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The map label for this gene is cca [H]

Identifier: 150008663

GI number: 150008663

Start: 2421550

End: 2422980

Strand: Reverse

Name: cca [H]

Synonym: BDI_2053

Alternate gene names: 150008663

Gene position: 2422980-2421550 (Counterclockwise)

Preceding gene: 150008665

Following gene: 150008661

Centisome position: 50.36

GC content: 53.18

Gene sequence:

>1431_bases
ATGTATATAAACGAGGAAGAGATAGTAAATCATATATCCGCGAAGCCTTTCCATTTGATATCGGAGGCGGCGGAAGAGCT
AGGTGTTGAGGTATATGTAATAGGCGGATATGTGCGGGATATTTTCTTGAACCGCCCATCCAAGGATATAGACGTGGTAG
CGGTTGGCAGCGGTATCGAGTTGGCCAAACGAGTGGCCAAAAAGATCGGGCGTGGCGCTTATCTTTCCGTCTTCAAGAAT
TTCGGGACGGCGCAGGTGAAAGCCGGCGATCTGGAGCTGGAATTCGTGGGGGCGAGGAAAGAATCCTACAGCCATGACAG
CCGTAAGCCTGTCGTGGAAGACGGCACCTTGGAGGATGACCAGAACCGCCGCGATTTCACGATCAACGCCTTGGCCCTTT
GCCTGAACAAGGAGCGTTACGGAGAATTGGTCGATCCCTTCGGCGGGTTGGAGGATATGGACGAGCTTACGATCCGTACG
CCTTTAGACCCGGATGTTACTTTCAGCGATGATCCGCTCCGGATGATGCGTGCGGTTCGTTTCGCCACTCAATTAGGTTT
CTTTATCGATCCCGACACCTTCGACGCCATCATCCGTAACCGGGAGCGTATCTCCATCATCTCCAAGGAGCGTATCGTGG
ACGAGTTGAACAAGATCGTCCTCTCGCCAAAGCCCTCTATCGGTTTCGACTTGCTGGATAAATGCGGACTGTTGCCATTG
ATTTTCCCCGAGCTTTGCGCCTTGAAGGGCGTGGAGACCAAGGAGGGGATCGGGCATAAGGATAATTTCGCCCATACCTT
GATGGTGCTGGATCGTTTGAGCAAGACTTCCGATAATCTATGGCTGCGTTGGAGCGCCCTCTTCCACGATATCGCCAAGC
CTGCCACGAAGCGTTTCGACCCTCGCTTGGGATGGACGTTCCATAATCATAATTTTATCGGCGAGCGGATGATCCCCGGT
ATTTTCAGGAAGATGAAACTCCCGATGAATGAGAAGATGAAGTACGTGCAGAAGATGGTAAGCCTGCATATGCGTCCGAT
CGCCTTGTCGGACGATGAGGTGACGGATTCGGCGATCCGCCGTCTGCTGTTTGACGCGGGAGATGATATCGACGATTTGA
TGAAACTGTGCGAGGCCGATATCACGAGCAAGAACCCGGAGAAGGTTCGCCGTTTTTTGAACAACTTTCGAAAGGTGCGC
GAGAAGCTGGCCGATATAGAGGAAAAAGACCGTGTCCGTAACTTCCAGCCCCCGGTATCCGGCGAGGAGATCATGGAGAC
GTTTGGCTTGGCTCCCTCCAAGCCTGTCGGCGTGCTGAAAGAGGCGATCAAGAACGCCATCCTCGACGGCGTGATCCCGA
ACGAGTATGAGGCGGCCCGTCAATTCCTTATACAACGTGCGGAGAAGATGGGGCTGAAGCCGATTCTGTGA

Upstream 100 bases:

>100_bases
TAACTTTTTCATATCTTTCTCGTTCAATGTATTTGATACCGCAAAAGTACATGAAATTTGATTATCTTTGCGCTCTTTAT
TGGATTTGGAATACATAAAT

Downstream 100 bases:

>100_bases
TCTTATAGTCACAAGGCTCAGTCTTACAGTTGGCACAGTCCCCTGTACGAAGTACTGCGGTACTTCCTAGAGGGTACTGT
AGTACTTCGTACAGAGTACT

Product: tRNA nucleotidyltransferase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MYINEEEIVNHISAKPFHLISEAAEELGVEVYVIGGYVRDIFLNRPSKDIDVVAVGSGIELAKRVAKKIGRGAYLSVFKN
FGTAQVKAGDLELEFVGARKESYSHDSRKPVVEDGTLEDDQNRRDFTINALALCLNKERYGELVDPFGGLEDMDELTIRT
PLDPDVTFSDDPLRMMRAVRFATQLGFFIDPDTFDAIIRNRERISIISKERIVDELNKIVLSPKPSIGFDLLDKCGLLPL
IFPELCALKGVETKEGIGHKDNFAHTLMVLDRLSKTSDNLWLRWSALFHDIAKPATKRFDPRLGWTFHNHNFIGERMIPG
IFRKMKLPMNEKMKYVQKMVSLHMRPIALSDDEVTDSAIRRLLFDAGDDIDDLMKLCEADITSKNPEKVRRFLNNFRKVR
EKLADIEEKDRVRNFQPPVSGEEIMETFGLAPSKPVGVLKEAIKNAILDGVIPNEYEAARQFLIQRAEKMGLKPIL

Sequences:

>Translated_476_residues
MYINEEEIVNHISAKPFHLISEAAEELGVEVYVIGGYVRDIFLNRPSKDIDVVAVGSGIELAKRVAKKIGRGAYLSVFKN
FGTAQVKAGDLELEFVGARKESYSHDSRKPVVEDGTLEDDQNRRDFTINALALCLNKERYGELVDPFGGLEDMDELTIRT
PLDPDVTFSDDPLRMMRAVRFATQLGFFIDPDTFDAIIRNRERISIISKERIVDELNKIVLSPKPSIGFDLLDKCGLLPL
IFPELCALKGVETKEGIGHKDNFAHTLMVLDRLSKTSDNLWLRWSALFHDIAKPATKRFDPRLGWTFHNHNFIGERMIPG
IFRKMKLPMNEKMKYVQKMVSLHMRPIALSDDEVTDSAIRRLLFDAGDDIDDLMKLCEADITSKNPEKVRRFLNNFRKVR
EKLADIEEKDRVRNFQPPVSGEEIMETFGLAPSKPVGVLKEAIKNAILDGVIPNEYEAARQFLIQRAEKMGLKPIL
>Mature_476_residues
MYINEEEIVNHISAKPFHLISEAAEELGVEVYVIGGYVRDIFLNRPSKDIDVVAVGSGIELAKRVAKKIGRGAYLSVFKN
FGTAQVKAGDLELEFVGARKESYSHDSRKPVVEDGTLEDDQNRRDFTINALALCLNKERYGELVDPFGGLEDMDELTIRT
PLDPDVTFSDDPLRMMRAVRFATQLGFFIDPDTFDAIIRNRERISIISKERIVDELNKIVLSPKPSIGFDLLDKCGLLPL
IFPELCALKGVETKEGIGHKDNFAHTLMVLDRLSKTSDNLWLRWSALFHDIAKPATKRFDPRLGWTFHNHNFIGERMIPG
IFRKMKLPMNEKMKYVQKMVSLHMRPIALSDDEVTDSAIRRLLFDAGDDIDDLMKLCEADITSKNPEKVRRFLNNFRKVR
EKLADIEEKDRVRNFQPPVSGEEIMETFGLAPSKPVGVLKEAIKNAILDGVIPNEYEAARQFLIQRAEKMGLKPIL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=327, Percent_Identity=31.8042813455657, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI87081691, Length=231, Percent_Identity=29.004329004329, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71995920, Length=229, Percent_Identity=28.82096069869, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321016, Length=232, Percent_Identity=35.3448275862069, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281360112, Length=216, Percent_Identity=29.1666666666667, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21357337, Length=216, Percent_Identity=29.1666666666667, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 54105; Mature: 54105

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYINEEEIVNHISAKPFHLISEAAEELGVEVYVIGGYVRDIFLNRPSKDIDVVAVGSGIE
CCCCHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECHHHHHHHHCCCCCCCEEEEECCCHH
LAKRVAKKIGRGAYLSVFKNFGTAQVKAGDLELEFVGARKESYSHDSRKPVVEDGTLEDD
HHHHHHHHHCCCHHHHHHHCCCCCEEEECCEEEEEECCCHHHCCCCCCCCCCCCCCCCCC
QNRRDFTINALALCLNKERYGELVDPFGGLEDMDELTIRTPLDPDVTFSDDPLRMMRAVR
CCCCCCHHHHHHHHHCHHHHHHHHCCCCCCCCHHHCEEECCCCCCCCCCCCHHHHHHHHH
FATQLGFFIDPDTFDAIIRNRERISIISKERIVDELNKIVLSPKPSIGFDLLDKCGLLPL
HHHHHCCEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHH
IFPELCALKGVETKEGIGHKDNFAHTLMVLDRLSKTSDNLWLRWSALFHDIAKPATKRFD
HHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCC
PRLGWTFHNHNFIGERMIPGIFRKMKLPMNEKMKYVQKMVSLHMRPIALSDDEVTDSAIR
CCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHH
RLLFDAGDDIDDLMKLCEADITSKNPEKVRRFLNNFRKVREKLADIEEKDRVRNFQPPVS
HHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC
GEEIMETFGLAPSKPVGVLKEAIKNAILDGVIPNEYEAARQFLIQRAEKMGLKPIL
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MYINEEEIVNHISAKPFHLISEAAEELGVEVYVIGGYVRDIFLNRPSKDIDVVAVGSGIE
CCCCHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECHHHHHHHHCCCCCCCEEEEECCCHH
LAKRVAKKIGRGAYLSVFKNFGTAQVKAGDLELEFVGARKESYSHDSRKPVVEDGTLEDD
HHHHHHHHHCCCHHHHHHHCCCCCEEEECCEEEEEECCCHHHCCCCCCCCCCCCCCCCCC
QNRRDFTINALALCLNKERYGELVDPFGGLEDMDELTIRTPLDPDVTFSDDPLRMMRAVR
CCCCCCHHHHHHHHHCHHHHHHHHCCCCCCCCHHHCEEECCCCCCCCCCCCHHHHHHHHH
FATQLGFFIDPDTFDAIIRNRERISIISKERIVDELNKIVLSPKPSIGFDLLDKCGLLPL
HHHHHCCEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHH
IFPELCALKGVETKEGIGHKDNFAHTLMVLDRLSKTSDNLWLRWSALFHDIAKPATKRFD
HHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCC
PRLGWTFHNHNFIGERMIPGIFRKMKLPMNEKMKYVQKMVSLHMRPIALSDDEVTDSAIR
CCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHH
RLLFDAGDDIDDLMKLCEADITSKNPEKVRRFLNNFRKVREKLADIEEKDRVRNFQPPVS
HHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC
GEEIMETFGLAPSKPVGVLKEAIKNAILDGVIPNEYEAARQFLIQRAEKMGLKPIL
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA