Click here to switch to the map view.

The map label for this gene is guaA

Identifier: 150008447

GI number: 150008447

Start: 2180318

End: 2181838

Strand: Reverse

Name: guaA

Synonym: BDI_1829

Alternate gene names: 150008447

Gene position: 2181838-2180318 (Counterclockwise)

Preceding gene: 150008448

Following gene: 150008446

Centisome position: 45.35

GC content: 48.26

Gene sequence:

>1521_bases
ATGCACGAGAAACTTATTATTCTTGATTTCGGCTCGCAGACAACCCAGCTTATCGGCCGTAGGGTCAGAGAGTTGAATAT
GTATTGTGAGATCGTTCCTTATAACAAATTCCCGCATGACGCTACCGACGTGAAAGGTGTGATCCTTTCCGGAAGTCCTT
ATTCCGTATACGACGAGAATGCCTTTAAGGCTGATTTGACCGAGATACGGGGGAAGTATCCCGTCTTAGGTATTTGTTAT
GGAGCGCAGTTTCTAGCTTATACATCCGGAGGTAATGTGGAACCGGCTAATTCACGTGAGTATGGACGTGCTAATTTGTC
GCACATTGATGGTGAGGATGAATTACTGAAAGGGATCCATGTGGGTTCACAGATATGGATGTCTCATGGGGATACGATTA
CCGTATTGCCGGATAATTTCAAGGTGATCGCCAGCACGGACGACGTGAGGGCTGCCGCTTACCATGTGGAAGGTGAGCAA
ACGTGGGGCGTTCAGTTTCATCCGGAAGTATTCCATTCGACAGACGGTACGAAGTTGCTGGATAACTTCTTGAATATTTG
CGGATGCGCCAAGGATTGGACACCGGCTTCCTTTATCGAATCTACCGTAGCTGAGTTGAAGGAGCAATTGGGAGATGACA
AGGTGATCTTGGCTCTTTCCGGCGGTGTGGATTCATCTGTTACGGCCGTATTGCTGAATAAGGCGATCGGTAAGAACTTG
ACTTGCATTTTCGTGGATCATGGTTTGCTGCGCAAGAATGAGTTCGAGAACGTAATGAGGGATTATGAGCATTTAGGATT
GAACGTGATCGGCGTAAACGCAAAAGATAAGTTCTATAAAGAACTAGCGGGCGTTACGGAACCGGAGAAGAAACGTAAGA
TCATCGGCAAAGGATTTATCGATGTCTTCGATGAGGAGGCGCATAAGCTGAAAGATATCAAGTGGCTGGGGCAAGGTACG
ATTTATCCGGATGTGATCGAGTCGCTTTCTATCACAGGTACGGTTATCAAGAGCCATCATAATGTGGGCGGACTTCCGGA
TACGATGAACTTGAAACTCGTCGAGCCGCTTCGTCTTCTTTTCAAGGATGAGGTTCGCCGTGTCGGTATGGAGTTGGGTA
TGCAACCGCATTTGATCAAGCGCCATCCGTTCCCGGGACCGGGATTGGGTATCCGTATCTTGGGTGATATCACGCCGGAA
AAGGTACGTATCCTGCAAGACGCTGACGATATCTATATGTCATTGATGCGTGAGTGGGGACTTTACGATAAGATTTGGCA
AGCTGGGGTAATCTTACTTCCGATTCAATCCGTAGGTGTAATGGGCGATGAGCGTACCTATGAGAATACGGTAGCTTTAC
GTGCGGTCACTTCCACGGACGCTATGACCGCCGATTGGGCGCAGCTTCCGTACGAGTTCCTCGCCAAAGTTTCCAACGAG
ATTATCAATAAGGTGAAAGGCGTGAATAGAGTCGTGTACGACATCAGCTCGAAGCCGCCTGCAACCATCGAGTGGGAGTG
A

Upstream 100 bases:

>100_bases
TTTAAAAGTATCGTGATAAGGAAGAATATTTGCCCAGTGTGTTTATGCTTCCCGAATTTATTACTACCTTTGCGCCACTC
AATTAAATAATCATAATTGC

Downstream 100 bases:

>100_bases
CATTTGTCTTAGGTATAACAAAGTGTAAAATCAATAAAATTTAAAAGTCACATGAAGCAAGATATGATTGTTATCTTGGA
TTTAGGTAGTCATGAGAATA

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MHEKLIILDFGSQTTQLIGRRVRELNMYCEIVPYNKFPHDATDVKGVILSGSPYSVYDENAFKADLTEIRGKYPVLGICY
GAQFLAYTSGGNVEPANSREYGRANLSHIDGEDELLKGIHVGSQIWMSHGDTITVLPDNFKVIASTDDVRAAAYHVEGEQ
TWGVQFHPEVFHSTDGTKLLDNFLNICGCAKDWTPASFIESTVAELKEQLGDDKVILALSGGVDSSVTAVLLNKAIGKNL
TCIFVDHGLLRKNEFENVMRDYEHLGLNVIGVNAKDKFYKELAGVTEPEKKRKIIGKGFIDVFDEEAHKLKDIKWLGQGT
IYPDVIESLSITGTVIKSHHNVGGLPDTMNLKLVEPLRLLFKDEVRRVGMELGMQPHLIKRHPFPGPGLGIRILGDITPE
KVRILQDADDIYMSLMREWGLYDKIWQAGVILLPIQSVGVMGDERTYENTVALRAVTSTDAMTADWAQLPYEFLAKVSNE
IINKVKGVNRVVYDISSKPPATIEWE

Sequences:

>Translated_506_residues
MHEKLIILDFGSQTTQLIGRRVRELNMYCEIVPYNKFPHDATDVKGVILSGSPYSVYDENAFKADLTEIRGKYPVLGICY
GAQFLAYTSGGNVEPANSREYGRANLSHIDGEDELLKGIHVGSQIWMSHGDTITVLPDNFKVIASTDDVRAAAYHVEGEQ
TWGVQFHPEVFHSTDGTKLLDNFLNICGCAKDWTPASFIESTVAELKEQLGDDKVILALSGGVDSSVTAVLLNKAIGKNL
TCIFVDHGLLRKNEFENVMRDYEHLGLNVIGVNAKDKFYKELAGVTEPEKKRKIIGKGFIDVFDEEAHKLKDIKWLGQGT
IYPDVIESLSITGTVIKSHHNVGGLPDTMNLKLVEPLRLLFKDEVRRVGMELGMQPHLIKRHPFPGPGLGIRILGDITPE
KVRILQDADDIYMSLMREWGLYDKIWQAGVILLPIQSVGVMGDERTYENTVALRAVTSTDAMTADWAQLPYEFLAKVSNE
IINKVKGVNRVVYDISSKPPATIEWE
>Mature_506_residues
MHEKLIILDFGSQTTQLIGRRVRELNMYCEIVPYNKFPHDATDVKGVILSGSPYSVYDENAFKADLTEIRGKYPVLGICY
GAQFLAYTSGGNVEPANSREYGRANLSHIDGEDELLKGIHVGSQIWMSHGDTITVLPDNFKVIASTDDVRAAAYHVEGEQ
TWGVQFHPEVFHSTDGTKLLDNFLNICGCAKDWTPASFIESTVAELKEQLGDDKVILALSGGVDSSVTAVLLNKAIGKNL
TCIFVDHGLLRKNEFENVMRDYEHLGLNVIGVNAKDKFYKELAGVTEPEKKRKIIGKGFIDVFDEEAHKLKDIKWLGQGT
IYPDVIESLSITGTVIKSHHNVGGLPDTMNLKLVEPLRLLFKDEVRRVGMELGMQPHLIKRHPFPGPGLGIRILGDITPE
KVRILQDADDIYMSLMREWGLYDKIWQAGVILLPIQSVGVMGDERTYENTVALRAVTSTDAMTADWAQLPYEFLAKVSNE
IINKVKGVNRVVYDISSKPPATIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=547, Percent_Identity=34.9177330895795, Blast_Score=276, Evalue=4e-74,
Organism=Escherichia coli, GI1788854, Length=518, Percent_Identity=53.6679536679537, Blast_Score=552, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI71992717, Length=569, Percent_Identity=35.3251318101933, Blast_Score=283, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI71992710, Length=569, Percent_Identity=35.3251318101933, Blast_Score=283, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI133901714, Length=569, Percent_Identity=35.3251318101933, Blast_Score=282, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6323873, Length=517, Percent_Identity=49.9032882011605, Blast_Score=493, Evalue=1e-140,
Organism=Drosophila melanogaster, GI281365319, Length=552, Percent_Identity=34.2391304347826, Blast_Score=261, Evalue=6e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_PARD8 (A6LD04)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303190.1
- ProteinModelPortal:   A6LD04
- STRING:   A6LD04
- GeneID:   5306979
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_1829
- eggNOG:   COG0519
- HOGENOM:   HBG391775
- OMA:   QDICGCE
- ProtClustDB:   PRK00074
- BioCyc:   PDIS435591:BDI_1829-MONOMER
- HAMAP:   MF_00344
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 56417; Mature: 56417

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 79-79 ACT_SITE 167-167 ACT_SITE 169-169

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEKLIILDFGSQTTQLIGRRVRELNMYCEIVPYNKFPHDATDVKGVILSGSPYSVYDEN
CCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEECCCCCEECCCC
AFKADLTEIRGKYPVLGICYGAQFLAYTSGGNVEPANSREYGRANLSHIDGEDELLKGIH
CHHHHHHHHCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
VGSQIWMSHGDTITVLPDNFKVIASTDDVRAAAYHVEGEQTWGVQFHPEVFHSTDGTKLL
CCHHHHCCCCCEEEEECCCCEEEEECCCCEEEEEEECCCEEECEEECHHHHCCCCCHHHH
DNFLNICGCAKDWTPASFIESTVAELKEQLGDDKVILALSGGVDSSVTAVLLNKAIGKNL
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCE
TCIFVDHGLLRKNEFENVMRDYEHLGLNVIGVNAKDKFYKELAGVTEPEKKRKIIGKGFI
EEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCHHHHHHHHCHHH
DVFDEEAHKLKDIKWLGQGTIYPDVIESLSITGTVIKSHHNVGGLPDTMNLKLVEPLRLL
HHHHHHHHHHHHHHEECCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCEEHHHHHHHH
FKDEVRRVGMELGMQPHLIKRHPFPGPGLGIRILGDITPEKVRILQDADDIYMSLMREWG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHCHHHHHHHHHHHCC
LYDKIWQAGVILLPIQSVGVMGDERTYENTVALRAVTSTDAMTADWAQLPYEFLAKVSNE
HHHHHHHCCEEEEEHHHHCCCCCCCCCCCEEEEEEEECCCCCCCHHHHCCHHHHHHHHHH
IINKVKGVNRVVYDISSKPPATIEWE
HHHHHHCCCEEEEEECCCCCCEEECC
>Mature Secondary Structure
MHEKLIILDFGSQTTQLIGRRVRELNMYCEIVPYNKFPHDATDVKGVILSGSPYSVYDEN
CCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEECCCCCEECCCC
AFKADLTEIRGKYPVLGICYGAQFLAYTSGGNVEPANSREYGRANLSHIDGEDELLKGIH
CHHHHHHHHCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
VGSQIWMSHGDTITVLPDNFKVIASTDDVRAAAYHVEGEQTWGVQFHPEVFHSTDGTKLL
CCHHHHCCCCCEEEEECCCCEEEEECCCCEEEEEEECCCEEECEEECHHHHCCCCCHHHH
DNFLNICGCAKDWTPASFIESTVAELKEQLGDDKVILALSGGVDSSVTAVLLNKAIGKNL
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCE
TCIFVDHGLLRKNEFENVMRDYEHLGLNVIGVNAKDKFYKELAGVTEPEKKRKIIGKGFI
EEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCHHHHHHHHCHHH
DVFDEEAHKLKDIKWLGQGTIYPDVIESLSITGTVIKSHHNVGGLPDTMNLKLVEPLRLL
HHHHHHHHHHHHHHEECCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCEEHHHHHHHH
FKDEVRRVGMELGMQPHLIKRHPFPGPGLGIRILGDITPEKVRILQDADDIYMSLMREWG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHCHHHHHHHHHHHCC
LYDKIWQAGVILLPIQSVGVMGDERTYENTVALRAVTSTDAMTADWAQLPYEFLAKVSNE
HHHHHHHCCEEEEEHHHHCCCCCCCCCCCEEEEEEEECCCCCCCHHHHCCHHHHHHHHHH
IINKVKGVNRVVYDISSKPPATIEWE
HHHHHHCCCEEEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA