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The map label for this gene is gacS [H]

Identifier: 150008612

GI number: 150008612

Start: 2362642

End: 2364558

Strand: Direct

Name: gacS [H]

Synonym: BDI_1998

Alternate gene names: 150008612

Gene position: 2362642-2364558 (Clockwise)

Preceding gene: 150008611

Following gene: 150008613

Centisome position: 49.11

GC content: 42.62

Gene sequence:

>1917_bases
ATGCTAGCGAGATCTATACAGAAATATTACATATTTGTTCTACTTTTTCTGCTAGCCATTCTACAGTCCTCTTGTCAACA
GTCGGAAAATAAATATCGTATATTAGTAGTCCATTCCTATGAAAGCGACTATGTAGCTTATAAAGACTGCGATCGGTTGA
TCCGGAAATCCCTCGAGAAAAAGGGGATTAATCCGTCGATCCAAACATTCTATTTGAACTGCGAGCAATATGCGGCACCT
GCCGAAGAAAAACGGATGTACCTGTATCTAGATTCGATCTCCACTTGGAAACCCGATCTGATATTGGTCTACGAGGATCA
AGCGACTTACACGTTAATGCAATGCCATCATCCGCTAATTTCTACAATTCCCATTGTATTTGGCGGCGTAAATTTCCCCA
ATAAGGCACTATTGGCGCAATATTCGAATGTTTCCGGATTCTGGGACGAGCCCGACTACGTCACGAACATCCGCTTGATA
GAACATCTGTTGGGCAAGTCTACGATTTATATGTTGCACGATAGTACGTATATAGATAGGCACATTAAGGCTACGTTACA
TGAGCAATGTGCTCAAGCAGACATTCGGGTCGATAATAATCGCATCATGTATATACCGGTAGAGATCGCTACTTTGGACA
GGGTCAATCAAAGCTTAAAACGACCGGATAGTACGACCGTCAACGTCGTGCCCGTGCAAGGAGACAAGCTATCAGCTGTT
TCTTGGTACATGAGTAAACACGTCCCTTACATCTACTATCTACAGGCCAAACGGGATTACCGGGTATTGAACACCAGCCG
TTTTTCCAGTAAACCCTCTTTTACGGCGATCAACGAGGATCTAGGATATACGAATAAACTTGTAGGTGGCTATATCACCT
CCTTGGAAACCCAAATAGAAGAATCTACCGATAGAGCCGCGGAAATATTAAAAGGCTCCCCTAGCGAATCATTCCCACAG
ATAACAAAGAGCAAGAAGAATTATGTATTTGATTTCAATGAAGTCAAATATTGGAATATCGATAAAAGGCTTCTTCCGAA
GGATGCTATACTTTTGAACTTTCCTTTTAAGGACCAATATCCAAGATTATTCTGGAGCTTGATAATCGTAGTCAGCACGA
TGTGCTGCTTTGTCTTCGGTAGCTTCATCATCATGTATACCCAAGAATCCAAGGCAAAACGACAAGCGCAAAAAGCCCTA
TTACATGAAAAGGAATCATTAGCGGAAGCCTTGGAGAAAGCCAATGAGTCCGACCGGATGAAATCCGTATTCTTGGCTAA
CATGAGTCATGAGATACGTACCCCTCTAAATGCGATCATCGGCTTTTCAAGCCTAATCGCCGAACTGGATCTGACAGCGG
AAGAGAAGGAGCAATACGCTAATATCATCACGACCAATAACGAGTTATTACTAAAGTTAGTCAATGACATATTGGATTTG
GCAAGGATTGAATCCGGAGGTATCGTCTTTCATAAAGAATCCTGCAATTTAACGGATTTAGTGAAAACCCTTTATAAACA
ACATCTTTCCGATGTCCCCGAAGGTATCGAATTCAAGGAGAAATGCCCCGATACGCCGATCTGCTTATATACGGATAAGG
AGCGACTTTACCAAGCGCTCGAGAACATACTGAAAAATGCTATAAAATTTACTTCCGCAGGCTTTATAGAGATCGGTTAC
GAGTATTCCACCAATGCACAAGACGTCCGACTTTATGTGCAGGATACAGGCATCGGGATCTCTCCCGAGGACCAAGAAGC
GATATTCGAGCGTTTCAAGAAGCTGGATGATTTCGTGCAAGGGACAGGTCTTGGATTATCGATCTGCCAAGCTATCGTAA
AACAATTAAACGGGCGTATCTTGCTCAAGTCCGAAAAGAATAAGGGGTGCCGCATCTCCTTGGTTTTCGAATTATAA

Upstream 100 bases:

>100_bases
AAAAAAGCCATTGATATCCAATAATTATTAATAAGTTTGCACTCGCATAAAAACACTCATTTTAAATTACGATTAAGGGC
AACGTACTAATTTTCGACTT

Downstream 100 bases:

>100_bases
ACCAACCTCAGAAGCCAAGAAAAATAGACATTTGTCTCTTTGATATCCAATTACTTTTTATACATTTGTACCTATTATAT
TGAAGCTATACACATGGCGA

Product: putative two-component regulator sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 638; Mature: 638

Protein sequence:

>638_residues
MLARSIQKYYIFVLLFLLAILQSSCQQSENKYRILVVHSYESDYVAYKDCDRLIRKSLEKKGINPSIQTFYLNCEQYAAP
AEEKRMYLYLDSISTWKPDLILVYEDQATYTLMQCHHPLISTIPIVFGGVNFPNKALLAQYSNVSGFWDEPDYVTNIRLI
EHLLGKSTIYMLHDSTYIDRHIKATLHEQCAQADIRVDNNRIMYIPVEIATLDRVNQSLKRPDSTTVNVVPVQGDKLSAV
SWYMSKHVPYIYYLQAKRDYRVLNTSRFSSKPSFTAINEDLGYTNKLVGGYITSLETQIEESTDRAAEILKGSPSESFPQ
ITKSKKNYVFDFNEVKYWNIDKRLLPKDAILLNFPFKDQYPRLFWSLIIVVSTMCCFVFGSFIIMYTQESKAKRQAQKAL
LHEKESLAEALEKANESDRMKSVFLANMSHEIRTPLNAIIGFSSLIAELDLTAEEKEQYANIITTNNELLLKLVNDILDL
ARIESGGIVFHKESCNLTDLVKTLYKQHLSDVPEGIEFKEKCPDTPICLYTDKERLYQALENILKNAIKFTSAGFIEIGY
EYSTNAQDVRLYVQDTGIGISPEDQEAIFERFKKLDDFVQGTGLGLSICQAIVKQLNGRILLKSEKNKGCRISLVFEL

Sequences:

>Translated_638_residues
MLARSIQKYYIFVLLFLLAILQSSCQQSENKYRILVVHSYESDYVAYKDCDRLIRKSLEKKGINPSIQTFYLNCEQYAAP
AEEKRMYLYLDSISTWKPDLILVYEDQATYTLMQCHHPLISTIPIVFGGVNFPNKALLAQYSNVSGFWDEPDYVTNIRLI
EHLLGKSTIYMLHDSTYIDRHIKATLHEQCAQADIRVDNNRIMYIPVEIATLDRVNQSLKRPDSTTVNVVPVQGDKLSAV
SWYMSKHVPYIYYLQAKRDYRVLNTSRFSSKPSFTAINEDLGYTNKLVGGYITSLETQIEESTDRAAEILKGSPSESFPQ
ITKSKKNYVFDFNEVKYWNIDKRLLPKDAILLNFPFKDQYPRLFWSLIIVVSTMCCFVFGSFIIMYTQESKAKRQAQKAL
LHEKESLAEALEKANESDRMKSVFLANMSHEIRTPLNAIIGFSSLIAELDLTAEEKEQYANIITTNNELLLKLVNDILDL
ARIESGGIVFHKESCNLTDLVKTLYKQHLSDVPEGIEFKEKCPDTPICLYTDKERLYQALENILKNAIKFTSAGFIEIGY
EYSTNAQDVRLYVQDTGIGISPEDQEAIFERFKKLDDFVQGTGLGLSICQAIVKQLNGRILLKSEKNKGCRISLVFEL
>Mature_638_residues
MLARSIQKYYIFVLLFLLAILQSSCQQSENKYRILVVHSYESDYVAYKDCDRLIRKSLEKKGINPSIQTFYLNCEQYAAP
AEEKRMYLYLDSISTWKPDLILVYEDQATYTLMQCHHPLISTIPIVFGGVNFPNKALLAQYSNVSGFWDEPDYVTNIRLI
EHLLGKSTIYMLHDSTYIDRHIKATLHEQCAQADIRVDNNRIMYIPVEIATLDRVNQSLKRPDSTTVNVVPVQGDKLSAV
SWYMSKHVPYIYYLQAKRDYRVLNTSRFSSKPSFTAINEDLGYTNKLVGGYITSLETQIEESTDRAAEILKGSPSESFPQ
ITKSKKNYVFDFNEVKYWNIDKRLLPKDAILLNFPFKDQYPRLFWSLIIVVSTMCCFVFGSFIIMYTQESKAKRQAQKAL
LHEKESLAEALEKANESDRMKSVFLANMSHEIRTPLNAIIGFSSLIAELDLTAEEKEQYANIITTNNELLLKLVNDILDL
ARIESGGIVFHKESCNLTDLVKTLYKQHLSDVPEGIEFKEKCPDTPICLYTDKERLYQALENILKNAIKFTSAGFIEIGY
EYSTNAQDVRLYVQDTGIGISPEDQEAIFERFKKLDDFVQGTGLGLSICQAIVKQLNGRILLKSEKNKGCRISLVFEL

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=231, Percent_Identity=37.2294372294372, Blast_Score=139, Evalue=7e-34,
Organism=Escherichia coli, GI145693157, Length=242, Percent_Identity=32.6446280991736, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI48994928, Length=241, Percent_Identity=31.1203319502075, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI1788713, Length=246, Percent_Identity=30.0813008130081, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI87081816, Length=240, Percent_Identity=33.75, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1786912, Length=232, Percent_Identity=30.1724137931034, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788393, Length=249, Percent_Identity=29.3172690763052, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1786600, Length=235, Percent_Identity=25.531914893617, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790346, Length=228, Percent_Identity=27.6315789473684, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790436, Length=210, Percent_Identity=28.0952380952381, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1786783, Length=239, Percent_Identity=28.4518828451883, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790861, Length=298, Percent_Identity=26.8456375838926, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788549, Length=293, Percent_Identity=24.9146757679181, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87082128, Length=222, Percent_Identity=29.2792792792793, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788279, Length=232, Percent_Identity=30.6034482758621, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 73231; Mature: 73231

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLARSIQKYYIFVLLFLLAILQSSCQQSENKYRILVVHSYESDYVAYKDCDRLIRKSLEK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEHHHHHHHHHHHHHHH
KGINPSIQTFYLNCEQYAAPAEEKRMYLYLDSISTWKPDLILVYEDQATYTLMQCHHPLI
CCCCCCEEEEEEEHHHHCCCHHHCEEEEEEECCCCCCCCEEEEEECCCCEEHHHHCCHHH
STIPIVFGGVNFPNKALLAQYSNVSGFWDEPDYVTNIRLIEHLLGKSTIYMLHDSTYIDR
HHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHH
HIKATLHEQCAQADIRVDNNRIMYIPVEIATLDRVNQSLKRPDSTTVNVVPVQGDKLSAV
HHHHHHHHHHHCCCEEECCCEEEEEEEEEHHHHHHHHHHCCCCCCEEEEEEECCCCCHHH
SWYMSKHVPYIYYLQAKRDYRVLNTSRFSSKPSFTAINEDLGYTNKLVGGYITSLETQIE
HHHHHCCCCEEEEEEECCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHH
ESTDRAAEILKGSPSESFPQITKSKKNYVFDFNEVKYWNIDKRLLPKDAILLNFPFKDQY
HHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEECCEEEECCCHHHCCCCEEEEECCCCCCH
PRLFWSLIIVVSTMCCFVFGSFIIMYTQESKAKRQAQKALLHEKESLAEALEKANESDRM
HHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KSVFLANMSHEIRTPLNAIIGFSSLIAELDLTAEEKEQYANIITTNNELLLKLVNDILDL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEECCHHHHHHHHHHHHHH
ARIESGGIVFHKESCNLTDLVKTLYKQHLSDVPEGIEFKEKCPDTPICLYTDKERLYQAL
HHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEECCHHHHHHHH
ENILKNAIKFTSAGFIEIGYEYSTNAQDVRLYVQDTGIGISPEDQEAIFERFKKLDDFVQ
HHHHHHHHHHCCCCEEEECCEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
GTGLGLSICQAIVKQLNGRILLKSEKNKGCRISLVFEL
CCCCCHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEEC
>Mature Secondary Structure
MLARSIQKYYIFVLLFLLAILQSSCQQSENKYRILVVHSYESDYVAYKDCDRLIRKSLEK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEHHHHHHHHHHHHHHH
KGINPSIQTFYLNCEQYAAPAEEKRMYLYLDSISTWKPDLILVYEDQATYTLMQCHHPLI
CCCCCCEEEEEEEHHHHCCCHHHCEEEEEEECCCCCCCCEEEEEECCCCEEHHHHCCHHH
STIPIVFGGVNFPNKALLAQYSNVSGFWDEPDYVTNIRLIEHLLGKSTIYMLHDSTYIDR
HHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHH
HIKATLHEQCAQADIRVDNNRIMYIPVEIATLDRVNQSLKRPDSTTVNVVPVQGDKLSAV
HHHHHHHHHHHCCCEEECCCEEEEEEEEEHHHHHHHHHHCCCCCCEEEEEEECCCCCHHH
SWYMSKHVPYIYYLQAKRDYRVLNTSRFSSKPSFTAINEDLGYTNKLVGGYITSLETQIE
HHHHHCCCCEEEEEEECCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHH
ESTDRAAEILKGSPSESFPQITKSKKNYVFDFNEVKYWNIDKRLLPKDAILLNFPFKDQY
HHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEECCEEEECCCHHHCCCCEEEEECCCCCCH
PRLFWSLIIVVSTMCCFVFGSFIIMYTQESKAKRQAQKALLHEKESLAEALEKANESDRM
HHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KSVFLANMSHEIRTPLNAIIGFSSLIAELDLTAEEKEQYANIITTNNELLLKLVNDILDL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEECCHHHHHHHHHHHHHH
ARIESGGIVFHKESCNLTDLVKTLYKQHLSDVPEGIEFKEKCPDTPICLYTDKERLYQAL
HHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEECCHHHHHHHH
ENILKNAIKFTSAGFIEIGYEYSTNAQDVRLYVQDTGIGISPEDQEAIFERFKKLDDFVQ
HHHHHHHHHHCCCCEEEECCEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
GTGLGLSICQAIVKQLNGRILLKSEKNKGCRISLVFEL
CCCCCHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]