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The map label for this gene is gacS [H]

Identifier: 150007414

GI number: 150007414

Start: 893576

End: 895957

Strand: Reverse

Name: gacS [H]

Synonym: BDI_0765

Alternate gene names: 150007414

Gene position: 895957-893576 (Counterclockwise)

Preceding gene: 150007415

Following gene: 150007413

Centisome position: 18.62

GC content: 44.08

Gene sequence:

>2382_bases
ATGGAAGGGCGAGAGACGGATCTAGAATACAACTTATTGATGGGGCTTACGGGAGCTAGCGTCAGCAAACATTTATTGGA
TGAGCATTTCACACTTTTATGGGCAAATGACTTCTATTACGATATGATAGGTTACCCCAAAGAAGAATATGAAGCTCTTT
TCCACAATAAACCCGATTTGTATTTTAAAGGTCATGATGAAGCTTGGAATATATTATCCAAGAATGTTTACGAGACGATA
GCTAATAAAGCCAGCGGTTACGAAACCGTTATCCAGATGCCAACGAGAAAAGGGAAAAGATGGACTAAAATCACCTCAAC
GTTTACCGACCAACTACAAGACGGCATCCCTATCTCCTATACGGTAATGACCGATGTAGAGGACGTAATGCGAAGGCGGA
TCGAGCAAACGATCACTTATGATAATATCCCCGGCTTCATATCCAAGCATAAGGTACATAAAGACGGCAGTTTTACATTG
CTGGAGGCAAATGATAAATATATGGACTTCTCTGGTATCGATAAAAATTCTTTTTTATCTTTCAGTCCTTTCTCCCGTTT
AGATGAAACAAGCCGTAATACGATGAACGCACATATCCCCTGTATGCTAAAAGGGGAACCTGTACATTTCGTTATCCAGT
CGAAAGACAAAAACGGCAACGGCGCTTGGCTACAGTTGAACGGGGAATGTATCGGCTGGGAAGGTGACGAGCCTATCTAT
CTTATCGTTTACATCAATATCACCGACATTACCGAACAGAGGGAGTTGCAAAAGAAACTGGAGAAACAGTCAAAGCAACT
TAAAGAAGCACTAAAGTCGGCGGAACAAGCCAACCAAGCCAAATCTGATTTTCTCGCCCGCATGAGCCACGATATCCGTA
CACCCATGAACGCCATCATGGGAATGGCGACTATAGCCAAAGCCCATGTAGATGAACGTGAAAGAATATTAGATTGTATG
GAAAAAATCAATGGAGCCTCTAAATTATTATTGAGTCTGATTAATGAGGTTCTCGATATGTCCAAGATCGAAAGTGGCAG
GTTAATTCTTTCGGAGGATGAGTTCAATGTGGGTGAGCTCTTGCAAGACTTGGTCGTTATGATGCAACCGGAGATCAAAA
ACAAACAACAAACTTTAAATATACACGTCAAGAACCTTAGGCATGAGAATGTAAAAGGCGATACGCAGCGTATCAAACAG
GTATTGATGAATATCCTCTCGAACGCAATTAAGTATACGCCGGAGAATGGGCGAATCACGATAGAAATCTACGAGAAGGA
CCCTCATAACGGCATCGGAAATTATCAATTCGTGTTTGAGGATAACGGAAGAGGTATGAAACCGGAGTTTCTGGATAAGA
TTTTCGAGCCTTTCGAGCGTGCGAGCGATGATGAGATCAAGAGGATACAAGGCACTGGCCTCGGGATGTCGATCAGCCAT
AAGATCATTCAGATGATGGGAGGAGATATCAAGGTGGAGAGTGAATATGGCAAAGGTTCCCGCTTCACCATTGACATGCC
ATTGGTTTGTCGGGATCAGAAGCCCGATGATAAGATAGAGGTAGAAGGTTTAGAGGTTCTTGTGGTAGATGATGATAAGA
TCGCTTGTCTAAACACGTCTAGCTGTTTGCGAGAGATCGGGATAAACAGTGAATGCGTATATTCAGGTAGCGAAGCTATA
GAAAAAGTACGACAGCATCATTTAGCGGAGAAAGAGTATTTCGCCGTGATCATTGACTTGAAGATGCCTCAAATGAACGG
CATTGAAACAACCCGGCAAATCAGACGTTTCGTGGGAGCGGACGTACCGATCATCATTCTCTCCGCATACGATCTTGAGG
AATATGAGGCAGAGGCTAAGGAGGTTAAGGCCAATGGATTCATCACCAAGCCTCTCTATAAGTCCAAGCTACTCCAAGTA
TTGAGATCTTTCCTTGACGAAGGAGACCAGCCGGAACCGATCCGCCCATTCAAGTTATCAAACGTCGATTATTCCGGCAA
GCGCATCTTGCTGGTGGAGGATAACGAGTTAAATCGTGAGATCGCCGTAGAGATCATCGGCAGCACCGGTATCACTATCG
ATACGGCGATAAATGGGCTCGACGCCGTACATAAAGTCGCCCAATCGGAAGAAGGATTTTACCAGATAATACTGATGGAT
ATCCAGATGCCTATCATGGACGGTTATGAGGCGACCCGGCAGATTCGTTCCCTCCAACGAAGGGATATCGCACATATGCC
AATTATAGCCATGACCGCCAACGCTTTTTCCGAAGACGTGACAAACGCTATCAAAGCGGGCATGAATTATCATTTGGCTA
AACCGATTGATATTGGGGCGTTGATGGGCATCCTTAGTAAATATTTACAAGCGCCCGCATAA

Upstream 100 bases:

>100_bases
ACTAGCCGAAAGCAAAAACGGAACATATTATCAAAAAGAAGAATTTCAAGGGTACCTTTTATAATTTTTACTTTTGTAGA
ATAATATAAAAAGACATACG

Downstream 100 bases:

>100_bases
AGTTTATCTTGCGTATTAAAAGTTCATGTAATAAAATTGTCTATTTTTAGCGATCAATTCGATTATAAGCATAAAGAGAC
TTCATGAACATCAGACTTCG

Product: two-component hybrid sensor kinase/response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 793; Mature: 793

Protein sequence:

>793_residues
MEGRETDLEYNLLMGLTGASVSKHLLDEHFTLLWANDFYYDMIGYPKEEYEALFHNKPDLYFKGHDEAWNILSKNVYETI
ANKASGYETVIQMPTRKGKRWTKITSTFTDQLQDGIPISYTVMTDVEDVMRRRIEQTITYDNIPGFISKHKVHKDGSFTL
LEANDKYMDFSGIDKNSFLSFSPFSRLDETSRNTMNAHIPCMLKGEPVHFVIQSKDKNGNGAWLQLNGECIGWEGDEPIY
LIVYINITDITEQRELQKKLEKQSKQLKEALKSAEQANQAKSDFLARMSHDIRTPMNAIMGMATIAKAHVDERERILDCM
EKINGASKLLLSLINEVLDMSKIESGRLILSEDEFNVGELLQDLVVMMQPEIKNKQQTLNIHVKNLRHENVKGDTQRIKQ
VLMNILSNAIKYTPENGRITIEIYEKDPHNGIGNYQFVFEDNGRGMKPEFLDKIFEPFERASDDEIKRIQGTGLGMSISH
KIIQMMGGDIKVESEYGKGSRFTIDMPLVCRDQKPDDKIEVEGLEVLVVDDDKIACLNTSSCLREIGINSECVYSGSEAI
EKVRQHHLAEKEYFAVIIDLKMPQMNGIETTRQIRRFVGADVPIIILSAYDLEEYEAEAKEVKANGFITKPLYKSKLLQV
LRSFLDEGDQPEPIRPFKLSNVDYSGKRILLVEDNELNREIAVEIIGSTGITIDTAINGLDAVHKVAQSEEGFYQIILMD
IQMPIMDGYEATRQIRSLQRRDIAHMPIIAMTANAFSEDVTNAIKAGMNYHLAKPIDIGALMGILSKYLQAPA

Sequences:

>Translated_793_residues
MEGRETDLEYNLLMGLTGASVSKHLLDEHFTLLWANDFYYDMIGYPKEEYEALFHNKPDLYFKGHDEAWNILSKNVYETI
ANKASGYETVIQMPTRKGKRWTKITSTFTDQLQDGIPISYTVMTDVEDVMRRRIEQTITYDNIPGFISKHKVHKDGSFTL
LEANDKYMDFSGIDKNSFLSFSPFSRLDETSRNTMNAHIPCMLKGEPVHFVIQSKDKNGNGAWLQLNGECIGWEGDEPIY
LIVYINITDITEQRELQKKLEKQSKQLKEALKSAEQANQAKSDFLARMSHDIRTPMNAIMGMATIAKAHVDERERILDCM
EKINGASKLLLSLINEVLDMSKIESGRLILSEDEFNVGELLQDLVVMMQPEIKNKQQTLNIHVKNLRHENVKGDTQRIKQ
VLMNILSNAIKYTPENGRITIEIYEKDPHNGIGNYQFVFEDNGRGMKPEFLDKIFEPFERASDDEIKRIQGTGLGMSISH
KIIQMMGGDIKVESEYGKGSRFTIDMPLVCRDQKPDDKIEVEGLEVLVVDDDKIACLNTSSCLREIGINSECVYSGSEAI
EKVRQHHLAEKEYFAVIIDLKMPQMNGIETTRQIRRFVGADVPIIILSAYDLEEYEAEAKEVKANGFITKPLYKSKLLQV
LRSFLDEGDQPEPIRPFKLSNVDYSGKRILLVEDNELNREIAVEIIGSTGITIDTAINGLDAVHKVAQSEEGFYQIILMD
IQMPIMDGYEATRQIRSLQRRDIAHMPIIAMTANAFSEDVTNAIKAGMNYHLAKPIDIGALMGILSKYLQAPA
>Mature_793_residues
MEGRETDLEYNLLMGLTGASVSKHLLDEHFTLLWANDFYYDMIGYPKEEYEALFHNKPDLYFKGHDEAWNILSKNVYETI
ANKASGYETVIQMPTRKGKRWTKITSTFTDQLQDGIPISYTVMTDVEDVMRRRIEQTITYDNIPGFISKHKVHKDGSFTL
LEANDKYMDFSGIDKNSFLSFSPFSRLDETSRNTMNAHIPCMLKGEPVHFVIQSKDKNGNGAWLQLNGECIGWEGDEPIY
LIVYINITDITEQRELQKKLEKQSKQLKEALKSAEQANQAKSDFLARMSHDIRTPMNAIMGMATIAKAHVDERERILDCM
EKINGASKLLLSLINEVLDMSKIESGRLILSEDEFNVGELLQDLVVMMQPEIKNKQQTLNIHVKNLRHENVKGDTQRIKQ
VLMNILSNAIKYTPENGRITIEIYEKDPHNGIGNYQFVFEDNGRGMKPEFLDKIFEPFERASDDEIKRIQGTGLGMSISH
KIIQMMGGDIKVESEYGKGSRFTIDMPLVCRDQKPDDKIEVEGLEVLVVDDDKIACLNTSSCLREIGINSECVYSGSEAI
EKVRQHHLAEKEYFAVIIDLKMPQMNGIETTRQIRRFVGADVPIIILSAYDLEEYEAEAKEVKANGFITKPLYKSKLLQV
LRSFLDEGDQPEPIRPFKLSNVDYSGKRILLVEDNELNREIAVEIIGSTGITIDTAINGLDAVHKVAQSEEGFYQIILMD
IQMPIMDGYEATRQIRSLQRRDIAHMPIIAMTANAFSEDVTNAIKAGMNYHLAKPIDIGALMGILSKYLQAPA

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=538, Percent_Identity=31.0408921933086, Blast_Score=202, Evalue=6e-53,
Organism=Escherichia coli, GI1789149, Length=548, Percent_Identity=28.2846715328467, Blast_Score=192, Evalue=7e-50,
Organism=Escherichia coli, GI1788713, Length=476, Percent_Identity=29.4117647058824, Blast_Score=162, Evalue=9e-41,
Organism=Escherichia coli, GI87081816, Length=385, Percent_Identity=30.9090909090909, Blast_Score=158, Evalue=2e-39,
Organism=Escherichia coli, GI48994928, Length=413, Percent_Identity=27.8450363196126, Blast_Score=157, Evalue=3e-39,
Organism=Escherichia coli, GI1786600, Length=249, Percent_Identity=27.710843373494, Blast_Score=105, Evalue=2e-23,
Organism=Escherichia coli, GI1786912, Length=258, Percent_Identity=26.3565891472868, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1788549, Length=288, Percent_Identity=25.6944444444444, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1790436, Length=239, Percent_Identity=26.3598326359833, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1786783, Length=249, Percent_Identity=22.0883534136546, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1788393, Length=253, Percent_Identity=23.3201581027668, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1790346, Length=240, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI1787894, Length=182, Percent_Identity=26.3736263736264, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1790437, Length=111, Percent_Identity=34.2342342342342, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=128, Percent_Identity=37.5, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6322000, Length=125, Percent_Identity=36, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 90165; Mature: 90165

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGRETDLEYNLLMGLTGASVSKHLLDEHFTLLWANDFYYDMIGYPKEEYEALFHNKPDL
CCCCCCCCEEEEEEEECCCHHHHHHHHCCEEEEEECCEEEHHCCCCHHHHHHHHCCCCCE
YFKGHDEAWNILSKNVYETIANKASGYETVIQMPTRKGKRWTKITSTFTDQLQDGIPISY
EECCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCEEE
TVMTDVEDVMRRRIEQTITYDNIPGFISKHKVHKDGSFTLLEANDKYMDFSGIDKNSFLS
EECCCHHHHHHHHHHHHCCCCCCCCCHHHCEECCCCCEEEEECCCCEECCCCCCCCCCCC
FSPFSRLDETSRNTMNAHIPCMLKGEPVHFVIQSKDKNGNGAWLQLNGECIGWEGDEPIY
CCCHHHHHHHCCCCCCCCCCEEECCCCEEEEEEECCCCCCEEEEEECCEEECCCCCCCEE
LIVYINITDITEQRELQKKLEKQSKQLKEALKSAEQANQAKSDFLARMSHDIRTPMNAIM
EEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMATIAKAHVDERERILDCMEKINGASKLLLSLINEVLDMSKIESGRLILSEDEFNVGEL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHH
LQDLVVMMQPEIKNKQQTLNIHVKNLRHENVKGDTQRIKQVLMNILSNAIKYTPENGRIT
HHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCEEE
IEIYEKDPHNGIGNYQFVFEDNGRGMKPEFLDKIFEPFERASDDEIKRIQGTGLGMSISH
EEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHH
KIIQMMGGDIKVESEYGKGSRFTIDMPLVCRDQKPDDKIEVEGLEVLVVDDDKIACLNTS
HHHHHHCCCEEEECCCCCCCEEEEECCEEECCCCCCCCEEECCEEEEEECCCCEEEECHH
SCLREIGINSECVYSGSEAIEKVRQHHLAEKEYFAVIIDLKMPQMNGIETTRQIRRFVGA
HHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCC
DVPIIILSAYDLEEYEAEAKEVKANGFITKPLYKSKLLQVLRSFLDEGDQPEPIRPFKLS
CCCEEEEEECCCHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHCCCCCCCCCCCEEEC
NVDYSGKRILLVEDNELNREIAVEIIGSTGITIDTAINGLDAVHKVAQSEEGFYQIILMD
CCCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHCCCCCEEEEEEEE
IQMPIMDGYEATRQIRSLQRRDIAHMPIIAMTANAFSEDVTNAIKAGMNYHLAKPIDIGA
ECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCEECCCCCHHHH
LMGILSKYLQAPA
HHHHHHHHHCCCC
>Mature Secondary Structure
MEGRETDLEYNLLMGLTGASVSKHLLDEHFTLLWANDFYYDMIGYPKEEYEALFHNKPDL
CCCCCCCCEEEEEEEECCCHHHHHHHHCCEEEEEECCEEEHHCCCCHHHHHHHHCCCCCE
YFKGHDEAWNILSKNVYETIANKASGYETVIQMPTRKGKRWTKITSTFTDQLQDGIPISY
EECCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCEEE
TVMTDVEDVMRRRIEQTITYDNIPGFISKHKVHKDGSFTLLEANDKYMDFSGIDKNSFLS
EECCCHHHHHHHHHHHHCCCCCCCCCHHHCEECCCCCEEEEECCCCEECCCCCCCCCCCC
FSPFSRLDETSRNTMNAHIPCMLKGEPVHFVIQSKDKNGNGAWLQLNGECIGWEGDEPIY
CCCHHHHHHHCCCCCCCCCCEEECCCCEEEEEEECCCCCCEEEEEECCEEECCCCCCCEE
LIVYINITDITEQRELQKKLEKQSKQLKEALKSAEQANQAKSDFLARMSHDIRTPMNAIM
EEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMATIAKAHVDERERILDCMEKINGASKLLLSLINEVLDMSKIESGRLILSEDEFNVGEL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHH
LQDLVVMMQPEIKNKQQTLNIHVKNLRHENVKGDTQRIKQVLMNILSNAIKYTPENGRIT
HHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCEEE
IEIYEKDPHNGIGNYQFVFEDNGRGMKPEFLDKIFEPFERASDDEIKRIQGTGLGMSISH
EEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHH
KIIQMMGGDIKVESEYGKGSRFTIDMPLVCRDQKPDDKIEVEGLEVLVVDDDKIACLNTS
HHHHHHCCCEEEECCCCCCCEEEEECCEEECCCCCCCCEEECCEEEEEECCCCEEEECHH
SCLREIGINSECVYSGSEAIEKVRQHHLAEKEYFAVIIDLKMPQMNGIETTRQIRRFVGA
HHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCC
DVPIIILSAYDLEEYEAEAKEVKANGFITKPLYKSKLLQVLRSFLDEGDQPEPIRPFKLS
CCCEEEEEECCCHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHCCCCCCCCCCCEEEC
NVDYSGKRILLVEDNELNREIAVEIIGSTGITIDTAINGLDAVHKVAQSEEGFYQIILMD
CCCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHCCCCCEEEEEEEE
IQMPIMDGYEATRQIRSLQRRDIAHMPIIAMTANAFSEDVTNAIKAGMNYHLAKPIDIGA
ECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCEECCCCCHHHH
LMGILSKYLQAPA
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]