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The map label for this gene is 150007013

Identifier: 150007013

GI number: 150007013

Start: 400995

End: 402044

Strand: Reverse

Name: 150007013

Synonym: BDI_0350

Alternate gene names: NA

Gene position: 402044-400995 (Counterclockwise)

Preceding gene: 150007018

Following gene: 150007005

Centisome position: 8.36

GC content: 51.43

Gene sequence:

>1050_bases
ATGAAATCAAAGAATTTAACACTGGCCTCTCTTTTCGCCGGCTTGTTCATGTTTATGAACCCTATGGGAAGCGAGGCGTG
TACAAGGGCCGTTTACCTCGGGCCGGACGATATGGTCGTAACCGGACGTACCATGGATTGGAAAGAGGACCCGCAATCCA
ACCTGTATCTCTTCCCCCGGGGCGTACAGAGAAGGGGAGCGATATCCGATAATACCATCCAATGGACCTCGAAATACGGA
AGCGTGGTGACCGCCGGGTATGATATCGGTACGTGCGACGGCATGAACGAGAAGGGACTTGTTGCCAACCTATTGTTCCT
CACCGAGTCATCTTATTTCCGACCGGACGATAACCGCCCGGTTATGGGGCTTAGCATCTGGACGCAGTATGTACTCGATA
ATTTCGCCACGGTAGATGAGGCGGTTGCCGAGTTAAGCAAGGAGGTCTTCCGCATCGACGATCCGGATCTTCCGAACGGG
GCGAAATCAACCTTGCATCTCTCGATCTCGGACGCATCAGGGAATAGCGCTATCTTCGAGTACCTAAATGGGAACTTGGT
TATCCATGAGGGACGGGAATGCCAAGTCATGACGAATTCCCCTACATACGATAAGCAACTAACCTTAAACGATTACTGGC
AACAGATAGGCGGCTTGGTCATGTTGCCGGGTACGAACCGGGCTTCCGATCGTTTCGTGCGTGCCTCTTTCTATATCCAC
GCCATCCCGCAGACAAGTAATTTCCGGGAAGCGGTAGCCGGGGTATTCAGCGTAATGCGTAACGTATCGGTACCTCTGGG
AATCACGACCCCGGATCAACCGAATATCTCCTCTACCCGCTGGCGTACGGTAGCCGACCAGAAGAATAAGGTATATTACT
TCGAGTCTACTTTAAGCCCGGATATCTTTTGGGTCGATTTCAAGAGCTTGGACTTCAAGGCAGGTACACCTATCAAGAAA
CTAACGCTCACAAACGGCGAGATTTACGCCGGAAACACCGCCAAAGATTTCAAGGACAGCAAGCCGTTACCGTTCTTGTT
CGGGATCTGA

Upstream 100 bases:

>100_bases
ATGGGGGTTGTTCACACGGCTCCGTATCCCGTATATCATTCTAAAACATTCCTTAAAGCCACTTGTTTCATAAATAGATT
ATCCTAATAATATAGATCTT

Downstream 100 bases:

>100_bases
AGGACTAGAAAAACAGACAAGAATAAATAAGGGGAGGTATCAAAAGTCGGTATTTACTCCCCCCTTATTTTTAAACAAGC
GTTATTACCTTTTATGATAT

Product: choloylglycine hydrolase

Products: NA

Alternate protein names: Penicillin V amidase; PVA [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKSKNLTLASLFAGLFMFMNPMGSEACTRAVYLGPDDMVVTGRTMDWKEDPQSNLYLFPRGVQRRGAISDNTIQWTSKYG
SVVTAGYDIGTCDGMNEKGLVANLLFLTESSYFRPDDNRPVMGLSIWTQYVLDNFATVDEAVAELSKEVFRIDDPDLPNG
AKSTLHLSISDASGNSAIFEYLNGNLVIHEGRECQVMTNSPTYDKQLTLNDYWQQIGGLVMLPGTNRASDRFVRASFYIH
AIPQTSNFREAVAGVFSVMRNVSVPLGITTPDQPNISSTRWRTVADQKNKVYYFESTLSPDIFWVDFKSLDFKAGTPIKK
LTLTNGEIYAGNTAKDFKDSKPLPFLFGI

Sequences:

>Translated_349_residues
MKSKNLTLASLFAGLFMFMNPMGSEACTRAVYLGPDDMVVTGRTMDWKEDPQSNLYLFPRGVQRRGAISDNTIQWTSKYG
SVVTAGYDIGTCDGMNEKGLVANLLFLTESSYFRPDDNRPVMGLSIWTQYVLDNFATVDEAVAELSKEVFRIDDPDLPNG
AKSTLHLSISDASGNSAIFEYLNGNLVIHEGRECQVMTNSPTYDKQLTLNDYWQQIGGLVMLPGTNRASDRFVRASFYIH
AIPQTSNFREAVAGVFSVMRNVSVPLGITTPDQPNISSTRWRTVADQKNKVYYFESTLSPDIFWVDFKSLDFKAGTPIKK
LTLTNGEIYAGNTAKDFKDSKPLPFLFGI
>Mature_349_residues
MKSKNLTLASLFAGLFMFMNPMGSEACTRAVYLGPDDMVVTGRTMDWKEDPQSNLYLFPRGVQRRGAISDNTIQWTSKYG
SVVTAGYDIGTCDGMNEKGLVANLLFLTESSYFRPDDNRPVMGLSIWTQYVLDNFATVDEAVAELSKEVFRIDDPDLPNG
AKSTLHLSISDASGNSAIFEYLNGNLVIHEGRECQVMTNSPTYDKQLTLNDYWQQIGGLVMLPGTNRASDRFVRASFYIH
AIPQTSNFREAVAGVFSVMRNVSVPLGITTPDQPNISSTRWRTVADQKNKVYYFESTLSPDIFWVDFKSLDFKAGTPIKK
LTLTNGEIYAGNTAKDFKDSKPLPFLFGI

Specific function: The enzyme catalyzes the conversion of penicillin to 6- aminopenicillanate The precursor, furthermore, acts as a self- processing peptidase that cleaves off the propeptide. All peptidase activity is lost on conversion to the mature peptidase [H]

COG id: COG3049

COG function: function code M; Penicillin V acylase and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C59 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003199 [H]

Pfam domain/function: PF02275 CBAH [H]

EC number: =3.5.1.11 [H]

Molecular weight: Translated: 38906; Mature: 38906

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKNLTLASLFAGLFMFMNPMGSEACTRAVYLGPDDMVVTGRTMDWKEDPQSNLYLFPR
CCCCCCHHHHHHHHHHHHHCCCCCHHHCEEEEECCCCEEEECCCCCCCCCCCCCEEEEEC
GVQRRGAISDNTIQWTSKYGSVVTAGYDIGTCDGMNEKGLVANLLFLTESSYFRPDDNRP
CCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCC
VMGLSIWTQYVLDNFATVDEAVAELSKEVFRIDDPDLPNGAKSTLHLSISDASGNSAIFE
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEEEECCCCCHHHHE
YLNGNLVIHEGRECQVMTNSPTYDKQLTLNDYWQQIGGLVMLPGTNRASDRFVRASFYIH
ECCCCEEEECCCEEEEEECCCCCCCEEEHHHHHHHHCCEEEECCCCCCCCCEEEEEEEEE
AIPQTSNFREAVAGVFSVMRNVSVPLGITTPDQPNISSTRWRTVADQKNKVYYFESTLSP
ECCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEECCCCCEEEEEECCCCC
DIFWVDFKSLDFKAGTPIKKLTLTNGEIYAGNTAKDFKDSKPLPFLFGI
CEEEEEECCCCCCCCCCEEEEEEECCEEEECCCHHHCCCCCCCCEECCC
>Mature Secondary Structure
MKSKNLTLASLFAGLFMFMNPMGSEACTRAVYLGPDDMVVTGRTMDWKEDPQSNLYLFPR
CCCCCCHHHHHHHHHHHHHCCCCCHHHCEEEEECCCCEEEECCCCCCCCCCCCCEEEEEC
GVQRRGAISDNTIQWTSKYGSVVTAGYDIGTCDGMNEKGLVANLLFLTESSYFRPDDNRP
CCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCC
VMGLSIWTQYVLDNFATVDEAVAELSKEVFRIDDPDLPNGAKSTLHLSISDASGNSAIFE
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEEEECCCCCHHHHE
YLNGNLVIHEGRECQVMTNSPTYDKQLTLNDYWQQIGGLVMLPGTNRASDRFVRASFYIH
ECCCCEEEECCCEEEEEECCCCCCCEEEHHHHHHHHCCEEEECCCCCCCCCEEEEEEEEE
AIPQTSNFREAVAGVFSVMRNVSVPLGITTPDQPNISSTRWRTVADQKNKVYYFESTLSP
ECCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEECCCCCEEEEEECCCCC
DIFWVDFKSLDFKAGTPIKKLTLTNGEIYAGNTAKDFKDSKPLPFLFGI
CEEEEEECCCCCCCCCCEEEEEEECCEEEECCCHHHCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3026906 [H]