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The map label for this gene is ald [H]

Identifier: 150007139

GI number: 150007139

Start: 556623

End: 557717

Strand: Reverse

Name: ald [H]

Synonym: BDI_0483

Alternate gene names: 150007139

Gene position: 557717-556623 (Counterclockwise)

Preceding gene: 150007140

Following gene: 150007138

Centisome position: 11.59

GC content: 52.6

Gene sequence:

>1095_bases
ATGATTATTGGAATTCCAAAAGAAATCAAAAACAACGAGAACAGGGTAGCTCTTACTCCCGCCGGAGCCAAAGAGTTGGT
GAAAAGAGGTCATACCGTGTATGTACAACACACGGCTGGTATAAACAGTGGTTTCGCCGATGATGCCTATGTGGCGGCAG
GGGCCCGGATACTCCCGTCCATTGAGGATGTATATGGAATTGCCGAGATGATCGTAAAAGTAAAAGAACCGATCGCCAGC
GAATATCCCTTGGTACGCAAAGGACAGCTTGTCTTCACCTACTTCCATTTCGCATCCGACGAGGCTCTCACCCTTGCCAT
GATAAAGAGCGGCTCCATCTGTCTGGCTTACGAGACCGTGGAGAACCCAGATCATACGTTACCCCTTTTGATTCCTATGA
GTGAGGTGGCCGGACGCATGTCCGTACAGGAAGGCGCTCGTTTCCTTGAGAAACCGCAAGGTGGCAAAGGCGTATTGCTA
GGTGGCGTGCCGGGCGTGAAACCGGGTAAGGTATTGGTTTTAGGCGGTGGTGTGGTAGGCCACAACGCCGCCTTGATGGC
CGCCGGGCTAGGCGCCGATGTCACGATCGCCGACATCTCGCTTCCCCGCTTACGCTATCTGTCCGAGACGATGCCGAAAA
ACGTGAAGACGTTGTTCTCTTCCACGCATACGATCGAACAGGAATTACCCACAACCGATTTAGTGATCGGCGCCGTATTA
ATCCCCGGAGCCAAGGCCCCGCACTTGATCACCCGGGATATGTTGAAATTATTAAAACCGGGCAGCGTATTGGTAGATGT
AGCCATCGATCAAGGCGGTTGCTTCGAGACCTCCCATCCGACTACGCACGCCAATCCGGTTTATGAGATAGACGATATCC
TCCATTACTGCGTAGCCAACATTCCGGGAGCGGTCCCGCAGACTTCCACGTTAGCCTTGACCAACGCTACCCTGCCGTAC
GTGATCAAGCTTGCCGACAGAGGTTGGGAAAAAGCCTGCGAAGAAGATCCGGGCCTAGAACTGGGATTAAACATCGTGGA
AGGAAAGATTGTCTACCCCGCCGTAGCAGAGGCTTTTCCTTCACTCAAAGCATAA

Upstream 100 bases:

>100_bases
ATAAGGAACAAGAAATAAGATAAAAAAAGAGGGACAGAATACCAATCTGTTCCTCTTTTTTGTATATTGCAGGCATATAA
ACCCATTAAATGCTACCATT

Downstream 100 bases:

>100_bases
GTTCCAAAGAATCTTTCATTAGTAGGGATTGCTTGCGGCAATCCCTTTTTTATGTTTTCTATTTTTATTGTTTTCTAAAA
AACGTAAAATATCTTGGCTT

Product: alanine dehydrogenase

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MIIGIPKEIKNNENRVALTPAGAKELVKRGHTVYVQHTAGINSGFADDAYVAAGARILPSIEDVYGIAEMIVKVKEPIAS
EYPLVRKGQLVFTYFHFASDEALTLAMIKSGSICLAYETVENPDHTLPLLIPMSEVAGRMSVQEGARFLEKPQGGKGVLL
GGVPGVKPGKVLVLGGGVVGHNAALMAAGLGADVTIADISLPRLRYLSETMPKNVKTLFSSTHTIEQELPTTDLVIGAVL
IPGAKAPHLITRDMLKLLKPGSVLVDVAIDQGGCFETSHPTTHANPVYEIDDILHYCVANIPGAVPQTSTLALTNATLPY
VIKLADRGWEKACEEDPGLELGLNIVEGKIVYPAVAEAFPSLKA

Sequences:

>Translated_364_residues
MIIGIPKEIKNNENRVALTPAGAKELVKRGHTVYVQHTAGINSGFADDAYVAAGARILPSIEDVYGIAEMIVKVKEPIAS
EYPLVRKGQLVFTYFHFASDEALTLAMIKSGSICLAYETVENPDHTLPLLIPMSEVAGRMSVQEGARFLEKPQGGKGVLL
GGVPGVKPGKVLVLGGGVVGHNAALMAAGLGADVTIADISLPRLRYLSETMPKNVKTLFSSTHTIEQELPTTDLVIGAVL
IPGAKAPHLITRDMLKLLKPGSVLVDVAIDQGGCFETSHPTTHANPVYEIDDILHYCVANIPGAVPQTSTLALTNATLPY
VIKLADRGWEKACEEDPGLELGLNIVEGKIVYPAVAEAFPSLKA
>Mature_364_residues
MIIGIPKEIKNNENRVALTPAGAKELVKRGHTVYVQHTAGINSGFADDAYVAAGARILPSIEDVYGIAEMIVKVKEPIAS
EYPLVRKGQLVFTYFHFASDEALTLAMIKSGSICLAYETVENPDHTLPLLIPMSEVAGRMSVQEGARFLEKPQGGKGVLL
GGVPGVKPGKVLVLGGGVVGHNAALMAAGLGADVTIADISLPRLRYLSETMPKNVKTLFSSTHTIEQELPTTDLVIGAVL
IPGAKAPHLITRDMLKLLKPGSVLVDVAIDQGGCFETSHPTTHANPVYEIDDILHYCVANIPGAVPQTSTLALTNATLPY
VIKLADRGWEKACEEDPGLELGLNIVEGKIVYPAVAEAFPSLKA

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=348, Percent_Identity=31.0344827586207, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI122939153, Length=348, Percent_Identity=31.0344827586207, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1787887, Length=382, Percent_Identity=30.1047120418848, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17550456, Length=372, Percent_Identity=27.6881720430108, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 38653; Mature: 38653

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGIPKEIKNNENRVALTPAGAKELVKRGHTVYVQHTAGINSGFADDAYVAAGARILPS
CEECCCHHHCCCCCEEEECCCCHHHHHHCCCEEEEEEECCCCCCCCCCHHHHCCCHHCCC
IEDVYGIAEMIVKVKEPIASEYPLVRKGQLVFTYFHFASDEALTLAMIKSGSICLAYETV
HHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEEECCCCEEEEEEECCCCEEEEEEEC
ENPDHTLPLLIPMSEVAGRMSVQEGARFLEKPQGGKGVLLGGVPGVKPGKVLVLGGGVVG
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCEEC
HNAALMAAGLGADVTIADISLPRLRYLSETMPKNVKTLFSSTHTIEQELPTTDLVIGAVL
CCCEEEEECCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHEEEHEE
IPGAKAPHLITRDMLKLLKPGSVLVDVAIDQGGCFETSHPTTHANPVYEIDDILHYCVAN
ECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
IPGAVPQTSTLALTNATLPYVIKLADRGWEKACEEDPGLELGLNIVEGKIVYPAVAEAFP
CCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHCCCCCCEEEEEEECCEEECHHHHHHCC
SLKA
CCCC
>Mature Secondary Structure
MIIGIPKEIKNNENRVALTPAGAKELVKRGHTVYVQHTAGINSGFADDAYVAAGARILPS
CEECCCHHHCCCCCEEEECCCCHHHHHHCCCEEEEEEECCCCCCCCCCHHHHCCCHHCCC
IEDVYGIAEMIVKVKEPIASEYPLVRKGQLVFTYFHFASDEALTLAMIKSGSICLAYETV
HHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEEECCCCEEEEEEECCCCEEEEEEEC
ENPDHTLPLLIPMSEVAGRMSVQEGARFLEKPQGGKGVLLGGVPGVKPGKVLVLGGGVVG
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCEEC
HNAALMAAGLGADVTIADISLPRLRYLSETMPKNVKTLFSSTHTIEQELPTTDLVIGAVL
CCCEEEEECCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHEEEHEE
IPGAKAPHLITRDMLKLLKPGSVLVDVAIDQGGCFETSHPTTHANPVYEIDDILHYCVAN
ECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
IPGAVPQTSTLALTNATLPYVIKLADRGWEKACEEDPGLELGLNIVEGKIVYPAVAEAFP
CCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHCCCCCCEEEEEEECCEEECHHHHHHCC
SLKA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]