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The map label for this gene is nqrF [H]

Identifier: 150007762

GI number: 150007762

Start: 1293147

End: 1294433

Strand: Reverse

Name: nqrF [H]

Synonym: BDI_1120

Alternate gene names: 150007762

Gene position: 1294433-1293147 (Counterclockwise)

Preceding gene: 150007763

Following gene: 150007761

Centisome position: 26.9

GC content: 46.23

Gene sequence:

>1287_bases
ATGACTTTATTAATGTCGGGCGGACTTACGATTGGTGTCAGCGCAATAGTATTCTTATTGATCACGCTGATCTTGGTCGG
TTTGCTTTTGTTCGCCAAAGCAAAATTGGTGCCTTCAGGGAATGTAAGCTTGAAGGTTAACGGAGAGAAAGATATAGAGA
CCCCGATTGGTGGAACTTTGCTAGGTGCGCTACAGAGCGGTGGCATCTTCTTGTCTTCCGCTTGTGGTGGTGGTGGTAAA
TGCGGTCAGTGCCGTGCTCAAGTTATTGATGGCGGTGGTGAGATCCTACCTACTGAGAAAGGTTTCTTCTCTCGTAAACA
AGTGAAAGATCATTGGCGTTTGGCTTGTCAATGCAAGGTGAAAGAGGATATGGTCGTTCAAGTACCAGACGAGGTATTTG
GCGTTAAGGAATGGGAATGCGAGGTTATCAGTAACAAGAACGTCGCTACATTTATCAAGGAGTTTATCGTTGCGTTGCCT
AAAGGTGAGCACATGGACTTTATTCCGGGTTCTTACGCTCAGATCAAGATTCCTACATACTCAATGGATTATAACAAGGA
TATCGATAAGAGCTTGATCGGCCCCGAGTATCTGCCGGCTTGGGAGAAATTCGGTTTATTCGGTTTGAAGTGTAAGAACG
ACTCACCGTCTATCCGTGCCTATTCTATGGCTAACTATCCTGCTGAGGGTGACCGTATCATGTTGACCGTGCGTATCGCT
ACGCCGCCGTTCAAACCGAAACCACAAGTTGGTTTCATGGATGTGATGCCGGGTATCGCTTCTTCTTATATCTTTACGTT
GAAACCGGGTGATAAGGTGACTATGAGTGGTCCTTATGGTGATTTCCACCCAATTTTCGATTCTAAGAGAGAGATGATGT
GGATTGGTGGTGGTGCTGGTATGGCTCCGTTGCGTGCCCAAATCATGCACATGACGAAGACGTTGAAGACTACGGATCGT
AAGATGTCTTACTTCTATGGCGCACGTGCCTTGAACGAAGTGTTCTATTTGGAAGATTTCTTGGAAATAGAGAAAGAATT
CCCAAACTTTACGTTCCACTTGGCGCTTGACCGTCCTGATCCTGCCGCTGATGCTGCGGGTGTTAAATATACGGCTGGAT
TCGTTCATCAAGTAATCCATGATACTTACTTGAAGGATCACGAGGCTCCGGAAGATATCGAATACTACATGTGTGGTCCG
GGCCCGATGTCAAAGGCCGTGGAAAATATGCTGGATAACTTGGGTGTACCTCGTGAGATGTTGCACTTCGATGATTTCGG
TGGATAA

Upstream 100 bases:

>100_bases
TTGGGTATTACTTTCATCATCACGGGTTTGATGGGTATGGCATTCATGTGTTTCTCTGGTCTGAAGATTTAAGTATTAAC
GCATTAAACAAAATATAAAG

Downstream 100 bases:

>100_bases
AATAGGATTTCTATATCTGCAGATAGAAGGCGGGGGTTAAGATCCTCGCCTTTTTATTTTGGCCTTGAAACTCCTCTTTA
CGAAGATATACTTCAAATGC

Product: Na(+)-translocating NADH-quinone reductase subunit F

Products: @TETR01txt*tetrahydropteridine!; NO3-; NAD(P); Proton [C]

Alternate protein names: Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MTLLMSGGLTIGVSAIVFLLITLILVGLLLFAKAKLVPSGNVSLKVNGEKDIETPIGGTLLGALQSGGIFLSSACGGGGK
CGQCRAQVIDGGGEILPTEKGFFSRKQVKDHWRLACQCKVKEDMVVQVPDEVFGVKEWECEVISNKNVATFIKEFIVALP
KGEHMDFIPGSYAQIKIPTYSMDYNKDIDKSLIGPEYLPAWEKFGLFGLKCKNDSPSIRAYSMANYPAEGDRIMLTVRIA
TPPFKPKPQVGFMDVMPGIASSYIFTLKPGDKVTMSGPYGDFHPIFDSKREMMWIGGGAGMAPLRAQIMHMTKTLKTTDR
KMSYFYGARALNEVFYLEDFLEIEKEFPNFTFHLALDRPDPAADAAGVKYTAGFVHQVIHDTYLKDHEAPEDIEYYMCGP
GPMSKAVENMLDNLGVPREMLHFDDFGG

Sequences:

>Translated_428_residues
MTLLMSGGLTIGVSAIVFLLITLILVGLLLFAKAKLVPSGNVSLKVNGEKDIETPIGGTLLGALQSGGIFLSSACGGGGK
CGQCRAQVIDGGGEILPTEKGFFSRKQVKDHWRLACQCKVKEDMVVQVPDEVFGVKEWECEVISNKNVATFIKEFIVALP
KGEHMDFIPGSYAQIKIPTYSMDYNKDIDKSLIGPEYLPAWEKFGLFGLKCKNDSPSIRAYSMANYPAEGDRIMLTVRIA
TPPFKPKPQVGFMDVMPGIASSYIFTLKPGDKVTMSGPYGDFHPIFDSKREMMWIGGGAGMAPLRAQIMHMTKTLKTTDR
KMSYFYGARALNEVFYLEDFLEIEKEFPNFTFHLALDRPDPAADAAGVKYTAGFVHQVIHDTYLKDHEAPEDIEYYMCGP
GPMSKAVENMLDNLGVPREMLHFDDFGG
>Mature_427_residues
TLLMSGGLTIGVSAIVFLLITLILVGLLLFAKAKLVPSGNVSLKVNGEKDIETPIGGTLLGALQSGGIFLSSACGGGGKC
GQCRAQVIDGGGEILPTEKGFFSRKQVKDHWRLACQCKVKEDMVVQVPDEVFGVKEWECEVISNKNVATFIKEFIVALPK
GEHMDFIPGSYAQIKIPTYSMDYNKDIDKSLIGPEYLPAWEKFGLFGLKCKNDSPSIRAYSMANYPAEGDRIMLTVRIAT
PPFKPKPQVGFMDVMPGIASSYIFTLKPGDKVTMSGPYGDFHPIFDSKREMMWIGGGAGMAPLRAQIMHMTKTLKTTDRK
MSYFYGARALNEVFYLEDFLEIEKEFPNFTFHLALDRPDPAADAAGVKYTAGFVHQVIHDTYLKDHEAPEDIEYYMCGPG
PMSKAVENMLDNLGVPREMLHFDDFGG

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. The first step is catalyzed by nqrF, which accepts electrons from NADH and reduces

COG id: COG2871

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrF

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR010205
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.34 [C]

Molecular weight: Translated: 47168; Mature: 47037

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLMSGGLTIGVSAIVFLLITLILVGLLLFAKAKLVPSGNVSLKVNGEKDIETPIGGTL
CEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHH
LGALQSGGIFLSSACGGGGKCGQCRAQVIDGGGEILPTEKGFFSRKQVKDHWRLACQCKV
HHHHHCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHEEEEEEEE
KEDMVVQVPDEVFGVKEWECEVISNKNVATFIKEFIVALPKGEHMDFIPGSYAQIKIPTY
CCCEEEECCHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEE
SMDYNKDIDKSLIGPEYLPAWEKFGLFGLKCKNDSPSIRAYSMANYPAEGDRIMLTVRIA
ECCCCCCCHHHHCCCCCCCCHHHCCCEEEEECCCCCCEEEEEECCCCCCCCEEEEEEEEE
TPPFKPKPQVGFMDVMPGIASSYIFTLKPGDKVTMSGPYGDFHPIFDSKREMMWIGGGAG
CCCCCCCCCCCHHHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCEEEEEECCCC
MAPLRAQIMHMTKTLKTTDRKMSYFYGARALNEVFYLEDFLEIEKEFPNFTFHLALDRPD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
PAADAAGVKYTAGFVHQVIHDTYLKDHEAPEDIEYYMCGPGPMSKAVENMLDNLGVPREM
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCHHH
LHFDDFGG
HCCCCCCC
>Mature Secondary Structure 
TLLMSGGLTIGVSAIVFLLITLILVGLLLFAKAKLVPSGNVSLKVNGEKDIETPIGGTL
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHH
LGALQSGGIFLSSACGGGGKCGQCRAQVIDGGGEILPTEKGFFSRKQVKDHWRLACQCKV
HHHHHCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHEEEEEEEE
KEDMVVQVPDEVFGVKEWECEVISNKNVATFIKEFIVALPKGEHMDFIPGSYAQIKIPTY
CCCEEEECCHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEE
SMDYNKDIDKSLIGPEYLPAWEKFGLFGLKCKNDSPSIRAYSMANYPAEGDRIMLTVRIA
ECCCCCCCHHHHCCCCCCCCHHHCCCEEEEECCCCCCEEEEEECCCCCCCCEEEEEEEEE
TPPFKPKPQVGFMDVMPGIASSYIFTLKPGDKVTMSGPYGDFHPIFDSKREMMWIGGGAG
CCCCCCCCCCCHHHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCEEEEEECCCC
MAPLRAQIMHMTKTLKTTDRKMSYFYGARALNEVFYLEDFLEIEKEFPNFTFHLALDRPD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
PAADAAGVKYTAGFVHQVIHDTYLKDHEAPEDIEYYMCGPGPMSKAVENMLDNLGVPREM
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCHHH
LHFDDFGG
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dihydropteridine; 2 nitric oxide; O2; NAD(P)H [C]

Specific reaction: NAD(P)H + dihydropteridine = NAD(P)+ @TETR01.txt*tetrahydropteridine! 2 nitric oxide + 2 O2 + NAD(P)H = 2 NO3- + NAD(P) + Proton [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA