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The map label for this gene is sbcD [H]

Identifier: 150008356

GI number: 150008356

Start: 2047964

End: 2049214

Strand: Reverse

Name: sbcD [H]

Synonym: BDI_1730

Alternate gene names: 150008356

Gene position: 2049214-2047964 (Counterclockwise)

Preceding gene: 150008357

Following gene: 150008355

Centisome position: 42.59

GC content: 60.91

Gene sequence:

>1251_bases
ATGGCATTGAAACTTATCCATACGGCCGACTGGCATCTGGGCCAGACCTTTTTCGGCTACGACCGGGAGGCGGAGCACGA
GGCTTTCCTCGGTTTTCTGACAAATCTATTGGTGGAGCGGGAAACGGATGTCCTGTTGATCGCCGGCGACGTGTTCGACG
TGACGAACCCTTCCGCCGGGGCGCAACGCCGTTTCTATCGTTTCCTGCGGGAGGCGAACCGCCTGAACCCGGGCTTGCAG
ATCGTGATCATCGCCGGCAACCACGACTCCGCGATCCGTCTGGAAGCCCCGAATCCCTTGCTGGAGGAATTGAATACTTC
GATTGTCGGTATCGTGGGGAGGACGGATTCCGGGGAGATCGATCTCGCCTCGCTCGTCGTCCCCTTGAGGAACCGGGCGG
GAGAACGGGAGGCGCTTTGCCTGGCGGTGCCTTTCCTCCGCCAAGGGGATTACCCGGCGGCGCCCGAGGGAGAGCTTGAT
TCGTATGTGGCGGGCATCGGCCGGATGTACCGGCGGCTCTACGCCTATGCCGATGCCCAGAGGAATCCCGGCGAGGCGAT
CGTAGCCTTGGGACACCTCCACGCTACCGGAGCCGAGTTGTCGGACGACGACCGGAGCGAGCGCGCCATCATGGGAGGTC
TGGAGTCCGTATCCGCGGATACCTTCGACGCCGGGATCGCTTATACGGCGCTGGGGCATATCCATAAAGCGCAACGGATC
GGCGGGCGCGAGGCGGTACGTTATGCCGGAAGCCCGCTTCCCATGTCGTTCTCGGAGAAGAATTACCGGCATCAAGTGAT
AGCTGTAGAGATCGAGGAGGGGAGTGTCACGGAGATCGAGGCGATCGGGATTCCTAGAGTCGCGGACTTGATGCGTATAC
CGGACAGCCCGTTACCTCCGAAGGAGGTCTTGCGATGCCTCGCCGGGCTTCCGGAGCCGGAGGAGGGCAGGGAGGACGAG
AGCCGCTGGCCTTATTTGGAGATACGGGTCCTGCTGACGGAGCCGGACCCTACGTTCCGCCATCGGGTAGAGGAGGCGTT
GGTCGGGAAGGCGGTCCGCCTCACCTCCATCGTTCCTTCGTATCCCCGGAGGGAAGGGGAGGCCGAGGAGAGGGCCTTAT
CCTATAATGATTTGCAAAAGATAGCCCCGTTGGATATGCTGAGGCATACGTTTGCAGCGAGATACGGAGGCGAGTTGCCC
GAGGAGATAGAGACATTGTTTAACGAGGTGATAAGGGAGGTCGCTTTATGA

Upstream 100 bases:

>100_bases
AAGGGGGGATACGTTTGATCATTCGTTTTTTTCATTCTACCAAAATCTGATATCTGATGGGAAAGAAGTACATTTGCCGG
ATCAAAATAGAAGAAGATAA

Downstream 100 bases:

>100_bases
GAGTGCTGGCGATCAGAGGACGCAACCTTGCGTCGTTGGAAGGAGAGTTCTCCATTGATTTTACGGAAGAGCCTTTGGCT
TCGGCGGGTATCTTCGCTAT

Product: putative exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MALKLIHTADWHLGQTFFGYDREAEHEAFLGFLTNLLVERETDVLLIAGDVFDVTNPSAGAQRRFYRFLREANRLNPGLQ
IVIIAGNHDSAIRLEAPNPLLEELNTSIVGIVGRTDSGEIDLASLVVPLRNRAGEREALCLAVPFLRQGDYPAAPEGELD
SYVAGIGRMYRRLYAYADAQRNPGEAIVALGHLHATGAELSDDDRSERAIMGGLESVSADTFDAGIAYTALGHIHKAQRI
GGREAVRYAGSPLPMSFSEKNYRHQVIAVEIEEGSVTEIEAIGIPRVADLMRIPDSPLPPKEVLRCLAGLPEPEEGREDE
SRWPYLEIRVLLTEPDPTFRHRVEEALVGKAVRLTSIVPSYPRREGEAEERALSYNDLQKIAPLDMLRHTFAARYGGELP
EEIETLFNEVIREVAL

Sequences:

>Translated_416_residues
MALKLIHTADWHLGQTFFGYDREAEHEAFLGFLTNLLVERETDVLLIAGDVFDVTNPSAGAQRRFYRFLREANRLNPGLQ
IVIIAGNHDSAIRLEAPNPLLEELNTSIVGIVGRTDSGEIDLASLVVPLRNRAGEREALCLAVPFLRQGDYPAAPEGELD
SYVAGIGRMYRRLYAYADAQRNPGEAIVALGHLHATGAELSDDDRSERAIMGGLESVSADTFDAGIAYTALGHIHKAQRI
GGREAVRYAGSPLPMSFSEKNYRHQVIAVEIEEGSVTEIEAIGIPRVADLMRIPDSPLPPKEVLRCLAGLPEPEEGREDE
SRWPYLEIRVLLTEPDPTFRHRVEEALVGKAVRLTSIVPSYPRREGEAEERALSYNDLQKIAPLDMLRHTFAARYGGELP
EEIETLFNEVIREVAL
>Mature_415_residues
ALKLIHTADWHLGQTFFGYDREAEHEAFLGFLTNLLVERETDVLLIAGDVFDVTNPSAGAQRRFYRFLREANRLNPGLQI
VIIAGNHDSAIRLEAPNPLLEELNTSIVGIVGRTDSGEIDLASLVVPLRNRAGEREALCLAVPFLRQGDYPAAPEGELDS
YVAGIGRMYRRLYAYADAQRNPGEAIVALGHLHATGAELSDDDRSERAIMGGLESVSADTFDAGIAYTALGHIHKAQRIG
GREAVRYAGSPLPMSFSEKNYRHQVIAVEIEEGSVTEIEAIGIPRVADLMRIPDSPLPPKEVLRCLAGLPEPEEGREDES
RWPYLEIRVLLTEPDPTFRHRVEEALVGKAVRLTSIVPSYPRREGEAEERALSYNDLQKIAPLDMLRHTFAARYGGELPE
EIETLFNEVIREVAL

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=398, Percent_Identity=32.4120603015075, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 46151; Mature: 46020

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKLIHTADWHLGQTFFGYDREAEHEAFLGFLTNLLVERETDVLLIAGDVFDVTNPSAG
CEEEEEECCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCCCCH
AQRRFYRFLREANRLNPGLQIVIIAGNHDSAIRLEAPNPLLEELNTSIVGIVGRTDSGEI
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCHHHHHCCCCEEEEEECCCCCCE
DLASLVVPLRNRAGEREALCLAVPFLRQGDYPAAPEGELDSYVAGIGRMYRRLYAYADAQ
EHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
RNPGEAIVALGHLHATGAELSDDDRSERAIMGGLESVSADTFDAGIAYTALGHIHKAQRI
CCCCCEEEEEEEEECCCCCCCCCCHHHHHHHCCHHHCCCCCHHCCHHHHHHHHHHHHHHC
GGREAVRYAGSPLPMSFSEKNYRHQVIAVEIEEGSVTEIEAIGIPRVADLMRIPDSPLPP
CCHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCHHHHHHHCCCCCCCH
KEVLRCLAGLPEPEEGREDESRWPYLEIRVLLTEPDPTFRHRVEEALVGKAVRLTSIVPS
HHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHCCC
YPRREGEAEERALSYNDLQKIAPLDMLRHTFAARYGGELPEEIETLFNEVIREVAL
CCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALKLIHTADWHLGQTFFGYDREAEHEAFLGFLTNLLVERETDVLLIAGDVFDVTNPSAG
EEEEEECCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCCCCH
AQRRFYRFLREANRLNPGLQIVIIAGNHDSAIRLEAPNPLLEELNTSIVGIVGRTDSGEI
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCHHHHHCCCCEEEEEECCCCCCE
DLASLVVPLRNRAGEREALCLAVPFLRQGDYPAAPEGELDSYVAGIGRMYRRLYAYADAQ
EHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
RNPGEAIVALGHLHATGAELSDDDRSERAIMGGLESVSADTFDAGIAYTALGHIHKAQRI
CCCCCEEEEEEEEECCCCCCCCCCHHHHHHHCCHHHCCCCCHHCCHHHHHHHHHHHHHHC
GGREAVRYAGSPLPMSFSEKNYRHQVIAVEIEEGSVTEIEAIGIPRVADLMRIPDSPLPP
CCHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCHHHHHHHCCCCCCCH
KEVLRCLAGLPEPEEGREDESRWPYLEIRVLLTEPDPTFRHRVEEALVGKAVRLTSIVPS
HHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHCCC
YPRREGEAEERALSYNDLQKIAPLDMLRHTFAARYGGELPEEIETLFNEVIREVAL
CCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9256491 [H]