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The map label for this gene is gacS [H]

Identifier: 150007677

GI number: 150007677

Start: 1197674

End: 1198768

Strand: Direct

Name: gacS [H]

Synonym: BDI_1032

Alternate gene names: 150007677

Gene position: 1197674-1198768 (Clockwise)

Preceding gene: 150007676

Following gene: 150007678

Centisome position: 24.89

GC content: 42.83

Gene sequence:

>1095_bases
ATGAAAACGACATTAGGCATAGAGTTCGAGCTTTTTTTTCCTCAGATAAGTCATCGTGCCAAGATCGGCTGCGCAAAGTA
TGATTTGTCCACTGGGGAAGGAGTGGCGATGGAACAATGGTATCTGAATATGGGTGAAGTTCCGGGAACTCCATTGAGCC
AGATAGTAGGCATATATTCCAATGTCAAGCCAGAAGACCGTGAATGCTTGAAAACTTCCTTGAGCCGACTTGTCCACGGA
GAAACTGATCATGATCAACGGGAAGCCCGGGTCAAGGATGGCGAGGGATGGAAATGGATTCGTCTGGATATAATGGAGGC
GGGTCTGGAGAAATCAAGCCCCATCCTATTACTGATGAATTATGATATATCTGAACTGAAAGCGATGGAAGAACGAATGC
TGAAGGCCGAGAAATCAGAGCGTTTGAAATCCTCCTTTTTGGCAAACATCAGCCATGAGATCCGCATGCCATTAAACGCT
ATTGTCGGATTCTCTTCTCTTTTAGGGGATGAAGAGGATGGTGAGCTAAGACAAGAGTATATTAATCTTATTCAGACCAA
TAATGAGTTATTATTGGGTATCGTTAATGATGTATTGGATCTGGCTAAGCTCGAGTCTGGTACCATGACCTTTGATTTCA
TGGAATTTGATCTAAGACAACTTATTGTAGAAACGGTAGCTTCTTTCCAGATCAAAGTACCGAGAGGAGTAGCTTTGACC
TATCCGGCATCTTTGGATTCCTTTTTATTACGCTCCGACCGTATCCGTTTGAAACAAGTTCTTGGCAATTTTATAACAAA
CGCAATTAAGTACACCTCTATAGGTAGCATCATCTTGTCATATCAAGCCATGGAGAACGAGGTTCTGTTATCCGTGACCG
ATACGGGGGAAGGAATGTCGGAGGAAATAAAACTTCATGTGTTCGATCGTTTTTATAAAGGACGAAACCAAAAGCAAGGC
ATAGGGCTTGGACTTTCTATCTGCCAAAATATAATTGAAAGGCTGGGAGGGCGTATCGGTGTTTCTTCTGAGCAGGGAAA
AGGCTCTTGTTTTTGGTGCACTTTACCAATCTTTCCCCCAAAAGATACCTATTAA

Upstream 100 bases:

>100_bases
AAATAATCGATATAAACGACTAATTAAAACGAACGATTAATTCATTCGTTTTTTATGGCTAAACCCTCTTTTTGCATAAA
TATAATGAGAACGGATATAT

Downstream 100 bases:

>100_bases
ATAAAGAAAATACCGTATATTTGTTTTTGAAAGAGAAACGAAAAAACAATTTCTACGTATGGAGCGGTATTTAATTATAA
AAACAAGGGATGAGTTGTTG

Product: putative two-component system sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKTTLGIEFELFFPQISHRAKIGCAKYDLSTGEGVAMEQWYLNMGEVPGTPLSQIVGIYSNVKPEDRECLKTSLSRLVHG
ETDHDQREARVKDGEGWKWIRLDIMEAGLEKSSPILLLMNYDISELKAMEERMLKAEKSERLKSSFLANISHEIRMPLNA
IVGFSSLLGDEEDGELRQEYINLIQTNNELLLGIVNDVLDLAKLESGTMTFDFMEFDLRQLIVETVASFQIKVPRGVALT
YPASLDSFLLRSDRIRLKQVLGNFITNAIKYTSIGSIILSYQAMENEVLLSVTDTGEGMSEEIKLHVFDRFYKGRNQKQG
IGLGLSICQNIIERLGGRIGVSSEQGKGSCFWCTLPIFPPKDTY

Sequences:

>Translated_364_residues
MKTTLGIEFELFFPQISHRAKIGCAKYDLSTGEGVAMEQWYLNMGEVPGTPLSQIVGIYSNVKPEDRECLKTSLSRLVHG
ETDHDQREARVKDGEGWKWIRLDIMEAGLEKSSPILLLMNYDISELKAMEERMLKAEKSERLKSSFLANISHEIRMPLNA
IVGFSSLLGDEEDGELRQEYINLIQTNNELLLGIVNDVLDLAKLESGTMTFDFMEFDLRQLIVETVASFQIKVPRGVALT
YPASLDSFLLRSDRIRLKQVLGNFITNAIKYTSIGSIILSYQAMENEVLLSVTDTGEGMSEEIKLHVFDRFYKGRNQKQG
IGLGLSICQNIIERLGGRIGVSSEQGKGSCFWCTLPIFPPKDTY
>Mature_364_residues
MKTTLGIEFELFFPQISHRAKIGCAKYDLSTGEGVAMEQWYLNMGEVPGTPLSQIVGIYSNVKPEDRECLKTSLSRLVHG
ETDHDQREARVKDGEGWKWIRLDIMEAGLEKSSPILLLMNYDISELKAMEERMLKAEKSERLKSSFLANISHEIRMPLNA
IVGFSSLLGDEEDGELRQEYINLIQTNNELLLGIVNDVLDLAKLESGTMTFDFMEFDLRQLIVETVASFQIKVPRGVALT
YPASLDSFLLRSDRIRLKQVLGNFITNAIKYTSIGSIILSYQAMENEVLLSVTDTGEGMSEEIKLHVFDRFYKGRNQKQG
IGLGLSICQNIIERLGGRIGVSSEQGKGSCFWCTLPIFPPKDTY

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=229, Percent_Identity=34.061135371179, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1788713, Length=251, Percent_Identity=34.2629482071713, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI145693157, Length=252, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI48994928, Length=248, Percent_Identity=31.8548387096774, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI87081816, Length=232, Percent_Identity=31.8965517241379, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1786912, Length=244, Percent_Identity=28.2786885245902, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788549, Length=249, Percent_Identity=29.3172690763052, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788393, Length=229, Percent_Identity=30.5676855895196, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082128, Length=247, Percent_Identity=27.9352226720648, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1786600, Length=246, Percent_Identity=24.390243902439, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790436, Length=235, Percent_Identity=30.6382978723404, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786783, Length=223, Percent_Identity=27.3542600896861, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789808, Length=284, Percent_Identity=24.6478873239437, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790861, Length=227, Percent_Identity=25.5506607929515, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41010; Mature: 41010

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTLGIEFELFFPQISHRAKIGCAKYDLSTGEGVAMEQWYLNMGEVPGTPLSQIVGIYS
CCCEEEEEEEEECCCCCCHHHCCEEEEECCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHH
NVKPEDRECLKTSLSRLVHGETDHDQREARVKDGEGWKWIRLDIMEAGLEKSSPILLLMN
CCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEEEHHHCCCCCCCCEEEEEC
YDISELKAMEERMLKAEKSERLKSSFLANISHEIRMPLNAIVGFSSLLGDEEDGELRQEY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHH
INLIQTNNELLLGIVNDVLDLAKLESGTMTFDFMEFDLRQLIVETVASFQIKVPRGVALT
HHHHHCCCCEEHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHEEEECCCCEEEE
YPASLDSFLLRSDRIRLKQVLGNFITNAIKYTSIGSIILSYQAMENEVLLSVTDTGEGMS
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
EEIKLHVFDRFYKGRNQKQGIGLGLSICQNIIERLGGRIGVSSEQGKGSCFWCTLPIFPP
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCC
KDTY
CCCC
>Mature Secondary Structure
MKTTLGIEFELFFPQISHRAKIGCAKYDLSTGEGVAMEQWYLNMGEVPGTPLSQIVGIYS
CCCEEEEEEEEECCCCCCHHHCCEEEEECCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHH
NVKPEDRECLKTSLSRLVHGETDHDQREARVKDGEGWKWIRLDIMEAGLEKSSPILLLMN
CCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEEEHHHCCCCCCCCEEEEEC
YDISELKAMEERMLKAEKSERLKSSFLANISHEIRMPLNAIVGFSSLLGDEEDGELRQEY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHH
INLIQTNNELLLGIVNDVLDLAKLESGTMTFDFMEFDLRQLIVETVASFQIKVPRGVALT
HHHHHCCCCEEHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHEEEECCCCEEEE
YPASLDSFLLRSDRIRLKQVLGNFITNAIKYTSIGSIILSYQAMENEVLLSVTDTGEGMS
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
EEIKLHVFDRFYKGRNQKQGIGLGLSICQNIIERLGGRIGVSSEQGKGSCFWCTLPIFPP
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCC
KDTY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]