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The map label for this gene is trmD [H]

Identifier: 150007875

GI number: 150007875

Start: 1433642

End: 1434373

Strand: Direct

Name: trmD [H]

Synonym: BDI_1235

Alternate gene names: 150007875

Gene position: 1433642-1434373 (Clockwise)

Preceding gene: 150007868

Following gene: 150007877

Centisome position: 29.8

GC content: 49.32

Gene sequence:

>732_bases
ATGAGTCAGAATAAAGATGTACCTTTGCGGGAGGTTTTAACGTTACAACTATTTTTTATGCGTATAGATATACTTACCGT
ACTTCCCGAGATGATCGAGGGGATGATTAATTGCTCTATCGTCAAGAGGGCGCAAGATAAAGGATTAGCGGAGATTCATT
TACATAACCTACGGGATTACACCACTAATAAATGGAGGCGGGTGGATGATTATCCGTTCGGAGGAGAGGCCGGAATGGTG
ATGCAGATCGAACCGATAGACCGGGCGATCTCAGCGTTGAAAAGCGAACGGGAATATGACGAGGTAATCTATACCTCCCC
GGATGGCGAGACACTCAACCAGCCCATGGCCAACAGCATGTCTTTACTAAATAATATGATTATCCTCTGCGGGCACTATA
AAGGGATCGACTATCGTATCCGTGAGCATTTGATAACGAAAGAGATATCCGTAGGAGACTATGTCCTTACCGGAGGAGAG
CTGGCCGCCGCTATCATAACCGACGCCGTGGTACGCTTGATACCGGGAGCGATCGGCGACGAGCAAAGCGCCCTCTCCGA
CTCCTTCCAAGATGACTTACTGGCCCCGCCTGTTTATACACGTCCCGCCGATTACAAAGGTTGGAAAGTACCCGAGGTAC
TATTATCCGGGCACCAGCGCAAGATCGAAGAATGGCGCTTGCAACAAGCACAAGAGCGTACGGCACGCCTCCGGCCGGAT
TTATTGAAATAG

Upstream 100 bases:

>100_bases
CCTCATGAACTTCTACGAACTCTTAACTGCGCCAACGCATGCGTTTCATTCATTCTTAATTTTCTATCGCGAATCATAGA
TAATAGAGACTCACTTTACG

Downstream 100 bases:

>100_bases
CAGCGTTAAAAAACAAGGCCGGGGATCATCCCCCCCGATGATACCCCGGCCCCTCTATCCACAAACAATCATGGCTACAA
CATCACTCTTATTTATTGAA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSQNKDVPLREVLTLQLFFMRIDILTVLPEMIEGMINCSIVKRAQDKGLAEIHLHNLRDYTTNKWRRVDDYPFGGEAGMV
MQIEPIDRAISALKSEREYDEVIYTSPDGETLNQPMANSMSLLNNMIILCGHYKGIDYRIREHLITKEISVGDYVLTGGE
LAAAIITDAVVRLIPGAIGDEQSALSDSFQDDLLAPPVYTRPADYKGWKVPEVLLSGHQRKIEEWRLQQAQERTARLRPD
LLK

Sequences:

>Translated_243_residues
MSQNKDVPLREVLTLQLFFMRIDILTVLPEMIEGMINCSIVKRAQDKGLAEIHLHNLRDYTTNKWRRVDDYPFGGEAGMV
MQIEPIDRAISALKSEREYDEVIYTSPDGETLNQPMANSMSLLNNMIILCGHYKGIDYRIREHLITKEISVGDYVLTGGE
LAAAIITDAVVRLIPGAIGDEQSALSDSFQDDLLAPPVYTRPADYKGWKVPEVLLSGHQRKIEEWRLQQAQERTARLRPD
LLK
>Mature_242_residues
SQNKDVPLREVLTLQLFFMRIDILTVLPEMIEGMINCSIVKRAQDKGLAEIHLHNLRDYTTNKWRRVDDYPFGGEAGMVM
QIEPIDRAISALKSEREYDEVIYTSPDGETLNQPMANSMSLLNNMIILCGHYKGIDYRIREHLITKEISVGDYVLTGGEL
AAAIITDAVVRLIPGAIGDEQSALSDSFQDDLLAPPVYTRPADYKGWKVPEVLLSGHQRKIEEWRLQQAQERTARLRPDL
LK

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=225, Percent_Identity=42.6666666666667, Blast_Score=179, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27625; Mature: 27494

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNKDVPLREVLTLQLFFMRIDILTVLPEMIEGMINCSIVKRAQDKGLAEIHLHNLRDY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TTNKWRRVDDYPFGGEAGMVMQIEPIDRAISALKSEREYDEVIYTSPDGETLNQPMANSM
HCCCCEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHH
SLLNNMIILCGHYKGIDYRIREHLITKEISVGDYVLTGGELAAAIITDAVVRLIPGAIGD
HHHHCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCC
EQSALSDSFQDDLLAPPVYTRPADYKGWKVPEVLLSGHQRKIEEWRLQQAQERTARLRPD
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
LLK
CCC
>Mature Secondary Structure 
SQNKDVPLREVLTLQLFFMRIDILTVLPEMIEGMINCSIVKRAQDKGLAEIHLHNLRDY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TTNKWRRVDDYPFGGEAGMVMQIEPIDRAISALKSEREYDEVIYTSPDGETLNQPMANSM
HCCCCEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHH
SLLNNMIILCGHYKGIDYRIREHLITKEISVGDYVLTGGELAAAIITDAVVRLIPGAIGD
HHHHCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCC
EQSALSDSFQDDLLAPPVYTRPADYKGWKVPEVLLSGHQRKIEEWRLQQAQERTARLRPD
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
LLK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA