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The map label for this gene is rlmI [H]

Identifier: 150007049

GI number: 150007049

Start: 448456

End: 449640

Strand: Reverse

Name: rlmI [H]

Synonym: BDI_0392

Alternate gene names: 150007049

Gene position: 449640-448456 (Counterclockwise)

Preceding gene: 150007050

Following gene: 150007046

Centisome position: 9.35

GC content: 44.56

Gene sequence:

>1185_bases
ATGAAGTATTGTAAAGTTTTTCTCAAGCCTAAGAAAGAAGAATCGCTATTGCGTTTCCATCCATGGGTATTTTCCGGAGC
GATCCAATCTGTTGAAGGCGACCCGGAAGAGGGTGACTTGGTAGAGGTATACGGAGCGAACCAGCGTTTTTTGGCGATCG
GCCATTACCAAATCGGCAGTATCGCCGTACGTGTGTTATCTTTCACCCCAATCCCCATCGACGATGCTTTTTGGATCGAA
CGTATCCGTATTGCTTACGAACTCAGAAAGACCTTGCGGCTGGCTGGGGTAGAAAATAATAATACGTTCCGCCTGATTCA
CGGCGAAGGCGATAATTTACCGGGATTGGTGATCGATATGTATGCGCATACGGCGGTTATGCAAGCACATTCGGTAGGAA
TGCATTATGCCCGTCATCAGATCGCAGAGGCTTTGAAAGCGGTATTGGGCGATACTTTACAAAACATCTACTATAAATCT
GAAGCGACTTTACCTTATAAAGCGAATTTAGGTAGCGAAGATGGTTACTTATTCGGAGGCGAAGTGGAAGATATCGCTAT
AGAAAACGGACTGAAATTCTGTGTAGACTGGCAGAAAGGCCAAAAGACAGGTTTCTTCGTGGATCAACGGGAGAATCGCT
CTTTGTTGGAGCATTACGCAAAGGATCGCTCCGTATTGAATATGTTCTGTTATACGGGCGGTTTCTCTTTCTATGCGATG
AGAGGAGGCGCTAAGGTGGTTCATTCCGTAGACAGTTCGGCGAAAGCGATTTCTTTGACGAAAAAGAATGTCGAGTTGAA
TTTCCCGGGAGATCCCCGTCATGAGGCATATGCGGAAGATGCGTTTAAGTATTTGGAAAAAATGGGAAGTAATTATGATT
TAATCATATTGGACCCTCCTGCGTTCGCTAAACATAAGAATGTACTACGTAATGCCTTACAAGGGTATCGCAAATTAAAT
GCGATCGCTTTCGAGAAGATCAAGCCGGGAGGAATCTTGTTTACTTTCTCTTGTTCTCAAGTAGTTAGTAAAGAGAATTT
CCGTTTAGCCGTATTTAGCGCTGCCGCCCAATCCGGCCGTAGTGTCCGAATCTTACATCAATTAACCCAACCCGCAGACC
ATCCGGTGAATATCTACCATCCGGAAGGGGAATATCTGAAAGGTTTAGTATTGTATGTAGAATAA

Upstream 100 bases:

>100_bases
GAATTGGGAGGCAGATGTATTGACAGACTCACAAAAGAAATATGCGGCCCTCGATGCTTGGGCTTGCCTAAGAATATATA
ATTTATTGAATCAAGCATAA

Downstream 100 bases:

>100_bases
GATATGCTCTTTGGGTGAAAACAAAGAATTAGTTTTCACCCAAAATTCAAGTTAATACCCCGGATTTTGTGTCAAGTTCG
GATTCAAGGAACGGACTTTA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKYCKVFLKPKKEESLLRFHPWVFSGAIQSVEGDPEEGDLVEVYGANQRFLAIGHYQIGSIAVRVLSFTPIPIDDAFWIE
RIRIAYELRKTLRLAGVENNNTFRLIHGEGDNLPGLVIDMYAHTAVMQAHSVGMHYARHQIAEALKAVLGDTLQNIYYKS
EATLPYKANLGSEDGYLFGGEVEDIAIENGLKFCVDWQKGQKTGFFVDQRENRSLLEHYAKDRSVLNMFCYTGGFSFYAM
RGGAKVVHSVDSSAKAISLTKKNVELNFPGDPRHEAYAEDAFKYLEKMGSNYDLIILDPPAFAKHKNVLRNALQGYRKLN
AIAFEKIKPGGILFTFSCSQVVSKENFRLAVFSAAAQSGRSVRILHQLTQPADHPVNIYHPEGEYLKGLVLYVE

Sequences:

>Translated_394_residues
MKYCKVFLKPKKEESLLRFHPWVFSGAIQSVEGDPEEGDLVEVYGANQRFLAIGHYQIGSIAVRVLSFTPIPIDDAFWIE
RIRIAYELRKTLRLAGVENNNTFRLIHGEGDNLPGLVIDMYAHTAVMQAHSVGMHYARHQIAEALKAVLGDTLQNIYYKS
EATLPYKANLGSEDGYLFGGEVEDIAIENGLKFCVDWQKGQKTGFFVDQRENRSLLEHYAKDRSVLNMFCYTGGFSFYAM
RGGAKVVHSVDSSAKAISLTKKNVELNFPGDPRHEAYAEDAFKYLEKMGSNYDLIILDPPAFAKHKNVLRNALQGYRKLN
AIAFEKIKPGGILFTFSCSQVVSKENFRLAVFSAAAQSGRSVRILHQLTQPADHPVNIYHPEGEYLKGLVLYVE
>Mature_394_residues
MKYCKVFLKPKKEESLLRFHPWVFSGAIQSVEGDPEEGDLVEVYGANQRFLAIGHYQIGSIAVRVLSFTPIPIDDAFWIE
RIRIAYELRKTLRLAGVENNNTFRLIHGEGDNLPGLVIDMYAHTAVMQAHSVGMHYARHQIAEALKAVLGDTLQNIYYKS
EATLPYKANLGSEDGYLFGGEVEDIAIENGLKFCVDWQKGQKTGFFVDQRENRSLLEHYAKDRSVLNMFCYTGGFSFYAM
RGGAKVVHSVDSSAKAISLTKKNVELNFPGDPRHEAYAEDAFKYLEKMGSNYDLIILDPPAFAKHKNVLRNALQGYRKLN
AIAFEKIKPGGILFTFSCSQVVSKENFRLAVFSAAAQSGRSVRILHQLTQPADHPVNIYHPEGEYLKGLVLYVE

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=397, Percent_Identity=37.5314861460957, Blast_Score=260, Evalue=8e-71,
Organism=Escherichia coli, GI1787181, Length=238, Percent_Identity=27.3109243697479, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44341; Mature: 44341

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYCKVFLKPKKEESLLRFHPWVFSGAIQSVEGDPEEGDLVEVYGANQRFLAIGHYQIGS
CCCEEEEECCCCCCCHHEECCHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEECEEHHH
IAVRVLSFTPIPIDDAFWIERIRIAYELRKTLRLAGVENNNTFRLIHGEGDNLPGLVIDM
HHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEH
YAHTAVMQAHSVGMHYARHQIAEALKAVLGDTLQNIYYKSEATLPYKANLGSEDGYLFGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEECCCCCCCCEEECC
EVEDIAIENGLKFCVDWQKGQKTGFFVDQRENRSLLEHYAKDRSVLNMFCYTGGFSFYAM
CCCEEEECCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHHCHHHHHHHHHHCCCEEEEE
RGGAKVVHSVDSSAKAISLTKKNVELNFPGDPRHEAYAEDAFKYLEKMGSNYDLIILDPP
CCCCCEEECCCCCCEEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCC
AFAKHKNVLRNALQGYRKLNAIAFEKIKPGGILFTFSCSQVVSKENFRLAVFSAAAQSGR
CHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEHHHHHCCCCCEEEEEEEHHHCCC
SVRILHQLTQPADHPVNIYHPEGEYLKGLVLYVE
EEEEHHHHCCCCCCCEEEECCCCCCEEEEEEEEC
>Mature Secondary Structure
MKYCKVFLKPKKEESLLRFHPWVFSGAIQSVEGDPEEGDLVEVYGANQRFLAIGHYQIGS
CCCEEEEECCCCCCCHHEECCHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEECEEHHH
IAVRVLSFTPIPIDDAFWIERIRIAYELRKTLRLAGVENNNTFRLIHGEGDNLPGLVIDM
HHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEH
YAHTAVMQAHSVGMHYARHQIAEALKAVLGDTLQNIYYKSEATLPYKANLGSEDGYLFGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEECCCCCCCCEEECC
EVEDIAIENGLKFCVDWQKGQKTGFFVDQRENRSLLEHYAKDRSVLNMFCYTGGFSFYAM
CCCEEEECCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHHCHHHHHHHHHHCCCEEEEE
RGGAKVVHSVDSSAKAISLTKKNVELNFPGDPRHEAYAEDAFKYLEKMGSNYDLIILDPP
CCCCCEEECCCCCCEEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCC
AFAKHKNVLRNALQGYRKLNAIAFEKIKPGGILFTFSCSQVVSKENFRLAVFSAAAQSGR
CHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEHHHHHCCCCCEEEEEEEHHHCCC
SVRILHQLTQPADHPVNIYHPEGEYLKGLVLYVE
EEEEHHHHCCCCCCCEEEECCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA