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The map label for this gene is pgk [H]

Identifier: 150007325

GI number: 150007325

Start: 799507

End: 800778

Strand: Reverse

Name: pgk [H]

Synonym: BDI_0672

Alternate gene names: 150007325

Gene position: 800778-799507 (Counterclockwise)

Preceding gene: 150007326

Following gene: 150007323

Centisome position: 16.64

GC content: 47.25

Gene sequence:

>1272_bases
ATGCAAACAATTGACAATTTCAATTTTGCCGGCAAAAAGGCATTTGTAAGAGTTGACTTTAACGTTCCATTGGACGAAAA
CTTCAACATCACCGACGACACTCGTATGCGTGCCGCTCTTCCTACATTAAAGAAGATCTTAGCGGATGGTGGTAGCGTAA
TTATCGGTTCTCACCTAGGTCGTCCGAAAGGCGCTACTGATAAGTTCTCCTTAAAGCACATATTGAAGCACCTTTCTGAG
TTGTTAGGCGTAGATGTTCAGTTTGCAAACGACTGTATTGGCGAAGAAGCCGGCGCTAAGGCTGCCGCTTTGCAACCGGG
CGAAGCTTTGTTGCTTGAGAACCTTCGTTTCTACGCTGAGGAAGAAGGCAAACCAAGAGGTTTGGCTGAAGACGCTTCCG
ACGAGGAGAAGAAGGCTGCCAAGAAAGCCGTTAAAGAAAGCCAGAAAGAGTTCACGAAGAGATTGGCCTCTTACGCTGAC
TGTTATGTAAACGACGCATTCGGTACCGCTCACCGTGCTCACGCTTCTACGGCTTTGATCGCTGACTACTTCGATACGGA
CAACAAGATGTTCGGTTATTTGATGGAGAAAGAAGTGAAGGCTGTCGATAAGGTTATGAACGACATCCAACGTCCGTTCA
CGGCTATCATGGGTGGTTCTAAGGTTTCCTCTAAGATCGAGATCATCGAGAACTTGTTGAACAAGGTAGACAACTTGATC
ATCACGGGTGGTATGACTTATACTTTCACGAAAGCGCAAGGCGGTAAGATCGGTAAGTCTATCTGCGAGGACGATAAGTT
GGATCTTGCTCTTGATTTGATCAAGAAAGCGAAAGAAAAAGGCGTAAACTTGGTATTGGCTGTTGACGCTAAGATCGCTG
ACGATTTCAGCAACGACGCTAAGACTGATTTCGCTCCGGTAGATCAGATTCCTGATGGTTGGGAAGGTCTTGACATAGGT
CCGAAGACCGAGGCTATCTACGCTGACGTTATCAAGAACTCTAAGACGATCCTTTGGAACGGTCCTACGGGTGTATTCGA
GTTCGAGAACTTTACGCACGGATCTCGCACGGTTGGCGAGGCTATCGTTGAGGCTACGAAGAACGGCGCTTTCTCTTTAG
TAGGTGGTGGCGACTCTGTTGCTTGCGTTAACAAATTCGGTTTGGCTAACGGCGTATCTTACGTTTCTACAGGTGGTGGC
GCTCTTCTTGAGGCTATCGAAGGTAAGGTTCTTCCGGGTATCGCAGCTGTAAAAGGCGAGGCTTACAAATAA

Upstream 100 bases:

>100_bases
TAAAATTTAAAGCAAGCGCAAACGGTCAAATAAAAATATTTTATGTAAGTTTGCAAGCGATTAAAGGAACATTTTAATAC
AGACATTAAATACAATAGTT

Downstream 100 bases:

>100_bases
TCAGATAAGTAAGTGATTTAATAAAAAATCCCCCGGGACGCTCGGTATTCATCGAGATGTCCCGGGGGATTTTCTTTGAT
TTAGTTCCGTATTAATCTTG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MQTIDNFNFAGKKAFVRVDFNVPLDENFNITDDTRMRAALPTLKKILADGGSVIIGSHLGRPKGATDKFSLKHILKHLSE
LLGVDVQFANDCIGEEAGAKAAALQPGEALLLENLRFYAEEEGKPRGLAEDASDEEKKAAKKAVKESQKEFTKRLASYAD
CYVNDAFGTAHRAHASTALIADYFDTDNKMFGYLMEKEVKAVDKVMNDIQRPFTAIMGGSKVSSKIEIIENLLNKVDNLI
ITGGMTYTFTKAQGGKIGKSICEDDKLDLALDLIKKAKEKGVNLVLAVDAKIADDFSNDAKTDFAPVDQIPDGWEGLDIG
PKTEAIYADVIKNSKTILWNGPTGVFEFENFTHGSRTVGEAIVEATKNGAFSLVGGGDSVACVNKFGLANGVSYVSTGGG
ALLEAIEGKVLPGIAAVKGEAYK

Sequences:

>Translated_423_residues
MQTIDNFNFAGKKAFVRVDFNVPLDENFNITDDTRMRAALPTLKKILADGGSVIIGSHLGRPKGATDKFSLKHILKHLSE
LLGVDVQFANDCIGEEAGAKAAALQPGEALLLENLRFYAEEEGKPRGLAEDASDEEKKAAKKAVKESQKEFTKRLASYAD
CYVNDAFGTAHRAHASTALIADYFDTDNKMFGYLMEKEVKAVDKVMNDIQRPFTAIMGGSKVSSKIEIIENLLNKVDNLI
ITGGMTYTFTKAQGGKIGKSICEDDKLDLALDLIKKAKEKGVNLVLAVDAKIADDFSNDAKTDFAPVDQIPDGWEGLDIG
PKTEAIYADVIKNSKTILWNGPTGVFEFENFTHGSRTVGEAIVEATKNGAFSLVGGGDSVACVNKFGLANGVSYVSTGGG
ALLEAIEGKVLPGIAAVKGEAYK
>Mature_423_residues
MQTIDNFNFAGKKAFVRVDFNVPLDENFNITDDTRMRAALPTLKKILADGGSVIIGSHLGRPKGATDKFSLKHILKHLSE
LLGVDVQFANDCIGEEAGAKAAALQPGEALLLENLRFYAEEEGKPRGLAEDASDEEKKAAKKAVKESQKEFTKRLASYAD
CYVNDAFGTAHRAHASTALIADYFDTDNKMFGYLMEKEVKAVDKVMNDIQRPFTAIMGGSKVSSKIEIIENLLNKVDNLI
ITGGMTYTFTKAQGGKIGKSICEDDKLDLALDLIKKAKEKGVNLVLAVDAKIADDFSNDAKTDFAPVDQIPDGWEGLDIG
PKTEAIYADVIKNSKTILWNGPTGVFEFENFTHGSRTVGEAIVEATKNGAFSLVGGGDSVACVNKFGLANGVSYVSTGGG
ALLEAIEGKVLPGIAAVKGEAYK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=420, Percent_Identity=44.047619047619, Blast_Score=338, Evalue=6e-93,
Organism=Homo sapiens, GI31543397, Length=420, Percent_Identity=41.9047619047619, Blast_Score=326, Evalue=3e-89,
Organism=Escherichia coli, GI1789294, Length=422, Percent_Identity=37.4407582938389, Blast_Score=270, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17508823, Length=418, Percent_Identity=45.933014354067, Blast_Score=356, Evalue=1e-98,
Organism=Saccharomyces cerevisiae, GI6319857, Length=417, Percent_Identity=45.5635491606715, Blast_Score=348, Evalue=9e-97,
Organism=Drosophila melanogaster, GI17136394, Length=419, Percent_Identity=46.5393794749403, Blast_Score=340, Evalue=8e-94,
Organism=Drosophila melanogaster, GI24581203, Length=420, Percent_Identity=43.8095238095238, Blast_Score=320, Evalue=1e-87,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 45445; Mature: 45445

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTIDNFNFAGKKAFVRVDFNVPLDENFNITDDTRMRAALPTLKKILADGGSVIIGSHLG
CCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCC
RPKGATDKFSLKHILKHLSELLGVDVQFANDCIGEEAGAKAAALQPGEALLLENLRFYAE
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHC
EEGKPRGLAEDASDEEKKAAKKAVKESQKEFTKRLASYADCYVNDAFGTAHRAHASTALI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ADYFDTDNKMFGYLMEKEVKAVDKVMNDIQRPFTAIMGGSKVSSKIEIIENLLNKVDNLI
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEE
ITGGMTYTFTKAQGGKIGKSICEDDKLDLALDLIKKAKEKGVNLVLAVDAKIADDFSNDA
EECCCEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCCC
KTDFAPVDQIPDGWEGLDIGPKTEAIYADVIKNSKTILWNGPTGVFEFENFTHGSRTVGE
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCEEECCCCCCCHHHHH
AIVEATKNGAFSLVGGGDSVACVNKFGLANGVSYVSTGGGALLEAIEGKVLPGIAAVKGE
HHHHHHCCCCEEEECCCCCCHHHHHHCCCCCCCCEECCCHHHHHHHCCCCCCCHHHCCCC
AYK
CCC
>Mature Secondary Structure
MQTIDNFNFAGKKAFVRVDFNVPLDENFNITDDTRMRAALPTLKKILADGGSVIIGSHLG
CCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCC
RPKGATDKFSLKHILKHLSELLGVDVQFANDCIGEEAGAKAAALQPGEALLLENLRFYAE
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHC
EEGKPRGLAEDASDEEKKAAKKAVKESQKEFTKRLASYADCYVNDAFGTAHRAHASTALI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ADYFDTDNKMFGYLMEKEVKAVDKVMNDIQRPFTAIMGGSKVSSKIEIIENLLNKVDNLI
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEE
ITGGMTYTFTKAQGGKIGKSICEDDKLDLALDLIKKAKEKGVNLVLAVDAKIADDFSNDA
EECCCEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCCC
KTDFAPVDQIPDGWEGLDIGPKTEAIYADVIKNSKTILWNGPTGVFEFENFTHGSRTVGE
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCEEECCCCCCCHHHHH
AIVEATKNGAFSLVGGGDSVACVNKFGLANGVSYVSTGGGALLEAIEGKVLPGIAAVKGE
HHHHHHCCCCEEEECCCCCCHHHHHHCCCCCCCCEECCCHHHHHHHCCCCCCCHHHCCCC
AYK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]