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The map label for this gene is eco47IIM [H]

Identifier: 150007509

GI number: 150007509

Start: 1002546

End: 1003829

Strand: Direct

Name: eco47IIM [H]

Synonym: BDI_0861

Alternate gene names: 150007509

Gene position: 1002546-1003829 (Clockwise)

Preceding gene: 150007508

Following gene: 150007510

Centisome position: 20.84

GC content: 49.22

Gene sequence:

>1284_bases
ATGACACATGGATCATTATTTTCTGGCGTGGGCGGTTTTGACCTTGCCGCCGAATGGATGGGATGGGAGAACCTGTTCCA
TTGCGAGATTAACGAGTGGTGCCAAAAGGTACTGAGGTTTCATTTCCCAAAAAGCATTCAATATGACGATATTACAAGAA
CTGATTTCACTCCGTGGAGAGGGAAGGTTGACGTACTCACAGGAGGGTTCCCTTGTCAGCCATTTTCAACGGCAGGAAAG
CGAAGGGGAGCGGAAGATGACCGTTACCTCTGGCCGGAAATGCTTCGGGCAATACGGGAGATACGACCCGCTTGGGTCAT
TGGTGAGAACGTTGCTGGAATCACCAGCATGGTACAACCCGGCAGTGAGGTTACGGTGGAAAGTCAAGCCTCTTTGTTTG
AAAAGGCTGACAAGGAAACAATACTCGAACAAGAGTACGTTATCGAAACCGTCTGCCGAGATCTTGAACGTGAGGGATAT
TCCGTCCAGCCGATTCTTATTCCAGCTTGCGGTGTCGGAGCGCCGCACAAGAGGGACAGGGTATGGTTTATTGCCCACTC
CGAGGGCGATGGAGATCGTGGAGCATCCCATGAAGGCGGCGGAACGACTCAAGGATCGTACGGGAAAGAAATTGAACAAT
CTTTCATCGGGAGCGGCATTTGGACTACTTCCCACCCCAACTGCTCAAGAGGGCTTCAATTCGGGGAAAGGAGAGATATT
CGTGACAAGGAACAATACGATCAGGATAAGGAACCAGAACGGCACGAGCAGCCGTCTAGGTTTGGAGGGAGCGGTGAAGC
ATATGTTATATCCGACACCGACAGCCCAAGATTTCAAGCGAAGGGGTCCGAACAGCAAACAACAGGGATTACCGGAGGCG
GCCTACGAAAAGATGTTACCGACACCTACGGCGAGAAGCTACAAACATGGCTCAAAAATAACGGACGGGAGATCGAGGAG
GAAAATATCGCAAGGCTGGACAATGGAGTTGAACGATCTTGCTGTATCAGGGCTTTTGCCGAATCCGACAAGAAGAGACG
ATGCCTTATGCAATATACCAGTAATGATTGGCCAGCATTGTCAGCAGAACTATGGAAAGACTTCCCAACTCAACCCCCTG
TTTGTCGAAGAAATGATGGGTTACCCTTTGATGTGGACTACCTTGCCATTCCTTTCACAAAATGGAGACAAGAATCCATA
AAAGCCTACGGAAACGCCATCGTCCCACAAGTAGCATTTGAGATATTCAAGGCGATAGAAACATCAACCTTTCATCATAG
TTGA

Upstream 100 bases:

>100_bases
TGTAAGCGTTGCAGATCGGACACTGCGAATACAGCAAGGCTTGCATGGCCCATCTGAGGCCGGACCATGAAAGCGTAGTT
TTTGCTAAAACGAGAGAGAC

Downstream 100 bases:

>100_bases
AAACTGCATTCATCTATGATGAGAGCAAAGAAAGAATATAAAATTATATGAGAACACCAATCACATATTATGGAGGCAAG
CAAAACTTGTCCGAACGCAT

Product: site-specific DNA-methyltransferase

Products: NA

Alternate protein names: M.Eco47II; Cytosine-specific methyltransferase Eco47II [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTHGSLFSGVGGFDLAAEWMGWENLFHCEINEWCQKVLRFHFPKSIQYDDITRTDFTPWRGKVDVLTGGFPCQPFSTAGK
RRGAEDDRYLWPEMLRAIREIRPAWVIGENVAGITSMVQPGSEVTVESQASLFEKADKETILEQEYVIETVCRDLEREGY
SVQPILIPACGVGAPHKRDRVWFIAHSEGDGDRGASHEGGGTTQGSYGKEIEQSFIGSGIWTTSHPNCSRGLQFGERRDI
RDKEQYDQDKEPERHEQPSRFGGSGEAYVISDTDSPRFQAKGSEQQTTGITGGGLRKDVTDTYGEKLQTWLKNNGREIEE
ENIARLDNGVERSCCIRAFAESDKKRRCLMQYTSNDWPALSAELWKDFPTQPPVCRRNDGLPFDVDYLAIPFTKWRQESI
KAYGNAIVPQVAFEIFKAIETSTFHHS

Sequences:

>Translated_427_residues
MTHGSLFSGVGGFDLAAEWMGWENLFHCEINEWCQKVLRFHFPKSIQYDDITRTDFTPWRGKVDVLTGGFPCQPFSTAGK
RRGAEDDRYLWPEMLRAIREIRPAWVIGENVAGITSMVQPGSEVTVESQASLFEKADKETILEQEYVIETVCRDLEREGY
SVQPILIPACGVGAPHKRDRVWFIAHSEGDGDRGASHEGGGTTQGSYGKEIEQSFIGSGIWTTSHPNCSRGLQFGERRDI
RDKEQYDQDKEPERHEQPSRFGGSGEAYVISDTDSPRFQAKGSEQQTTGITGGGLRKDVTDTYGEKLQTWLKNNGREIEE
ENIARLDNGVERSCCIRAFAESDKKRRCLMQYTSNDWPALSAELWKDFPTQPPVCRRNDGLPFDVDYLAIPFTKWRQESI
KAYGNAIVPQVAFEIFKAIETSTFHHS
>Mature_426_residues
THGSLFSGVGGFDLAAEWMGWENLFHCEINEWCQKVLRFHFPKSIQYDDITRTDFTPWRGKVDVLTGGFPCQPFSTAGKR
RGAEDDRYLWPEMLRAIREIRPAWVIGENVAGITSMVQPGSEVTVESQASLFEKADKETILEQEYVIETVCRDLEREGYS
VQPILIPACGVGAPHKRDRVWFIAHSEGDGDRGASHEGGGTTQGSYGKEIEQSFIGSGIWTTSHPNCSRGLQFGERRDIR
DKEQYDQDKEPERHEQPSRFGGSGEAYVISDTDSPRFQAKGSEQQTTGITGGGLRKDVTDTYGEKLQTWLKNNGREIEEE
NIARLDNGVERSCCIRAFAESDKKRRCLMQYTSNDWPALSAELWKDFPTQPPVCRRNDGLPFDVDYLAIPFTKWRQESIK
AYGNAIVPQVAFEIFKAIETSTFHHS

Specific function: This methylase recognizes the double-stranded sequence GGNCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the Eco47II endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525
- InterPro:   IPR009061
- InterPro:   IPR000551 [H]

Pfam domain/function: PF00145 DNA_methylase; PF00376 MerR [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 48314; Mature: 48183

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHGSLFSGVGGFDLAAEWMGWENLFHCEINEWCQKVLRFHFPKSIQYDDITRTDFTPWR
CCCCCHHCCCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GKVDVLTGGFPCQPFSTAGKRRGAEDDRYLWPEMLRAIREIRPAWVIGENVAGITSMVQP
CEEEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCC
GSEVTVESQASLFEKADKETILEQEYVIETVCRDLEREGYSVQPILIPACGVGAPHKRDR
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCE
VWFIAHSEGDGDRGASHEGGGTTQGSYGKEIEQSFIGSGIWTTSHPNCSRGLQFGERRDI
EEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCC
RDKEQYDQDKEPERHEQPSRFGGSGEAYVISDTDSPRFQAKGSEQQTTGITGGGLRKDVT
CCHHHCCCCCCCHHHCCCHHCCCCCCEEEEECCCCCCEECCCCCCCCCCCCCCCCCHHHH
DTYGEKLQTWLKNNGREIEEENIARLDNGVERSCCIRAFAESDKKRRCLMQYTSNDWPAL
HHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCH
SAELWKDFPTQPPVCRRNDGLPFDVDYLAIPFTKWRQESIKAYGNAIVPQVAFEIFKAIE
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
TSTFHHS
HHCCCCC
>Mature Secondary Structure 
THGSLFSGVGGFDLAAEWMGWENLFHCEINEWCQKVLRFHFPKSIQYDDITRTDFTPWR
CCCCHHCCCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GKVDVLTGGFPCQPFSTAGKRRGAEDDRYLWPEMLRAIREIRPAWVIGENVAGITSMVQP
CEEEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCC
GSEVTVESQASLFEKADKETILEQEYVIETVCRDLEREGYSVQPILIPACGVGAPHKRDR
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCE
VWFIAHSEGDGDRGASHEGGGTTQGSYGKEIEQSFIGSGIWTTSHPNCSRGLQFGERRDI
EEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCC
RDKEQYDQDKEPERHEQPSRFGGSGEAYVISDTDSPRFQAKGSEQQTTGITGGGLRKDVT
CCHHHCCCCCCCHHHCCCHHCCCCCCEEEEECCCCCCEECCCCCCCCCCCCCCCCCHHHH
DTYGEKLQTWLKNNGREIEEENIARLDNGVERSCCIRAFAESDKKRRCLMQYTSNDWPAL
HHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCH
SAELWKDFPTQPPVCRRNDGLPFDVDYLAIPFTKWRQESIKAYGNAIVPQVAFEIFKAIE
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
TSTFHHS
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7607524 [H]