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The map label for this gene is cbiK [H]

Identifier: 150007715

GI number: 150007715

Start: 1244291

End: 1245211

Strand: Reverse

Name: cbiK [H]

Synonym: BDI_1070

Alternate gene names: 150007715

Gene position: 1245211-1244291 (Counterclockwise)

Preceding gene: 150007722

Following gene: 150007714

Centisome position: 25.88

GC content: 43.97

Gene sequence:

>921_bases
ATGATGAGGAAACACTTACTATCTTTCTTCCTGCTGGTCATCAGCCTAGTTTGTCGTGCGCACGATGGCGAGAATTTTGT
AGCATCCGATTTATTCGCCTCTTTACAACCGGGCGATAAGGCCGCTTTATTGATGGTACATTTTGGAACAACCCATGATG
ATACCCGTGCCTTGACAATCGACGCGATCAATCAAAAAGCGAAAGAAGCTTTCAAAGATGTTGAGTTGCGTGAGGCATGG
ACTTCTCGCATTGTAATGCGCCGCTTAAAAGCTCGTGGCGTAGAGAAACTGAACCCGATCGAGGCCTTGGAAAAATTAAA
GACTGATGGTTATACACATGTCTTGATCCAATCCACGAATATAATCGAAGGTATCGAGATGGAATCCTTACGTAAGGATG
TTGCCACCATGAAATCTTCTTTCAAGGAGATTCGTATCGGTAATCCTTTATTGTATACACCGGAGGATTATGAGGCGGTA
ATCGCCGCTATCATAAAAAATGGGGCGAAAGAAGGCGCTACTTTATTGGTAGGCCATGGCACTTATACACCGGCGACGGC
CCAATATGCTATGTTGGATTATATGTTGAAGGAGAAGGGATTCAAGGATTATTCCGTAGGAACCATCGAGGGCTATCCTA
CTTTTGATACGATGGTAGCCCAAGTAAAGGCCAATGGAACGAAGAAAGTCTTGTTGATGCCATTTATGTTTGTTGCCGGA
GACCATGCGAAAAACGATATCGCCGGTGATTGGAAAGAGGAATTGGAAAAGAAAGGCTACGAGGTAAGCGTATTTATGGA
AGGTCTGGGACAGAATCCGGATATCCAGAAGATCTTCATCGAACATGCCCGTTTCATGGCTAAACATAAGATGATCGATA
TCGTTGATAAGAAGAAAGCTTATGCGGTAGAGAAGGACTAA

Upstream 100 bases:

>100_bases
AAGAGGATAGAAATATCACCTATATCTAAAAGATGTTGGAGACGGAATAATACGTTGTCTCCGTGGGATTGATATGTAAT
CTAATTATTAATATATTCAT

Downstream 100 bases:

>100_bases
AAAAGTTCACTACACAATGTTTTTTATTCCTTTCGGAAGAAAAAGTAATTCCTTCCGAAAGAAACTAAATTTTCATTCGA
AGGAAAATGGAATTTCTTTC

Product: anaerobic cobalt chelatase CbiK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MMRKHLLSFFLLVISLVCRAHDGENFVASDLFASLQPGDKAALLMVHFGTTHDDTRALTIDAINQKAKEAFKDVELREAW
TSRIVMRRLKARGVEKLNPIEALEKLKTDGYTHVLIQSTNIIEGIEMESLRKDVATMKSSFKEIRIGNPLLYTPEDYEAV
IAAIIKNGAKEGATLLVGHGTYTPATAQYAMLDYMLKEKGFKDYSVGTIEGYPTFDTMVAQVKANGTKKVLLMPFMFVAG
DHAKNDIAGDWKEELEKKGYEVSVFMEGLGQNPDIQKIFIEHARFMAKHKMIDIVDKKKAYAVEKD

Sequences:

>Translated_306_residues
MMRKHLLSFFLLVISLVCRAHDGENFVASDLFASLQPGDKAALLMVHFGTTHDDTRALTIDAINQKAKEAFKDVELREAW
TSRIVMRRLKARGVEKLNPIEALEKLKTDGYTHVLIQSTNIIEGIEMESLRKDVATMKSSFKEIRIGNPLLYTPEDYEAV
IAAIIKNGAKEGATLLVGHGTYTPATAQYAMLDYMLKEKGFKDYSVGTIEGYPTFDTMVAQVKANGTKKVLLMPFMFVAG
DHAKNDIAGDWKEELEKKGYEVSVFMEGLGQNPDIQKIFIEHARFMAKHKMIDIVDKKKAYAVEKD
>Mature_306_residues
MMRKHLLSFFLLVISLVCRAHDGENFVASDLFASLQPGDKAALLMVHFGTTHDDTRALTIDAINQKAKEAFKDVELREAW
TSRIVMRRLKARGVEKLNPIEALEKLKTDGYTHVLIQSTNIIEGIEMESLRKDVATMKSSFKEIRIGNPLLYTPEDYEAV
IAAIIKNGAKEGATLLVGHGTYTPATAQYAMLDYMLKEKGFKDYSVGTIEGYPTFDTMVAQVKANGTKKVLLMPFMFVAG
DHAKNDIAGDWKEELEKKGYEVSVFMEGLGQNPDIQKIFIEHARFMAKHKMIDIVDKKKAYAVEKD

Specific function: Chelates cobalt to the cobalamin precursor as part of the anaerobic pathway to cobalamin biosynthesis [H]

COG id: COG4822

COG function: function code H; Cobalamin biosynthesis protein CbiK, Co2+ chelatase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CbiK family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010388 [H]

Pfam domain/function: PF06180 CbiK [H]

EC number: =4.99.1.3 [H]

Molecular weight: Translated: 34386; Mature: 34386

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRKHLLSFFLLVISLVCRAHDGENFVASDLFASLQPGDKAALLMVHFGTTHDDTRALTI
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEEH
DAINQKAKEAFKDVELREAWTSRIVMRRLKARGVEKLNPIEALEKLKTDGYTHVLIQSTN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEECCC
IIEGIEMESLRKDVATMKSSFKEIRIGNPLLYTPEDYEAVIAAIIKNGAKEGATLLVGHG
CCCCCCHHHHHHHHHHHHHHHHHEECCCCEEECCCHHHHHHHHHHHCCCCCCCEEEEECC
TYTPATAQYAMLDYMLKEKGFKDYSVGTIEGYPTFDTMVAQVKANGTKKVLLMPFMFVAG
CCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHCC
DHAKNDIAGDWKEELEKKGYEVSVFMEGLGQNPDIQKIFIEHARFMAKHKMIDIVDKKKA
CCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
YAVEKD
CCCCCC
>Mature Secondary Structure
MMRKHLLSFFLLVISLVCRAHDGENFVASDLFASLQPGDKAALLMVHFGTTHDDTRALTI
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEEH
DAINQKAKEAFKDVELREAWTSRIVMRRLKARGVEKLNPIEALEKLKTDGYTHVLIQSTN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEECCC
IIEGIEMESLRKDVATMKSSFKEIRIGNPLLYTPEDYEAVIAAIIKNGAKEGATLLVGHG
CCCCCCHHHHHHHHHHHHHHHHHEECCCCEEECCCHHHHHHHHHHHCCCCCCCEEEEECC
TYTPATAQYAMLDYMLKEKGFKDYSVGTIEGYPTFDTMVAQVKANGTKKVLLMPFMFVAG
CCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHCC
DHAKNDIAGDWKEELEKKGYEVSVFMEGLGQNPDIQKIFIEHARFMAKHKMIDIVDKKKA
CCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
YAVEKD
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609; 9150215; 10451360 [H]