
QFDGDNTPIIRGSALGALNGDAQWEDKVMELMEACDTWIPLPPREIDKPFLMPVEDVFSITGRGTVATGRIETGIVKVGE
EVQIIGLGAAGKKSVVTGVEMFRKLLDQGEAGDNVGLLLRGIDKNEIKRGMVICHPGQVKEHSKFKAEVYILKKEEGGRH
TPFHNKYRPQFYIRTLDVTGEITLPEGTEMVMPGDNVTIEVELIYPVACSVGLRFAIREGGRTVGAGQITELEN

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=392, Percent_Identity=55.1020408163265, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI223555963, Length=437, Percent_Identity=30.2059496567506, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI5729864, Length=437, Percent_Identity=30.2059496567506, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4503475, Length=450, Percent_Identity=28.6666666666667, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI4503471, Length=451, Percent_Identity=28.159645232816, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI54607086, Length=316, Percent_Identity=29.746835443038, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI194018522, Length=344, Percent_Identity=27.0348837209302, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI194097354, Length=344, Percent_Identity=27.0348837209302, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI194018520, Length=344, Percent_Identity=27.0348837209302, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI46094014, Length=438, Percent_Identity=25.3424657534247, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI4503507, Length=267, Percent_Identity=28.4644194756554, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=36.8421052631579, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI310132016, Length=112, Percent_Identity=37.5, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI310110807, Length=112, Percent_Identity=37.5, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI310123363, Length=112, Percent_Identity=37.5, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI53729339, Length=230, Percent_Identity=30.4347826086957, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI53729337, Length=230, Percent_Identity=30.4347826086957, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=73.027989821883, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=73.027989821883, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI2367247, Length=281, Percent_Identity=33.4519572953737, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI48994988, Length=287, Percent_Identity=32.0557491289199, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789108, Length=310, Percent_Identity=30.3225806451613, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788922, Length=342, Percent_Identity=25.1461988304094, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=398, Percent_Identity=50.7537688442211, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25141371, Length=388, Percent_Identity=44.3298969072165, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI17552884, Length=448, Percent_Identity=28.7946428571429, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=448, Percent_Identity=28.7946428571429, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI32566303, Length=430, Percent_Identity=27.6744186046512, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=433, Percent_Identity=27.2517321016166, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=433, Percent_Identity=27.2517321016166, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=301, Percent_Identity=28.9036544850498, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=341, Percent_Identity=27.5659824046921, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17509919, Length=280, Percent_Identity=28.2142857142857, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32566301, Length=158, Percent_Identity=34.8101265822785, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=236, Percent_Identity=29.2372881355932, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=394, Percent_Identity=64.9746192893401, Blast_Score=523, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6325337, Length=459, Percent_Identity=29.6296296296296, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=459, Percent_Identity=29.6296296296296, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=455, Percent_Identity=24.3956043956044, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=281, Percent_Identity=28.8256227758007, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=342, Percent_Identity=27.1929824561404, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323098, Length=135, Percent_Identity=34.8148148148148, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=310, Percent_Identity=27.741935483871, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=391, Percent_Identity=55.7544757033248, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI17864358, Length=391, Percent_Identity=55.7544757033248, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI19921738, Length=387, Percent_Identity=49.6124031007752, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24652838, Length=447, Percent_Identity=27.9642058165548, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI17137572, Length=447, Percent_Identity=27.9642058165548, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI45553807, Length=334, Percent_Identity=31.1377245508982, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI45553816, Length=334, Percent_Identity=31.1377245508982, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24651721, Length=334, Percent_Identity=31.1377245508982, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17864154, Length=334, Percent_Identity=31.1377245508982, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=31.6831683168317, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI45550900, Length=343, Percent_Identity=28.2798833819242, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI17137380, Length=344, Percent_Identity=27.3255813953488, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=29.368029739777, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=29.368029739777, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28574573, Length=164, Percent_Identity=31.7073170731707, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582462, Length=159, Percent_Identity=30.8176100628931, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI28572034, Length=242, Percent_Identity=32.2314049586777, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_PARD8 (A6LE88)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303624.1
- ProteinModelPortal:   A6LE88
- SMR:   A6LE88
- STRING:   A6LE88
- GeneID:   5307421
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2272
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   RDKPFLM
- ProtClustDB:   PRK12735
- BioCyc:   PDIS435591:BDI_2272-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43611; Mature: 43479

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGLSELRSFDSIDNAPEEKERG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
ITINTSHVEYQTANRHYAHVDCPGHADYVKNMVTGAAQMDGAIIVVAATDGPMPQTREHI
EEEECCEEEEEECCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLARQVNVPRLVVFMNKCDMVDDEEMLELVEMEMRELLSFYQFDGDNTPIIRGSALGALN
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCC
GDAQWEDKVMELMEACDTWIPLPPREIDKPFLMPVEDVFSITGRGTVATGRIETGIVKVG
CCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHCCCCCEEEECCEEECEEECC
EEVQIIGLGAAGKKSVVTGVEMFRKLLDQGEAGDNVGLLLRGIDKNEIKRGMVICHPGQV
CEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCEEEECCCCC
KEHSKFKAEVYILKKEEGGRHTPFHNKYRPQFYIRTLDVTGEITLPEGTEMVMPGDNVTI
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEEECEEEEECCCCCEEEECCCCEEE
EVELIYPVACSVGLRFAIREGGRTVGAGQITELEN
EEEEEEEEECCCCEEEEEECCCCEECCCCEEECCC
>Mature Secondary Structure 
AKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGLSELRSFDSIDNAPEEKERG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
ITINTSHVEYQTANRHYAHVDCPGHADYVKNMVTGAAQMDGAIIVVAATDGPMPQTREHI
EEEECCEEEEEECCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLARQVNVPRLVVFMNKCDMVDDEEMLELVEMEMRELLSFYQFDGDNTPIIRGSALGALN
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCC
GDAQWEDKVMELMEACDTWIPLPPREIDKPFLMPVEDVFSITGRGTVATGRIETGIVKVG
CCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHCCCCCEEEECCEEECEEECC
EEVQIIGLGAAGKKSVVTGVEMFRKLLDQGEAGDNVGLLLRGIDKNEIKRGMVICHPGQV
CEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCEEEECCCCC
KEHSKFKAEVYILKKEEGGRHTPFHNKYRPQFYIRTLDVTGEITLPEGTEMVMPGDNVTI
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEEECEEEEECCCCCEEEECCCCEEE
EVELIYPVACSVGLRFAIREGGRTVGAGQITELEN
EEEEEEEEECCCCEEEEEECCCCEECCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA