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The map label for this gene is mleN [H]

Identifier: 150007265

GI number: 150007265

Start: 717501

End: 718796

Strand: Reverse

Name: mleN [H]

Synonym: BDI_0610

Alternate gene names: 150007265

Gene position: 718796-717501 (Counterclockwise)

Preceding gene: 150007266

Following gene: 150007252

Centisome position: 14.94

GC content: 55.48

Gene sequence:

>1296_bases
ATGGGGGAGTTGGGACGTAGACCGAACGCATGGGCATTGACGCCGCTGGTGGTATTCTTATTGACTTATTTGGTGGTATC
TATCGTGGCGGGAGATTTCTATAAGATGCCGATAACGGTAGCCTTCGTCATCTCCTCGATCGTAGCGATAGCGATCTCCA
AGGGAGGCAAATTAAGCAACCGTATCGAGCAGTTCTGCCGGGGGGCGGCCAACAGTAATATCATGCTGATGGTATTGATC
TTCATCCTCGCCGGGGCCTTCGCGCAGACCGCCAAGGCGATGGGTGCCGTAGACGCTACCGTGAATCTGGCGATGTCCAT
CCTGCCCGGTAACCTGTTGGCGGCCGGAATCTTCCTAGCCGCTTGCTTCATATCCATCTCGGTAGGTACGTCGGTGGGCA
CGATCGTGGCCTTGGCCCCGGTAGCCGTCGGTATAGCGGACAAGACGGGGATGCCGGACGCCCTTATGCTGGGTGTCGTG
GTGAGCGGCGCCATGTTCGGCGATAACCTGTCGTTCATATCCGATACGACTATCGTAGCTACCCGCACGCAGGGTTGCCA
GATGTCCGATAAGTTCAAGGTGAATATCCGTATCGCCCTCCCGATAGCGATCCTGACCGGATTGATATACGTGCTGGTGG
GAAGCGGCGTGGAGAACGCTTACGCCCCCGCCCCGATCGAATGGGTCAAGGTGATCCCTTATCTGGTGGTATTGGTAACC
GCCGTTTGCGGGATCAATGTCATGCTGGTGCTATTTATCGGGATCGTCCTGTCCGGTATCGTAGGTCTGTGGACCGGTAG
CTTTGATATCTGGGGCTGGAACGCCTCCATGGGACTAGGTATCACGAATATGGGCGAGCTGATTATCGTGACGCTTCTGG
CAGGAGGCATGTTGGAGATGATCCGTTATAACGGGGGTATCGACTGGATCATCCGCAAGCTTACCTCCCGCATCCATTCC
TCCAAGGGGGCCGAGATGAGCATAGCGGCCTTGGTGAGCTTCGCCAACCTCTGCACGGCCAACAATACGATCGCCCTCAT
CATGGCCGGCCCGATCGCCAAAGATATAGCGGACCGGTTCAAGATAGATCCCCGCCGCAGCGCCAGCCTATTGGATATCT
TCTCTTGCTTCGTACAGGGAATCATCCCCTACGGCGCCCAGATGTTGATGGCCGCCGGACTCGGGCATGTCTCCCCTATC
GAGATCATGCAATATCTTTACTACCCGTATCTATTGGGAGCAGGCGCCTTATTGGCGATCTTATTCAATTACCCGAGGAA
ATATACATACGTATAG

Upstream 100 bases:

>100_bases
TCAAGAAGGGGGGCGTGCGTATAGCGCTGCGGTTCTCCTTCTAAGAGAAGGGACGTAGAGAATTGACAGTTGACAGTTAA
TTTAAGAGAGATAAGAAAAA

Downstream 100 bases:

>100_bases
GCTGCCCGTCCTTACGAAAAAACACAATAGTATTCAAAGCAAAAAGACTATTGTGTCCGGGATTGAATACTATTGTGTTC
GCTGATGAATACTACTGTGT

Product: putative Na+/H+ antiporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MGELGRRPNAWALTPLVVFLLTYLVVSIVAGDFYKMPITVAFVISSIVAIAISKGGKLSNRIEQFCRGAANSNIMLMVLI
FILAGAFAQTAKAMGAVDATVNLAMSILPGNLLAAGIFLAACFISISVGTSVGTIVALAPVAVGIADKTGMPDALMLGVV
VSGAMFGDNLSFISDTTIVATRTQGCQMSDKFKVNIRIALPIAILTGLIYVLVGSGVENAYAPAPIEWVKVIPYLVVLVT
AVCGINVMLVLFIGIVLSGIVGLWTGSFDIWGWNASMGLGITNMGELIIVTLLAGGMLEMIRYNGGIDWIIRKLTSRIHS
SKGAEMSIAALVSFANLCTANNTIALIMAGPIAKDIADRFKIDPRRSASLLDIFSCFVQGIIPYGAQMLMAAGLGHVSPI
EIMQYLYYPYLLGAGALLAILFNYPRKYTYV

Sequences:

>Translated_431_residues
MGELGRRPNAWALTPLVVFLLTYLVVSIVAGDFYKMPITVAFVISSIVAIAISKGGKLSNRIEQFCRGAANSNIMLMVLI
FILAGAFAQTAKAMGAVDATVNLAMSILPGNLLAAGIFLAACFISISVGTSVGTIVALAPVAVGIADKTGMPDALMLGVV
VSGAMFGDNLSFISDTTIVATRTQGCQMSDKFKVNIRIALPIAILTGLIYVLVGSGVENAYAPAPIEWVKVIPYLVVLVT
AVCGINVMLVLFIGIVLSGIVGLWTGSFDIWGWNASMGLGITNMGELIIVTLLAGGMLEMIRYNGGIDWIIRKLTSRIHS
SKGAEMSIAALVSFANLCTANNTIALIMAGPIAKDIADRFKIDPRRSASLLDIFSCFVQGIIPYGAQMLMAAGLGHVSPI
EIMQYLYYPYLLGAGALLAILFNYPRKYTYV
>Mature_430_residues
GELGRRPNAWALTPLVVFLLTYLVVSIVAGDFYKMPITVAFVISSIVAIAISKGGKLSNRIEQFCRGAANSNIMLMVLIF
ILAGAFAQTAKAMGAVDATVNLAMSILPGNLLAAGIFLAACFISISVGTSVGTIVALAPVAVGIADKTGMPDALMLGVVV
SGAMFGDNLSFISDTTIVATRTQGCQMSDKFKVNIRIALPIAILTGLIYVLVGSGVENAYAPAPIEWVKVIPYLVVLVTA
VCGINVMLVLFIGIVLSGIVGLWTGSFDIWGWNASMGLGITNMGELIIVTLLAGGMLEMIRYNGGIDWIIRKLTSRIHSS
KGAEMSIAALVSFANLCTANNTIALIMAGPIAKDIADRFKIDPRRSASLLDIFSCFVQGIIPYGAQMLMAAGLGHVSPIE
IMQYLYYPYLLGAGALLAILFNYPRKYTYV

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 45654; Mature: 45523

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGELGRRPNAWALTPLVVFLLTYLVVSIVAGDFYKMPITVAFVISSIVAIAISKGGKLSN
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RIEQFCRGAANSNIMLMVLIFILAGAFAQTAKAMGAVDATVNLAMSILPGNLLAAGIFLA
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ACFISISVGTSVGTIVALAPVAVGIADKTGMPDALMLGVVVSGAMFGDNLSFISDTTIVA
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCEEEE
TRTQGCQMSDKFKVNIRIALPIAILTGLIYVLVGSGVENAYAPAPIEWVKVIPYLVVLVT
ECCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
AVCGINVMLVLFIGIVLSGIVGLWTGSFDIWGWNASMGLGITNMGELIIVTLLAGGMLEM
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
IRYNGGIDWIIRKLTSRIHSSKGAEMSIAALVSFANLCTANNTIALIMAGPIAKDIADRF
HHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHH
KIDPRRSASLLDIFSCFVQGIIPYGAQMLMAAGLGHVSPIEIMQYLYYPYLLGAGALLAI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LFNYPRKYTYV
HHHCCCCCCCC
>Mature Secondary Structure 
GELGRRPNAWALTPLVVFLLTYLVVSIVAGDFYKMPITVAFVISSIVAIAISKGGKLSN
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RIEQFCRGAANSNIMLMVLIFILAGAFAQTAKAMGAVDATVNLAMSILPGNLLAAGIFLA
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ACFISISVGTSVGTIVALAPVAVGIADKTGMPDALMLGVVVSGAMFGDNLSFISDTTIVA
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCEEEE
TRTQGCQMSDKFKVNIRIALPIAILTGLIYVLVGSGVENAYAPAPIEWVKVIPYLVVLVT
ECCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
AVCGINVMLVLFIGIVLSGIVGLWTGSFDIWGWNASMGLGITNMGELIIVTLLAGGMLEM
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
IRYNGGIDWIIRKLTSRIHSSKGAEMSIAALVSFANLCTANNTIALIMAGPIAKDIADRF
HHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHH
KIDPRRSASLLDIFSCFVQGIIPYGAQMLMAAGLGHVSPIEIMQYLYYPYLLGAGALLAI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LFNYPRKYTYV
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]