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The map label for this gene is nfo

Identifier: 150007842

GI number: 150007842

Start: 1396277

End: 1397122

Strand: Reverse

Name: nfo

Synonym: BDI_1200

Alternate gene names: 150007842

Gene position: 1397122-1396277 (Counterclockwise)

Preceding gene: 150007843

Following gene: 150007841

Centisome position: 29.04

GC content: 51.89

Gene sequence:

>846_bases
ATGAAGTATGTTGGGGCTCACGTGAGTGCGGTCGGTGGGGTTGAGAATGCCCCCGTTAATGCCCATGAGATCGGTGCGAA
GGCTTTTGCCCTTTTCACCCGGAATCAGAGGCAATGGAAGTCGCAGCCGTTGAAGGCGGACAGTATCCATTTATTTAAGG
AGCGGTGTGAAGCGTACGGTTACGATCCGGGATGTATCTTGCCGCACGATAGCTATTTGATCAATCTGGGGAACCCGGAC
GCCGAGGGATTGCAGAAATCCCGTGACGCTTTTCTGGACGAGATGACACGCTGCGAGCAACTGGGACTGAAAATGCTGAA
TTTCCATCCCGGAAGCCATTTGGGGAAGATGGAGGTGGATACCTGCCTGTCTCGCATCGCCGAGTCGATCAATATCACGT
TGGCTCAGACGAGTGGCGTATGTGCCGTGATAGAGAATACGGCGGGGCAAGGCTCGAACTTGGGATATACCTTCGAGCAG
ATCGCCTATATTATAAATGAGGTAGAGGATAAGAGCCGGGTGGGAGTATGCCTCGATACGGCGCACACGCTTGCCGCCGG
TTACGATATAAAGACACCGGAGGGATTTGCCGAGACATTTCGTCATTTCGACGAGGTGGTCGGCTTTTCTTATTTAAGGG
GTATGCACTTGAACGATTCTAAAAAGGAGCTGGGATCACGTGTGGACCGGCATGAGAGTATCGGTAAAGGCTTGATGGGC
CTTGATACCTTCCGCATGATCATGGTGGACCCCCGTTTCGACAATATGCCCTTAATCCTAGAGACCCCGGACGAGGCGCT
TTGGCCGGAGGAGATACAGCTTCTATATAAACAATTAAAATCTTGA

Upstream 100 bases:

>100_bases
TTTCATCACCCCGCAAGAACGTGCGATGGAGGAGTTTGGTTTTTGTGGGTGTAAGACTGAGGATGAAATTAGTATAAATA
AAAAGAAAAGTTAGGTTATT

Downstream 100 bases:

>100_bases
TTATATGTTCATTTTAATGCCTGTCATTTTTGTCTTAGGAATCCTCGCTATTGCCTTGGAGGATAAGATCAAAATAAATA
AAGCGGCCATAGCGTTGTTC

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MKYVGAHVSAVGGVENAPVNAHEIGAKAFALFTRNQRQWKSQPLKADSIHLFKERCEAYGYDPGCILPHDSYLINLGNPD
AEGLQKSRDAFLDEMTRCEQLGLKMLNFHPGSHLGKMEVDTCLSRIAESINITLAQTSGVCAVIENTAGQGSNLGYTFEQ
IAYIINEVEDKSRVGVCLDTAHTLAAGYDIKTPEGFAETFRHFDEVVGFSYLRGMHLNDSKKELGSRVDRHESIGKGLMG
LDTFRMIMVDPRFDNMPLILETPDEALWPEEIQLLYKQLKS

Sequences:

>Translated_281_residues
MKYVGAHVSAVGGVENAPVNAHEIGAKAFALFTRNQRQWKSQPLKADSIHLFKERCEAYGYDPGCILPHDSYLINLGNPD
AEGLQKSRDAFLDEMTRCEQLGLKMLNFHPGSHLGKMEVDTCLSRIAESINITLAQTSGVCAVIENTAGQGSNLGYTFEQ
IAYIINEVEDKSRVGVCLDTAHTLAAGYDIKTPEGFAETFRHFDEVVGFSYLRGMHLNDSKKELGSRVDRHESIGKGLMG
LDTFRMIMVDPRFDNMPLILETPDEALWPEEIQLLYKQLKS
>Mature_281_residues
MKYVGAHVSAVGGVENAPVNAHEIGAKAFALFTRNQRQWKSQPLKADSIHLFKERCEAYGYDPGCILPHDSYLINLGNPD
AEGLQKSRDAFLDEMTRCEQLGLKMLNFHPGSHLGKMEVDTCLSRIAESINITLAQTSGVCAVIENTAGQGSNLGYTFEQ
IAYIINEVEDKSRVGVCLDTAHTLAAGYDIKTPEGFAETFRHFDEVVGFSYLRGMHLNDSKKELGSRVDRHESIGKGLMG
LDTFRMIMVDPRFDNMPLILETPDEALWPEEIQLLYKQLKS

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=278, Percent_Identity=64.0287769784173, Blast_Score=374, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17531193, Length=275, Percent_Identity=46.9090909090909, Blast_Score=283, Evalue=9e-77,
Organism=Saccharomyces cerevisiae, GI6322735, Length=274, Percent_Identity=43.0656934306569, Blast_Score=244, Evalue=8e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_PARD8 (A6LB99)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001302585.1
- ProteinModelPortal:   A6LB99
- SMR:   A6LB99
- STRING:   A6LB99
- GeneID:   5306353
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_1200
- eggNOG:   COG0648
- HOGENOM:   HBG565018
- OMA:   QIALETM
- BioCyc:   PDIS435591:BDI_1200-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 31301; Mature: 31301

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYVGAHVSAVGGVENAPVNAHEIGAKAFALFTRNQRQWKSQPLKADSIHLFKERCEAYG
CCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHCC
YDPGCILPHDSYLINLGNPDAEGLQKSRDAFLDEMTRCEQLGLKMLNFHPGSHLGKMEVD
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCHHHH
TCLSRIAESINITLAQTSGVCAVIENTAGQGSNLGYTFEQIAYIINEVEDKSRVGVCLDT
HHHHHHHHHHEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHH
AHTLAAGYDIKTPEGFAETFRHFDEVVGFSYLRGMHLNDSKKELGSRVDRHESIGKGLMG
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHH
LDTFRMIMVDPRFDNMPLILETPDEALWPEEIQLLYKQLKS
HHHEEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKYVGAHVSAVGGVENAPVNAHEIGAKAFALFTRNQRQWKSQPLKADSIHLFKERCEAYG
CCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHCC
YDPGCILPHDSYLINLGNPDAEGLQKSRDAFLDEMTRCEQLGLKMLNFHPGSHLGKMEVD
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCHHHH
TCLSRIAESINITLAQTSGVCAVIENTAGQGSNLGYTFEQIAYIINEVEDKSRVGVCLDT
HHHHHHHHHHEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHH
AHTLAAGYDIKTPEGFAETFRHFDEVVGFSYLRGMHLNDSKKELGSRVDRHESIGKGLMG
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHH
LDTFRMIMVDPRFDNMPLILETPDEALWPEEIQLLYKQLKS
HHHEEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA