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The map label for this gene is gacS [H]

Identifier: 150008613

GI number: 150008613

Start: 2364652

End: 2366958

Strand: Direct

Name: gacS [H]

Synonym: BDI_1999

Alternate gene names: 150008613

Gene position: 2364652-2366958 (Clockwise)

Preceding gene: 150008612

Following gene: 150008618

Centisome position: 49.15

GC content: 43.91

Gene sequence:

>2307_bases
ATGGCGAACACTTCTTATCGTTATCTTTTTTTATTGTTGCTTCCATTATTTCTATTGGTCGCAAATTCTTGCGAACGCTC
AACCACGGAAAAGCGTATCTTGATAGTCCAATCTTATGAGCCGGACTTTCAAGCCTATAAAGATATAGAAGAGGCTTTTA
AAAAAGGATTCCAGAAGGAAGGCATTCCTGCTAGTATCTTCACGTTCTATCTTAATTGCGAAGCTTATCAATCGCTAGAA
GAGAAACAAAGAATATATACGGAACTCAATACGTTATCTTTATGGAAGCCTGATATCATAATCGTAAACGATGATCAAGC
GACCTATTCCTTATTGGCTTGCGAACATCCTTTATTAGATAGCGTACCTATCGTTTTTACCGGAGTCAACTACCCAAACA
TACCCTTAATTCAAAAGTATCCTAACGTATCCGGATTTTGGGATAAGCCGGACTATCGGAAAAACGTCGAACTTATCGAG
CGAATCATGGGAAAATGCGTAATTGTACGTGTCTCTGATAGTACAGCTCTTGACAAAAAGATCCTGAAAGATATGGATGA
GCAAATAAAAGGGCTATGCTCCAAGGCTAGACCCGATTACCTTAAATATCCCCAGTATTCATCCCCATCCGATAAAAAGA
GATCCTCTTCTTTAGTGCGGTTCCCAAAAGTACCGTTCGATTCCCTCTATATACAAACCATTCAACCCCGCACCAGTTCC
AACTTGATCTGGGGGTTAGGTACAAATACTTACAACAAGGCCTACCTTGCTACCAAAAGAGATTATACCTCGATAGCCTT
AGGTAGGTTCTGCTCATTTCCCAGCTTCTCGGCGATCAACGAATCCGTAGGATATGACGGAGACTTCATCGGAGGATATA
TGACCCCGGTAGAGAGCCAAACCCAAGAAGCCTTACGACGAGCTGCCAGCATACTCAAGGGCACACCGGCCAATAGCTTC
CCACAAATAACGGAAAGCGCCAAAAACTATCTGTTCGATTACCCGACTTTAAATAAATGGGGTATCGATTGGAAAGAACT
GCCTCAAAACAGTATCTTCTTGAATATGCCCTTTGTGGTTCGTTATCAAACATATATTATTCTATGCGGGATTCTTCTAA
CCCTATTTATCCTATGGACACTTTTCTATCAAAGAGTACAATACCGTCGCGAAGCCTCTCACAAGAAACAGGCTCAAGAA
AGTTTACGGAAAGAGAAAGAATTCCTCTCGTTGGCTTTAGAGAGCGGTGATATTTTCGCATTCCGATACAGCAACGGCGT
ATTCGAGTTCGACCATGATTTCTATAAGTCGTTGGATATGCCGATAAAGCCGATCACCTCCACGCAATTCCAAGAGTCTA
TCCACCCTGAAGACCGGGAAGATTTCATCCAACATAAGCACCTGCTTGATACAGGATTCCCTTCCCGTAAGATCACCCGC
CGCCGATATAATTTCAACGGCAAAGGGCATATATGGTGGGAATTCAGATATGCGCAAGCGAAGAATGGACAAGACTCCAC
CCGCAATAATGTCGGAGTAAACGGGCTCTGCTTAAATATCCAACAAAGTAAGGAAGTAGAGGAAGATCTTATCAAAGCCC
GTATAAGGGCCGAGGAAGCGGACCAAATGAAATCAGCGTTCCTCGCCAATATGAGCCATGAGATACGTACCCCGCTTAAC
GCCATCGTTGGATTTTCGGAACTGCTAACAAGCGATATGGAAATAAGCCCGAACGAGAGGGATGAGTTCATGCAAGTTAT
CAGTAAGAACAGCGACCTCTTATTGAAATTGATCAATGACATTCTCGATCTATCCCGCATCAAGTCCGGTAAGATGTCGT
TTACCCTCACGAATTGTGACTTAAACGAATTACTATCCCATATCTACCGCACCCATCAATTGTTGATGCCCCAAGGCGTA
GCGCTTAAAATACAACTGCCGGTCCTCCCGGCCATTATCCAAGCGGACCTACATCGATTAACCCAAGTGATTACCAACCT
CATAAACAACGCCGCAAAATTCACGACGCAAGGATACATCTTGATTAAATACGATTATACGCAAGATAAACGTTGGATCC
AGATCTCCGTGACCGATACGGGCAAAGGCATTCCCGCAGATAAGCAAGCCCAAGTATTCGAACGGTTCAACAAACTGGAC
GAGTTTGCGCAAGGGACAGGCCTCGGACTGGCGATCTGCCAAATTATCATCGAGCACTTCGGAGGATATATCACCCTAGA
ATCCAAAGAAGGGGAAGGTAGCACATTCATCGTGACCCTTCCCTACACTCCCGGATTATCGGTATAA

Upstream 100 bases:

>100_bases
ATTATAAACCAACCTCAGAAGCCAAGAAAAATAGACATTTGTCTCTTTGATATCCAATTACTTTTTATACATTTGTACCT
ATTATATTGAAGCTATACAC

Downstream 100 bases:

>100_bases
GATTATTTTTTCTTGAAACGCAAGAACGGCAATACAATAAATAAAGGTATGCAACAGATCATTACCCAGATAAAGAAATG
TTGGTAACCGATCTGCTCCT

Product: putative two-component regulator sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 768; Mature: 767

Protein sequence:

>768_residues
MANTSYRYLFLLLLPLFLLVANSCERSTTEKRILIVQSYEPDFQAYKDIEEAFKKGFQKEGIPASIFTFYLNCEAYQSLE
EKQRIYTELNTLSLWKPDIIIVNDDQATYSLLACEHPLLDSVPIVFTGVNYPNIPLIQKYPNVSGFWDKPDYRKNVELIE
RIMGKCVIVRVSDSTALDKKILKDMDEQIKGLCSKARPDYLKYPQYSSPSDKKRSSSLVRFPKVPFDSLYIQTIQPRTSS
NLIWGLGTNTYNKAYLATKRDYTSIALGRFCSFPSFSAINESVGYDGDFIGGYMTPVESQTQEALRRAASILKGTPANSF
PQITESAKNYLFDYPTLNKWGIDWKELPQNSIFLNMPFVVRYQTYIILCGILLTLFILWTLFYQRVQYRREASHKKQAQE
SLRKEKEFLSLALESGDIFAFRYSNGVFEFDHDFYKSLDMPIKPITSTQFQESIHPEDREDFIQHKHLLDTGFPSRKITR
RRYNFNGKGHIWWEFRYAQAKNGQDSTRNNVGVNGLCLNIQQSKEVEEDLIKARIRAEEADQMKSAFLANMSHEIRTPLN
AIVGFSELLTSDMEISPNERDEFMQVISKNSDLLLKLINDILDLSRIKSGKMSFTLTNCDLNELLSHIYRTHQLLMPQGV
ALKIQLPVLPAIIQADLHRLTQVITNLINNAAKFTTQGYILIKYDYTQDKRWIQISVTDTGKGIPADKQAQVFERFNKLD
EFAQGTGLGLAICQIIIEHFGGYITLESKEGEGSTFIVTLPYTPGLSV

Sequences:

>Translated_768_residues
MANTSYRYLFLLLLPLFLLVANSCERSTTEKRILIVQSYEPDFQAYKDIEEAFKKGFQKEGIPASIFTFYLNCEAYQSLE
EKQRIYTELNTLSLWKPDIIIVNDDQATYSLLACEHPLLDSVPIVFTGVNYPNIPLIQKYPNVSGFWDKPDYRKNVELIE
RIMGKCVIVRVSDSTALDKKILKDMDEQIKGLCSKARPDYLKYPQYSSPSDKKRSSSLVRFPKVPFDSLYIQTIQPRTSS
NLIWGLGTNTYNKAYLATKRDYTSIALGRFCSFPSFSAINESVGYDGDFIGGYMTPVESQTQEALRRAASILKGTPANSF
PQITESAKNYLFDYPTLNKWGIDWKELPQNSIFLNMPFVVRYQTYIILCGILLTLFILWTLFYQRVQYRREASHKKQAQE
SLRKEKEFLSLALESGDIFAFRYSNGVFEFDHDFYKSLDMPIKPITSTQFQESIHPEDREDFIQHKHLLDTGFPSRKITR
RRYNFNGKGHIWWEFRYAQAKNGQDSTRNNVGVNGLCLNIQQSKEVEEDLIKARIRAEEADQMKSAFLANMSHEIRTPLN
AIVGFSELLTSDMEISPNERDEFMQVISKNSDLLLKLINDILDLSRIKSGKMSFTLTNCDLNELLSHIYRTHQLLMPQGV
ALKIQLPVLPAIIQADLHRLTQVITNLINNAAKFTTQGYILIKYDYTQDKRWIQISVTDTGKGIPADKQAQVFERFNKLD
EFAQGTGLGLAICQIIIEHFGGYITLESKEGEGSTFIVTLPYTPGLSV
>Mature_767_residues
ANTSYRYLFLLLLPLFLLVANSCERSTTEKRILIVQSYEPDFQAYKDIEEAFKKGFQKEGIPASIFTFYLNCEAYQSLEE
KQRIYTELNTLSLWKPDIIIVNDDQATYSLLACEHPLLDSVPIVFTGVNYPNIPLIQKYPNVSGFWDKPDYRKNVELIER
IMGKCVIVRVSDSTALDKKILKDMDEQIKGLCSKARPDYLKYPQYSSPSDKKRSSSLVRFPKVPFDSLYIQTIQPRTSSN
LIWGLGTNTYNKAYLATKRDYTSIALGRFCSFPSFSAINESVGYDGDFIGGYMTPVESQTQEALRRAASILKGTPANSFP
QITESAKNYLFDYPTLNKWGIDWKELPQNSIFLNMPFVVRYQTYIILCGILLTLFILWTLFYQRVQYRREASHKKQAQES
LRKEKEFLSLALESGDIFAFRYSNGVFEFDHDFYKSLDMPIKPITSTQFQESIHPEDREDFIQHKHLLDTGFPSRKITRR
RYNFNGKGHIWWEFRYAQAKNGQDSTRNNVGVNGLCLNIQQSKEVEEDLIKARIRAEEADQMKSAFLANMSHEIRTPLNA
IVGFSELLTSDMEISPNERDEFMQVISKNSDLLLKLINDILDLSRIKSGKMSFTLTNCDLNELLSHIYRTHQLLMPQGVA
LKIQLPVLPAIIQADLHRLTQVITNLINNAAKFTTQGYILIKYDYTQDKRWIQISVTDTGKGIPADKQAQVFERFNKLDE
FAQGTGLGLAICQIIIEHFGGYITLESKEGEGSTFIVTLPYTPGLSV

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=247, Percent_Identity=38.8663967611336, Blast_Score=149, Evalue=9e-37,
Organism=Escherichia coli, GI145693157, Length=258, Percent_Identity=37.5968992248062, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI87081816, Length=248, Percent_Identity=35.8870967741936, Blast_Score=133, Evalue=4e-32,
Organism=Escherichia coli, GI1788713, Length=427, Percent_Identity=28.1030444964871, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI48994928, Length=264, Percent_Identity=31.4393939393939, Blast_Score=126, Evalue=6e-30,
Organism=Escherichia coli, GI1790436, Length=218, Percent_Identity=36.2385321100917, Blast_Score=116, Evalue=7e-27,
Organism=Escherichia coli, GI1786912, Length=238, Percent_Identity=32.7731092436975, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1788393, Length=232, Percent_Identity=28.448275862069, Blast_Score=88, Evalue=3e-18,
Organism=Escherichia coli, GI1788549, Length=237, Percent_Identity=30.379746835443, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786600, Length=223, Percent_Identity=25.1121076233184, Blast_Score=84, Evalue=5e-17,
Organism=Escherichia coli, GI1790346, Length=220, Percent_Identity=27.7272727272727, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790861, Length=274, Percent_Identity=27.007299270073, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI87082128, Length=235, Percent_Identity=27.6595744680851, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1789808, Length=223, Percent_Identity=26.0089686098655, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI1786783, Length=234, Percent_Identity=25.2136752136752, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1787894, Length=225, Percent_Identity=26.2222222222222, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1790551, Length=231, Percent_Identity=26.8398268398268, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 88171; Mature: 88040

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANTSYRYLFLLLLPLFLLVANSCERSTTEKRILIVQSYEPDFQAYKDIEEAFKKGFQKE
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCC
GIPASIFTFYLNCEAYQSLEEKQRIYTELNTLSLWKPDIIIVNDDQATYSLLACEHPLLD
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCEEHEECCCCCCC
SVPIVFTGVNYPNIPLIQKYPNVSGFWDKPDYRKNVELIERIMGKCVIVRVSDSTALDKK
CCCEEEECCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEEECCCHHHHHH
ILKDMDEQIKGLCSKARPDYLKYPQYSSPSDKKRSSSLVRFPKVPFDSLYIQTIQPRTSS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCEECCCCCCCCEEEEEECCCCCC
NLIWGLGTNTYNKAYLATKRDYTSIALGRFCSFPSFSAINESVGYDGDFIGGYMTPVESQ
CEEEECCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCHH
TQEALRRAASILKGTPANSFPQITESAKNYLFDYPTLNKWGIDWKELPQNSIFLNMPFVV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEECCCCCCCCCCCHHHCCCCCEEEECCHHH
RYQTYIILCGILLTLFILWTLFYQRVQYRREASHKKQAQESLRKEKEFLSLALESGDIFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
FRYSNGVFEFDHDFYKSLDMPIKPITSTQFQESIHPEDREDFIQHKHLLDTGFPSRKITR
EEECCCEEEECCHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHH
RRYNFNGKGHIWWEFRYAQAKNGQDSTRNNVGVNGLCLNIQQSKEVEEDLIKARIRAEEA
HHCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHH
DQMKSAFLANMSHEIRTPLNAIVGFSELLTSDMEISPNERDEFMQVISKNSDLLLKLIND
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
ILDLSRIKSGKMSFTLTNCDLNELLSHIYRTHQLLMPQGVALKIQLPVLPAIIQADLHRL
HHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHH
TQVITNLINNAAKFTTQGYILIKYDYTQDKRWIQISVTDTGKGIPADKQAQVFERFNKLD
HHHHHHHHHHHHHHEECCEEEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
EFAQGTGLGLAICQIIIEHFGGYITLESKEGEGSTFIVTLPYTPGLSV
HHHCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
ANTSYRYLFLLLLPLFLLVANSCERSTTEKRILIVQSYEPDFQAYKDIEEAFKKGFQKE
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCC
GIPASIFTFYLNCEAYQSLEEKQRIYTELNTLSLWKPDIIIVNDDQATYSLLACEHPLLD
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCEEHEECCCCCCC
SVPIVFTGVNYPNIPLIQKYPNVSGFWDKPDYRKNVELIERIMGKCVIVRVSDSTALDKK
CCCEEEECCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEEECCCHHHHHH
ILKDMDEQIKGLCSKARPDYLKYPQYSSPSDKKRSSSLVRFPKVPFDSLYIQTIQPRTSS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCEECCCCCCCCEEEEEECCCCCC
NLIWGLGTNTYNKAYLATKRDYTSIALGRFCSFPSFSAINESVGYDGDFIGGYMTPVESQ
CEEEECCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCHH
TQEALRRAASILKGTPANSFPQITESAKNYLFDYPTLNKWGIDWKELPQNSIFLNMPFVV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEECCCCCCCCCCCHHHCCCCCEEEECCHHH
RYQTYIILCGILLTLFILWTLFYQRVQYRREASHKKQAQESLRKEKEFLSLALESGDIFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
FRYSNGVFEFDHDFYKSLDMPIKPITSTQFQESIHPEDREDFIQHKHLLDTGFPSRKITR
EEECCCEEEECCHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHH
RRYNFNGKGHIWWEFRYAQAKNGQDSTRNNVGVNGLCLNIQQSKEVEEDLIKARIRAEEA
HHCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHH
DQMKSAFLANMSHEIRTPLNAIVGFSELLTSDMEISPNERDEFMQVISKNSDLLLKLIND
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
ILDLSRIKSGKMSFTLTNCDLNELLSHIYRTHQLLMPQGVALKIQLPVLPAIIQADLHRL
HHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHH
TQVITNLINNAAKFTTQGYILIKYDYTQDKRWIQISVTDTGKGIPADKQAQVFERFNKLD
HHHHHHHHHHHHHHEECCEEEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
EFAQGTGLGLAICQIIIEHFGGYITLESKEGEGSTFIVTLPYTPGLSV
HHHCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]