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The map label for this gene is fcl [H]

Identifier: 150008812

GI number: 150008812

Start: 2591570

End: 2592511

Strand: Reverse

Name: fcl [H]

Synonym: BDI_2202

Alternate gene names: 150008812

Gene position: 2592511-2591570 (Counterclockwise)

Preceding gene: 150008813

Following gene: 150008811

Centisome position: 53.88

GC content: 52.44

Gene sequence:

>942_bases
ATGGAGAAGAACGCAAAAATCTATGTAGCAGGACACCGTGGAATGGTAGGCTCTGCCATTGTACGGGAACTGCAACGCCA
GGGATATACGAATATCATCACACGCACGCACAAAGAATTGGACTTGACTCGCCAGGAAGCCGTGGAACGCTTCTTCGCCG
AAGAAAAGCCCGAATATGTATTCCTCGCCGCAGCAAAGGTGGGCGGTATCATCGCCAACCAATCGGCATTGGCGGACTTC
ATGTACGAGAACATGATACTGGAGATGAATGTCATCCACTCGGCATGGCGCAATGGTTGCAGGAAGCTGGAGTTTCTCGG
TTCATCGTGCATATACCCGCGTATGGCACCCCAGCCGATGCCTGAAAGCTGCCTGCTAACCTCCGAACTGGAAAAGACCA
ACGAGGCATACGCATTGGCAAAAATCTCTGGATTGAAATACTGCGAGTTTCTCAACCGCCAGTACGGCACGGACTATATC
AGCGTGATGCCCACCAATCTCTATGGGCCGAATGACAACTACCACCCCGAGCACAGCCACGTGTTACCGGCGCTTATCCG
CCGCTTTCATGAGGCGAAGGAAGCGGGCTTGAAAGAGGTGACCTGCTGGGGTGACGGTAGCCCGTTGCGAGAGTTTCTCT
ATGTGGATGATTTGGCCAATCTCTGCGTATTCTTGATGAACAACTATTCGGGGTACGAGACAGTAAACGCCGGAACAGGA
AAGGAACTGACCATAAAGGCACTGACAGAATTAGTGGCAAAGATAATCGGCTACACCGGCGAGATACGCTGGGACACCTC
ACGGCCGAACGGTACCCCCCGCAAGCTGCTTGACGTTTCGAAAGCGACTTCGCTCGGATGGACATACAAGACGGAACTGG
AGGAAGGCATCCGTCTTTCCTACGAGGATTTCTTAAACAACCCGATGAGGGCGGAAAGATAA

Upstream 100 bases:

>100_bases
CCGATCTGGCAAAAGTGGCCGTGGAGCGTGCATCGCAACAGGTAAGAACCAACCTTGCCGAATATCTCGAAAAAGGAATC
GTAAAATAATCGAGAAAACA

Downstream 100 bases:

>100_bases
CTTTCACAACATAATCATGCAAATCATACTCTTATCCGGCGGTTCAGGTAAAAGGCTGTGGCCGCTTTCAAACGAATCCC
GCTCCAAACAATTTCTCAGA

Product: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MEKNAKIYVAGHRGMVGSAIVRELQRQGYTNIITRTHKELDLTRQEAVERFFAEEKPEYVFLAAAKVGGIIANQSALADF
MYENMILEMNVIHSAWRNGCRKLEFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNRQYGTDYI
SVMPTNLYGPNDNYHPEHSHVLPALIRRFHEAKEAGLKEVTCWGDGSPLREFLYVDDLANLCVFLMNNYSGYETVNAGTG
KELTIKALTELVAKIIGYTGEIRWDTSRPNGTPRKLLDVSKATSLGWTYKTELEEGIRLSYEDFLNNPMRAER

Sequences:

>Translated_313_residues
MEKNAKIYVAGHRGMVGSAIVRELQRQGYTNIITRTHKELDLTRQEAVERFFAEEKPEYVFLAAAKVGGIIANQSALADF
MYENMILEMNVIHSAWRNGCRKLEFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNRQYGTDYI
SVMPTNLYGPNDNYHPEHSHVLPALIRRFHEAKEAGLKEVTCWGDGSPLREFLYVDDLANLCVFLMNNYSGYETVNAGTG
KELTIKALTELVAKIIGYTGEIRWDTSRPNGTPRKLLDVSKATSLGWTYKTELEEGIRLSYEDFLNNPMRAER
>Mature_313_residues
MEKNAKIYVAGHRGMVGSAIVRELQRQGYTNIITRTHKELDLTRQEAVERFFAEEKPEYVFLAAAKVGGIIANQSALADF
MYENMILEMNVIHSAWRNGCRKLEFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNRQYGTDYI
SVMPTNLYGPNDNYHPEHSHVLPALIRRFHEAKEAGLKEVTCWGDGSPLREFLYVDDLANLCVFLMNNYSGYETVNAGTG
KELTIKALTELVAKIIGYTGEIRWDTSRPNGTPRKLLDVSKATSLGWTYKTELEEGIRLSYEDFLNNPMRAER

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=285, Percent_Identity=32.9824561403509, Blast_Score=159, Evalue=4e-39,
Organism=Escherichia coli, GI1788365, Length=314, Percent_Identity=52.5477707006369, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI1788353, Length=350, Percent_Identity=25.4285714285714, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17554428, Length=311, Percent_Identity=30.8681672025724, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922778, Length=319, Percent_Identity=32.6018808777429, Blast_Score=165, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 35534; Mature: 35534

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKNAKIYVAGHRGMVGSAIVRELQRQGYTNIITRTHKELDLTRQEAVERFFAEEKPEYV
CCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
FLAAAKVGGIIANQSALADFMYENMILEMNVIHSAWRNGCRKLEFLGSSCIYPRMAPQPM
EEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PESCLLTSELEKTNEAYALAKISGLKYCEFLNRQYGTDYISVMPTNLYGPNDNYHPEHSH
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHH
VLPALIRRFHEAKEAGLKEVTCWGDGSPLREFLYVDDLANLCVFLMNNYSGYETVNAGTG
HHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCC
KELTIKALTELVAKIIGYTGEIRWDTSRPNGTPRKLLDVSKATSLGWTYKTELEEGIRLS
CEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCEEHHHHHHCCEEC
YEDFLNNPMRAER
HHHHCCCCCCCCC
>Mature Secondary Structure
MEKNAKIYVAGHRGMVGSAIVRELQRQGYTNIITRTHKELDLTRQEAVERFFAEEKPEYV
CCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
FLAAAKVGGIIANQSALADFMYENMILEMNVIHSAWRNGCRKLEFLGSSCIYPRMAPQPM
EEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PESCLLTSELEKTNEAYALAKISGLKYCEFLNRQYGTDYISVMPTNLYGPNDNYHPEHSH
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHH
VLPALIRRFHEAKEAGLKEVTCWGDGSPLREFLYVDDLANLCVFLMNNYSGYETVNAGTG
HHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCC
KELTIKALTELVAKIIGYTGEIRWDTSRPNGTPRKLLDVSKATSLGWTYKTELEEGIRLS
CEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCEEHHHHHHCCEEC
YEDFLNNPMRAER
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]