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The map label for this gene is cysL [H]

Identifier: 150008438

GI number: 150008438

Start: 2171494

End: 2172381

Strand: Reverse

Name: cysL [H]

Synonym: BDI_1820

Alternate gene names: 150008438

Gene position: 2172381-2171494 (Counterclockwise)

Preceding gene: 150008446

Following gene: 150008437

Centisome position: 45.15

GC content: 43.13

Gene sequence:

>888_bases
ATGGATTTTCGATTAAAAGTATTTCATAGCGTAGCTTGTAACCTAAGTTTCACGAAAGCGTCCCGGGAGTTGTTCATTAG
TCAACCCGCTATTAGTAAGCATATCCATGAGTTGGAGGTTCAATATAAGACGCCTCTCTTTGAACGAACAGGTAGCCAGA
TCCGTTTAACTCGTGCGGGCGAGTTGTTGTTTTCGCATACCCATTCCTTATTGGCTTCGTATCGTCAATTGGATTTCGAG
ATGAATCTGTTGACAAATAATTTTCTGGGGGATTTACATTTAGGTGCCAGTACGACAATATCCCAATATGTTCTTCCGCC
CGTGCTTGCTTTATTCATTAAGATGTTTCCGGATATACATGTATCTGTATTGAATGGGAATAGCCGGGATATAGAGTTGG
CTTTGCGAGATGGAAAAATAACGTTAGGATTGGTGGAAGGGACTACCCGTCAAAATACGATGCATTATATACCTTTTATG
AAGGATGAGTTAGTGGTTGTCACTCATGTAGGGTCTAAGTTGGCTGCTTATGACGAACTGACGTTGGAACAACTTTGTGC
TTTGCCCGTAGTACTTCGTGAAAACGGCTCCGGTACGCTTGAGGTCTTGGAGGGGGCTTTAGCAAAACACCAGATCAAAC
TGTCGCAATTGAACGTATTATTGCAGCTAGGTAGTACGGAGAGTATCAAATTATTCTTGGAAAATTCCGATGCTTTGGGA
ATTCTTTCTATTCGGGCGGTGACACGTGAGCTGATGGCTGGACGTTTGAAAGTGATAGATATTGAGGGCTTTAAGGCCGA
ACGAACTTTCTCCTTTGTGGAGCCGCAGGGACAGAACAGCGGTATGGAAGAAAGTTTCATGCGTTTTGCCAGTCAACATT
GGCAATAA

Upstream 100 bases:

>100_bases
ATAGATATTTCATATTTATTCATATATTTATGTTTCAATCCACAAATATATAGTATTATTTTTATCTTTGTTAAATTAAA
ACGGCAAAAGATAGAATATC

Downstream 100 bases:

>100_bases
CTTTAGGTTATTCGCTATAAAAACTTGTGAAGTAAGGGATCGGATGAAAATATGTACTTTCGCGAACGAAATCAAATGAT
GATATGTTGTACTAAATAAA

Product: putative transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MDFRLKVFHSVACNLSFTKASRELFISQPAISKHIHELEVQYKTPLFERTGSQIRLTRAGELLFSHTHSLLASYRQLDFE
MNLLTNNFLGDLHLGASTTISQYVLPPVLALFIKMFPDIHVSVLNGNSRDIELALRDGKITLGLVEGTTRQNTMHYIPFM
KDELVVVTHVGSKLAAYDELTLEQLCALPVVLRENGSGTLEVLEGALAKHQIKLSQLNVLLQLGSTESIKLFLENSDALG
ILSIRAVTRELMAGRLKVIDIEGFKAERTFSFVEPQGQNSGMEESFMRFASQHWQ

Sequences:

>Translated_295_residues
MDFRLKVFHSVACNLSFTKASRELFISQPAISKHIHELEVQYKTPLFERTGSQIRLTRAGELLFSHTHSLLASYRQLDFE
MNLLTNNFLGDLHLGASTTISQYVLPPVLALFIKMFPDIHVSVLNGNSRDIELALRDGKITLGLVEGTTRQNTMHYIPFM
KDELVVVTHVGSKLAAYDELTLEQLCALPVVLRENGSGTLEVLEGALAKHQIKLSQLNVLLQLGSTESIKLFLENSDALG
ILSIRAVTRELMAGRLKVIDIEGFKAERTFSFVEPQGQNSGMEESFMRFASQHWQ
>Mature_295_residues
MDFRLKVFHSVACNLSFTKASRELFISQPAISKHIHELEVQYKTPLFERTGSQIRLTRAGELLFSHTHSLLASYRQLDFE
MNLLTNNFLGDLHLGASTTISQYVLPPVLALFIKMFPDIHVSVLNGNSRDIELALRDGKITLGLVEGTTRQNTMHYIPFM
KDELVVVTHVGSKLAAYDELTLEQLCALPVVLRENGSGTLEVLEGALAKHQIKLSQLNVLLQLGSTESIKLFLENSDALG
ILSIRAVTRELMAGRLKVIDIEGFKAERTFSFVEPQGQNSGMEESFMRFASQHWQ

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=267, Percent_Identity=32.9588014981273, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788748, Length=256, Percent_Identity=25.78125, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI157672245, Length=181, Percent_Identity=27.6243093922652, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI145693105, Length=211, Percent_Identity=23.2227488151659, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788296, Length=247, Percent_Identity=22.6720647773279, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787879, Length=196, Percent_Identity=27.0408163265306, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787601, Length=156, Percent_Identity=28.8461538461538, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788887, Length=209, Percent_Identity=28.2296650717703, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789506, Length=240, Percent_Identity=24.1666666666667, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082132, Length=241, Percent_Identity=24.896265560166, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789639, Length=123, Percent_Identity=30.0813008130081, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1786401, Length=143, Percent_Identity=27.2727272727273, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33116; Mature: 33116

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFRLKVFHSVACNLSFTKASRELFISQPAISKHIHELEVQYKTPLFERTGSQIRLTRAG
CCEEEEEEHHHHCCEEEECCCCEEEEECCHHHHHHHHHEEEECCCHHHCCCCEEEEEEHH
ELLFSHTHSLLASYRQLDFEMNLLTNNFLGDLHLGASTTISQYVLPPVLALFIKMFPDIH
HHHHHHHHHHHHHHHHCCEEEEEHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCEE
VSVLNGNSRDIELALRDGKITLGLVEGTTRQNTMHYIPFMKDELVVVTHVGSKLAAYDEL
EEEECCCCCEEEEEEECCEEEEEEEECCCCCCEEEECCCCCCCEEEEEECCCHHHHHHHH
TLEQLCALPVVLRENGSGTLEVLEGALAKHQIKLSQLNVLLQLGSTESIKLFLENSDALG
HHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEEECCCCEE
ILSIRAVTRELMAGRLKVIDIEGFKAERTFSFVEPQGQNSGMEESFMRFASQHWQ
EHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MDFRLKVFHSVACNLSFTKASRELFISQPAISKHIHELEVQYKTPLFERTGSQIRLTRAG
CCEEEEEEHHHHCCEEEECCCCEEEEECCHHHHHHHHHEEEECCCHHHCCCCEEEEEEHH
ELLFSHTHSLLASYRQLDFEMNLLTNNFLGDLHLGASTTISQYVLPPVLALFIKMFPDIH
HHHHHHHHHHHHHHHHCCEEEEEHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCEE
VSVLNGNSRDIELALRDGKITLGLVEGTTRQNTMHYIPFMKDELVVVTHVGSKLAAYDEL
EEEECCCCCEEEEEEECCEEEEEEEECCCCCCEEEECCCCCCCEEEEEECCCHHHHHHHH
TLEQLCALPVVLRENGSGTLEVLEGALAKHQIKLSQLNVLLQLGSTESIKLFLENSDALG
HHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEEECCCCEE
ILSIRAVTRELMAGRLKVIDIEGFKAERTFSFVEPQGQNSGMEESFMRFASQHWQ
EHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]