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The map label for this gene is nth [H]

Identifier: 150007785

GI number: 150007785

Start: 1322001

End: 1322666

Strand: Direct

Name: nth [H]

Synonym: BDI_1143

Alternate gene names: 150007785

Gene position: 1322001-1322666 (Clockwise)

Preceding gene: 150007784

Following gene: 150007786

Centisome position: 27.48

GC content: 46.1

Gene sequence:

>666_bases
ATGCGAAAAACAGAAACCGAAAAAGCAATGCGAAAAGAAGAAAGGTATAAAGGCGTATTAAACTGGTTTAAGGAAAACGT
ACCGGTAGCCGAGACTGAACTACATTATGACGACCCCTATCAACTATTGATAGCGGTAATCCTATCCGCACAATGCACGG
ATAAGCGAGTGAATATGATCACACCTGCCCTGTTCGAGGCTTTCCCGACTCCCGAGGTAATGGCGGCTAGCACACCGGAA
GTTGTCTTTGAATACATACGAAGCGTATCTTACCCGAACAATAAGGCTAAACATCTGGTAGGTATGGCGAAAATGCTGAT
AGAGGATTTCAAAGGCGTGGTTCCCTCCGATATTGATGAGCTACAAAAACTGCCGGGCGTAGGAAGAAAAACAGCGAACG
TAATCGCATCCGTAGTATATGACAAGCCGGCCATGGCGGTGGATACGCACGTATTCCGTGTTTCCAACCGGATCGGCTTG
ACCAATAATAGCAAGACACCTTTGGAAACGGAAAAGGAGTTGGTGAAGAACATTCCGGAAGAATTGATCCCCATCGCCCA
TCACTGGTTGATATTGCATGGAAGATATGTTTGCCTCGCCCGGAAACCGAAATGTGAGGAATGCGGATTAAAGCCTTGGT
GTAAACACTTCCTAAAGCAAACATGA

Upstream 100 bases:

>100_bases
AAGGAGATCGGTACTTTTTTATTTATTCGTACAATTCGGGTGTATCTTATTTTTGGCACATCTCCGCTTGTATATTCCTA
TTAAAAGTTATACCTTTAGG

Downstream 100 bases:

>100_bases
GGATCAGCAAGAAAGACATAACGGCATTTTTTGTCTTGTTTCTGGGTACGATCGTATGCGTGAGGTACTTTTACAAGCAT
ATGAACGACGAGCAATTTGT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MRKTETEKAMRKEERYKGVLNWFKENVPVAETELHYDDPYQLLIAVILSAQCTDKRVNMITPALFEAFPTPEVMAASTPE
VVFEYIRSVSYPNNKAKHLVGMAKMLIEDFKGVVPSDIDELQKLPGVGRKTANVIASVVYDKPAMAVDTHVFRVSNRIGL
TNNSKTPLETEKELVKNIPEELIPIAHHWLILHGRYVCLARKPKCEECGLKPWCKHFLKQT

Sequences:

>Translated_221_residues
MRKTETEKAMRKEERYKGVLNWFKENVPVAETELHYDDPYQLLIAVILSAQCTDKRVNMITPALFEAFPTPEVMAASTPE
VVFEYIRSVSYPNNKAKHLVGMAKMLIEDFKGVVPSDIDELQKLPGVGRKTANVIASVVYDKPAMAVDTHVFRVSNRIGL
TNNSKTPLETEKELVKNIPEELIPIAHHWLILHGRYVCLARKPKCEECGLKPWCKHFLKQT
>Mature_221_residues
MRKTETEKAMRKEERYKGVLNWFKENVPVAETELHYDDPYQLLIAVILSAQCTDKRVNMITPALFEAFPTPEVMAASTPE
VVFEYIRSVSYPNNKAKHLVGMAKMLIEDFKGVVPSDIDELQKLPGVGRKTANVIASVVYDKPAMAVDTHVFRVSNRIGL
TNNSKTPLETEKELVKNIPEELIPIAHHWLILHGRYVCLARKPKCEECGLKPWCKHFLKQT

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=196, Percent_Identity=29.5918367346939, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1787920, Length=207, Percent_Identity=42.9951690821256, Blast_Score=172, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17554540, Length=186, Percent_Identity=34.9462365591398, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324530, Length=181, Percent_Identity=28.7292817679558, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=31.0734463276836, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 25205; Mature: 25205

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTETEKAMRKEERYKGVLNWFKENVPVAETELHYDDPYQLLIAVILSAQCTDKRVNMI
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHH
TPALFEAFPTPEVMAASTPEVVFEYIRSVSYPNNKAKHLVGMAKMLIEDFKGVVPSDIDE
HHHHHHHCCCCHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
LQKLPGVGRKTANVIASVVYDKPAMAVDTHVFRVSNRIGLTNNSKTPLETEKELVKNIPE
HHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCHH
ELIPIAHHWLILHGRYVCLARKPKCEECGLKPWCKHFLKQT
HHHHHHHHHHHHHCCEEEEECCCCHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MRKTETEKAMRKEERYKGVLNWFKENVPVAETELHYDDPYQLLIAVILSAQCTDKRVNMI
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHH
TPALFEAFPTPEVMAASTPEVVFEYIRSVSYPNNKAKHLVGMAKMLIEDFKGVVPSDIDE
HHHHHHHCCCCHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
LQKLPGVGRKTANVIASVVYDKPAMAVDTHVFRVSNRIGLTNNSKTPLETEKELVKNIPE
HHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCHH
ELIPIAHHWLILHGRYVCLARKPKCEECGLKPWCKHFLKQT
HHHHHHHHHHHHHCCEEEEECCCCHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274032; 9823893 [H]