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The map label for this gene is ybiW [H]

Identifier: 150008479

GI number: 150008479

Start: 2217286

End: 2219565

Strand: Direct

Name: ybiW [H]

Synonym: BDI_1865

Alternate gene names: 150008479

Gene position: 2217286-2219565 (Clockwise)

Preceding gene: 150008478

Following gene: 150008480

Centisome position: 46.08

GC content: 46.75

Gene sequence:

>2280_bases
ATGGCAAATTTCAATGTAGAAGGGGCGCAAAACGTAGAGATCAGCAAGAAATTACCTTGCGAGAAACCTCTGATCGTACA
ACTGGAGATCATGGAGCGGTATACGGCAGTCCATAAAGCCTGTGCCGCCCTACCCAAAGAGATACGTGAAGTGGAATGCC
TGAAAGTTTTATACCCCACGCTATTTCGCAAGATCACGAATCAAGACTTGATCGCCGGACGTACGGACTTCCTACCGATC
GGGTTCGGATGCGTAACCTCATTAGGGGGTGTCGGGCATTATTGCGTATTCAAGAAACTCCGTGCCTTCCAGCTACAACT
GGATGAAACTGAACGAAAACGGGTGGATGCGTTATATGATTATTGGTTGGATCACGATTTAAAGACCCAATTCAACAAGG
AGGTCCTCACCGAGGATACATTGGGTATGTTCATCGACTGCGAATACCCGATGATCGCTACCGCACGTCTATCTGGAATG
ATGCTGGATTACCCGAAACTATTGGATAAGGGCATCGGTGGCCTTCGCTCTGATTTACAGGAGAAACTCAAAGAGCAACC
CGATAATAACTTTTACAAAGCAGGCATCCAATGTCTGGATATTTTTGTAGATTGTGCTTCTCATCTCCAGCAAGACGCTA
GAGAGCAAATGGCTTCAGCCAACATGAAAAGGCAAAAAGAATTAGAGCGTATCTGCCAAGCCCTAGAGAACATCAAGGAG
AAGAAACCCGGTACGTTCCATGAAGCAATGCAGTTATTCTGGTTATTCGCTTTGTTGGCCGGTGTTATAAACTACGGTCG
TCTGGATGATTATCTAGGCCCTTATTTAGTAGCCGATCTCAAGTCCGGGCGATTGACCGACGAAGAGGCATACCGATATA
TCCACTCGCTTTGGACCATGATTGAGAATCGCCGTACCACCGTAAACGGACGTATCATCGTAGGCGGTAAAGGGCGCAAA
CACCCGAAAGAGGCCGACGTATTCCTGCATATCGCCATGAAAGTAGCGAAAAATTGCAGATATGTAGAGCCACAATTCAC
CTTGCGCTTCGATAAAGAGACCTCCGAGGAAATCTGGGACGAAGCGTTGGACGCTTTGGGTGCGGGAGCTACTTACCCGA
CCCTTTACAATGATGATGTAAACGTCCCGGCCGTCATGTACGGAATGCGTGTAGACGAGAAAACGGCGGAACAATACGTG
CCTTTCGGTTGTACCGAGTTCGTGATACAAGGACAGAGTACGGGTACGCCCAACATTTGCATCAATTTACTAAAACTACT
TACGATTTATATGAATGATGGTATCGACCCGATTGACGGTAAACGTAAATCCGGGCCGGTATCTCTAAAGAAATTAGAGG
AGTATCAGACATTCGAGGAATTCTATGACGGTTACAAGGCTTTACTCGATTATTATCTGGATCTGTCTGTCAAAGCCCAA
TACCATTCGTATGAGGTGATGAACCAGCACGTAAGTTTCTTGTTTACCAGCCTATTGACCGATGATTGCATCGCACGGGG
AAAAGCGCTTTTGGACGGAGGCGTACGTTATCTAGGCGGGACAAACGAGACGTATGGCAATATCAACACTTCCGATTCCC
TGTGGGTAATCCGGGATCTGGTATTCAATCAAAAAAAATACACGTTACGGCAATTGAATGATGCCATGCTAGCTAATTTC
AACGGCTATGAGGCTTTACGAAAAGATTGCCTGAACTGTGATAAATATGGTAACGACCTTGAGACTGCCGATACGATGGC
CAACGATTTGTACGAGTTCGTAGCGAAAGGCGTACGCGATCGCGGTATCGCCATCGGTATGCAATACTTCTTGATCGTTA
TCAGCAATAACCAATTAAACACGGAATGGGGAAACCGGACCGCCGCCTCACCCGACGGACGATTATCGTGCATGTATATG
AATCCTGCCAATAATCCGCAAGGGGGAGCGAATAAAAGCGGCCCCACGGCCATGCTGAATTCCTTGGCGAAGTTCGACGC
CAAGTATCATGGAGGCTCGGTACAAAACATTAAGTTTTCTCCTTCCATGTTCAACGAGAACAGGGCTGTCATCAAGAGCC
TGTTCAAGACTTATTTTGCGAAAGGGGGTTGCCATTTGATGGTGACTGTAGTAGATAAGGGAGTTTTAGAAGACGCGGTA
GAGCACCCGGAAAATTACCCGGACCTAATCGTCCGGGTAAGCGGGTTCTCCGCGGTATTCGTCGATTTATCTCCCAATAT
TCAACAGGAACTTTTAAGCAGGGTATTGTATGATAAATAA

Upstream 100 bases:

>100_bases
ATCACCCGTCCGCACTTGATCACGAAACGTTTTACGGACTTACTAACCGGTTACCAAAATAACTGGCGGGAAGCCGAAAG
AAGCAAACATTAATAGTCAT

Downstream 100 bases:

>100_bases
ATATAAGGTTAGCGAGAATAGGTTATCAATCATGGAGATCGAACGGTTCGCCGTTCACGACGGCCCGGGCATCCGGACCG
TCGTTTTCCTTCAAGGTTGT

Product: putative formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 3 [H]

Number of amino acids: Translated: 759; Mature: 758

Protein sequence:

>759_residues
MANFNVEGAQNVEISKKLPCEKPLIVQLEIMERYTAVHKACAALPKEIREVECLKVLYPTLFRKITNQDLIAGRTDFLPI
GFGCVTSLGGVGHYCVFKKLRAFQLQLDETERKRVDALYDYWLDHDLKTQFNKEVLTEDTLGMFIDCEYPMIATARLSGM
MLDYPKLLDKGIGGLRSDLQEKLKEQPDNNFYKAGIQCLDIFVDCASHLQQDAREQMASANMKRQKELERICQALENIKE
KKPGTFHEAMQLFWLFALLAGVINYGRLDDYLGPYLVADLKSGRLTDEEAYRYIHSLWTMIENRRTTVNGRIIVGGKGRK
HPKEADVFLHIAMKVAKNCRYVEPQFTLRFDKETSEEIWDEALDALGAGATYPTLYNDDVNVPAVMYGMRVDEKTAEQYV
PFGCTEFVIQGQSTGTPNICINLLKLLTIYMNDGIDPIDGKRKSGPVSLKKLEEYQTFEEFYDGYKALLDYYLDLSVKAQ
YHSYEVMNQHVSFLFTSLLTDDCIARGKALLDGGVRYLGGTNETYGNINTSDSLWVIRDLVFNQKKYTLRQLNDAMLANF
NGYEALRKDCLNCDKYGNDLETADTMANDLYEFVAKGVRDRGIAIGMQYFLIVISNNQLNTEWGNRTAASPDGRLSCMYM
NPANNPQGGANKSGPTAMLNSLAKFDAKYHGGSVQNIKFSPSMFNENRAVIKSLFKTYFAKGGCHLMVTVVDKGVLEDAV
EHPENYPDLIVRVSGFSAVFVDLSPNIQQELLSRVLYDK

Sequences:

>Translated_759_residues
MANFNVEGAQNVEISKKLPCEKPLIVQLEIMERYTAVHKACAALPKEIREVECLKVLYPTLFRKITNQDLIAGRTDFLPI
GFGCVTSLGGVGHYCVFKKLRAFQLQLDETERKRVDALYDYWLDHDLKTQFNKEVLTEDTLGMFIDCEYPMIATARLSGM
MLDYPKLLDKGIGGLRSDLQEKLKEQPDNNFYKAGIQCLDIFVDCASHLQQDAREQMASANMKRQKELERICQALENIKE
KKPGTFHEAMQLFWLFALLAGVINYGRLDDYLGPYLVADLKSGRLTDEEAYRYIHSLWTMIENRRTTVNGRIIVGGKGRK
HPKEADVFLHIAMKVAKNCRYVEPQFTLRFDKETSEEIWDEALDALGAGATYPTLYNDDVNVPAVMYGMRVDEKTAEQYV
PFGCTEFVIQGQSTGTPNICINLLKLLTIYMNDGIDPIDGKRKSGPVSLKKLEEYQTFEEFYDGYKALLDYYLDLSVKAQ
YHSYEVMNQHVSFLFTSLLTDDCIARGKALLDGGVRYLGGTNETYGNINTSDSLWVIRDLVFNQKKYTLRQLNDAMLANF
NGYEALRKDCLNCDKYGNDLETADTMANDLYEFVAKGVRDRGIAIGMQYFLIVISNNQLNTEWGNRTAASPDGRLSCMYM
NPANNPQGGANKSGPTAMLNSLAKFDAKYHGGSVQNIKFSPSMFNENRAVIKSLFKTYFAKGGCHLMVTVVDKGVLEDAV
EHPENYPDLIVRVSGFSAVFVDLSPNIQQELLSRVLYDK
>Mature_758_residues
ANFNVEGAQNVEISKKLPCEKPLIVQLEIMERYTAVHKACAALPKEIREVECLKVLYPTLFRKITNQDLIAGRTDFLPIG
FGCVTSLGGVGHYCVFKKLRAFQLQLDETERKRVDALYDYWLDHDLKTQFNKEVLTEDTLGMFIDCEYPMIATARLSGMM
LDYPKLLDKGIGGLRSDLQEKLKEQPDNNFYKAGIQCLDIFVDCASHLQQDAREQMASANMKRQKELERICQALENIKEK
KPGTFHEAMQLFWLFALLAGVINYGRLDDYLGPYLVADLKSGRLTDEEAYRYIHSLWTMIENRRTTVNGRIIVGGKGRKH
PKEADVFLHIAMKVAKNCRYVEPQFTLRFDKETSEEIWDEALDALGAGATYPTLYNDDVNVPAVMYGMRVDEKTAEQYVP
FGCTEFVIQGQSTGTPNICINLLKLLTIYMNDGIDPIDGKRKSGPVSLKKLEEYQTFEEFYDGYKALLDYYLDLSVKAQY
HSYEVMNQHVSFLFTSLLTDDCIARGKALLDGGVRYLGGTNETYGNINTSDSLWVIRDLVFNQKKYTLRQLNDAMLANFN
GYEALRKDCLNCDKYGNDLETADTMANDLYEFVAKGVRDRGIAIGMQYFLIVISNNQLNTEWGNRTAASPDGRLSCMYMN
PANNPQGGANKSGPTAMLNSLAKFDAKYHGGSVQNIKFSPSMFNENRAVIKSLFKTYFAKGGCHLMVTVVDKGVLEDAVE
HPENYPDLIVRVSGFSAVFVDLSPNIQQELLSRVLYDK

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787044, Length=695, Percent_Identity=26.1870503597122, Blast_Score=231, Evalue=2e-61,
Organism=Escherichia coli, GI1790388, Length=774, Percent_Identity=25.4521963824289, Blast_Score=215, Evalue=8e-57,
Organism=Escherichia coli, GI48994926, Length=604, Percent_Identity=25.9933774834437, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1787131, Length=555, Percent_Identity=24.8648648648649, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 85964; Mature: 85833

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANFNVEGAQNVEISKKLPCEKPLIVQLEIMERYTAVHKACAALPKEIREVECLKVLYPT
CCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFRKITNQDLIAGRTDFLPIGFGCVTSLGGVGHYCVFKKLRAFQLQLDETERKRVDALYD
HHHHHCCCCHHCCCCCCCEECHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
YWLDHDLKTQFNKEVLTEDTLGMFIDCEYPMIATARLSGMMLDYPKLLDKGIGGLRSDLQ
HHHCCCHHHHHHHHHHHHCCCCEEEECCCCEEHHHHHHCHHHHHHHHHHHCCCHHHHHHH
EKLKEQPDNNFYKAGIQCLDIFVDCASHLQQDAREQMASANMKRQKELERICQALENIKE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KKPGTFHEAMQLFWLFALLAGVINYGRLDDYLGPYLVADLKSGRLTDEEAYRYIHSLWTM
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEHHCCCCCCCHHHHHHHHHHHHHH
IENRRTTVNGRIIVGGKGRKHPKEADVFLHIAMKVAKNCRYVEPQFTLRFDKETSEEIWD
HHCCCEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHH
EALDALGAGATYPTLYNDDVNVPAVMYGMRVDEKTAEQYVPFGCTEFVIQGQSTGTPNIC
HHHHHHCCCCCCCCEECCCCCCCHHEECCCCCHHHHHHCCCCCCHHHEEECCCCCCHHHH
INLLKLLTIYMNDGIDPIDGKRKSGPVSLKKLEEYQTFEEFYDGYKALLDYYLDLSVKAQ
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEH
YHSYEVMNQHVSFLFTSLLTDDCIARGKALLDGGVRYLGGTNETYGNINTSDSLWVIRDL
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
VFNQKKYTLRQLNDAMLANFNGYEALRKDCLNCDKYGNDLETADTMANDLYEFVAKGVRD
HHCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHCHHH
RGIAIGMQYFLIVISNNQLNTEWGNRTAASPDGRLSCMYMNPANNPQGGANKSGPTAMLN
CCCEEEEEEEEEEEECCEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHH
SLAKFDAKYHGGSVQNIKFSPSMFNENRAVIKSLFKTYFAKGGCHLMVTVVDKGVLEDAV
HHHHHHHHHCCCCCCEEEECCCHHCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHH
EHPENYPDLIVRVSGFSAVFVDLSPNIQQELLSRVLYDK
HCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
ANFNVEGAQNVEISKKLPCEKPLIVQLEIMERYTAVHKACAALPKEIREVECLKVLYPT
CCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFRKITNQDLIAGRTDFLPIGFGCVTSLGGVGHYCVFKKLRAFQLQLDETERKRVDALYD
HHHHHCCCCHHCCCCCCCEECHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
YWLDHDLKTQFNKEVLTEDTLGMFIDCEYPMIATARLSGMMLDYPKLLDKGIGGLRSDLQ
HHHCCCHHHHHHHHHHHHCCCCEEEECCCCEEHHHHHHCHHHHHHHHHHHCCCHHHHHHH
EKLKEQPDNNFYKAGIQCLDIFVDCASHLQQDAREQMASANMKRQKELERICQALENIKE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KKPGTFHEAMQLFWLFALLAGVINYGRLDDYLGPYLVADLKSGRLTDEEAYRYIHSLWTM
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEHHCCCCCCCHHHHHHHHHHHHHH
IENRRTTVNGRIIVGGKGRKHPKEADVFLHIAMKVAKNCRYVEPQFTLRFDKETSEEIWD
HHCCCEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHH
EALDALGAGATYPTLYNDDVNVPAVMYGMRVDEKTAEQYVPFGCTEFVIQGQSTGTPNIC
HHHHHHCCCCCCCCEECCCCCCCHHEECCCCCHHHHHHCCCCCCHHHEEECCCCCCHHHH
INLLKLLTIYMNDGIDPIDGKRKSGPVSLKKLEEYQTFEEFYDGYKALLDYYLDLSVKAQ
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEH
YHSYEVMNQHVSFLFTSLLTDDCIARGKALLDGGVRYLGGTNETYGNINTSDSLWVIRDL
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
VFNQKKYTLRQLNDAMLANFNGYEALRKDCLNCDKYGNDLETADTMANDLYEFVAKGVRD
HHCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHCHHH
RGIAIGMQYFLIVISNNQLNTEWGNRTAASPDGRLSCMYMNPANNPQGGANKSGPTAMLN
CCCEEEEEEEEEEEECCEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHH
SLAKFDAKYHGGSVQNIKFSPSMFNENRAVIKSLFKTYFAKGGCHLMVTVVDKGVLEDAV
HHHHHHHHHCCCCCCEEEECCCHHCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHH
EHPENYPDLIVRVSGFSAVFVDLSPNIQQELLSRVLYDK
HCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]