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The map label for this gene is rfbB [H]

Identifier: 150008450

GI number: 150008450

Start: 2183169

End: 2184308

Strand: Direct

Name: rfbB [H]

Synonym: BDI_1834

Alternate gene names: 150008450

Gene position: 2183169-2184308 (Clockwise)

Preceding gene: 150008449

Following gene: 150008464

Centisome position: 45.38

GC content: 47.19

Gene sequence:

>1140_bases
ATGAAAACTTACCTAGTTACCGGTGCCGCCGGTTTTATCGGTGCCAATTTCATCAAACATATGCTGGCGAAATATGACGA
TATCAAGATCGTTGTCCTAGACTTGCTGACTTACGCAGGTAATTTAGGCACCATCGCCGAGGATATCGATGGAGTACGTT
GCGAGTTCGTGAAAGGCGATATTTGCGACCGTGCCTTGGCCGACCAGCTATTCGAGAAATACAACTTCGATTATGTCGTG
AACTTCGCCGCTGAAAGTCATGTAGACCGAAGCATCGAAAATCCCCAGCTATTCCTGCAAACCAACATCCTTGGTACACA
AAACCTATTGGACGCCGCACGAAAAGCATGGGTTACCGGTAAGGCAGAGACTGGTTATCCGGTATGGCGTGAAGGTGTAC
GTTTCCATCAAGTCTCTACCGATGAGGTCTATGGAAGCCTAGGTGCCGAAGGGTATTTCCACGAGACCACGCCTTTAGAT
CCCCGAAGCCCATACAGCGCCTCCAAGACAAGCGCGGACTTATTCGTAAAGGCTTACCACGAGACGTATAAGATGCCGGT
ATCTATTACCCGTTGTTCCAACAACTACGGACCTTACCATTTCCCGGAAAAATTGATTCCTTTAATTATAAAGAACATAT
TGGAAGGTAAATCCCTACCTGTATATGGTGACGGCACAAACGTACGCGACTGGTTATATGTAGAGGATCATTGCAAGGCG
ATCGACGCCGTCATCCACCACGGACGTGTGGGAGAGGTTTACAACGTAGGCGGTCATAACGAGAAGCAGAACATCGAGAT
CGTAAAACTCACGATCCGGACGATCCATCAACTAATGACCGAACAACCGGAATATCGCCAAGTATTAAAGAAAAAAGAGA
TCGGTGCCGATGGAGAAATCTCTATCGACTGGATCAACGACAGCTTGATTACATTCGTAAAAGACCGTCTGGGCCATGAT
CAACGATACGCAATCGACCCGACGAAGATCACCAACGAATTAGGCTGGACACCAGAGACCTGCTTCGAGGTTGGTATCGT
AAAAACCATCCGTTGGTATCTTGAGAACCAGAAATGGGTGGAGGATATCACCGGTGGAGATTACATGAAATATTACGAGC
AAATGTATGGATCACGTTGA

Upstream 100 bases:

>100_bases
ATCACAGATCCGGAATCGATCAACCTATCCGAGAAAGACACCAAGGCTCCATTGCTGAAAGACGCTGAGCTTAATTTCAA
ATACTAAACACTGTACAGAT

Downstream 100 bases:

>100_bases
TTCCTCAACGTGACCGTGTGTATGTGGATGAATCTCAGAGAGGCTGTTTCCTAATTCGGGAGCGGCCTCTCTTATTTGTT
TCCGGATCGTCTCGCGTATC

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKTYLVTGAAGFIGANFIKHMLAKYDDIKIVVLDLLTYAGNLGTIAEDIDGVRCEFVKGDICDRALADQLFEKYNFDYVV
NFAAESHVDRSIENPQLFLQTNILGTQNLLDAARKAWVTGKAETGYPVWREGVRFHQVSTDEVYGSLGAEGYFHETTPLD
PRSPYSASKTSADLFVKAYHETYKMPVSITRCSNNYGPYHFPEKLIPLIIKNILEGKSLPVYGDGTNVRDWLYVEDHCKA
IDAVIHHGRVGEVYNVGGHNEKQNIEIVKLTIRTIHQLMTEQPEYRQVLKKKEIGADGEISIDWINDSLITFVKDRLGHD
QRYAIDPTKITNELGWTPETCFEVGIVKTIRWYLENQKWVEDITGGDYMKYYEQMYGSR

Sequences:

>Translated_379_residues
MKTYLVTGAAGFIGANFIKHMLAKYDDIKIVVLDLLTYAGNLGTIAEDIDGVRCEFVKGDICDRALADQLFEKYNFDYVV
NFAAESHVDRSIENPQLFLQTNILGTQNLLDAARKAWVTGKAETGYPVWREGVRFHQVSTDEVYGSLGAEGYFHETTPLD
PRSPYSASKTSADLFVKAYHETYKMPVSITRCSNNYGPYHFPEKLIPLIIKNILEGKSLPVYGDGTNVRDWLYVEDHCKA
IDAVIHHGRVGEVYNVGGHNEKQNIEIVKLTIRTIHQLMTEQPEYRQVLKKKEIGADGEISIDWINDSLITFVKDRLGHD
QRYAIDPTKITNELGWTPETCFEVGIVKTIRWYLENQKWVEDITGGDYMKYYEQMYGSR
>Mature_379_residues
MKTYLVTGAAGFIGANFIKHMLAKYDDIKIVVLDLLTYAGNLGTIAEDIDGVRCEFVKGDICDRALADQLFEKYNFDYVV
NFAAESHVDRSIENPQLFLQTNILGTQNLLDAARKAWVTGKAETGYPVWREGVRFHQVSTDEVYGSLGAEGYFHETTPLD
PRSPYSASKTSADLFVKAYHETYKMPVSITRCSNNYGPYHFPEKLIPLIIKNILEGKSLPVYGDGTNVRDWLYVEDHCKA
IDAVIHHGRVGEVYNVGGHNEKQNIEIVKLTIRTIHQLMTEQPEYRQVLKKKEIGADGEISIDWINDSLITFVKDRLGHD
QRYAIDPTKITNELGWTPETCFEVGIVKTIRWYLENQKWVEDITGGDYMKYYEQMYGSR

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=356, Percent_Identity=36.5168539325843, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI42516563, Length=358, Percent_Identity=24.3016759776536, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI48994969, Length=373, Percent_Identity=48.2573726541555, Blast_Score=354, Evalue=5e-99,
Organism=Escherichia coli, GI1788353, Length=384, Percent_Identity=46.09375, Blast_Score=347, Evalue=1e-96,
Organism=Escherichia coli, GI1788366, Length=185, Percent_Identity=32.4324324324324, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786974, Length=276, Percent_Identity=26.0869565217391, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=361, Percent_Identity=32.409972299169, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI115532424, Length=369, Percent_Identity=28.9972899728997, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17539532, Length=358, Percent_Identity=22.3463687150838, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=193, Percent_Identity=29.0155440414508, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=193, Percent_Identity=29.0155440414508, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=193, Percent_Identity=29.0155440414508, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=193, Percent_Identity=29.0155440414508, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=193, Percent_Identity=29.0155440414508, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=187, Percent_Identity=28.8770053475936, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=269, Percent_Identity=25.6505576208178, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21356223, Length=358, Percent_Identity=23.1843575418994, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19923002, Length=286, Percent_Identity=28.3216783216783, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24158427, Length=248, Percent_Identity=25, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 43219; Mature: 43219

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYLVTGAAGFIGANFIKHMLAKYDDIKIVVLDLLTYAGNLGTIAEDIDGVRCEFVKGD
CCEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHCCCCEEEEECCH
ICDRALADQLFEKYNFDYVVNFAAESHVDRSIENPQLFLQTNILGTQNLLDAARKAWVTG
HHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCC
KAETGYPVWREGVRFHQVSTDEVYGSLGAEGYFHETTPLDPRSPYSASKTSADLFVKAYH
CCCCCCHHHHCCCEEEECCHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHH
ETYKMPVSITRCSNNYGPYHFPEKLIPLIIKNILEGKSLPVYGDGTNVRDWLYVEDHCKA
HHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEHHHHHH
IDAVIHHGRVGEVYNVGGHNEKQNIEIVKLTIRTIHQLMTEQPEYRQVLKKKEIGADGEI
HHHHHHCCCCCCEEECCCCCCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCE
SIDWINDSLITFVKDRLGHDQRYAIDPTKITNELGWTPETCFEVGIVKTIRWYLENQKWV
EEEEECHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHH
EDITGGDYMKYYEQMYGSR
HHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKTYLVTGAAGFIGANFIKHMLAKYDDIKIVVLDLLTYAGNLGTIAEDIDGVRCEFVKGD
CCEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHCCCCEEEEECCH
ICDRALADQLFEKYNFDYVVNFAAESHVDRSIENPQLFLQTNILGTQNLLDAARKAWVTG
HHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCC
KAETGYPVWREGVRFHQVSTDEVYGSLGAEGYFHETTPLDPRSPYSASKTSADLFVKAYH
CCCCCCHHHHCCCEEEECCHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHH
ETYKMPVSITRCSNNYGPYHFPEKLIPLIIKNILEGKSLPVYGDGTNVRDWLYVEDHCKA
HHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEHHHHHH
IDAVIHHGRVGEVYNVGGHNEKQNIEIVKLTIRTIHQLMTEQPEYRQVLKKKEIGADGEI
HHHHHHCCCCCCEEECCCCCCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCE
SIDWINDSLITFVKDRLGHDQRYAIDPTKITNELGWTPETCFEVGIVKTIRWYLENQKWV
EEEEECHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHH
EDITGGDYMKYYEQMYGSR
HHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]