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The map label for this gene is 150007817

Identifier: 150007817

GI number: 150007817

Start: 1369406

End: 1370281

Strand: Reverse

Name: 150007817

Synonym: BDI_1175

Alternate gene names: NA

Gene position: 1370281-1369406 (Counterclockwise)

Preceding gene: 150007818

Following gene: 150007816

Centisome position: 28.48

GC content: 53.31

Gene sequence:

>876_bases
ATGAAAGTAGGATTAATACAACAAAAGAATACCGCTGACAGAGCGGCGAATATAGAGAAGCTAAAGGTAAATATCCGTAA
GGCTGCCCGGGAGGGAGCGGAATTGGTCGTCTTGCAAGAACTGCATAACGGCCTGTATTTCTGCCAGACGGAAGATACGA
GTATGTTTGACTTGGCAGAGACGATCCCCGGCCCATCGACGGAAACCTTCGGTGCCTTGGCGAAAGAATTGGGTATCGTC
TTGGTACTTTCCCTTTTCGAGAAGCGTGCCCCGGGCCTGTACCATAACACGGCCGTCGTCTTGGAGAAAGACGGTACGAT
CGCCGGAAAATACCGCAAGATGCATATCCCGGATGACCCCGCTTATTACGAGAAGTTCTATTTTACCCCCGGTGATTTAG
GCTTTGAGCCTATCGATACCTCTGTGGGGCGTCTTGGTGTGTTGGTTTGCTGGGATCAATGGTATCCGGAAGCAGCCCGT
TTGATGGCTATGCGTGGCGCCGAGATGTTGATCTATCCGACCGCTATCGGCTGGGAAAGTAGCGATACGCAGGAAGAGAA
GGACCGTCAGCTGGGTGCGTGGGTGACCATACAGCGGGGACATGCCGTGGCGAACGGACTCCCGGTCATTTCCGTAAATC
GTACCGGGCATGAGCCGGACCCTTCGGGACAAACGGGCGGTATCCGGTTTTGGGGAAACAGTTTCGCGGCCGGTCCCCAA
GGTGAGCTATTAACCGTATTCCCGAATGACGAGGAGGAGGTACGTGTGATAGAGATCGACAAGACTCGCGGTGAGAACGT
GCGTCGTTGGTGGCCTTTCTTCCGGGATCGCCGTATCGATGCGTTTGGTGGTTTGACAGAACGATTTTTAGTATAA

Upstream 100 bases:

>100_bases
ATCAATTGCCTTCCGCTTATCAAGCAACACGGTTCCCTGCATTGCGTAACGATGCAGTATCCGGAAGGTTTTATCGAATA
ACAACTACAACCAATTAAAT

Downstream 100 bases:

>100_bases
TGACCGAATACACGCAAGAAGAGGCCATCTTCCGCATGAACGAAATGGGGAAAGCGGGACGGCCTTTCCTTTTTATCATT
GATTATAAACGAGAGCGGAT

Product: beta-ureidopropionase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MKVGLIQQKNTADRAANIEKLKVNIRKAAREGAELVVLQELHNGLYFCQTEDTSMFDLAETIPGPSTETFGALAKELGIV
LVLSLFEKRAPGLYHNTAVVLEKDGTIAGKYRKMHIPDDPAYYEKFYFTPGDLGFEPIDTSVGRLGVLVCWDQWYPEAAR
LMAMRGAEMLIYPTAIGWESSDTQEEKDRQLGAWVTIQRGHAVANGLPVISVNRTGHEPDPSGQTGGIRFWGNSFAAGPQ
GELLTVFPNDEEEVRVIEIDKTRGENVRRWWPFFRDRRIDAFGGLTERFLV

Sequences:

>Translated_291_residues
MKVGLIQQKNTADRAANIEKLKVNIRKAAREGAELVVLQELHNGLYFCQTEDTSMFDLAETIPGPSTETFGALAKELGIV
LVLSLFEKRAPGLYHNTAVVLEKDGTIAGKYRKMHIPDDPAYYEKFYFTPGDLGFEPIDTSVGRLGVLVCWDQWYPEAAR
LMAMRGAEMLIYPTAIGWESSDTQEEKDRQLGAWVTIQRGHAVANGLPVISVNRTGHEPDPSGQTGGIRFWGNSFAAGPQ
GELLTVFPNDEEEVRVIEIDKTRGENVRRWWPFFRDRRIDAFGGLTERFLV
>Mature_291_residues
MKVGLIQQKNTADRAANIEKLKVNIRKAAREGAELVVLQELHNGLYFCQTEDTSMFDLAETIPGPSTETFGALAKELGIV
LVLSLFEKRAPGLYHNTAVVLEKDGTIAGKYRKMHIPDDPAYYEKFYFTPGDLGFEPIDTSVGRLGVLVCWDQWYPEAAR
LMAMRGAEMLIYPTAIGWESSDTQEEKDRQLGAWVTIQRGHAVANGLPVISVNRTGHEPDPSGQTGGIRFWGNSFAAGPQ
GELLTVFPNDEEEVRVIEIDKTRGENVRRWWPFFRDRRIDAFGGLTERFLV

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=288, Percent_Identity=29.5138888888889, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI297632348, Length=296, Percent_Identity=28.0405405405405, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI297632350, Length=296, Percent_Identity=28.0405405405405, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI5031947, Length=296, Percent_Identity=28.0405405405405, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI7706509, Length=304, Percent_Identity=25.6578947368421, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI297632346, Length=185, Percent_Identity=31.3513513513514, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17533173, Length=283, Percent_Identity=27.5618374558304, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17556280, Length=288, Percent_Identity=25.6944444444444, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6323383, Length=302, Percent_Identity=27.4834437086093, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322335, Length=315, Percent_Identity=22.5396825396825, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21358471, Length=307, Percent_Identity=28.0130293159609, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17933642, Length=282, Percent_Identity=25.177304964539, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355835, Length=297, Percent_Identity=27.9461279461279, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 32634; Mature: 32634

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGLIQQKNTADRAANIEKLKVNIRKAAREGAELVVLQELHNGLYFCQTEDTSMFDLAE
CCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEEEHHHCCEEEEEECCCCHHHHHH
TIPGPSTETFGALAKELGIVLVLSLFEKRAPGLYHNTAVVLEKDGTIAGKYRKMHIPDDP
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEECEEEEECCCCC
AYYEKFYFTPGDLGFEPIDTSVGRLGVLVCWDQWYPEAARLMAMRGAEMLIYPTAIGWES
HHHHEEEECCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEEECCCC
SDTQEEKDRQLGAWVTIQRGHAVANGLPVISVNRTGHEPDPSGQTGGIRFWGNSFAAGPQ
CCCHHHHHHCCCEEEEEECCCCHHCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCC
GELLTVFPNDEEEVRVIEIDKTRGENVRRWWPFFRDRRIDAFGGLTERFLV
CCEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCHHHCCCHHHHCC
>Mature Secondary Structure
MKVGLIQQKNTADRAANIEKLKVNIRKAAREGAELVVLQELHNGLYFCQTEDTSMFDLAE
CCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEEEHHHCCEEEEEECCCCHHHHHH
TIPGPSTETFGALAKELGIVLVLSLFEKRAPGLYHNTAVVLEKDGTIAGKYRKMHIPDDP
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEECEEEEECCCCC
AYYEKFYFTPGDLGFEPIDTSVGRLGVLVCWDQWYPEAARLMAMRGAEMLIYPTAIGWES
HHHHEEEECCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEEECCCC
SDTQEEKDRQLGAWVTIQRGHAVANGLPVISVNRTGHEPDPSGQTGGIRFWGNSFAAGPQ
CCCHHHHHHCCCEEEEEECCCCHHCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCC
GELLTVFPNDEEEVRVIEIDKTRGENVRRWWPFFRDRRIDAFGGLTERFLV
CCEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]