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The map label for this gene is rng [H]

Identifier: 150008610

GI number: 150008610

Start: 2358724

End: 2360301

Strand: Direct

Name: rng [H]

Synonym: BDI_1996

Alternate gene names: 150008610

Gene position: 2358724-2360301 (Clockwise)

Preceding gene: 150008609

Following gene: 150008611

Centisome position: 49.02

GC content: 45.31

Gene sequence:

>1578_bases
GTGATTAGTGAATTAGTAGTTGATGTACAGCCCAAAGAGGTATCTATCGCCGTCTTGGAGGACAAGAGCCTTGTGGAGCT
CCAAAAGGAGGCCCGCAACGTCTCGTTCGCTGTTGGTGATATCTATCTAGGTAAGGTTAAGAAACTGATGCCGGGCTTAA
ACGCTGCGTTCATTGATGTCGGTTACAAAAAGGATGCGTTTCTTCACTATTTGGACTTAGGTCCTAACTTCAACACCCAA
CAGAAATATTTAAAGCAATTGCTGAGCGATCCCAAAAAGGCGCCGGTACTGTCGAAAACTCAGATTCTACCGGAGATCGA
AAAGAACGGGAGTATTAGCGACGTGCTGAAAGTAGGACAAGAGGTGTTGGTTCAAATTGCTAAAGAGCCTATCTCGACGA
AAGGTCCCCGATTGACTTCCGAGCTTTCTTTTGCCGGAAGATATATCGTGCTGATACCGTTCGCCGATAAAGTATCTGTC
TCCACCAAAATTAAATCAAGCGAGGAACGAGCCCGTTTGCGCCAGTTAATCCAAAGTATCAAACCGAAGAATTTCAGTGT
TATCGTACGGACATCCTCGGAGGGCAAGCGTGTCGCAGAGCTTGATCATGAATTGAAGACATTGATGAAACGATGGGAAG
ATAATATCGTGAAGGTGCCTAAACTCAAAGCTCCGGCTATTATTTATGAGGAAACGGCACGTACCGTAGCTTTGCTTCGG
GATATATTCAACCCCTCTTTCCAAAATATTTATGTAAATGACAAGGAGGTCTATAATAATGTCCGCGACTATGTAAGCTT
GATCGCACCGGGACGGGAAGAAATCGTACAGTTATATACGGGAGAGCTTCCTATCTTCGACAACTTTGCGGTCACCAAGC
AAATCAAGTCGCTATTCGGACGAACTGTCACTTACAAGAGTGGAGCTTACTTGATTATCGAGCACACGGAAGCTATGCAC
GTTATCGACGTGAACAGCGGGAACCGTTCCAAAGGCAGCGATGCCCAAGAGAAGACAGCTATCGACGTAAATATAGCCGC
CGCCGATGAGATCGCCCGTCAGCTCCGCTTACGAGACATGGGAGGTATCATCGTCATAGACTTTATCGATATGGCAGAGG
CTGCCAACCGGCAAAAGCTATTTGAACACATGACAAAGGCAATGGCAAATGACCGGGCTAAACACAATATCCTTCCTCTT
AGTAAGTTTGGCTTGATGCAGATTACCCGTCAGCGGGTACGTCCGGCAATGGACGTGGATACGTCAGAAGACTGCCCATC
CTGTTTCGGAACAGGAACGGTTAAGCCTTCCATCCTGTTTACCGACAGCCTGGAAGAAAAAATAGATTGCTTAGTAAACA
AACATCACGTGAAGAAATTCACCCTGCATGTACATCCGTATGTCGCAGCGTATGTGAACAAGGGGTTGTTCCCCTTGAGC
ATGAAGTGGAAAATGAAGTACACACATGGTTTAAAAGTTATTCCCAATCAAAGTCTCGCCTTTCTTGAATACAAATTCTT
TGATGCCGACAAGAATGAGCTTGACATGAAAGAAGAAAAGGAAATAATCAACAAATAA

Upstream 100 bases:

>100_bases
AAGTCTATCTAACGAACTCTAAAGAACAAACTTAATAAGTGACCTTTTAAGTTTGTTCTTTGCGTATTTTATAAGGTCTG
ATCTTAAAAACACATCTTAA

Downstream 100 bases:

>100_bases
CAAGAGAAGCCCCGGGCCTATTAAAACGGTTCCGGGGCTTTCTGTATTCTGCCAAACCGCCAAAATGTCACGATTCATGA
CACGTATTATTTATTTTCAA

Product: ribonuclease G

Products: Cleaved RNA [C]

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MISELVVDVQPKEVSIAVLEDKSLVELQKEARNVSFAVGDIYLGKVKKLMPGLNAAFIDVGYKKDAFLHYLDLGPNFNTQ
QKYLKQLLSDPKKAPVLSKTQILPEIEKNGSISDVLKVGQEVLVQIAKEPISTKGPRLTSELSFAGRYIVLIPFADKVSV
STKIKSSEERARLRQLIQSIKPKNFSVIVRTSSEGKRVAELDHELKTLMKRWEDNIVKVPKLKAPAIIYEETARTVALLR
DIFNPSFQNIYVNDKEVYNNVRDYVSLIAPGREEIVQLYTGELPIFDNFAVTKQIKSLFGRTVTYKSGAYLIIEHTEAMH
VIDVNSGNRSKGSDAQEKTAIDVNIAAADEIARQLRLRDMGGIIVIDFIDMAEAANRQKLFEHMTKAMANDRAKHNILPL
SKFGLMQITRQRVRPAMDVDTSEDCPSCFGTGTVKPSILFTDSLEEKIDCLVNKHHVKKFTLHVHPYVAAYVNKGLFPLS
MKWKMKYTHGLKVIPNQSLAFLEYKFFDADKNELDMKEEKEIINK

Sequences:

>Translated_525_residues
MISELVVDVQPKEVSIAVLEDKSLVELQKEARNVSFAVGDIYLGKVKKLMPGLNAAFIDVGYKKDAFLHYLDLGPNFNTQ
QKYLKQLLSDPKKAPVLSKTQILPEIEKNGSISDVLKVGQEVLVQIAKEPISTKGPRLTSELSFAGRYIVLIPFADKVSV
STKIKSSEERARLRQLIQSIKPKNFSVIVRTSSEGKRVAELDHELKTLMKRWEDNIVKVPKLKAPAIIYEETARTVALLR
DIFNPSFQNIYVNDKEVYNNVRDYVSLIAPGREEIVQLYTGELPIFDNFAVTKQIKSLFGRTVTYKSGAYLIIEHTEAMH
VIDVNSGNRSKGSDAQEKTAIDVNIAAADEIARQLRLRDMGGIIVIDFIDMAEAANRQKLFEHMTKAMANDRAKHNILPL
SKFGLMQITRQRVRPAMDVDTSEDCPSCFGTGTVKPSILFTDSLEEKIDCLVNKHHVKKFTLHVHPYVAAYVNKGLFPLS
MKWKMKYTHGLKVIPNQSLAFLEYKFFDADKNELDMKEEKEIINK
>Mature_525_residues
MISELVVDVQPKEVSIAVLEDKSLVELQKEARNVSFAVGDIYLGKVKKLMPGLNAAFIDVGYKKDAFLHYLDLGPNFNTQ
QKYLKQLLSDPKKAPVLSKTQILPEIEKNGSISDVLKVGQEVLVQIAKEPISTKGPRLTSELSFAGRYIVLIPFADKVSV
STKIKSSEERARLRQLIQSIKPKNFSVIVRTSSEGKRVAELDHELKTLMKRWEDNIVKVPKLKAPAIIYEETARTVALLR
DIFNPSFQNIYVNDKEVYNNVRDYVSLIAPGREEIVQLYTGELPIFDNFAVTKQIKSLFGRTVTYKSGAYLIIEHTEAMH
VIDVNSGNRSKGSDAQEKTAIDVNIAAADEIARQLRLRDMGGIIVIDFIDMAEAANRQKLFEHMTKAMANDRAKHNILPL
SKFGLMQITRQRVRPAMDVDTSEDCPSCFGTGTVKPSILFTDSLEEKIDCLVNKHHVKKFTLHVHPYVAAYVNKGLFPLS
MKWKMKYTHGLKVIPNQSLAFLEYKFFDADKNELDMKEEKEIINK

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=466, Percent_Identity=33.0472103004292, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI87082235, Length=472, Percent_Identity=33.4745762711864, Blast_Score=249, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 59362; Mature: 59362

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISELVVDVQPKEVSIAVLEDKSLVELQKEARNVSFAVGDIYLGKVKKLMPGLNAAFIDV
CCCCEEEECCCCEEEEEEECCCHHHHHHHHHCCCEEEECHHHHHHHHHHCCCCCEEEEEC
GYKKDAFLHYLDLGPNFNTQQKYLKQLLSDPKKAPVLSKTQILPEIEKNGSISDVLKVGQ
CCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCHHHCCCCHHHHHHHHH
EVLVQIAKEPISTKGPRLTSELSFAGRYIVLIPFADKVSVSTKIKSSEERARLRQLIQSI
HHHHHHHHCCCCCCCCCCHHHHHHCCCEEEEEECCCCCEEEHHCCCHHHHHHHHHHHHHC
KPKNFSVIVRTSSEGKRVAELDHELKTLMKRWEDNIVKVPKLKAPAIIYEETARTVALLR
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEHHHHHHHHHHH
DIFNPSFQNIYVNDKEVYNNVRDYVSLIAPGREEIVQLYTGELPIFDNFAVTKQIKSLFG
HHCCCCCCEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
RTVTYKSGAYLIIEHTEAMHVIDVNSGNRSKGSDAQEKTAIDVNIAAADEIARQLRLRDM
CEEEECCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHHHCC
GGIIVIDFIDMAEAANRQKLFEHMTKAMANDRAKHNILPLSKFGLMQITRQRVRPAMDVD
CCEEEEEEHHHHHHCCHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCC
TSEDCPSCFGTGTVKPSILFTDSLEEKIDCLVNKHHVKKFTLHVHPYVAAYVNKGLFPLS
CCCCCCHHCCCCCCCCEEEEECCHHHHHHHHHCHHCEEEEEEEECHHHHHHHCCCCCEEE
MKWKMKYTHGLKVIPNQSLAFLEYKFFDADKNELDMKEEKEIINK
EEEEEEECCCEEECCCCCEEEEEEEEECCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MISELVVDVQPKEVSIAVLEDKSLVELQKEARNVSFAVGDIYLGKVKKLMPGLNAAFIDV
CCCCEEEECCCCEEEEEEECCCHHHHHHHHHCCCEEEECHHHHHHHHHHCCCCCEEEEEC
GYKKDAFLHYLDLGPNFNTQQKYLKQLLSDPKKAPVLSKTQILPEIEKNGSISDVLKVGQ
CCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCHHHCCCCHHHHHHHHH
EVLVQIAKEPISTKGPRLTSELSFAGRYIVLIPFADKVSVSTKIKSSEERARLRQLIQSI
HHHHHHHHCCCCCCCCCCHHHHHHCCCEEEEEECCCCCEEEHHCCCHHHHHHHHHHHHHC
KPKNFSVIVRTSSEGKRVAELDHELKTLMKRWEDNIVKVPKLKAPAIIYEETARTVALLR
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEHHHHHHHHHHH
DIFNPSFQNIYVNDKEVYNNVRDYVSLIAPGREEIVQLYTGELPIFDNFAVTKQIKSLFG
HHCCCCCCEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
RTVTYKSGAYLIIEHTEAMHVIDVNSGNRSKGSDAQEKTAIDVNIAAADEIARQLRLRDM
CEEEECCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHHHCC
GGIIVIDFIDMAEAANRQKLFEHMTKAMANDRAKHNILPLSKFGLMQITRQRVRPAMDVD
CCEEEEEEHHHHHHCCHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCC
TSEDCPSCFGTGTVKPSILFTDSLEEKIDCLVNKHHVKKFTLHVHPYVAAYVNKGLFPLS
CCCCCCHHCCCCCCCCEEEEECCHHHHHHHHHCHHCEEEEEEEECHHHHHHHCCCCCEEE
MKWKMKYTHGLKVIPNQSLAFLEYKFFDADKNELDMKEEKEIINK
EEEEEEECCCEEECCCCCEEEEEEEEECCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RNA [C]

Specific reaction: Cleaves RNA endonucleolytically in AU-rich single-strand regions [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]