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The map label for this gene is bkdA2 [H]

Identifier: 150007337

GI number: 150007337

Start: 816032

End: 818065

Strand: Reverse

Name: bkdA2 [H]

Synonym: BDI_0687

Alternate gene names: 150007337

Gene position: 818065-816032 (Counterclockwise)

Preceding gene: 150007338

Following gene: 150007336

Centisome position: 17.0

GC content: 50.84

Gene sequence:

>2034_bases
ATGAAAAAGTATGATATAAAAACAACAGATAAGGAGACTCTCAGGAAATGGTTTTACCTTATGACGCTCGGAAGGGCGAT
CGATGAGAAGGCTCCCTCCTATTTATTGCAATCTCTCGGCTGGTCTTATCATGCCCCGTATGCCGGGCACGACGGGATAC
AACTCGCCATGGGACAAGTATTCGATAAGGATACGGACTTTCTCTTCCCCTATTACCGGGATATGTTGACGGTTCTCTCG
GCGGGAATGACCGCCGAGGAGATCATCCTGAACGGTATCTCCAAGGCGACCGATCTGACCAGTGGCGGCCGCCACATGTC
TAACCACTTCTCTAAGATGGAATGGCATATCGAGAACGTTTCTTCCGCTACGGCGACTCACGATCTCCATGCCGCCGGCG
TGGCACGGGCGATGGTTTACTATGGACAGAAAGGCGTAGCGATTACCTCGCACGGTGAATCGGCCGCTTCCGAAGGCTAT
GTGTATGAGGCAATCAATGGCGCCAGCAATGAGCGACTGCCGGTTATCTTTGTCTTCCAAGACAATGGATATGGTATCTC
TGTCCCCAAAAAGGACCAGACGGCCAATCGTAAAGTTGCCGATAACTTCTCTGGCTTCAAGAATTTACGTATTATCCATT
GCAATGGAAAAGACGTGTTCGATTCCATGAATGCCATGACCGAGGCCAAAGAGTACGCGATCGCCAACCGGACTCCCGTA
ATCGTTCAGGCCAATTGCGTACGGATAGGCTCCCACTCAAACTCCGATAAACATACCTTGTATCGTGATGAGAACGAGCT
TACCTATGTAAAATCCGCCGATCCTTTATACAAATTCCATCGCATGCTGATTCGTTACGGACGTTTCACCGAGGAAGAAT
TGAAAGAGATCGCGGATCTAGCGGCTAAAGACCTGAAAGCCGCCAATCGGAAAGCGATGGCCGCCCCCGATCCCGATCCG
TCTACCGTCAAGGATTACGTCCTCCCGGAACCTTATCAACCTCAAAAATACAAAGAAGGTGTTCAAAACGAGGAAGGAGA
GAAAGAAACGCTAGTTACGGCGATCAACAAGACGTTGAAAGCGGAGTTCCGGCACAACCCGGATACGTTTATCTGGGGAC
AGGATGTAGCCAATAAAGAAAAGGGTGGCGTATTCAACATCACGAAAGGCATGCAGCAAGAATTCGGTATCGAGCGAGTC
TTCAACGCCCCTATCGCCGAGGATTATATCGTAGGGACAGCGAACGGTATGTGCCGCTTTGACCCGAAGATCCATGTAGT
GATCGAGGGCGCGGAATTCGCCGATTACTTCTGGCCGGCCGTCGAGCAATACGTGGAATGTACGCATGAGTATTGGCGCA
GCAACGGGCAATTTACCCCAAACATCACTTTGCGCCTAGCCTCCGGAGGCTATATCGGAGGAGGGCTTTATCATTCCCAG
ACCATTGAGGGCGCATTGACCTCATTACCCGGTGCCCGGATTGTTTATCCCTCTTTCGCGGATGATGCCGCCGGACTGTT
GCGGACGAGTATGCGTTCTAAAGGCTTTACGCTCTATCTGGAACCGAAAGCGCTCTACAACGCGGTAGAAGCCTCCACCT
TTGTCCCCGAGGATTTCGAGGTTCCGTTTGGGAAAGCCCGTATCCGCCGTCCGGGAAAAGATCTGACAATCATCACTTAT
GGCAATACGACCCATCTTTGCCTCAACGTAGCCGAGCTTCTTTACAAGGAGAAAGGCTGGGAACTGGAGGTTATCGATCT
TCGTACCTTGATACCATTGGATAAAGAGGCGATCTTCAACTCGGTGAAGAAAACCAGTAAAGTATTGATCGTACATGAGG
ACAAGGTCTTCTCTGGCTTCGGAGCGGAAATTGCCGGCATCATCGGCTCGGAACTATTCCAGTACTTGGATGCTCCGATC
CAACGGGTAGGTTCCCTATTCACTCCGGTTGGTTTCCATCCGGTCTTGGAACGGGCCATCCTCCCCAACGAGGAGACCAT
CTATCATGCGGCAAAAGAACTGCTGCTTTATTAG

Upstream 100 bases:

>100_bases
CATCGTATCGTGGACGGAGCTTTGGCGGGAGCCTTTTTACGTTCCATCGCCGACGAACTAGAGAATTGGAACGCTTAATA
CATTATTATAATAGAACAAC

Downstream 100 bases:

>100_bases
ATCCACATTCGTTAAATCTTTAAAAGCATCACAATGAAAACAGGATTATTTTACGGAACGGGCACAGAGAAAACAACCTT
TGTCGCCAATAAAATAAGAA

Product: 2-oxoisovalerate dehydrogenase subunit beta

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta [H]

Number of amino acids: Translated: 677; Mature: 677

Protein sequence:

>677_residues
MKKYDIKTTDKETLRKWFYLMTLGRAIDEKAPSYLLQSLGWSYHAPYAGHDGIQLAMGQVFDKDTDFLFPYYRDMLTVLS
AGMTAEEIILNGISKATDLTSGGRHMSNHFSKMEWHIENVSSATATHDLHAAGVARAMVYYGQKGVAITSHGESAASEGY
VYEAINGASNERLPVIFVFQDNGYGISVPKKDQTANRKVADNFSGFKNLRIIHCNGKDVFDSMNAMTEAKEYAIANRTPV
IVQANCVRIGSHSNSDKHTLYRDENELTYVKSADPLYKFHRMLIRYGRFTEEELKEIADLAAKDLKAANRKAMAAPDPDP
STVKDYVLPEPYQPQKYKEGVQNEEGEKETLVTAINKTLKAEFRHNPDTFIWGQDVANKEKGGVFNITKGMQQEFGIERV
FNAPIAEDYIVGTANGMCRFDPKIHVVIEGAEFADYFWPAVEQYVECTHEYWRSNGQFTPNITLRLASGGYIGGGLYHSQ
TIEGALTSLPGARIVYPSFADDAAGLLRTSMRSKGFTLYLEPKALYNAVEASTFVPEDFEVPFGKARIRRPGKDLTIITY
GNTTHLCLNVAELLYKEKGWELEVIDLRTLIPLDKEAIFNSVKKTSKVLIVHEDKVFSGFGAEIAGIIGSELFQYLDAPI
QRVGSLFTPVGFHPVLERAILPNEETIYHAAKELLLY

Sequences:

>Translated_677_residues
MKKYDIKTTDKETLRKWFYLMTLGRAIDEKAPSYLLQSLGWSYHAPYAGHDGIQLAMGQVFDKDTDFLFPYYRDMLTVLS
AGMTAEEIILNGISKATDLTSGGRHMSNHFSKMEWHIENVSSATATHDLHAAGVARAMVYYGQKGVAITSHGESAASEGY
VYEAINGASNERLPVIFVFQDNGYGISVPKKDQTANRKVADNFSGFKNLRIIHCNGKDVFDSMNAMTEAKEYAIANRTPV
IVQANCVRIGSHSNSDKHTLYRDENELTYVKSADPLYKFHRMLIRYGRFTEEELKEIADLAAKDLKAANRKAMAAPDPDP
STVKDYVLPEPYQPQKYKEGVQNEEGEKETLVTAINKTLKAEFRHNPDTFIWGQDVANKEKGGVFNITKGMQQEFGIERV
FNAPIAEDYIVGTANGMCRFDPKIHVVIEGAEFADYFWPAVEQYVECTHEYWRSNGQFTPNITLRLASGGYIGGGLYHSQ
TIEGALTSLPGARIVYPSFADDAAGLLRTSMRSKGFTLYLEPKALYNAVEASTFVPEDFEVPFGKARIRRPGKDLTIITY
GNTTHLCLNVAELLYKEKGWELEVIDLRTLIPLDKEAIFNSVKKTSKVLIVHEDKVFSGFGAEIAGIIGSELFQYLDAPI
QRVGSLFTPVGFHPVLERAILPNEETIYHAAKELLLY
>Mature_677_residues
MKKYDIKTTDKETLRKWFYLMTLGRAIDEKAPSYLLQSLGWSYHAPYAGHDGIQLAMGQVFDKDTDFLFPYYRDMLTVLS
AGMTAEEIILNGISKATDLTSGGRHMSNHFSKMEWHIENVSSATATHDLHAAGVARAMVYYGQKGVAITSHGESAASEGY
VYEAINGASNERLPVIFVFQDNGYGISVPKKDQTANRKVADNFSGFKNLRIIHCNGKDVFDSMNAMTEAKEYAIANRTPV
IVQANCVRIGSHSNSDKHTLYRDENELTYVKSADPLYKFHRMLIRYGRFTEEELKEIADLAAKDLKAANRKAMAAPDPDP
STVKDYVLPEPYQPQKYKEGVQNEEGEKETLVTAINKTLKAEFRHNPDTFIWGQDVANKEKGGVFNITKGMQQEFGIERV
FNAPIAEDYIVGTANGMCRFDPKIHVVIEGAEFADYFWPAVEQYVECTHEYWRSNGQFTPNITLRLASGGYIGGGLYHSQ
TIEGALTSLPGARIVYPSFADDAAGLLRTSMRSKGFTLYLEPKALYNAVEASTFVPEDFEVPFGKARIRRPGKDLTIITY
GNTTHLCLNVAELLYKEKGWELEVIDLRTLIPLDKEAIFNSVKKTSKVLIVHEDKVFSGFGAEIAGIIGSELFQYLDAPI
QRVGSLFTPVGFHPVLERAILPNEETIYHAAKELLLY

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=352, Percent_Identity=34.375, Blast_Score=188, Evalue=2e-47,
Organism=Homo sapiens, GI34101272, Length=352, Percent_Identity=34.375, Blast_Score=188, Evalue=2e-47,
Organism=Homo sapiens, GI156564403, Length=334, Percent_Identity=30.8383233532934, Blast_Score=145, Evalue=2e-34,
Organism=Homo sapiens, GI291084858, Length=333, Percent_Identity=29.4294294294294, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI11386135, Length=334, Percent_Identity=30.2395209580838, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI258645172, Length=335, Percent_Identity=30.1492537313433, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI4505685, Length=304, Percent_Identity=25, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI291084742, Length=304, Percent_Identity=25, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI291084744, Length=188, Percent_Identity=29.7872340425532, Blast_Score=87, Evalue=7e-17,
Organism=Homo sapiens, GI4885543, Length=306, Percent_Identity=23.8562091503268, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI291084757, Length=180, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17506935, Length=350, Percent_Identity=34.8571428571429, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17538422, Length=340, Percent_Identity=31.4705882352941, Blast_Score=156, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI86563355, Length=257, Percent_Identity=31.5175097276265, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI86563357, Length=257, Percent_Identity=31.5175097276265, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI32564172, Length=241, Percent_Identity=29.8755186721992, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17536047, Length=241, Percent_Identity=29.8755186721992, Blast_Score=94, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319698, Length=331, Percent_Identity=34.1389728096677, Blast_Score=170, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6321026, Length=190, Percent_Identity=29.4736842105263, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI160714832, Length=333, Percent_Identity=34.8348348348348, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI160714828, Length=333, Percent_Identity=34.8348348348348, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI21358145, Length=336, Percent_Identity=30.952380952381, Blast_Score=149, Evalue=8e-36,
Organism=Drosophila melanogaster, GI24650940, Length=336, Percent_Identity=30.952380952381, Blast_Score=149, Evalue=8e-36,
Organism=Drosophila melanogaster, GI21355903, Length=252, Percent_Identity=29.3650793650794, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24639740, Length=302, Percent_Identity=27.1523178807947, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24639744, Length=302, Percent_Identity=27.1523178807947, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28571106, Length=302, Percent_Identity=27.1523178807947, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24639746, Length=260, Percent_Identity=29.2307692307692, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24639748, Length=311, Percent_Identity=23.7942122186495, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 75815; Mature: 75815

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYDIKTTDKETLRKWFYLMTLGRAIDEKAPSYLLQSLGWSYHAPYAGHDGIQLAMGQV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
FDKDTDFLFPYYRDMLTVLSAGMTAEEIILNGISKATDLTSGGRHMSNHFSKMEWHIENV
HCCCCCEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
SSATATHDLHAAGVARAMVYYGQKGVAITSHGESAASEGYVYEAINGASNERLPVIFVFQ
CCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCEEEEEEE
DNGYGISVPKKDQTANRKVADNFSGFKNLRIIHCNGKDVFDSMNAMTEAKEYAIANRTPV
CCCEEECCCCCCCCCCCHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCE
IVQANCVRIGSHSNSDKHTLYRDENELTYVKSADPLYKFHRMLIRYGRFTEEELKEIADL
EEEECEEEECCCCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHH
AAKDLKAANRKAMAAPDPDPSTVKDYVLPEPYQPQKYKEGVQNEEGEKETLVTAINKTLK
HHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHH
AEFRHNPDTFIWGQDVANKEKGGVFNITKGMQQEFGIERVFNAPIAEDYIVGTANGMCRF
HHHCCCCCCEEECCCCCCCCCCCEEEEHHHHHHHHCHHHHHCCCCCCCEEEECCCCCEEE
DPKIHVVIEGAEFADYFWPAVEQYVECTHEYWRSNGQFTPNITLRLASGGYIGGGLYHSQ
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEECCCCCHHH
TIEGALTSLPGARIVYPSFADDAAGLLRTSMRSKGFTLYLEPKALYNAVEASTFVPEDFE
HHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCCCCCCC
VPFGKARIRRPGKDLTIITYGNTTHLCLNVAELLYKEKGWELEVIDLRTLIPLDKEAIFN
CCCCHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCHHHHHH
SVKKTSKVLIVHEDKVFSGFGAEIAGIIGSELFQYLDAPIQRVGSLFTPVGFHPVLERAI
HHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHC
LPNEETIYHAAKELLLY
CCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKYDIKTTDKETLRKWFYLMTLGRAIDEKAPSYLLQSLGWSYHAPYAGHDGIQLAMGQV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
FDKDTDFLFPYYRDMLTVLSAGMTAEEIILNGISKATDLTSGGRHMSNHFSKMEWHIENV
HCCCCCEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
SSATATHDLHAAGVARAMVYYGQKGVAITSHGESAASEGYVYEAINGASNERLPVIFVFQ
CCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCEEEEEEE
DNGYGISVPKKDQTANRKVADNFSGFKNLRIIHCNGKDVFDSMNAMTEAKEYAIANRTPV
CCCEEECCCCCCCCCCCHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCE
IVQANCVRIGSHSNSDKHTLYRDENELTYVKSADPLYKFHRMLIRYGRFTEEELKEIADL
EEEECEEEECCCCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHH
AAKDLKAANRKAMAAPDPDPSTVKDYVLPEPYQPQKYKEGVQNEEGEKETLVTAINKTLK
HHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHH
AEFRHNPDTFIWGQDVANKEKGGVFNITKGMQQEFGIERVFNAPIAEDYIVGTANGMCRF
HHHCCCCCCEEECCCCCCCCCCCEEEEHHHHHHHHCHHHHHCCCCCCCEEEECCCCCEEE
DPKIHVVIEGAEFADYFWPAVEQYVECTHEYWRSNGQFTPNITLRLASGGYIGGGLYHSQ
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEECCCCCHHH
TIEGALTSLPGARIVYPSFADDAAGLLRTSMRSKGFTLYLEPKALYNAVEASTFVPEDFE
HHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCCCCCCC
VPFGKARIRRPGKDLTIITYGNTTHLCLNVAELLYKEKGWELEVIDLRTLIPLDKEAIFN
CCCCHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCHHHHHH
SVKKTSKVLIVHEDKVFSGFGAEIAGIIGSELFQYLDAPIQRVGSLFTPVGFHPVLERAI
HHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHC
LPNEETIYHAAKELLLY
CCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3416875; 10426958 [H]