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The map label for this gene is menE [H]

Identifier: 150007781

GI number: 150007781

Start: 1314619

End: 1315686

Strand: Direct

Name: menE [H]

Synonym: BDI_1139

Alternate gene names: 150007781

Gene position: 1314619-1315686 (Clockwise)

Preceding gene: 150007780

Following gene: 150007782

Centisome position: 27.32

GC content: 57.58

Gene sequence:

>1068_bases
ATGCCGATACAAGAAGATTTACAGGCATTCTTAGCTGAATGGCACGATCCCTCCCCTTTTCTGGAAGTCCAGACCTCCGG
CTCCACCGGTACGCCCAAGCGGATGAGAGTGTGCAAGGACCGGATGCTGAACAGCGCCCGGCTTACCTGCGATTATCTGG
GTCTTAAGAAGGGCGACAAGGCCCTGTTATGTATGCCCCTGCGCTATATAGCCGGAAAGATGATGGTGGTCCGCTCCCTA
TACGCCGGACTGGATCTGGAGGTGCGTGAGCCCTCCGGACACCCGTTGGCGGATTGGGGGGATACGCCCCTGCGCTTTGC
CGCCATGATCCCCTTGCAGGTTTACAATACGTTGCGAGTACCCGAGGAACGTAAACGGCTGGAGCAAACGGATATCCTGA
TTATCGGCGGCGGGGCGATCGACGCGGCCTTGGAACAGGAGATACGGCAGATGCCAAACACGGTTTATTCCACCTACGGA
ATGACGGAGACGCTGTCTCACGTCGCCCTGCGCCGGCTGAACGGGCCGGAAGCCTCGCCCTATTACCATCCCTTCCCCTC
GGTGACGTTATCCTTGTCGCCGGACAATACCTTGGTGATAGACGCCCCATTGGTTTGCGACGAGCGGCTGGTGACGAACG
ATGTCGCCCGCCTGCTGCCGGACGGGAGTTTCGCCATTATCGGAAGGAAAGATAATATCATCAATAGCGGAGGTATCAAG
ATACAGATCGAGGAGGTGGAAGAGCGGCTACGGCCCTACCTAGACCTCCCCTATGCCATCACCGCCGCCCCCGACCCTCG
GTTGGGAGAGGCGGTAGTCTTACTAATCGCCCCAAGGTCATGTGAAACGGTATCACCCCCTCGGGTGACACCACACATGA
AAGCTATCATGAGCGACTCCGCGCAGCTATCATGTGAGGTATCGACAGTGGCTCTCGCCAGCCTGCCCAAGTACCTCCGT
CCAAGGCACGTCATAGAGGCAAGCGAGATCCCCCAAACCGGAAGCGGCAAGATCGATCGTGCCGCCTGCCGGGAGCTAGC
GAGAAATATCATCACAAAAACACTATAA

Upstream 100 bases:

>100_bases
ACCGCAAGGTCTGGGAACCGGCGAGATTTTCAGGGATGATAAACATACGTTGATCCAACGAAAAGGAGACACCATCTGGT
TCACTAACGACCTTTGACCT

Downstream 100 bases:

>100_bases
ACATGAAAAGGATTCTAACAATCACCGCGGTCTCCCTATTGGCCTTATCGCCTATGTACGGGCAGGATTCTTGCCGATAC
CCGCAAGATCCTACCTTGGA

Product: putative O-succinylbenzoate--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MPIQEDLQAFLAEWHDPSPFLEVQTSGSTGTPKRMRVCKDRMLNSARLTCDYLGLKKGDKALLCMPLRYIAGKMMVVRSL
YAGLDLEVREPSGHPLADWGDTPLRFAAMIPLQVYNTLRVPEERKRLEQTDILIIGGGAIDAALEQEIRQMPNTVYSTYG
MTETLSHVALRRLNGPEASPYYHPFPSVTLSLSPDNTLVIDAPLVCDERLVTNDVARLLPDGSFAIIGRKDNIINSGGIK
IQIEEVEERLRPYLDLPYAITAAPDPRLGEAVVLLIAPRSCETVSPPRVTPHMKAIMSDSAQLSCEVSTVALASLPKYLR
PRHVIEASEIPQTGSGKIDRAACRELARNIITKTL

Sequences:

>Translated_355_residues
MPIQEDLQAFLAEWHDPSPFLEVQTSGSTGTPKRMRVCKDRMLNSARLTCDYLGLKKGDKALLCMPLRYIAGKMMVVRSL
YAGLDLEVREPSGHPLADWGDTPLRFAAMIPLQVYNTLRVPEERKRLEQTDILIIGGGAIDAALEQEIRQMPNTVYSTYG
MTETLSHVALRRLNGPEASPYYHPFPSVTLSLSPDNTLVIDAPLVCDERLVTNDVARLLPDGSFAIIGRKDNIINSGGIK
IQIEEVEERLRPYLDLPYAITAAPDPRLGEAVVLLIAPRSCETVSPPRVTPHMKAIMSDSAQLSCEVSTVALASLPKYLR
PRHVIEASEIPQTGSGKIDRAACRELARNIITKTL
>Mature_354_residues
PIQEDLQAFLAEWHDPSPFLEVQTSGSTGTPKRMRVCKDRMLNSARLTCDYLGLKKGDKALLCMPLRYIAGKMMVVRSLY
AGLDLEVREPSGHPLADWGDTPLRFAAMIPLQVYNTLRVPEERKRLEQTDILIIGGGAIDAALEQEIRQMPNTVYSTYGM
TETLSHVALRRLNGPEASPYYHPFPSVTLSLSPDNTLVIDAPLVCDERLVTNDVARLLPDGSFAIIGRKDNIINSGGIKI
QIEEVEERLRPYLDLPYAITAAPDPRLGEAVVLLIAPRSCETVSPPRVTPHMKAIMSDSAQLSCEVSTVALASLPKYLRP
RHVIEASEIPQTGSGKIDRAACRELARNIITKTL

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=238, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 39238; Mature: 39107

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIQEDLQAFLAEWHDPSPFLEVQTSGSTGTPKRMRVCKDRMLNSARLTCDYLGLKKGDK
CCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHEEEEEECCCCCCC
ALLCMPLRYIAGKMMVVRSLYAGLDLEVREPSGHPLADWGDTPLRFAAMIPLQVYNTLRV
EEEEECHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEHHEECHHHHHHHCCC
PEERKRLEQTDILIIGGGAIDAALEQEIRQMPNTVYSTYGMTETLSHVALRRLNGPEASP
CHHHHHHHHCCEEEEECCHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHCCCCCCCC
YYHPFPSVTLSLSPDNTLVIDAPLVCDERLVTNDVARLLPDGSFAIIGRKDNIINSGGIK
CCCCCCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEECCCCEECCCCCE
IQIEEVEERLRPYLDLPYAITAAPDPRLGEAVVLLIAPRSCETVSPPRVTPHMKAIMSDS
EEHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCC
AQLSCEVSTVALASLPKYLRPRHVIEASEIPQTGSGKIDRAACRELARNIITKTL
CCEEEEHHHHHHHHHHHHHCCCCEECHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PIQEDLQAFLAEWHDPSPFLEVQTSGSTGTPKRMRVCKDRMLNSARLTCDYLGLKKGDK
CCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHEEEEEECCCCCCC
ALLCMPLRYIAGKMMVVRSLYAGLDLEVREPSGHPLADWGDTPLRFAAMIPLQVYNTLRV
EEEEECHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEHHEECHHHHHHHCCC
PEERKRLEQTDILIIGGGAIDAALEQEIRQMPNTVYSTYGMTETLSHVALRRLNGPEASP
CHHHHHHHHCCEEEEECCHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHCCCCCCCC
YYHPFPSVTLSLSPDNTLVIDAPLVCDERLVTNDVARLLPDGSFAIIGRKDNIINSGGIK
CCCCCCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEECCCCEECCCCCE
IQIEEVEERLRPYLDLPYAITAAPDPRLGEAVVLLIAPRSCETVSPPRVTPHMKAIMSDS
EEHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCC
AQLSCEVSTVALASLPKYLRPRHVIEASEIPQTGSGKIDRAACRELARNIITKTL
CCEEEEHHHHHHHHHHHHHCCCCEECHHCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626063; 9205837; 9278503 [H]