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The map label for this gene is nadE [H]

Identifier: 150006982

GI number: 150006982

Start: 355612

End: 357534

Strand: Reverse

Name: nadE [H]

Synonym: BDI_0318

Alternate gene names: 150006982

Gene position: 357534-355612 (Counterclockwise)

Preceding gene: 150006984

Following gene: 150006977

Centisome position: 7.43

GC content: 53.98

Gene sequence:

>1923_bases
ATGAATTACGGATTCGTCAAAGTAGCGGCAGCCGTACCACATGTTAAAGTGGCTGACTGCTTTTATAATATTCAACAGAT
CGAGGGATTGATGCGCCAAGCGTCCCAAAAAGAGGTCCAGATCATCGCTTTCCCCGAATTATCGGTTACGTCTTACACTT
GCATGGACTTGTTCTCGCAAGAGACCTTACTCAGAAACGCCGAGAAAGCCTTGCTGGATTTAGTGAACAACACCGCCGAT
CTGGATTTACTAACGATCGTAGGTTGCCCGCTTGTCAGCGGAAGCCAACTGATAAACGCCGCCGTCGCTTTCCAACGGGG
CGAGATACTAGGGGTAGTGCCCAAGAGCTACCTGCCCAGCTACAAGGAGTTCCAAGAAGAGCGTTGGTTCACGGCCTCTT
CACACCTACAGCAATCCATGATCACGATCGGTAACCGGGAAGTGCCGTTGGATTGCTACCTGATCTTCGAGTATGATGAG
GTACGCGTGGGTATCGAGATCTGCGAGGATCTTTGGGTTCCCATCCCTCCCAGCTCCGAGCTAGCCATGCAGGGGGCGAA
CTTGATATTCAATCTATCCGCCAGCAACGAGTTGATCGGTAAGCATGCTTACTTACGATCCTTGATCTGCCAGCAATCCG
CACGTTGTATCGCCGGATACGTCTACGCATCCGCCGGTTTCGGGGAATCCAGTACCGACCTCGTATTCGCGGGAAACGGT
ATCATAGCGGAGAACGGCACGTTGTTAAGGGAATCCGAGCGTTTCTCCATGGAGGAGCAACTCGTTATCAGCGAGATCGA
TATCCAGAACTTACAGAACGACCGACGGATCAACACCAGTTTCATGCAGGGTTATCTCAACCATAACATGGATAACGGCA
CCGTAGTCTCATTCAGCCTTCCCAGCCGGGCGCTGGACTTGACCCGGGTAATCGACCCGCATCCCTTTACCCCTTCCGGC
GATGCCTTGAAAGAACGTTGCGAGGAAATATTCCATATACAGGTAGCGGGACTCGCCAAGCGTGTCCTCCATGCCCACGC
CCAAACCGCCGTCGTAGGCATCTCTGGGGGACTGGATTCCACCTTGGCCCTACTGGTCACCGTCATGACCTTCGATGCCT
TGAAGATCCCACGGGATAAGATCATCGGCATCACCATGCCCGGCTTCGGGACGACGGATCGTACCTATACCAATGCCTGC
GACTTGATACGCTCCCTGGGCGTGACCCTCCGGGAGATCTCCATCAAGGAGGCTTGCGTCCAACATTTCAAGGATATTGA
TCACGACATCCATGTACATGACGTGACCTACGAGAACAGCCAAGCCCGCGAGCGTACCCAACTTCTTATGGACGTGGCGA
ACCAAGCGAACGGCCTCGTGATCGGTACGGGGGATCTCTCGGAGCTTGCCTTGGGCTGGGCTACTTACAATGGCGACCAT
ATGTCTATGTACGGCGTCAACGGTAGCATTCCCAAGACATTGGTCAAATATCTGGTGGAATGGGTTGCCAACAACAAGGT
AGACGAGGCTTCCCGCACCACGTTATTAGATATAGTAGACACTCCGATCAGCCCGGAATTGATTCCCGCCGACGAGCATG
GAAATATCAAGCAGAAGACGGAGGATTTGGTAGGCCCCTACGAGCTGCACGATTTCTTCCTCTATCACTTCCTGCGCTTC
GGTGCCTCCCCCGCGAAGATCTACTTCTTGGCGAAGCGGGCATTCGGGGAAACCTACGACGATGCCACCCTCAAGAAATG
GCTCCATACCTTTTTCCGCCGCTTCTTCCAGCAACAGTTCAAGCGCAGTTGCCTGCCCGACGGCCCGAAAGTAGGCTCCA
TCAGCCTCTCCCCGCGTGGAGACTGGCGAATGCCGAGCGACGCGATGGCTACGCTTTGGCTGGATGAGATCGCAGGGCTT
TAA

Upstream 100 bases:

>100_bases
CTATTTTATAGAAGATGATATCATCTATATCTCCGCAATATTCGACTGTCGTCAAAACCCCGATAAATTAAGAACATCTA
TCAGATAAACAATAACAAAT

Downstream 100 bases:

>100_bases
CATTCGTTCACGGGTATGGTCATAGACGAGGCTCTCTCATGGGTGTCGAAGCCTCGGCTATGACCCGTGAGAGAGCCTCA
GTTATGATCGGTGAGAAAGC

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 640; Mature: 640

Protein sequence:

>640_residues
MNYGFVKVAAAVPHVKVADCFYNIQQIEGLMRQASQKEVQIIAFPELSVTSYTCMDLFSQETLLRNAEKALLDLVNNTAD
LDLLTIVGCPLVSGSQLINAAVAFQRGEILGVVPKSYLPSYKEFQEERWFTASSHLQQSMITIGNREVPLDCYLIFEYDE
VRVGIEICEDLWVPIPPSSELAMQGANLIFNLSASNELIGKHAYLRSLICQQSARCIAGYVYASAGFGESSTDLVFAGNG
IIAENGTLLRESERFSMEEQLVISEIDIQNLQNDRRINTSFMQGYLNHNMDNGTVVSFSLPSRALDLTRVIDPHPFTPSG
DALKERCEEIFHIQVAGLAKRVLHAHAQTAVVGISGGLDSTLALLVTVMTFDALKIPRDKIIGITMPGFGTTDRTYTNAC
DLIRSLGVTLREISIKEACVQHFKDIDHDIHVHDVTYENSQARERTQLLMDVANQANGLVIGTGDLSELALGWATYNGDH
MSMYGVNGSIPKTLVKYLVEWVANNKVDEASRTTLLDIVDTPISPELIPADEHGNIKQKTEDLVGPYELHDFFLYHFLRF
GASPAKIYFLAKRAFGETYDDATLKKWLHTFFRRFFQQQFKRSCLPDGPKVGSISLSPRGDWRMPSDAMATLWLDEIAGL

Sequences:

>Translated_640_residues
MNYGFVKVAAAVPHVKVADCFYNIQQIEGLMRQASQKEVQIIAFPELSVTSYTCMDLFSQETLLRNAEKALLDLVNNTAD
LDLLTIVGCPLVSGSQLINAAVAFQRGEILGVVPKSYLPSYKEFQEERWFTASSHLQQSMITIGNREVPLDCYLIFEYDE
VRVGIEICEDLWVPIPPSSELAMQGANLIFNLSASNELIGKHAYLRSLICQQSARCIAGYVYASAGFGESSTDLVFAGNG
IIAENGTLLRESERFSMEEQLVISEIDIQNLQNDRRINTSFMQGYLNHNMDNGTVVSFSLPSRALDLTRVIDPHPFTPSG
DALKERCEEIFHIQVAGLAKRVLHAHAQTAVVGISGGLDSTLALLVTVMTFDALKIPRDKIIGITMPGFGTTDRTYTNAC
DLIRSLGVTLREISIKEACVQHFKDIDHDIHVHDVTYENSQARERTQLLMDVANQANGLVIGTGDLSELALGWATYNGDH
MSMYGVNGSIPKTLVKYLVEWVANNKVDEASRTTLLDIVDTPISPELIPADEHGNIKQKTEDLVGPYELHDFFLYHFLRF
GASPAKIYFLAKRAFGETYDDATLKKWLHTFFRRFFQQQFKRSCLPDGPKVGSISLSPRGDWRMPSDAMATLWLDEIAGL
>Mature_640_residues
MNYGFVKVAAAVPHVKVADCFYNIQQIEGLMRQASQKEVQIIAFPELSVTSYTCMDLFSQETLLRNAEKALLDLVNNTAD
LDLLTIVGCPLVSGSQLINAAVAFQRGEILGVVPKSYLPSYKEFQEERWFTASSHLQQSMITIGNREVPLDCYLIFEYDE
VRVGIEICEDLWVPIPPSSELAMQGANLIFNLSASNELIGKHAYLRSLICQQSARCIAGYVYASAGFGESSTDLVFAGNG
IIAENGTLLRESERFSMEEQLVISEIDIQNLQNDRRINTSFMQGYLNHNMDNGTVVSFSLPSRALDLTRVIDPHPFTPSG
DALKERCEEIFHIQVAGLAKRVLHAHAQTAVVGISGGLDSTLALLVTVMTFDALKIPRDKIIGITMPGFGTTDRTYTNAC
DLIRSLGVTLREISIKEACVQHFKDIDHDIHVHDVTYENSQARERTQLLMDVANQANGLVIGTGDLSELALGWATYNGDH
MSMYGVNGSIPKTLVKYLVEWVANNKVDEASRTTLLDIVDTPISPELIPADEHGNIKQKTEDLVGPYELHDFFLYHFLRF
GASPAKIYFLAKRAFGETYDDATLKKWLHTFFRRFFQQQFKRSCLPDGPKVGSISLSPRGDWRMPSDAMATLWLDEIAGL

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=263, Percent_Identity=24.7148288973384, Blast_Score=93, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17538756, Length=268, Percent_Identity=29.4776119402985, Blast_Score=105, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321865, Length=588, Percent_Identity=22.4489795918367, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24641841, Length=562, Percent_Identity=22.7758007117438, Blast_Score=97, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24641843, Length=562, Percent_Identity=22.7758007117438, Blast_Score=97, Evalue=5e-20,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 71288; Mature: 71288

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYGFVKVAAAVPHVKVADCFYNIQQIEGLMRQASQKEVQIIAFPELSVTSYTCMDLFSQ
CCCCEEEEEHHCCCEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHH
ETLLRNAEKALLDLVNNTADLDLLTIVGCPLVSGSQLINAAVAFQRGEILGVVPKSYLPS
HHHHHHHHHHHHHHHCCCCCCEEHEECCCCCCCCHHHHHHHHHHCCCCEEEECCHHHCCC
YKEFQEERWFTASSHLQQSMITIGNREVPLDCYLIFEYDEVRVGIEICEDLWVPIPPSSE
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCHHHHHHHHHHCCCCCCCCHH
LAMQGANLIFNLSASNELIGKHAYLRSLICQQSARCIAGYVYASAGFGESSTDLVFAGNG
HHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEECCE
IIAENGTLLRESERFSMEEQLVISEIDIQNLQNDRRINTSFMQGYLNHNMDNGTVVSFSL
EEECCCCEEECHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEEEC
PSRALDLTRVIDPHPFTPSGDALKERCEEIFHIQVAGLAKRVLHAHAQTAVVGISGGLDS
CCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHH
TLALLVTVMTFDALKIPRDKIIGITMPGFGTTDRTYTNACDLIRSLGVTLREISIKEACV
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
QHFKDIDHDIHVHDVTYENSQARERTQLLMDVANQANGLVIGTGDLSELALGWATYNGDH
HHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHEEECCCE
MSMYGVNGSIPKTLVKYLVEWVANNKVDEASRTTLLDIVDTPISPELIPADEHGNIKQKT
EEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHEEEEECCCCCCCCEECCCCCCCCHHHH
EDLVGPYELHDFFLYHFLRFGASPAKIYFLAKRAFGETYDDATLKKWLHTFFRRFFQQQF
HHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
KRSCLPDGPKVGSISLSPRGDWRMPSDAMATLWLDEIAGL
HHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNYGFVKVAAAVPHVKVADCFYNIQQIEGLMRQASQKEVQIIAFPELSVTSYTCMDLFSQ
CCCCEEEEEHHCCCEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHH
ETLLRNAEKALLDLVNNTADLDLLTIVGCPLVSGSQLINAAVAFQRGEILGVVPKSYLPS
HHHHHHHHHHHHHHHCCCCCCEEHEECCCCCCCCHHHHHHHHHHCCCCEEEECCHHHCCC
YKEFQEERWFTASSHLQQSMITIGNREVPLDCYLIFEYDEVRVGIEICEDLWVPIPPSSE
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCHHHHHHHHHHCCCCCCCCHH
LAMQGANLIFNLSASNELIGKHAYLRSLICQQSARCIAGYVYASAGFGESSTDLVFAGNG
HHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEECCE
IIAENGTLLRESERFSMEEQLVISEIDIQNLQNDRRINTSFMQGYLNHNMDNGTVVSFSL
EEECCCCEEECHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEEEC
PSRALDLTRVIDPHPFTPSGDALKERCEEIFHIQVAGLAKRVLHAHAQTAVVGISGGLDS
CCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHH
TLALLVTVMTFDALKIPRDKIIGITMPGFGTTDRTYTNACDLIRSLGVTLREISIKEACV
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
QHFKDIDHDIHVHDVTYENSQARERTQLLMDVANQANGLVIGTGDLSELALGWATYNGDH
HHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHEEECCCE
MSMYGVNGSIPKTLVKYLVEWVANNKVDEASRTTLLDIVDTPISPELIPADEHGNIKQKT
EEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHEEEEECCCCCCCCEECCCCCCCCHHHH
EDLVGPYELHDFFLYHFLRFGASPAKIYFLAKRAFGETYDDATLKKWLHTFFRRFFQQQF
HHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
KRSCLPDGPKVGSISLSPRGDWRMPSDAMATLWLDEIAGL
HHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]