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The map label for this gene is ispD [H]

Identifier: 150008457

GI number: 150008457

Start: 2193354

End: 2194724

Strand: Reverse

Name: ispD [H]

Synonym: BDI_1841

Alternate gene names: 150008457

Gene position: 2194724-2193354 (Counterclockwise)

Preceding gene: 150008458

Following gene: 150008456

Centisome position: 45.62

GC content: 41.14

Gene sequence:

>1371_bases
ATGAGAAATATAGCTGTGGTTTTGGCCGGTGGTTTCGGTCAACGTTTAGGAGATGCGACACCTAAACAGTTCTTGAAGTT
AGCGGGGAAAAAAGTGATTGAACATACGATAGATGTTTTCCAAAATCATCCGTTAATAGATGAGATCGTTGTGGTTTCTA
ATCCTAATTATGTGCAGGAGGTAGAGAATATCTTGGTGAAGAATGAATATTCCAAGATGAAGAAAATCCTCAAGGGAGGA
AAGGAACGTTACCATTCAAGTTTAGCGGCGATTAATGCTTATGAGGAAGAGGTGAATATGATTTTCCACGATGCGGTTCG
CCCATTGGTCAATGATCGTATTATATCTGATTGTATCAATGCCTTGAAAACGTATAATGCGGTGGATGTCGCAATCAAGA
CGACTGATACGATCATTCAAATTGATGCGGCGGACCATATTACCGGAATACCTGCTAGGGAGTATTTGCGTAACGGACAG
ACTCCGCAAGCTTTTAAGCGTTCTACGATAAAACGAGCTTATGAATTGGCTTTAAAGGATGTTAGTTTTCAAACGACAGA
TGATTGTGGGGTTGTCTACAAGTATCTTCCGGAAGAATTCGTGTATGTAGTCAAGGGTGAACAGTTTAATATGAAATTGA
CTTACAAAGAAGATCTGTTTTTATTGGATAAATTGTTTCAGCTAAAATCAATCGCTCAACAGAATGAAACGGTAACTCCT
AAGACTATAGCCGCTTTGCCTTCATCGGTTATCGTTGTTTTTGGCGGAAGCTATGGGATTGGGCTTGATATTATCCATAT
TTGTCGCCAGTACGGGGCGCATATCTATTCTTTCAGCCGTTCAGAGAATGGAGTGGATGTCTCCGATCCTGCTCTAGTGG
CTGATGCTTTGAGAACTGTCATGGATAAGGAAGGACGTATAGATGCGGTTGTCAATACGGCTGGAGTCTTGAATCGGGAG
CCATTGGCTACAATGACATATGAGGAGATTTGTAAAAGTATTCATGTGAATTATCTTGGAGCTGTTATTGTAGCAAGGGA
ATCTTATCCGTATTTAAAAATGAGCAAAGGGGCGTTGCTGCTGTTTACTTCCAGTTCTTATACGAGGGGGCGTCAATTAT
ATAGCTTGTATTCATCTTCTAAAGCCGCTATCGTGAATTTTGTACAAGCGTTGAGTGAAGAGTGGCAGGATAGTGGTATT
CGTATCAATTGTATTAATCCGGAACGGACTAAGACCCCTATGCGGGAGAGAAATTTTGGCATAGAACCGGAGGATACCTT
GTTGAAATCAAGTGTTGTTGCGTCTGTGTCGGTGAATACTATATTTTCTTCCCTGACCGGAGAAGTGGTGGATGTTAAAA
AAAGACCGTAA

Upstream 100 bases:

>100_bases
GGTATATTGAGAAAGATGCGCTTCGTGATTTTTCTGCTTCTTTTGATAAAATACAAGCGACTCAAAGTTTAATATCTTAA
AGATTCATATTCTATACATC

Downstream 100 bases:

>100_bases
AGATGAAATCTGATAGTGAATTGATGAATGAGTTAGCTCATAAAACGAATTATTTACATGAGATGACATCTTCGGAGTCT
TCTGCCATGAAGCGAGCTTT

Product: pyrophosphorylase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MRNIAVVLAGGFGQRLGDATPKQFLKLAGKKVIEHTIDVFQNHPLIDEIVVVSNPNYVQEVENILVKNEYSKMKKILKGG
KERYHSSLAAINAYEEEVNMIFHDAVRPLVNDRIISDCINALKTYNAVDVAIKTTDTIIQIDAADHITGIPAREYLRNGQ
TPQAFKRSTIKRAYELALKDVSFQTTDDCGVVYKYLPEEFVYVVKGEQFNMKLTYKEDLFLLDKLFQLKSIAQQNETVTP
KTIAALPSSVIVVFGGSYGIGLDIIHICRQYGAHIYSFSRSENGVDVSDPALVADALRTVMDKEGRIDAVVNTAGVLNRE
PLATMTYEEICKSIHVNYLGAVIVARESYPYLKMSKGALLLFTSSSYTRGRQLYSLYSSSKAAIVNFVQALSEEWQDSGI
RINCINPERTKTPMRERNFGIEPEDTLLKSSVVASVSVNTIFSSLTGEVVDVKKRP

Sequences:

>Translated_456_residues
MRNIAVVLAGGFGQRLGDATPKQFLKLAGKKVIEHTIDVFQNHPLIDEIVVVSNPNYVQEVENILVKNEYSKMKKILKGG
KERYHSSLAAINAYEEEVNMIFHDAVRPLVNDRIISDCINALKTYNAVDVAIKTTDTIIQIDAADHITGIPAREYLRNGQ
TPQAFKRSTIKRAYELALKDVSFQTTDDCGVVYKYLPEEFVYVVKGEQFNMKLTYKEDLFLLDKLFQLKSIAQQNETVTP
KTIAALPSSVIVVFGGSYGIGLDIIHICRQYGAHIYSFSRSENGVDVSDPALVADALRTVMDKEGRIDAVVNTAGVLNRE
PLATMTYEEICKSIHVNYLGAVIVARESYPYLKMSKGALLLFTSSSYTRGRQLYSLYSSSKAAIVNFVQALSEEWQDSGI
RINCINPERTKTPMRERNFGIEPEDTLLKSSVVASVSVNTIFSSLTGEVVDVKKRP
>Mature_456_residues
MRNIAVVLAGGFGQRLGDATPKQFLKLAGKKVIEHTIDVFQNHPLIDEIVVVSNPNYVQEVENILVKNEYSKMKKILKGG
KERYHSSLAAINAYEEEVNMIFHDAVRPLVNDRIISDCINALKTYNAVDVAIKTTDTIIQIDAADHITGIPAREYLRNGQ
TPQAFKRSTIKRAYELALKDVSFQTTDDCGVVYKYLPEEFVYVVKGEQFNMKLTYKEDLFLLDKLFQLKSIAQQNETVTP
KTIAALPSSVIVVFGGSYGIGLDIIHICRQYGAHIYSFSRSENGVDVSDPALVADALRTVMDKEGRIDAVVNTAGVLNRE
PLATMTYEEICKSIHVNYLGAVIVARESYPYLKMSKGALLLFTSSSYTRGRQLYSLYSSSKAAIVNFVQALSEEWQDSGI
RINCINPERTKTPMRERNFGIEPEDTLLKSSVVASVSVNTIFSSLTGEVVDVKKRP

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=233, Percent_Identity=26.1802575107296, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI4503817, Length=221, Percent_Identity=29.8642533936652, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1789104, Length=234, Percent_Identity=26.0683760683761, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87082100, Length=136, Percent_Identity=36.0294117647059, Blast_Score=72, Evalue=6e-14,
Organism=Drosophila melanogaster, GI23397609, Length=196, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 50874; Mature: 50874

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNIAVVLAGGFGQRLGDATPKQFLKLAGKKVIEHTIDVFQNHPLIDEIVVVSNPNYVQE
CCCEEEEEECCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCHHHHH
VENILVKNEYSKMKKILKGGKERYHSSLAAINAYEEEVNMIFHDAVRPLVNDRIISDCIN
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKTYNAVDVAIKTTDTIIQIDAADHITGIPAREYLRNGQTPQAFKRSTIKRAYELALKD
HHHHCCCEEEEEECCCEEEEEECCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VSFQTTDDCGVVYKYLPEEFVYVVKGEQFNMKLTYKEDLFLLDKLFQLKSIAQQNETVTP
CCCCCCCCCHHHHHHCCCCEEEEEECCEEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCC
KTIAALPSSVIVVFGGSYGIGLDIIHICRQYGAHIYSFSRSENGVDVSDPALVADALRTV
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHH
MDKEGRIDAVVNTAGVLNREPLATMTYEEICKSIHVNYLGAVIVARESYPYLKMSKGALL
HCCCCCEEEEEHHHHCCCCCCCHHCCHHHHHHHHHHHHHEEEEEEECCCCCEEECCCEEE
LFTSSSYTRGRQLYSLYSSSKAAIVNFVQALSEEWQDSGIRINCINPERTKTPMRERNFG
EEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEECCHHHCCCHHHCCCC
IEPEDTLLKSSVVASVSVNTIFSSLTGEVVDVKKRP
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
>Mature Secondary Structure
MRNIAVVLAGGFGQRLGDATPKQFLKLAGKKVIEHTIDVFQNHPLIDEIVVVSNPNYVQE
CCCEEEEEECCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCHHHHH
VENILVKNEYSKMKKILKGGKERYHSSLAAINAYEEEVNMIFHDAVRPLVNDRIISDCIN
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKTYNAVDVAIKTTDTIIQIDAADHITGIPAREYLRNGQTPQAFKRSTIKRAYELALKD
HHHHCCCEEEEEECCCEEEEEECCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VSFQTTDDCGVVYKYLPEEFVYVVKGEQFNMKLTYKEDLFLLDKLFQLKSIAQQNETVTP
CCCCCCCCCHHHHHHCCCCEEEEEECCEEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCC
KTIAALPSSVIVVFGGSYGIGLDIIHICRQYGAHIYSFSRSENGVDVSDPALVADALRTV
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHH
MDKEGRIDAVVNTAGVLNREPLATMTYEEICKSIHVNYLGAVIVARESYPYLKMSKGALL
HCCCCCEEEEEHHHHCCCCCCCHHCCHHHHHHHHHHHHHEEEEEEECCCCCEEECCCEEE
LFTSSSYTRGRQLYSLYSSSKAAIVNFVQALSEEWQDSGIRINCINPERTKTPMRERNFG
EEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEECCHHHCCCHHHCCCC
IEPEDTLLKSSVVASVSVNTIFSSLTGEVVDVKKRP
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA