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The map label for this gene is norM [H]

Identifier: 150008020

GI number: 150008020

Start: 1603518

End: 1604864

Strand: Direct

Name: norM [H]

Synonym: BDI_1383

Alternate gene names: 150008020

Gene position: 1603518-1604864 (Clockwise)

Preceding gene: 150008017

Following gene: 150008022

Centisome position: 33.33

GC content: 45.29

Gene sequence:

>1347_bases
ATGTACAATAGAATAGCGGGTTACAAACAATATTATAAATCCTTAGTAATGCTAGGGGTACCTATCATCATTGGACAATT
GGGGGCTATCATCACCGGATTGGCAGACACCATCATGGTGGGACAACACAGTACGGAGGAACTAGCGGCGGCATCGTTCG
TCAATAATGTAATAAACGCATTTATCATAATGGGAACCGGATTCTCCTATAATCTTACCCCGCTGATCGGACAGAATTTA
GCGATGAATAAAAAGATCGTGATCGGAGGCTGGCTCAAAAACAGTCTGGTTGCCAATATGACTACCACCTTATTATTGAT
AGCCGTCTTACTGGGGATTTACCTAAACCTAGACTCTTTAGGCCAACCCGAAGAACTCTTGCCATTGATAAGACCTTATT
TCGCAGTCTCATTAGTCTCGATCATATTCGTAATGCTTTATAATTCATTCCGTCAATTCGTGGAAGGCATAGCCAACCCA
TCCATATCCATGTGGGTCCTATTGACAGGGAATCTGCTGAATATCATCGGCAACTATATCCTCATTTACGGAAAATGCGG
AATGCCGGAACTCGGACTGCTCGGGGCAGGTATAAGCACCTTTATCTCGCGTATCATCATGCTATTGCTTTTCATCGCCG
TATTCATGCTGCGAACCGACTATAAGCCTTACCGGAAAGGTTTCACCCATATTCGTATCAATAAGCAAAGTTGGAGAAGG
CTGAATTCCATAGGCTGGCCGATCAGTCTGCAACAAGGCTTGGAAGCGGGAACATTCTGCGTCACCGCCATCATGGTGGG
TTGGCTGGGTAGCCTTGAGCTTGCCGCCCATCAAATAGCCATTACCATATCAACGATCAGTTTTACCATCTATTTAGGTC
TTGGAGCCGCCGTAGCGATTCGTACCAGTTATTATAAAGGGGCGAACGACTGGTATATGGTGCGTAAGATCACCATTGCC
GGGCTTCATCTAGGGCTTATTGTCGTTTGTATTGTCTGCACCACCTTATTCATGACCCAATCCTGGCTTGGATTAATATT
TACGGATAATCTTCATGTCAACGCAATCGTGAAGACACTACTCCCCATATTGATGCTCTATCAAATAGGAGACAGCGCAC
AAATCATTTTAGCGAACTCCTTACGAGGGCTAGCCGACGTGAAAGCGATTATGTGGATCTCGTTTCTCGCTTATTTCATC
ATAGCCATACCTGCCGGATATCTATTGGGATTCACATTCCATTGGGGTATCGCCGGGATTTGGATGGCTTACCCATTAGG
CTTCTTATGCTCTGTATGCCTGCTGGGATACCGGGCGAAAATGCAGGTAAAATCGCATCTCGCCTAA

Upstream 100 bases:

>100_bases
TCTAATTTATCTTTACATTTTCATTTAAAACAACTCTATCGTATATCCGGCATATACGATCAAGGATTTTTACACCATCA
TTAATAACAGAACAATACAT

Downstream 100 bases:

>100_bases
TCCGATCAAGCCTTACGATTGATCCGGGAGAAACTAATAACCGCCCCGATCAAGGCCAACACACTTCCCAGCCATAACGC
CGCGGGCATGCTATGGCCGG

Product: putative multidrug efflux transport protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter; Na(+)/drug antiporter [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MYNRIAGYKQYYKSLVMLGVPIIIGQLGAIITGLADTIMVGQHSTEELAAASFVNNVINAFIIMGTGFSYNLTPLIGQNL
AMNKKIVIGGWLKNSLVANMTTTLLLIAVLLGIYLNLDSLGQPEELLPLIRPYFAVSLVSIIFVMLYNSFRQFVEGIANP
SISMWVLLTGNLLNIIGNYILIYGKCGMPELGLLGAGISTFISRIIMLLLFIAVFMLRTDYKPYRKGFTHIRINKQSWRR
LNSIGWPISLQQGLEAGTFCVTAIMVGWLGSLELAAHQIAITISTISFTIYLGLGAAVAIRTSYYKGANDWYMVRKITIA
GLHLGLIVVCIVCTTLFMTQSWLGLIFTDNLHVNAIVKTLLPILMLYQIGDSAQIILANSLRGLADVKAIMWISFLAYFI
IAIPAGYLLGFTFHWGIAGIWMAYPLGFLCSVCLLGYRAKMQVKSHLA

Sequences:

>Translated_448_residues
MYNRIAGYKQYYKSLVMLGVPIIIGQLGAIITGLADTIMVGQHSTEELAAASFVNNVINAFIIMGTGFSYNLTPLIGQNL
AMNKKIVIGGWLKNSLVANMTTTLLLIAVLLGIYLNLDSLGQPEELLPLIRPYFAVSLVSIIFVMLYNSFRQFVEGIANP
SISMWVLLTGNLLNIIGNYILIYGKCGMPELGLLGAGISTFISRIIMLLLFIAVFMLRTDYKPYRKGFTHIRINKQSWRR
LNSIGWPISLQQGLEAGTFCVTAIMVGWLGSLELAAHQIAITISTISFTIYLGLGAAVAIRTSYYKGANDWYMVRKITIA
GLHLGLIVVCIVCTTLFMTQSWLGLIFTDNLHVNAIVKTLLPILMLYQIGDSAQIILANSLRGLADVKAIMWISFLAYFI
IAIPAGYLLGFTFHWGIAGIWMAYPLGFLCSVCLLGYRAKMQVKSHLA
>Mature_448_residues
MYNRIAGYKQYYKSLVMLGVPIIIGQLGAIITGLADTIMVGQHSTEELAAASFVNNVINAFIIMGTGFSYNLTPLIGQNL
AMNKKIVIGGWLKNSLVANMTTTLLLIAVLLGIYLNLDSLGQPEELLPLIRPYFAVSLVSIIFVMLYNSFRQFVEGIANP
SISMWVLLTGNLLNIIGNYILIYGKCGMPELGLLGAGISTFISRIIMLLLFIAVFMLRTDYKPYRKGFTHIRINKQSWRR
LNSIGWPISLQQGLEAGTFCVTAIMVGWLGSLELAAHQIAITISTISFTIYLGLGAAVAIRTSYYKGANDWYMVRKITIA
GLHLGLIVVCIVCTTLFMTQSWLGLIFTDNLHVNAIVKTLLPILMLYQIGDSAQIILANSLRGLADVKAIMWISFLAYFI
IAIPAGYLLGFTFHWGIAGIWMAYPLGFLCSVCLLGYRAKMQVKSHLA

Specific function: Multidrug efflux pump that functions as a Na(+)/drug antiporter. Confers resistance to norfloxacin, ciprofloxacin, ofloxacin, daunomycin, doxorubicin, streptomycin, kanamycin, ethidium bromide and acriflavine [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI153792564, Length=444, Percent_Identity=22.2972972972973, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI22907060, Length=457, Percent_Identity=21.6630196936543, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI38261960, Length=277, Percent_Identity=24.187725631769, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI48994890, Length=448, Percent_Identity=28.125, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI226510954, Length=435, Percent_Identity=24.5977011494253, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49411; Mature: 49411

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNRIAGYKQYYKSLVMLGVPIIIGQLGAIITGLADTIMVGQHSTEELAAASFVNNVINA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
FIIMGTGFSYNLTPLIGQNLAMNKKIVIGGWLKNSLVANMTTTLLLIAVLLGIYLNLDSL
HEEEECCCCCCCCHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GQPEELLPLIRPYFAVSLVSIIFVMLYNSFRQFVEGIANPSISMWVLLTGNLLNIIGNYI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCEE
LIYGKCGMPELGLLGAGISTFISRIIMLLLFIAVFMLRTDYKPYRKGFTHIRINKQSWRR
EEEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEECHHHHHH
LNSIGWPISLQQGLEAGTFCVTAIMVGWLGSLELAAHQIAITISTISFTIYLGLGAAVAI
HHHCCCCEEHHHCCCHHHHHHHHHHHHHCCCHHHHHHEEEEEEEHHHEEHHHHCCHHHHH
RTSYYKGANDWYMVRKITIAGLHLGLIVVCIVCTTLFMTQSWLGLIFTDNLHVNAIVKTL
HHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHH
LPILMLYQIGDSAQIILANSLRGLADVKAIMWISFLAYFIIAIPAGYLLGFTFHWGIAGI
HHHHHHHHCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WMAYPLGFLCSVCLLGYRAKMQVKSHLA
HHHHHHHHHHHHHHHCHHHHHHHHHCCC
>Mature Secondary Structure
MYNRIAGYKQYYKSLVMLGVPIIIGQLGAIITGLADTIMVGQHSTEELAAASFVNNVINA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
FIIMGTGFSYNLTPLIGQNLAMNKKIVIGGWLKNSLVANMTTTLLLIAVLLGIYLNLDSL
HEEEECCCCCCCCHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GQPEELLPLIRPYFAVSLVSIIFVMLYNSFRQFVEGIANPSISMWVLLTGNLLNIIGNYI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCEE
LIYGKCGMPELGLLGAGISTFISRIIMLLLFIAVFMLRTDYKPYRKGFTHIRINKQSWRR
EEEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEECHHHHHH
LNSIGWPISLQQGLEAGTFCVTAIMVGWLGSLELAAHQIAITISTISFTIYLGLGAAVAI
HHHCCCCEEHHHCCCHHHHHHHHHHHHHCCCHHHHHHEEEEEEEHHHEEHHHHCCHHHHH
RTSYYKGANDWYMVRKITIAGLHLGLIVVCIVCTTLFMTQSWLGLIFTDNLHVNAIVKTL
HHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHH
LPILMLYQIGDSAQIILANSLRGLADVKAIMWISFLAYFIIAIPAGYLLGFTFHWGIAGI
HHHHHHHHCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WMAYPLGFLCSVCLLGYRAKMQVKSHLA
HHHHHHHHHHHHHHHCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11583854; 10952301 [H]