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The map label for this gene is rsmF [H]

Identifier: 150008796

GI number: 150008796

Start: 2572254

End: 2573651

Strand: Direct

Name: rsmF [H]

Synonym: BDI_2186

Alternate gene names: 150008796

Gene position: 2572254-2573651 (Clockwise)

Preceding gene: 150008790

Following gene: 150008800

Centisome position: 53.46

GC content: 51.43

Gene sequence:

>1398_bases
ATGGCACTTCCTATTGATTTTATTACCCGTACACGGGCTTTACTTGGCGAGGAGTTCGATCGTTTAGAGGCAGCGTTAAG
CGCTGATGTTCCCGTAAGTATCCGTATCAACCCGATGAAAGGGACTCCCGCTCCCAAAGCAGGTGCTCCAGTCGCTTGGT
GCGAGAGCGGTTTTTATTTGCCGGAACGGTTGTCTTTCACCTTCGATCCTCTATTCCATGCGGGGGCCTATTACGTGCAG
GAAGCGTCTTCCATGTTTCTGGAACAAGCGATCAAGGCATACGTGAAAGAGCCGGTACGTTGCCTGGATTTATGCGCCGC
CCCGGGTGGCAAGTCCACGCATCTGGCGAGCTTATTGCCGGATGGAAGCCTGTTAGTGAGCAATGAGGTGATCCGCAGCC
GGAGTTATGTATTGGCCGAGAATATCGCTAAATGGGGCCACCCGGACACGATCGTTATCAATAATGACCCGGAGGAGATC
GGAGAGGCATTGCCTCATCTGTTCGACGTGATCGTCACCGACGTACCTTGTTCCGGTGAGGGCATGTTCCGGAAAGATAC
GGATAGCACAGGCGAATGGAGCGTAGATAACGTACGGCTTTGCGCCTCCCGTGGGCGGCGCATCATCCATGATATCTGGA
ACGCCTTGAAACCCGGAGGTATCTTGATCTACAGTACATGTACCTATAATACGGAAGAGGATGAGGAGAACATACATTAT
ATAATAGAGGAGCTGGGAGCCGAGGCATTGCCTATCCCTACCCAAGAGGAATGGCAGATCACCGGCGCCCTCCGGTTTGA
ACATCCGGTTTACCGCTTCTTCCCGCATAAGACACGGGGCGAAGGCTTTTTCCTCGCCGCCCTCCGGAAAGCGGAAGGCG
AGATCGAGGAGATTCGTGTCAAGTCCAAAGGGAAAAAAGAGCGGGGAAAAGCCGCTCCGTCCATCCCACAGGAGGCCCGT
CTGTTCCTTTCGGATGAAACTTCGTTTTCTCTCGAATGGAACGGCAGTTTCCTTCGAGCGTATCCCAAAGCCCATTCGGA
TCTATTTTCACTCATCAATGCCAAGCCTTTACGGGTACTTTCCGCCGGTATTTGCCTAGGAGAAGCGAAAGGGAAAGACT
TTATCCCGTCTCAAGAGCTGGCTTTAAGTACTGCCTTAACTCCCAACGCATTTCCATCCGTGGAACTGGAATGGGAAGAC
GCTATTAAATTCTTAAAGAAAGAGGCGCTCGTCTTACCCTCCGGCATAGATAAAGGATATGTGTTGGTACGCTACCAAAG
ACTTCCGCTAGGTTTTGTAAAGAACCTTGGCAACCGGGCCAATAACCTGTATCCGCAAGAGTGGCGTATACGGACGGGAT
ATATTCCGGAGGAAATCAAACTATTTTTAGGACGATAA

Upstream 100 bases:

>100_bases
TCCGTATCGCCCAAGGTAACTGGTATAAGGAAAAGAGATGCTCGCATACTGTTTGTTTTCCGCGAAAGTAGTATTTTTGC
CGCAAACTAACAGAATAATC

Downstream 100 bases:

>100_bases
GACCACTATATAGGCATAAAGCCACACGTATATACGTATAAGACCACACTTACGTACGTGTAGGCCGATGCGTATATACG
CATGAGGTTACGCCTATATA

Product: tRNA and rRNA cytosine-C5-methylase

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MALPIDFITRTRALLGEEFDRLEAALSADVPVSIRINPMKGTPAPKAGAPVAWCESGFYLPERLSFTFDPLFHAGAYYVQ
EASSMFLEQAIKAYVKEPVRCLDLCAAPGGKSTHLASLLPDGSLLVSNEVIRSRSYVLAENIAKWGHPDTIVINNDPEEI
GEALPHLFDVIVTDVPCSGEGMFRKDTDSTGEWSVDNVRLCASRGRRIIHDIWNALKPGGILIYSTCTYNTEEDEENIHY
IIEELGAEALPIPTQEEWQITGALRFEHPVYRFFPHKTRGEGFFLAALRKAEGEIEEIRVKSKGKKERGKAAPSIPQEAR
LFLSDETSFSLEWNGSFLRAYPKAHSDLFSLINAKPLRVLSAGICLGEAKGKDFIPSQELALSTALTPNAFPSVELEWED
AIKFLKKEALVLPSGIDKGYVLVRYQRLPLGFVKNLGNRANNLYPQEWRIRTGYIPEEIKLFLGR

Sequences:

>Translated_465_residues
MALPIDFITRTRALLGEEFDRLEAALSADVPVSIRINPMKGTPAPKAGAPVAWCESGFYLPERLSFTFDPLFHAGAYYVQ
EASSMFLEQAIKAYVKEPVRCLDLCAAPGGKSTHLASLLPDGSLLVSNEVIRSRSYVLAENIAKWGHPDTIVINNDPEEI
GEALPHLFDVIVTDVPCSGEGMFRKDTDSTGEWSVDNVRLCASRGRRIIHDIWNALKPGGILIYSTCTYNTEEDEENIHY
IIEELGAEALPIPTQEEWQITGALRFEHPVYRFFPHKTRGEGFFLAALRKAEGEIEEIRVKSKGKKERGKAAPSIPQEAR
LFLSDETSFSLEWNGSFLRAYPKAHSDLFSLINAKPLRVLSAGICLGEAKGKDFIPSQELALSTALTPNAFPSVELEWED
AIKFLKKEALVLPSGIDKGYVLVRYQRLPLGFVKNLGNRANNLYPQEWRIRTGYIPEEIKLFLGR
>Mature_464_residues
ALPIDFITRTRALLGEEFDRLEAALSADVPVSIRINPMKGTPAPKAGAPVAWCESGFYLPERLSFTFDPLFHAGAYYVQE
ASSMFLEQAIKAYVKEPVRCLDLCAAPGGKSTHLASLLPDGSLLVSNEVIRSRSYVLAENIAKWGHPDTIVINNDPEEIG
EALPHLFDVIVTDVPCSGEGMFRKDTDSTGEWSVDNVRLCASRGRRIIHDIWNALKPGGILIYSTCTYNTEEDEENIHYI
IEELGAEALPIPTQEEWQITGALRFEHPVYRFFPHKTRGEGFFLAALRKAEGEIEEIRVKSKGKKERGKAAPSIPQEARL
FLSDETSFSLEWNGSFLRAYPKAHSDLFSLINAKPLRVLSAGICLGEAKGKDFIPSQELALSTALTPNAFPSVELEWEDA
IKFLKKEALVLPSGIDKGYVLVRYQRLPLGFVKNLGNRANNLYPQEWRIRTGYIPEEIKLFLGR

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=261, Percent_Identity=28.3524904214559, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI76150623, Length=261, Percent_Identity=28.3524904214559, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI301336155, Length=187, Percent_Identity=32.0855614973262, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI39995082, Length=187, Percent_Identity=32.0855614973262, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI32698918, Length=194, Percent_Identity=32.9896907216495, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI23199998, Length=248, Percent_Identity=27.0161290322581, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI270288818, Length=248, Percent_Identity=27.0161290322581, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI8922322, Length=248, Percent_Identity=27.0161290322581, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI270288816, Length=248, Percent_Identity=27.0161290322581, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI11545785, Length=175, Percent_Identity=31.4285714285714, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI87081985, Length=484, Percent_Identity=30.1652892561983, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI2367212, Length=222, Percent_Identity=28.8288288288288, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17536757, Length=319, Percent_Identity=28.8401253918495, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI25143471, Length=279, Percent_Identity=25.4480286738351, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=284, Percent_Identity=29.2253521126761, Blast_Score=106, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6319447, Length=281, Percent_Identity=25.6227758007117, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI22024126, Length=299, Percent_Identity=28.7625418060201, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21355201, Length=193, Percent_Identity=32.1243523316062, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24668781, Length=226, Percent_Identity=28.7610619469027, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21356513, Length=226, Percent_Identity=28.7610619469027, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 51912; Mature: 51781

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALPIDFITRTRALLGEEFDRLEAALSADVPVSIRINPMKGTPAPKAGAPVAWCESGFYL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCC
PERLSFTFDPLFHAGAYYVQEASSMFLEQAIKAYVKEPVRCLDLCAAPGGKSTHLASLLP
HHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCC
DGSLLVSNEVIRSRSYVLAENIAKWGHPDTIVINNDPEEIGEALPHLFDVIVTDVPCSGE
CCCEEECHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCC
GMFRKDTDSTGEWSVDNVRLCASRGRRIIHDIWNALKPGGILIYSTCTYNTEEDEENIHY
CCEECCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHH
IIEELGAEALPIPTQEEWQITGALRFEHPVYRFFPHKTRGEGFFLAALRKAEGEIEEIRV
HHHHHCCCCCCCCCCCCEEEEEEEEECCCHHHHCCCCCCCCEEEEEEHHHCCCCHHHHEE
KSKGKKERGKAAPSIPQEARLFLSDETSFSLEWNGSFLRAYPKAHSDLFSLINAKPLRVL
CCCCCHHHCCCCCCCCHHHEEEEECCCEEEEEECCCEEEECCHHHHHHHHHHCCCHHHHH
SAGICLGEAKGKDFIPSQELALSTALTPNAFPSVELEWEDAIKFLKKEALVLPSGIDKGY
HHCEEEECCCCCCCCCCHHHHEEECCCCCCCCCEEECHHHHHHHHHHHHEECCCCCCCCE
VLVRYQRLPLGFVKNLGNRANNLYPQEWRIRTGYIPEEIKLFLGR
EEEEEECCCHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHCCCC
>Mature Secondary Structure 
ALPIDFITRTRALLGEEFDRLEAALSADVPVSIRINPMKGTPAPKAGAPVAWCESGFYL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCC
PERLSFTFDPLFHAGAYYVQEASSMFLEQAIKAYVKEPVRCLDLCAAPGGKSTHLASLLP
HHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCC
DGSLLVSNEVIRSRSYVLAENIAKWGHPDTIVINNDPEEIGEALPHLFDVIVTDVPCSGE
CCCEEECHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCC
GMFRKDTDSTGEWSVDNVRLCASRGRRIIHDIWNALKPGGILIYSTCTYNTEEDEENIHY
CCEECCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHH
IIEELGAEALPIPTQEEWQITGALRFEHPVYRFFPHKTRGEGFFLAALRKAEGEIEEIRV
HHHHHCCCCCCCCCCCCEEEEEEEEECCCHHHHCCCCCCCCEEEEEEHHHCCCCHHHHEE
KSKGKKERGKAAPSIPQEARLFLSDETSFSLEWNGSFLRAYPKAHSDLFSLINAKPLRVL
CCCCCHHHCCCCCCCCHHHEEEEECCCEEEEEECCCEEEECCHHHHHHHHHHCCCHHHHH
SAGICLGEAKGKDFIPSQELALSTALTPNAFPSVELEWEDAIKFLKKEALVLPSGIDKGY
HHCEEEECCCCCCCCCCHHHHEEECCCCCCCCCEEECHHHHHHHHHHHHEECCCCCCCCE
VLVRYQRLPLGFVKNLGNRANNLYPQEWRIRTGYIPEEIKLFLGR
EEEEEECCCHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA