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The map label for this gene is pycB [H]

Identifier: 150008301

GI number: 150008301

Start: 1974167

End: 1975744

Strand: Reverse

Name: pycB [H]

Synonym: BDI_1670

Alternate gene names: 150008301

Gene position: 1975744-1974167 (Counterclockwise)

Preceding gene: 150008302

Following gene: 150008294

Centisome position: 41.06

GC content: 48.86

Gene sequence:

>1578_bases
ATGATACGTAAATTATTGATTCGTGATTTGACTCTTCGCGATGGTCAGCAATCGGCATTCGCTACAAGAATGTCTCAAGC
GCAAATAGACCGTGTACTTCCTTACTACAAAGACGCAGGCTTTTATGCCATGGAAGTATGGGGTGGGGCGGTTCCCGATT
CCGTGATGCGTTATTTAGGTGAGAATCCGTGGGATCGTTTAGAGATCATCAAAGAGAAAATAGGAAATTCCTCCAAGTTA
TCCGCTCTTTCCCGTGGGCGTAATTTATTCGGTTATAATCCTTATCCCGACGAGATTATAGAAGGCTTTTGCCGGAACTC
GATTCGTTCTGGTATCGATATCATGCGTATTTTCGATGCTTTGAACGACGTGGATAACGTGAAATCCACGATCCGTTATG
TGAAGAAATTCGGGGGAAAGGCTGACTGTGCCATCTGTTATACGATAGATCCTCACCATTCCCCGGTAGAACGTATAAAA
GCCGCCTTGCATGGACGGCCCTTGCATAAGCCTGTTTTTACGAATGAGTATTTCTTAAACAAGGCCCTTCAACTGGAGTC
GCTGGGCGCTGATATGATCACCCTGAAGGATATGAGTGGTTTGATACCTCCGGCTCGTACGGCGGAATTGGTGCGATTGT
TCAAGAAGAGCCTGAAGGTACCGGTGGATTTCCATACGCATTGTACGCCCGGTTACGGATTGGCGTCGGTCGTATCCGCC
ATTATCAATGGGGTGGACATCGTTGATACGAATATCTGGAACTTCGCCGGAGGACCGGCCGCTCCCGCCGTGGAGCTGGT
CTATATCTTCTGCAAGAAATTGGGTATCGAGCTGGATCTTGATATGGACGCGATCGCTAAGATCAACAAGGAGTTATTGA
CGATCCGCAAGGAGCTTTCCGTGTTCGATACCGCGAAGAAATTCCCGAGGCCCTTTAATCCCGTAGAGGATAGTTTCCCC
GCCGAGATCGACCGCTTCTTCAACGATGCCATCGAGGCCGCCCGGAAAGATAAGGAGGACGATTTGCTGCTTTATTGCCG
TGCGATCGAGGAGTATTTTGATTTCCCCGAGCCGAACGAGTTGGTGAAAAAAGCGCAGATCCCGGGAGGTATGTATACGA
ATATGGTTGCCCAGTTGAAGCAATTGGGGCAGATCGACTTGTTGGAGAAGGCGATGTCGCTCATTCCGCAAGTACGGATG
GATGCCGGCTTACCTCCGTTGGTGACGCCTACGAGCCAGATTATCGGGGCGCAAGCGGTTTCCTGTGCGCTGGATGAGCT
GAAAGGTCGCCCGATGTATAGTAATCCTTCCAACCAGTTTATCGCCTTGGTAAAAGGAGAGTATGGCAAGACGCCGATTC
CGGTAGATCCGGCGTTTCGGTTGAAAATCGCCGGCGTACAAAATGAGGTGCCTTACGACGGTTCCCATTACGTGATGCAA
GAGAATCCTGTATTGGAGGATTTGGATGTGCTATTAGCTGAGAATGAGAAAGAAATACTGTTGCTGGAGTTGTTCCCGAC
GGTAGCCCGTACATTTTTAACTAAATGGAAAGAACAAAAAGCGAGATCGACTGTTTAA

Upstream 100 bases:

>100_bases
AGAAAAATTGGTTATTATTAACCCCGTAATCTTTATCAAATTTGTTTAGATCTTGTTATATAATCAGAATATTGACTAAA
ACGATCTAAACAAATTTGGT

Downstream 100 bases:

>100_bases
TAGTAGAAAGTCCTCAAATTTGGCATCCGTCATCTATTATATGTACGTTGCCGTTTAAACGGTGATACGAAAAGGCTCTC
CGAATGGTAAGCGAACCATG

Product: carboxylase

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MIRKLLIRDLTLRDGQQSAFATRMSQAQIDRVLPYYKDAGFYAMEVWGGAVPDSVMRYLGENPWDRLEIIKEKIGNSSKL
SALSRGRNLFGYNPYPDEIIEGFCRNSIRSGIDIMRIFDALNDVDNVKSTIRYVKKFGGKADCAICYTIDPHHSPVERIK
AALHGRPLHKPVFTNEYFLNKALQLESLGADMITLKDMSGLIPPARTAELVRLFKKSLKVPVDFHTHCTPGYGLASVVSA
IINGVDIVDTNIWNFAGGPAAPAVELVYIFCKKLGIELDLDMDAIAKINKELLTIRKELSVFDTAKKFPRPFNPVEDSFP
AEIDRFFNDAIEAARKDKEDDLLLYCRAIEEYFDFPEPNELVKKAQIPGGMYTNMVAQLKQLGQIDLLEKAMSLIPQVRM
DAGLPPLVTPTSQIIGAQAVSCALDELKGRPMYSNPSNQFIALVKGEYGKTPIPVDPAFRLKIAGVQNEVPYDGSHYVMQ
ENPVLEDLDVLLAENEKEILLLELFPTVARTFLTKWKEQKARSTV

Sequences:

>Translated_525_residues
MIRKLLIRDLTLRDGQQSAFATRMSQAQIDRVLPYYKDAGFYAMEVWGGAVPDSVMRYLGENPWDRLEIIKEKIGNSSKL
SALSRGRNLFGYNPYPDEIIEGFCRNSIRSGIDIMRIFDALNDVDNVKSTIRYVKKFGGKADCAICYTIDPHHSPVERIK
AALHGRPLHKPVFTNEYFLNKALQLESLGADMITLKDMSGLIPPARTAELVRLFKKSLKVPVDFHTHCTPGYGLASVVSA
IINGVDIVDTNIWNFAGGPAAPAVELVYIFCKKLGIELDLDMDAIAKINKELLTIRKELSVFDTAKKFPRPFNPVEDSFP
AEIDRFFNDAIEAARKDKEDDLLLYCRAIEEYFDFPEPNELVKKAQIPGGMYTNMVAQLKQLGQIDLLEKAMSLIPQVRM
DAGLPPLVTPTSQIIGAQAVSCALDELKGRPMYSNPSNQFIALVKGEYGKTPIPVDPAFRLKIAGVQNEVPYDGSHYVMQ
ENPVLEDLDVLLAENEKEILLLELFPTVARTFLTKWKEQKARSTV
>Mature_525_residues
MIRKLLIRDLTLRDGQQSAFATRMSQAQIDRVLPYYKDAGFYAMEVWGGAVPDSVMRYLGENPWDRLEIIKEKIGNSSKL
SALSRGRNLFGYNPYPDEIIEGFCRNSIRSGIDIMRIFDALNDVDNVKSTIRYVKKFGGKADCAICYTIDPHHSPVERIK
AALHGRPLHKPVFTNEYFLNKALQLESLGADMITLKDMSGLIPPARTAELVRLFKKSLKVPVDFHTHCTPGYGLASVVSA
IINGVDIVDTNIWNFAGGPAAPAVELVYIFCKKLGIELDLDMDAIAKINKELLTIRKELSVFDTAKKFPRPFNPVEDSFP
AEIDRFFNDAIEAARKDKEDDLLLYCRAIEEYFDFPEPNELVKKAQIPGGMYTNMVAQLKQLGQIDLLEKAMSLIPQVRM
DAGLPPLVTPTSQIIGAQAVSCALDELKGRPMYSNPSNQFIALVKGEYGKTPIPVDPAFRLKIAGVQNEVPYDGSHYVMQ
ENPVLEDLDVLLAENEKEILLLELFPTVARTFLTKWKEQKARSTV

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=415, Percent_Identity=28.1927710843373, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI106049295, Length=415, Percent_Identity=28.1927710843373, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI106049292, Length=415, Percent_Identity=28.1927710843373, Blast_Score=152, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17562816, Length=531, Percent_Identity=25.4237288135593, Blast_Score=169, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6321376, Length=414, Percent_Identity=28.5024154589372, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6319695, Length=414, Percent_Identity=28.0193236714976, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI281363050, Length=417, Percent_Identity=28.7769784172662, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24652224, Length=417, Percent_Identity=28.7769784172662, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24652222, Length=417, Percent_Identity=28.7769784172662, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24652220, Length=417, Percent_Identity=28.7769784172662, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24652218, Length=417, Percent_Identity=28.7769784172662, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24652212, Length=417, Percent_Identity=28.7769784172662, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24652210, Length=417, Percent_Identity=28.7769784172662, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24652214, Length=417, Percent_Identity=28.7769784172662, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI19921944, Length=417, Percent_Identity=28.7769784172662, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24652216, Length=417, Percent_Identity=28.7769784172662, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 58896; Mature: 58896

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKLLIRDLTLRDGQQSAFATRMSQAQIDRVLPYYKDAGFYAMEVWGGAVPDSVMRYLG
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHCCCEEEEEHHCCCCHHHHHHHHC
ENPWDRLEIIKEKIGNSSKLSALSRGRNLFGYNPYPDEIIEGFCRNSIRSGIDIMRIFDA
CCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
LNDVDNVKSTIRYVKKFGGKADCAICYTIDPHHSPVERIKAALHGRPLHKPVFTNEYFLN
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHH
KALQLESLGADMITLKDMSGLIPPARTAELVRLFKKSLKVPVDFHTHCTPGYGLASVVSA
HHHHHHHCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
IINGVDIVDTNIWNFAGGPAAPAVELVYIFCKKLGIELDLDMDAIAKINKELLTIRKELS
HHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHH
VFDTAKKFPRPFNPVEDSFPAEIDRFFNDAIEAARKDKEDDLLLYCRAIEEYFDFPEPNE
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHH
LVKKAQIPGGMYTNMVAQLKQLGQIDLLEKAMSLIPQVRMDAGLPPLVTPTSQIIGAQAV
HHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
SCALDELKGRPMYSNPSNQFIALVKGEYGKTPIPVDPAFRLKIAGVQNEVPYDGSHYVMQ
HHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEE
ENPVLEDLDVLLAENEKEILLLELFPTVARTFLTKWKEQKARSTV
CCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIRKLLIRDLTLRDGQQSAFATRMSQAQIDRVLPYYKDAGFYAMEVWGGAVPDSVMRYLG
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHCCCEEEEEHHCCCCHHHHHHHHC
ENPWDRLEIIKEKIGNSSKLSALSRGRNLFGYNPYPDEIIEGFCRNSIRSGIDIMRIFDA
CCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
LNDVDNVKSTIRYVKKFGGKADCAICYTIDPHHSPVERIKAALHGRPLHKPVFTNEYFLN
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHH
KALQLESLGADMITLKDMSGLIPPARTAELVRLFKKSLKVPVDFHTHCTPGYGLASVVSA
HHHHHHHCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
IINGVDIVDTNIWNFAGGPAAPAVELVYIFCKKLGIELDLDMDAIAKINKELLTIRKELS
HHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHH
VFDTAKKFPRPFNPVEDSFPAEIDRFFNDAIEAARKDKEDDLLLYCRAIEEYFDFPEPNE
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHH
LVKKAQIPGGMYTNMVAQLKQLGQIDLLEKAMSLIPQVRMDAGLPPLVTPTSQIIGAQAV
HHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
SCALDELKGRPMYSNPSNQFIALVKGEYGKTPIPVDPAFRLKIAGVQNEVPYDGSHYVMQ
HHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEE
ENPVLEDLDVLLAENEKEILLLELFPTVARTFLTKWKEQKARSTV
CCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]