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The map label for this gene is fmt

Identifier: 150008368

GI number: 150008368

Start: 2064695

End: 2065669

Strand: Reverse

Name: fmt

Synonym: BDI_1742

Alternate gene names: 150008368

Gene position: 2065669-2064695 (Counterclockwise)

Preceding gene: 150008369

Following gene: 150008367

Centisome position: 42.93

GC content: 51.69

Gene sequence:

>975_bases
ATGAAGAAAGAGGATTTACGAATCGTATATATGGGCACTCCCGATTTCGCCGTGGAGAGCCTGCGTGCCTTGGTAGAAGG
TGGATATAATGTGGTAGGTGTCATTACGATGCCGGATAAGCCGATGGGCCGTCATGGTAGCGTGCTTCAGCCGAGTGCCG
TGAAGCAATATGCCGTATCGGTGGGTCTTCCGGTCTTACAGCCAGAGAAGTTGAAGGATGAGGCGTTTTTGGAGGAACTG
CGTGCCTTGCGGGCGGATTTGCAGATCGTGGTGGCGTTTCGTATGCTGCCGGAGGTGGTCTGGAATATGCCCCGTCTGGG
AACTTTTAATCTGCATGCCTCCTTGCTTCCGCAATACCGGGGTGCCGCTCCGATCAACTGGGCGGTTATCAATGGTGATA
CGGAGACCGGCGTGACTACTTTCTTTCTTACGCATGAGATCGATACGGGTAAGATCATCCGTCAGAGACATCTGCCGATC
GCTGATACCGACGATGTAGAAACCGTTCACGATGCCTTGATGGCGATGGGGGCGAGGTTGGTTACCGAGACCGTGGACTT
ACTGTTGGATGGAAAAACAGACGCTATCCCCCAAGAGGAATTCTATAAGGATGCCGCCGAGTTGCGTCCGGCCCCGAAGA
TATTCAAGGATACCTGTCATATCGACTGGAACCAGCCGGTGAAGAATATCTATGATTTCGTCCGTGGGCTTTCTCCTTAT
CCTGCCGCTTGGACGGAATTGATCTCTGAGGATGGCAAACGTCTGGCTTTAAAGATTTACCAGGCAGAGAAACGTCCGGC
GGAGCATAACTTCCCGGTCGGCTCTATCCATACGGATCATAAATCGTATATCGACGTGGCCGTGAAGGACGGTTTTCTTC
GCTTGCGTTCCTTGCAACTTGCCGGAAAGAAACGGATGAATATAACGGACTTCCTGAATGGTTTCAAGCAAATTGCGGAA
TATACGGTTGGCTGA

Upstream 100 bases:

>100_bases
CTTTATGTCGAAATCGAAGATATTTAATTCCTATCGGGAGGTATTGGTCGATAACTTTTCCGGGGACTAACTTGTTAAAA
ACAGATCCAATTTATTGTAC

Downstream 100 bases:

>100_bases
TGAACGGGACGAGACGTATCATATACATTACGTTAGGAACGTTTTTTCTCATATTGGGAGCGATCGGTATCTTTGTCCCT
TTGCTCCCGACCACTCCTTT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKKEDLRIVYMGTPDFAVESLRALVEGGYNVVGVITMPDKPMGRHGSVLQPSAVKQYAVSVGLPVLQPEKLKDEAFLEEL
RALRADLQIVVAFRMLPEVVWNMPRLGTFNLHASLLPQYRGAAPINWAVINGDTETGVTTFFLTHEIDTGKIIRQRHLPI
ADTDDVETVHDALMAMGARLVTETVDLLLDGKTDAIPQEEFYKDAAELRPAPKIFKDTCHIDWNQPVKNIYDFVRGLSPY
PAAWTELISEDGKRLALKIYQAEKRPAEHNFPVGSIHTDHKSYIDVAVKDGFLRLRSLQLAGKKRMNITDFLNGFKQIAE
YTVG

Sequences:

>Translated_324_residues
MKKEDLRIVYMGTPDFAVESLRALVEGGYNVVGVITMPDKPMGRHGSVLQPSAVKQYAVSVGLPVLQPEKLKDEAFLEEL
RALRADLQIVVAFRMLPEVVWNMPRLGTFNLHASLLPQYRGAAPINWAVINGDTETGVTTFFLTHEIDTGKIIRQRHLPI
ADTDDVETVHDALMAMGARLVTETVDLLLDGKTDAIPQEEFYKDAAELRPAPKIFKDTCHIDWNQPVKNIYDFVRGLSPY
PAAWTELISEDGKRLALKIYQAEKRPAEHNFPVGSIHTDHKSYIDVAVKDGFLRLRSLQLAGKKRMNITDFLNGFKQIAE
YTVG
>Mature_324_residues
MKKEDLRIVYMGTPDFAVESLRALVEGGYNVVGVITMPDKPMGRHGSVLQPSAVKQYAVSVGLPVLQPEKLKDEAFLEEL
RALRADLQIVVAFRMLPEVVWNMPRLGTFNLHASLLPQYRGAAPINWAVINGDTETGVTTFFLTHEIDTGKIIRQRHLPI
ADTDDVETVHDALMAMGARLVTETVDLLLDGKTDAIPQEEFYKDAAELRPAPKIFKDTCHIDWNQPVKNIYDFVRGLSPY
PAAWTELISEDGKRLALKIYQAEKRPAEHNFPVGSIHTDHKSYIDVAVKDGFLRLRSLQLAGKKRMNITDFLNGFKQIAE
YTVG

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI21614513, Length=325, Percent_Identity=26.7692307692308, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI238814322, Length=247, Percent_Identity=30.3643724696356, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI164663775, Length=330, Percent_Identity=27.8787878787879, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1789683, Length=316, Percent_Identity=39.2405063291139, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1788589, Length=242, Percent_Identity=28.5123966942149, Blast_Score=114, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI133930964, Length=274, Percent_Identity=28.1021897810219, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI45550868, Length=312, Percent_Identity=30.4487179487179, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571984, Length=233, Percent_Identity=32.1888412017167, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24585660, Length=261, Percent_Identity=27.2030651340996, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_PARD8 (A6LCS5)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303111.1
- ProteinModelPortal:   A6LCS5
- SMR:   A6LCS5
- STRING:   A6LCS5
- GeneID:   5306893
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_1742
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   VVAYGAI
- ProtClustDB:   PRK00005
- BioCyc:   PDIS435591:BDI_1742-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 36367; Mature: 36367

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEDLRIVYMGTPDFAVESLRALVEGGYNVVGVITMPDKPMGRHGSVLQPSAVKQYAVS
CCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
VGLPVLQPEKLKDEAFLEELRALRADLQIVVAFRMLPEVVWNMPRLGTFNLHASLLPQYR
HCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHC
GAAPINWAVINGDTETGVTTFFLTHEIDTGKIIRQRHLPIADTDDVETVHDALMAMGARL
CCCCCEEEEECCCCCCCEEEEEEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
VTETVDLLLDGKTDAIPQEEFYKDAAELRPAPKIFKDTCHIDWNQPVKNIYDFVRGLSPY
HHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCC
PAAWTELISEDGKRLALKIYQAEKRPAEHNFPVGSIHTDHKSYIDVAVKDGFLRLRSLQL
CHHHHHHHHCCCCEEEEEEEHHCCCCCCCCCCCCCEECCCHHHEEEEEHHHHHHHHHHHH
AGKKRMNITDFLNGFKQIAEYTVG
HCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKEDLRIVYMGTPDFAVESLRALVEGGYNVVGVITMPDKPMGRHGSVLQPSAVKQYAVS
CCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
VGLPVLQPEKLKDEAFLEELRALRADLQIVVAFRMLPEVVWNMPRLGTFNLHASLLPQYR
HCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHC
GAAPINWAVINGDTETGVTTFFLTHEIDTGKIIRQRHLPIADTDDVETVHDALMAMGARL
CCCCCEEEEECCCCCCCEEEEEEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
VTETVDLLLDGKTDAIPQEEFYKDAAELRPAPKIFKDTCHIDWNQPVKNIYDFVRGLSPY
HHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCC
PAAWTELISEDGKRLALKIYQAEKRPAEHNFPVGSIHTDHKSYIDVAVKDGFLRLRSLQL
CHHHHHHHHCCCCEEEEEEEHHCCCCCCCCCCCCCEECCCHHHEEEEEHHHHHHHHHHHH
AGKKRMNITDFLNGFKQIAEYTVG
HCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA