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The map label for this gene is mdh [H]

Identifier: 150007046

GI number: 150007046

Start: 442775

End: 443716

Strand: Reverse

Name: mdh [H]

Synonym: BDI_0389

Alternate gene names: 150007046

Gene position: 443716-442775 (Counterclockwise)

Preceding gene: 150007049

Following gene: 150007036

Centisome position: 9.22

GC content: 44.48

Gene sequence:

>942_bases
ATGTCAAAAGTAACAGTTGTTGGTGCCGGAAATGTAGGCGCTACTTGCGCAAATGTTCTTGCCTTCAATGAAGTGGCAGA
CGAAGTTGTAATGCTAGACGTAAAGGAAGGCGTATCCGAAGGTAAAGCGATGGATATGATGCAGACCGCTCAATTGCTAG
GTTTCGATACTAAGGTTGTAGGTTGCACAAACGATTATGAGAAGACAGCTAACTCTGACGTTGTAGTTATCACTTCTGGT
ATTCCTCGTAAACCGGGTATGACTCGCGAAGAACTGATCGGTGTTAACGCTGGTATCGTTAAGAGCGTAGCTCAAAACAT
CTTGAAATACTCTCCGAACGCAATCTTGGTAGTTATCTCTAACCCGATGGATACAATGACTTATTTGTCATTGAAGTCTT
TAGGCTTGCCGAAGAACCGTATCATCGGTATGGGTGGTGCTTTGGATAGCTCTCGTTTCAAATATTTCTTATCTCAGGCT
CTAGGCTGTAACGCTAACGAGGTTGAGGGTATGGTAATCGGTGGTCACGGTGACACTACAATGATTCCGTTGGCTCGCTT
GGCTACTTACAAAGGTATCCCTGTTAGCAAATTGTTAAGCGCAGAGAAATTGCAAGAGGTTGTTGCTTCCACAATGGTTG
GTGGCGCTACATTAACTAAATTGTTAGGTACTTCTGCTTGGTATGCACCGGGTGCTGCTGGAGCATTTGTTGTTGAGTCT
ATCATTCACAACCAAGGTAAGATGGTTCCTTGCTCTGTTTACTTGGAAGGTGAGTACGGTGAATCTGATCTTTGCATCGG
TGTTCCTGTTATCCTTGGCAAGAACGGTATCGAGAAGATCGTTGAGTTGGAATTGACAGCTGAAGAGAAAGAGTTGTTCG
CTAAGAGCGCTGCCGCTGTTCATAAGACTAACGAGGCTTTGAAAGAAGTGGGTGCACTTTAA

Upstream 100 bases:

>100_bases
TATACGGAAATATTTCTTTATTCCGTAATAGATTGTACCTTTGCCATCGTTAAAAAACATAGCGTATTATTAAGTAATAA
CATACTAAAACTTATTTATT

Downstream 100 bases:

>100_bases
TTTGAATTTCTATTAAAGCGATAAAAGAGGCTGATCCTTCGGGGTTGGCCTCTTTCTTTTTATGTGGATACGGATAGTTA
CGACGGAATGAAGATAATAT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSKVTVVGAGNVGATCANVLAFNEVADEVVMLDVKEGVSEGKAMDMMQTAQLLGFDTKVVGCTNDYEKTANSDVVVITSG
IPRKPGMTREELIGVNAGIVKSVAQNILKYSPNAILVVISNPMDTMTYLSLKSLGLPKNRIIGMGGALDSSRFKYFLSQA
LGCNANEVEGMVIGGHGDTTMIPLARLATYKGIPVSKLLSAEKLQEVVASTMVGGATLTKLLGTSAWYAPGAAGAFVVES
IIHNQGKMVPCSVYLEGEYGESDLCIGVPVILGKNGIEKIVELELTAEEKELFAKSAAAVHKTNEALKEVGAL

Sequences:

>Translated_313_residues
MSKVTVVGAGNVGATCANVLAFNEVADEVVMLDVKEGVSEGKAMDMMQTAQLLGFDTKVVGCTNDYEKTANSDVVVITSG
IPRKPGMTREELIGVNAGIVKSVAQNILKYSPNAILVVISNPMDTMTYLSLKSLGLPKNRIIGMGGALDSSRFKYFLSQA
LGCNANEVEGMVIGGHGDTTMIPLARLATYKGIPVSKLLSAEKLQEVVASTMVGGATLTKLLGTSAWYAPGAAGAFVVES
IIHNQGKMVPCSVYLEGEYGESDLCIGVPVILGKNGIEKIVELELTAEEKELFAKSAAAVHKTNEALKEVGAL
>Mature_312_residues
SKVTVVGAGNVGATCANVLAFNEVADEVVMLDVKEGVSEGKAMDMMQTAQLLGFDTKVVGCTNDYEKTANSDVVVITSGI
PRKPGMTREELIGVNAGIVKSVAQNILKYSPNAILVVISNPMDTMTYLSLKSLGLPKNRIIGMGGALDSSRFKYFLSQAL
GCNANEVEGMVIGGHGDTTMIPLARLATYKGIPVSKLLSAEKLQEVVASTMVGGATLTKLLGTSAWYAPGAAGAFVVESI
IHNQGKMVPCSVYLEGEYGESDLCIGVPVILGKNGIEKIVELELTAEEKELFAKSAAAVHKTNEALKEVGAL

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=315, Percent_Identity=34.9206349206349, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI221136809, Length=315, Percent_Identity=34.9206349206349, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI5031857, Length=315, Percent_Identity=36.1904761904762, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI260099723, Length=315, Percent_Identity=36.1904761904762, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI15082234, Length=315, Percent_Identity=33.3333333333333, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI9257228, Length=313, Percent_Identity=33.5463258785943, Blast_Score=184, Evalue=9e-47,
Organism=Homo sapiens, GI4504973, Length=313, Percent_Identity=33.5463258785943, Blast_Score=184, Evalue=9e-47,
Organism=Homo sapiens, GI291575128, Length=322, Percent_Identity=32.2981366459627, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI4557032, Length=322, Percent_Identity=32.2981366459627, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI260099727, Length=180, Percent_Identity=41.1111111111111, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI260099725, Length=180, Percent_Identity=41.1111111111111, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI103472011, Length=315, Percent_Identity=27.9365079365079, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI207028494, Length=199, Percent_Identity=33.6683417085427, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI21735621, Length=293, Percent_Identity=30.7167235494881, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI103472015, Length=182, Percent_Identity=30.2197802197802, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1789632, Length=291, Percent_Identity=31.9587628865979, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17535107, Length=294, Percent_Identity=30.2721088435374, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17554310, Length=319, Percent_Identity=32.2884012539185, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322765, Length=304, Percent_Identity=30.9210526315789, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320125, Length=201, Percent_Identity=31.8407960199005, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6324446, Length=166, Percent_Identity=31.3253012048193, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI17136226, Length=314, Percent_Identity=32.8025477707006, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI45550422, Length=307, Percent_Identity=28.9902280130293, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24647881, Length=310, Percent_Identity=32.5806451612903, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24663599, Length=308, Percent_Identity=30.5194805194805, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24663595, Length=282, Percent_Identity=29.0780141843972, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 32849; Mature: 32717

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVTVVGAGNVGATCANVLAFNEVADEVVMLDVKEGVSEGKAMDMMQTAQLLGFDTKVV
CCEEEEEECCCCCHHHHHHHHHHHHHHHEEEEEHHHCCCCCHHHHHHHHHHHHCCCCEEE
GCTNDYEKTANSDVVVITSGIPRKPGMTREELIGVNAGIVKSVAQNILKYSPNAILVVIS
ECCCCHHHCCCCCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEC
NPMDTMTYLSLKSLGLPKNRIIGMGGALDSSRFKYFLSQALGCNANEVEGMVIGGHGDTT
CCCHHHHHHHHHHCCCCHHHEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCH
MIPLARLATYKGIPVSKLLSAEKLQEVVASTMVGGATLTKLLGTSAWYAPGAAGAFVVES
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHH
IIHNQGKMVPCSVYLEGEYGESDLCIGVPVILGKNGIEKIVELELTAEEKELFAKSAAAV
HHHCCCCEEEEEEEEECCCCCCCEEEECHHEECCCHHHHHHEEEECHHHHHHHHHHHHHH
HKTNEALKEVGAL
HHHHHHHHHHCCC
>Mature Secondary Structure 
SKVTVVGAGNVGATCANVLAFNEVADEVVMLDVKEGVSEGKAMDMMQTAQLLGFDTKVV
CEEEEEECCCCCHHHHHHHHHHHHHHHEEEEEHHHCCCCCHHHHHHHHHHHHCCCCEEE
GCTNDYEKTANSDVVVITSGIPRKPGMTREELIGVNAGIVKSVAQNILKYSPNAILVVIS
ECCCCHHHCCCCCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEC
NPMDTMTYLSLKSLGLPKNRIIGMGGALDSSRFKYFLSQALGCNANEVEGMVIGGHGDTT
CCCHHHHHHHHHHCCCCHHHEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCH
MIPLARLATYKGIPVSKLLSAEKLQEVVASTMVGGATLTKLLGTSAWYAPGAAGAFVVES
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHH
IIHNQGKMVPCSVYLEGEYGESDLCIGVPVILGKNGIEKIVELELTAEEKELFAKSAAAV
HHHCCCCEEEEEEEEECCCCCCCEEEECHHEECCCHHHHHHEEEECHHHHHHHHHHHHHH
HKTNEALKEVGAL
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA