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The map label for this gene is oprF [H]

Identifier: 150007641

GI number: 150007641

Start: 1158652

End: 1159989

Strand: Direct

Name: oprF [H]

Synonym: BDI_0996

Alternate gene names: 150007641

Gene position: 1158652-1159989 (Clockwise)

Preceding gene: 150007640

Following gene: 150007642

Centisome position: 24.08

GC content: 47.83

Gene sequence:

>1338_bases
ATGAAAAAGCTAGTATTTATTTTATCTGCCATGTTGCTCCTAATCGGGACAATGGACGTGCAAGCCCAATGGGGAAAGAA
ACTCCTCAAGAAAGCGGGTGAGAGTGCCAAACGGGCAACGGAGAGGAACGTGGAACGGAAAGTGGAACAGACCGTAGACA
AGGCTTTCGAGGGAGCGGAGGATGTGGTGACGGGGAAAGGGAATAATAATAATGAAAAGGAGGAGCCACCAACAAGTACA
AATTCACAAGGGAATTTTGAAACTATGGATGATGGGGAAGATGTGGCACAGCAGGAACAGCAACAACCACAGCAATCACT
CGAAATGACGTATGCCAAGAGTGACTTCGTGCCGGGGGATGAGATTATTTTTGAGGATGATGTGACCCGGGAACAGATAG
GAGAATTCCCCAGCCAGTGGGATTTATTAAAAGGTACTGTTGAGATCGCTCAAATCAATGGCGATAAGGTGATACTTTGC
GTGAGTCAGGATGATCCGGTCATTGCGCCGCTATTCGATAATATGAAAAGTTATTTGCCGGAAAAGTTCACGCTGGAATT
TGACTTTTGGGTAGGGCCTTTTACAAAAAAGGGAGATGATGAGCCGGAAAATTGCTATATCGTACGTTTTTACAAACCCG
AATCGGGAAGTCGTGTTCAAACTATCAATCTTGACGACTGGTATTATAGTGCTTCAGACCATCGTACACGCCTAAGATGG
GACTTTATTCCTTCCTCTGGGGAGAACAGCCGTAGCGGAAGCGATGACTCTTTTCGGACTATCCCGAACTCATGGAACCA
CTTCTCACTGTCCTTCAATCAACGGGCAATGAAAGTTTACATAAATGGTATCCGCTATGCCAATATTCCGAATACGGTAG
CACCCGGACATTTTGACATACAGTTTTATAGAGGCGGTGGATATTTTACGAATAACACTTCCATCCGCAACATACGTGTC
GCCAAAGGCGCAGTGCCATTGTACGACCGCATGATGAGCGACGGTAAGTTCATCACGTATGGCATCACCTTCGATGTCGG
CAAGTCCACCATCAAACCAGAGAGTATGGGTGAGATCAATCGTATCGTCAAATTAATGACCGATAACCCCGACCTGAGAT
TCTCCGTAGAAGGCCATACCGATAGCACCGGCAACGAGGCTTCCAACCAGACGTTAAGCGAGGCCCGCAGCAACGCCATC
GTAGGTAAACTGGTTGAACTGGGCATCAGTGCCGATCGCCTCTCCGCTTCCGGCAAAGGACAATCATCTCCCATCGCCGA
TAACGGAACGGACGAGGGTCGTGCCAAGAACCGGCGGGTGGAGTTTGTCAAGATGTAA

Upstream 100 bases:

>100_bases
TCGCTTTTTTCGGAGATCCTTGATAAATCTGGACCGATCCATCAGAAGGTGTTTCGGTAAATAAATAGGAATGAATTATT
AATCTAATGAATTAAAGGAA

Downstream 100 bases:

>100_bases
CGAATAAGTGAGCGATATGAAAAAGAAATTCTACATACTGATGTGCTTGGCGAGCCTCGTCTTGATGGTGACGGCTTGCG
GCGGCAAGGAGAAGATCAAG

Product: outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MKKLVFILSAMLLLIGTMDVQAQWGKKLLKKAGESAKRATERNVERKVEQTVDKAFEGAEDVVTGKGNNNNEKEEPPTST
NSQGNFETMDDGEDVAQQEQQQPQQSLEMTYAKSDFVPGDEIIFEDDVTREQIGEFPSQWDLLKGTVEIAQINGDKVILC
VSQDDPVIAPLFDNMKSYLPEKFTLEFDFWVGPFTKKGDDEPENCYIVRFYKPESGSRVQTINLDDWYYSASDHRTRLRW
DFIPSSGENSRSGSDDSFRTIPNSWNHFSLSFNQRAMKVYINGIRYANIPNTVAPGHFDIQFYRGGGYFTNNTSIRNIRV
AKGAVPLYDRMMSDGKFITYGITFDVGKSTIKPESMGEINRIVKLMTDNPDLRFSVEGHTDSTGNEASNQTLSEARSNAI
VGKLVELGISADRLSASGKGQSSPIADNGTDEGRAKNRRVEFVKM

Sequences:

>Translated_445_residues
MKKLVFILSAMLLLIGTMDVQAQWGKKLLKKAGESAKRATERNVERKVEQTVDKAFEGAEDVVTGKGNNNNEKEEPPTST
NSQGNFETMDDGEDVAQQEQQQPQQSLEMTYAKSDFVPGDEIIFEDDVTREQIGEFPSQWDLLKGTVEIAQINGDKVILC
VSQDDPVIAPLFDNMKSYLPEKFTLEFDFWVGPFTKKGDDEPENCYIVRFYKPESGSRVQTINLDDWYYSASDHRTRLRW
DFIPSSGENSRSGSDDSFRTIPNSWNHFSLSFNQRAMKVYINGIRYANIPNTVAPGHFDIQFYRGGGYFTNNTSIRNIRV
AKGAVPLYDRMMSDGKFITYGITFDVGKSTIKPESMGEINRIVKLMTDNPDLRFSVEGHTDSTGNEASNQTLSEARSNAI
VGKLVELGISADRLSASGKGQSSPIADNGTDEGRAKNRRVEFVKM
>Mature_445_residues
MKKLVFILSAMLLLIGTMDVQAQWGKKLLKKAGESAKRATERNVERKVEQTVDKAFEGAEDVVTGKGNNNNEKEEPPTST
NSQGNFETMDDGEDVAQQEQQQPQQSLEMTYAKSDFVPGDEIIFEDDVTREQIGEFPSQWDLLKGTVEIAQINGDKVILC
VSQDDPVIAPLFDNMKSYLPEKFTLEFDFWVGPFTKKGDDEPENCYIVRFYKPESGSRVQTINLDDWYYSASDHRTRLRW
DFIPSSGENSRSGSDDSFRTIPNSWNHFSLSFNQRAMKVYINGIRYANIPNTVAPGHFDIQFYRGGGYFTNNTSIRNIRV
AKGAVPLYDRMMSDGKFITYGITFDVGKSTIKPESMGEINRIVKLMTDNPDLRFSVEGHTDSTGNEASNQTLSEARSNAI
VGKLVELGISADRLSASGKGQSSPIADNGTDEGRAKNRRVEFVKM

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=101, Percent_Identity=36.6336633663366, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787191, Length=112, Percent_Identity=34.8214285714286, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 49934; Mature: 49934

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVFILSAMLLLIGTMDVQAQWGKKLLKKAGESAKRATERNVERKVEQTVDKAFEGAE
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
DVVTGKGNNNNEKEEPPTSTNSQGNFETMDDGEDVAQQEQQQPQQSLEMTYAKSDFVPGD
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCCC
EIIFEDDVTREQIGEFPSQWDLLKGTVEIAQINGDKVILCVSQDDPVIAPLFDNMKSYLP
CEEECCCCCHHHHHCCCCHHHHHHCEEEEEEECCCEEEEEECCCCCEEHHHHHHHHHHCC
EKFTLEFDFWVGPFTKKGDDEPENCYIVRFYKPESGSRVQTINLDDWYYSASDHRTRLRW
CEEEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCEECCCCCCEEEEE
DFIPSSGENSRSGSDDSFRTIPNSWNHFSLSFNQRAMKVYINGIRYANIPNTVAPGHFDI
EECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECEEEECCCCCCCCCEEEE
QFYRGGGYFTNNTSIRNIRVAKGAVPLYDRMMSDGKFITYGITFDVGKSTIKPESMGEIN
EEEECCCEEECCCCEEEEEEECCCCHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHH
RIVKLMTDNPDLRFSVEGHTDSTGNEASNQTLSEARSNAIVGKLVELGISADRLSASGKG
HHHHHEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
QSSPIADNGTDEGRAKNRRVEFVKM
CCCCCCCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MKKLVFILSAMLLLIGTMDVQAQWGKKLLKKAGESAKRATERNVERKVEQTVDKAFEGAE
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
DVVTGKGNNNNEKEEPPTSTNSQGNFETMDDGEDVAQQEQQQPQQSLEMTYAKSDFVPGD
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCCC
EIIFEDDVTREQIGEFPSQWDLLKGTVEIAQINGDKVILCVSQDDPVIAPLFDNMKSYLP
CEEECCCCCHHHHHCCCCHHHHHHCEEEEEEECCCEEEEEECCCCCEEHHHHHHHHHHCC
EKFTLEFDFWVGPFTKKGDDEPENCYIVRFYKPESGSRVQTINLDDWYYSASDHRTRLRW
CEEEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCEECCCCCCEEEEE
DFIPSSGENSRSGSDDSFRTIPNSWNHFSLSFNQRAMKVYINGIRYANIPNTVAPGHFDI
EECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECEEEECCCCCCCCCEEEE
QFYRGGGYFTNNTSIRNIRVAKGAVPLYDRMMSDGKFITYGITFDVGKSTIKPESMGEIN
EEEECCCEEECCCCEEEEEEECCCCHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHH
RIVKLMTDNPDLRFSVEGHTDSTGNEASNQTLSEARSNAIVGKLVELGISADRLSASGKG
HHHHHEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
QSSPIADNGTDEGRAKNRRVEFVKM
CCCCCCCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]