Click here to switch to the map view.

The map label for this gene is glyA

Identifier: 150007910

GI number: 150007910

Start: 1475627

End: 1476907

Strand: Direct

Name: glyA

Synonym: BDI_1271

Alternate gene names: 150007910

Gene position: 1475627-1476907 (Clockwise)

Preceding gene: 150007909

Following gene: 150007911

Centisome position: 30.67

GC content: 54.96

Gene sequence:

>1281_bases
ATGAAAAGAGACAACATCATTTTTGACATTATCGAGAAGGAGCACCAACGCCAATTGAAAGGCATTGAGTTGATCGCTTC
CGAGAACTTTGTCAGCGACCAGGTCATGCAGGCCATGGGAAGCTGTCTGACCAACAAATACGCCGAGGGATATCCCGGCA
AGCGTTATTATGGGGGTTGCGAGGTAGTAGACCAAAGTGAGACGATCGCTATCGAACGGTTGAAAAAGCTATTCAACGCA
GAATGGGCCAACGTGCAGCCTCACTCCGGGGCGCAGGCCAACGCAGCCGTATTCTTCGCCGTATTGAATCCGGGCGACAC
GTTCCTCGGGTTGAACCTGTCGCATGGCGGACACCTGTCCCACGGTTCACCAGTAAATAGCTCCGGCGTTCTCTATCACG
CTACCGAATATAATGTAAAGGAAGATACCGGACGGGTGGACTACGACCAGATGGAGGAAGTAGCCCTTCGTGAGAAACCG
AAATTGATCGTTGGCGGTGGTTCCGCTTATTCCCGTGACTGGGATTACAAACGTATGCGTGAGATAGCCGACAAGGTAGG
CGCCTTATTGATGATCGATATGGCCCACCCGGCAGGCCTGATCGCCGCCGGTTTGCTGAACAACCCGCTGGAGTACGCTC
ACATCGTAACGTCTACGACGCATAAGACCCTGCGTGGTCCTCGTGGCGGTATCATCCTGCTGGGTAAGGATTTCGAGAAT
CCTTGGGGCAAGAAGACACCGAAAGGCGAGATCAAGAAGATGTCTCAGTTGTTGGACTCCGCCGTATTCCCGGGTATCCA
AGGCGGTCCGCTGGAGCATGTGATCGCAGCCAAGGCCGTAGCCTTCGGAGAGGCTTTGGAGCCGGAATACAAGACGTACC
AAGCACAGGTGAAAGCGAACGCCGCCGCTATGGCCAAGGCCTTCACGGACAAAGGGTACAAGATCATCTCCGGCGGTACG
GACAACCACAGCATGCTGATCGACCTGCGCACGAAGTTCCCCGACCTGACCGGTAAAGTGGCCGAGAAAGCCTTGGTAGC
CGCCGACATCACGGTGAACAAGAACATGGTTCCGTTCGACAGCCGCTCGGCCTTCCAGACCTCCGGTATCCGTGTGGGTA
CGCCCGCTATCACGACCCGTGGCGCTAAAGAGCCGCTTATGGGCGAGATCGTGGAACTGATCGACACCGTATTGGCCGCT
CCCGAATGCGATAAGACCATCGGTGCCGTTCGTGAGAAGGTGAACGGTATCATGAAGGAATATCCTATCTTCGCATGGTA
A

Upstream 100 bases:

>100_bases
TAAACGGAATCTTTTAAATAGTATTAAAATAATATAGTACATTCGCGAGGATTCCTTTTCAATGAGGAACATATCATTGC
AATAACCAATTTAAGAGGTA

Downstream 100 bases:

>100_bases
ATAGGAAATTGAGAATTGAGAATTGAAAAGGATGCCAAACGGCATCTTATCTTCAACTCTCAATTCTCTTTTTAAACTCT
CAAAAACATCTAAACAATAT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKRDNIIFDIIEKEHQRQLKGIELIASENFVSDQVMQAMGSCLTNKYAEGYPGKRYYGGCEVVDQSETIAIERLKKLFNA
EWANVQPHSGAQANAAVFFAVLNPGDTFLGLNLSHGGHLSHGSPVNSSGVLYHATEYNVKEDTGRVDYDQMEEVALREKP
KLIVGGGSAYSRDWDYKRMREIADKVGALLMIDMAHPAGLIAAGLLNNPLEYAHIVTSTTHKTLRGPRGGIILLGKDFEN
PWGKKTPKGEIKKMSQLLDSAVFPGIQGGPLEHVIAAKAVAFGEALEPEYKTYQAQVKANAAAMAKAFTDKGYKIISGGT
DNHSMLIDLRTKFPDLTGKVAEKALVAADITVNKNMVPFDSRSAFQTSGIRVGTPAITTRGAKEPLMGEIVELIDTVLAA
PECDKTIGAVREKVNGIMKEYPIFAW

Sequences:

>Translated_426_residues
MKRDNIIFDIIEKEHQRQLKGIELIASENFVSDQVMQAMGSCLTNKYAEGYPGKRYYGGCEVVDQSETIAIERLKKLFNA
EWANVQPHSGAQANAAVFFAVLNPGDTFLGLNLSHGGHLSHGSPVNSSGVLYHATEYNVKEDTGRVDYDQMEEVALREKP
KLIVGGGSAYSRDWDYKRMREIADKVGALLMIDMAHPAGLIAAGLLNNPLEYAHIVTSTTHKTLRGPRGGIILLGKDFEN
PWGKKTPKGEIKKMSQLLDSAVFPGIQGGPLEHVIAAKAVAFGEALEPEYKTYQAQVKANAAAMAKAFTDKGYKIISGGT
DNHSMLIDLRTKFPDLTGKVAEKALVAADITVNKNMVPFDSRSAFQTSGIRVGTPAITTRGAKEPLMGEIVELIDTVLAA
PECDKTIGAVREKVNGIMKEYPIFAW
>Mature_426_residues
MKRDNIIFDIIEKEHQRQLKGIELIASENFVSDQVMQAMGSCLTNKYAEGYPGKRYYGGCEVVDQSETIAIERLKKLFNA
EWANVQPHSGAQANAAVFFAVLNPGDTFLGLNLSHGGHLSHGSPVNSSGVLYHATEYNVKEDTGRVDYDQMEEVALREKP
KLIVGGGSAYSRDWDYKRMREIADKVGALLMIDMAHPAGLIAAGLLNNPLEYAHIVTSTTHKTLRGPRGGIILLGKDFEN
PWGKKTPKGEIKKMSQLLDSAVFPGIQGGPLEHVIAAKAVAFGEALEPEYKTYQAQVKANAAAMAKAFTDKGYKIISGGT
DNHSMLIDLRTKFPDLTGKVAEKALVAADITVNKNMVPFDSRSAFQTSGIRVGTPAITTRGAKEPLMGEIVELIDTVLAA
PECDKTIGAVREKVNGIMKEYPIFAW

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI22547186, Length=397, Percent_Identity=48.3627204030227, Blast_Score=352, Evalue=3e-97,
Organism=Homo sapiens, GI19923315, Length=453, Percent_Identity=41.7218543046358, Blast_Score=351, Evalue=9e-97,
Organism=Homo sapiens, GI261862352, Length=453, Percent_Identity=41.7218543046358, Blast_Score=351, Evalue=9e-97,
Organism=Homo sapiens, GI261862350, Length=453, Percent_Identity=41.7218543046358, Blast_Score=351, Evalue=9e-97,
Organism=Homo sapiens, GI261862348, Length=453, Percent_Identity=41.7218543046358, Blast_Score=351, Evalue=9e-97,
Organism=Homo sapiens, GI261862346, Length=452, Percent_Identity=40.7079646017699, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI22547189, Length=397, Percent_Identity=44.3324937027708, Blast_Score=307, Evalue=1e-83,
Organism=Escherichia coli, GI1788902, Length=422, Percent_Identity=56.8720379146919, Blast_Score=436, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI25144732, Length=402, Percent_Identity=45.0248756218905, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI25144729, Length=402, Percent_Identity=45.0248756218905, Blast_Score=335, Evalue=3e-92,
Organism=Saccharomyces cerevisiae, GI6319739, Length=416, Percent_Identity=44.4711538461538, Blast_Score=342, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6323087, Length=397, Percent_Identity=46.3476070528967, Blast_Score=335, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24640005, Length=404, Percent_Identity=46.5346534653465, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI221329721, Length=404, Percent_Identity=46.2871287128713, Blast_Score=362, Evalue=1e-100,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_PARD8 (A6LBG7)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001302653.1
- ProteinModelPortal:   A6LBG7
- SMR:   A6LBG7
- STRING:   A6LBG7
- GeneID:   5306424
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_1271
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   KAMARTF
- ProtClustDB:   PRK00011
- BioCyc:   PDIS435591:BDI_1271-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 46440; Mature: 46440

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRDNIIFDIIEKEHQRQLKGIELIASENFVSDQVMQAMGSCLTNKYAEGYPGKRYYGGC
CCCCCEEHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCC
EVVDQSETIAIERLKKLFNAEWANVQPHSGAQANAAVFFAVLNPGDTFLGLNLSHGGHLS
EEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCC
HGSPVNSSGVLYHATEYNVKEDTGRVDYDQMEEVALREKPKLIVGGGSAYSRDWDYKRMR
CCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHH
EIADKVGALLMIDMAHPAGLIAAGLLNNPLEYAHIVTSTTHKTLRGPRGGIILLGKDFEN
HHHHHCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHCHHHHHCCCCCCEEEEECCCCC
PWGKKTPKGEIKKMSQLLDSAVFPGIQGGPLEHVIAAKAVAFGEALEPEYKTYQAQVKAN
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AAAMAKAFTDKGYKIISGGTDNHSMLIDLRTKFPDLTGKVAEKALVAADITVNKNMVPFD
HHHHHHHHHCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHEEEEEEECCCCCCCC
SRSAFQTSGIRVGTPAITTRGAKEPLMGEIVELIDTVLAAPECDKTIGAVREKVNGIMKE
CCCCHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
YPIFAW
CCCCCC
>Mature Secondary Structure
MKRDNIIFDIIEKEHQRQLKGIELIASENFVSDQVMQAMGSCLTNKYAEGYPGKRYYGGC
CCCCCEEHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCC
EVVDQSETIAIERLKKLFNAEWANVQPHSGAQANAAVFFAVLNPGDTFLGLNLSHGGHLS
EEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCC
HGSPVNSSGVLYHATEYNVKEDTGRVDYDQMEEVALREKPKLIVGGGSAYSRDWDYKRMR
CCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHH
EIADKVGALLMIDMAHPAGLIAAGLLNNPLEYAHIVTSTTHKTLRGPRGGIILLGKDFEN
HHHHHCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHCHHHHHCCCCCCEEEEECCCCC
PWGKKTPKGEIKKMSQLLDSAVFPGIQGGPLEHVIAAKAVAFGEALEPEYKTYQAQVKAN
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AAAMAKAFTDKGYKIISGGTDNHSMLIDLRTKFPDLTGKVAEKALVAADITVNKNMVPFD
HHHHHHHHHCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHEEEEEEECCCCCCCC
SRSAFQTSGIRVGTPAITTRGAKEPLMGEIVELIDTVLAAPECDKTIGAVREKVNGIMKE
CCCCHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
YPIFAW
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA