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The map label for this gene is bfmBB [H]

Identifier: 150007338

GI number: 150007338

Start: 818088

End: 819422

Strand: Reverse

Name: bfmBB [H]

Synonym: BDI_0688

Alternate gene names: 150007338

Gene position: 819422-818088 (Counterclockwise)

Preceding gene: 150007339

Following gene: 150007337

Centisome position: 17.03

GC content: 48.46

Gene sequence:

>1335_bases
ATGGCTACATTTGAGATAAAGATGCCTAAGTTGGGCGAAAGTATTACAGAAGGAACCATCATCTCATGGTCGGTAAAGGT
AGGTGATACCGTAGAGGAAGATGATGTCCTTTTCGAAGTAAGCACAGCGAAAGTAAGCGCTGAGATTCCATCCCCCGTTG
AGGGAAAAGTAAAACAACTTCTTTTTAATGAGGGAGACACCGTAGCGGTCGGTACAGTCGTAGCGATCCTTGAGATCGAA
GGTGAAGGAGAGGATAACGGAGCTCAGCCGGAAACCTCGGAGGCTACTCAACCGAAAGAACAAGTCACCGCTCCCGCCTC
AGAGGAATTATCAAAGAATTCCCAAGAGGAAGATCGTTGGTATTCTCCCGTCGTATTGCAATTAGCGAAAACGGCCGGTG
TATCGCAAGATGAACTGGATCATATTCCGGGTACCGGATACCTAGGACGTCTCAGCAAGAAAGATATTCAAACGTATATC
GATCACAAAAATAAAGGTACGGAAATGCCGAAGCCGAAACAGGCTCCTGTTTCTTCCATGCAGCAAACGGCTACGAGCAC
GCCAACAATTACGGTAGCGCCGCCGACCGCTCCTTCCATGCCTATGACAGCGGCCACTCCATCCGCTCCACTCGCCCAAG
GAGACGAGGTGAGAGAAATGGATCGTGTACGTAAGATCATTGCGGACCATATGGTATTATCGAAAAAAGTATCTCCGCAT
GTGACGTCGGTTATCGAGGTAGACGTGACCCGACTGGTTAACTGGCGTAAAAAAGTGAAGGATCAATTCTTCAAGCAAGA
AGGCATCAACCTAACTTATATGCCGGCTATTACCGAAGCGACCGCCAAAGCGTTGAAAGCCTATCCATTAGTAAATTCAT
CCGTGGACGGTTACAATATCATCCTAAAGAAGCCTATTAATATAGGTATAGCGGTTTCCTTAAACGACGGAAATTTAATT
GTCCCGGTTATACACGATGCCGATAAGCTGAACCTAAGTGGCCTCGCCTCACAAATAGATACCTTGGCGGCTAAAGCCCG
TGAGAATAAGCTCGCCCCGGATAGCATCCAAGGCGGAACGTTCACGATTACGAATTTCGGAACCTTCAAGAGTCTGTTCG
GCACCCCGATCATCAATCAGCCTCAAGTAGCGATCCTAGCCGTCGGTTATATCGAGAAGAAGCCTGCGGTAGTAGAGACG
CCCGAGGGAGACACGATCGCTATCCGTCACAAGATGTACCTCTCACTCTCCTACGATCATCGTATCGTGGACGGAGCTTT
GGCGGGAGCCTTTTTACGTTCCATCGCCGACGAACTAGAGAATTGGAACGCTTAA

Upstream 100 bases:

>100_bases
AGAAACTTACCTTCTTGATCTTTGTTCTTATTATATACCGTTTGTAGAGACGGGGCACGCCCCGTCTCCCACTATATAAC
AGTAATTAAAAAATCAAAAT

Downstream 100 bases:

>100_bases
TACATTATTATAATAGAACAACATGAAAAAGTATGATATAAAAACAACAGATAAGGAGACTCTCAGGAAATGGTTTTACC
TTATGACGCTCGGAAGGGCG

Product: lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MATFEIKMPKLGESITEGTIISWSVKVGDTVEEDDVLFEVSTAKVSAEIPSPVEGKVKQLLFNEGDTVAVGTVVAILEIE
GEGEDNGAQPETSEATQPKEQVTAPASEELSKNSQEEDRWYSPVVLQLAKTAGVSQDELDHIPGTGYLGRLSKKDIQTYI
DHKNKGTEMPKPKQAPVSSMQQTATSTPTITVAPPTAPSMPMTAATPSAPLAQGDEVREMDRVRKIIADHMVLSKKVSPH
VTSVIEVDVTRLVNWRKKVKDQFFKQEGINLTYMPAITEATAKALKAYPLVNSSVDGYNIILKKPINIGIAVSLNDGNLI
VPVIHDADKLNLSGLASQIDTLAAKARENKLAPDSIQGGTFTITNFGTFKSLFGTPIINQPQVAILAVGYIEKKPAVVET
PEGDTIAIRHKMYLSLSYDHRIVDGALAGAFLRSIADELENWNA

Sequences:

>Translated_444_residues
MATFEIKMPKLGESITEGTIISWSVKVGDTVEEDDVLFEVSTAKVSAEIPSPVEGKVKQLLFNEGDTVAVGTVVAILEIE
GEGEDNGAQPETSEATQPKEQVTAPASEELSKNSQEEDRWYSPVVLQLAKTAGVSQDELDHIPGTGYLGRLSKKDIQTYI
DHKNKGTEMPKPKQAPVSSMQQTATSTPTITVAPPTAPSMPMTAATPSAPLAQGDEVREMDRVRKIIADHMVLSKKVSPH
VTSVIEVDVTRLVNWRKKVKDQFFKQEGINLTYMPAITEATAKALKAYPLVNSSVDGYNIILKKPINIGIAVSLNDGNLI
VPVIHDADKLNLSGLASQIDTLAAKARENKLAPDSIQGGTFTITNFGTFKSLFGTPIINQPQVAILAVGYIEKKPAVVET
PEGDTIAIRHKMYLSLSYDHRIVDGALAGAFLRSIADELENWNA
>Mature_443_residues
ATFEIKMPKLGESITEGTIISWSVKVGDTVEEDDVLFEVSTAKVSAEIPSPVEGKVKQLLFNEGDTVAVGTVVAILEIEG
EGEDNGAQPETSEATQPKEQVTAPASEELSKNSQEEDRWYSPVVLQLAKTAGVSQDELDHIPGTGYLGRLSKKDIQTYID
HKNKGTEMPKPKQAPVSSMQQTATSTPTITVAPPTAPSMPMTAATPSAPLAQGDEVREMDRVRKIIADHMVLSKKVSPHV
TSVIEVDVTRLVNWRKKVKDQFFKQEGINLTYMPAITEATAKALKAYPLVNSSVDGYNIILKKPINIGIAVSLNDGNLIV
PVIHDADKLNLSGLASQIDTLAAKARENKLAPDSIQGGTFTITNFGTFKSLFGTPIINQPQVAILAVGYIEKKPAVVETP
EGDTIAIRHKMYLSLSYDHRIVDGALAGAFLRSIADELENWNA

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=254, Percent_Identity=37.4015748031496, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI110671329, Length=443, Percent_Identity=29.7968397291196, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI31711992, Length=459, Percent_Identity=28.1045751633987, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI203098753, Length=465, Percent_Identity=28.8172043010753, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI203098816, Length=465, Percent_Identity=28.3870967741935, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI260898739, Length=154, Percent_Identity=38.3116883116883, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786946, Length=443, Percent_Identity=32.0541760722348, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1786305, Length=449, Percent_Identity=31.6258351893096, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI25146366, Length=439, Percent_Identity=31.4350797266515, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=439, Percent_Identity=28.7015945330296, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17560088, Length=455, Percent_Identity=28.7912087912088, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=223, Percent_Identity=30.4932735426009, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6320352, Length=441, Percent_Identity=31.9727891156463, Blast_Score=199, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6324258, Length=227, Percent_Identity=29.0748898678414, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24645909, Length=223, Percent_Identity=38.5650224215247, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI18859875, Length=445, Percent_Identity=29.438202247191, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24582497, Length=220, Percent_Identity=33.1818181818182, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI20129315, Length=220, Percent_Identity=33.1818181818182, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 48012; Mature: 47881

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATFEIKMPKLGESITEGTIISWSVKVGDTVEEDDVLFEVSTAKVSAEIPSPVEGKVKQL
CCEEEEECCHHCCCCCCCEEEEEEEEECCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHH
LFNEGDTVAVGTVVAILEIEGEGEDNGAQPETSEATQPKEQVTAPASEELSKNSQEEDRW
HHCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHCCCCHHHHH
YSPVVLQLAKTAGVSQDELDHIPGTGYLGRLSKKDIQTYIDHKNKGTEMPKPKQAPVSSM
CCHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHH
QQTATSTPTITVAPPTAPSMPMTAATPSAPLAQGDEVREMDRVRKIIADHMVLSKKVSPH
HHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
VTSVIEVDVTRLVNWRKKVKDQFFKQEGINLTYMPAITEATAKALKAYPLVNSSVDGYNI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCEEE
ILKKPINIGIAVSLNDGNLIVPVIHDADKLNLSGLASQIDTLAAKARENKLAPDSIQGGT
EEECCCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
FTITNFGTFKSLFGTPIINQPQVAILAVGYIEKKPAVVETPEGDTIAIRHKMYLSLSYDH
EEEECCCHHHHHHCCCCCCCCCEEEEEEEEECCCCCEEECCCCCEEEEEEEEEEEEECCC
RIVDGALAGAFLRSIADELENWNA
CHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ATFEIKMPKLGESITEGTIISWSVKVGDTVEEDDVLFEVSTAKVSAEIPSPVEGKVKQL
CEEEEECCHHCCCCCCCEEEEEEEEECCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHH
LFNEGDTVAVGTVVAILEIEGEGEDNGAQPETSEATQPKEQVTAPASEELSKNSQEEDRW
HHCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHCCCCHHHHH
YSPVVLQLAKTAGVSQDELDHIPGTGYLGRLSKKDIQTYIDHKNKGTEMPKPKQAPVSSM
CCHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHH
QQTATSTPTITVAPPTAPSMPMTAATPSAPLAQGDEVREMDRVRKIIADHMVLSKKVSPH
HHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
VTSVIEVDVTRLVNWRKKVKDQFFKQEGINLTYMPAITEATAKALKAYPLVNSSVDGYNI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCEEE
ILKKPINIGIAVSLNDGNLIVPVIHDADKLNLSGLASQIDTLAAKARENKLAPDSIQGGT
EEECCCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
FTITNFGTFKSLFGTPIINQPQVAILAVGYIEKKPAVVETPEGDTIAIRHKMYLSLSYDH
EEEECCCHHHHHHCCCCCCCCCEEEEEEEEECCCCCEEECCCCCEEEEEEEEEEEEECCC
RIVDGALAGAFLRSIADELENWNA
CHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]