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The map label for this gene is miaA

Identifier: 150006950

GI number: 150006950

Start: 322107

End: 323030

Strand: Reverse

Name: miaA

Synonym: BDI_0286

Alternate gene names: 150006950

Gene position: 323030-322107 (Counterclockwise)

Preceding gene: 150006951

Following gene: 150006949

Centisome position: 6.71

GC content: 46.54

Gene sequence:

>924_bases
ATGAAATCATATGAACTTATCACGATTCTAGGGCCTACGGCTTCTGGAAAGACGACCTTCGCCGCCGCTCTCGCCGCCCA
GTTGGATACGGAGATCATCAGCGCTGATTCCCGGCAGATCTATCGTTCTATGGATATCGGGACGGGTAAGGATTTGGCCG
ATTACAACGTGAATGGAAAACAAATCCCCTATCACTTGATCGATATCTGCGAGCCTGGCTATAAATATAACGTATTTGAG
TATCAGCATGACTTTTTCCGGGTCTATGAGGATATGAAGCGGAGGGGTAAACTCCCGATCTTATGTGGAGGAACCGGGAT
GTACATAGAGGCCGTACTAAAAGGATATAAGCTATTAGACGTGCCTCAAAATCCTGAGCTAAGGGAATCCTTGAGGAATA
AAACCTTGGAGGAACTGGAAACCATCCTCGCCTCTTATAAGATATTACATAATAAAACGGACGTGGACACCGCCCAGCGG
GCTATCCGTGCGATCGAGATCGAGGAGTATTATAAGACTCAGGCACCAGACGTAAATGAATATAATCCGATCAACAGCCT
GATCATCGGTATCCACATCGACCGGGAATTACGTAGGGAAAAAATATCCAGACGCTTGCGGACCCGGTTGGACGAAGGAA
TGGTGGATGAGGTTCGTACGATCTTGGCTACGGGTGTTAAACCGGAAGACTTGATCTATTACGGATTGGAATATAAGTTT
CTTACTTTATATATTATCGGGGAGCTTTCTTTTGAGGAGATGGTCTCGCAACTCGAGATAGCGATCCATCAATTCGCCAA
GAGGCAAATGACTTGGTTCCGGGGCATGGAGCGCCGGGGTTGCGAGATTCATTGGATAGACGCCACTCTACCGACCGAAG
AAAAAATAGCGACAACAATGCGAATTCTAAATAATCAGCTATGA

Upstream 100 bases:

>100_bases
TGAGATGGCTCGCGTTATCGCTGGTAAGTAATTTATTACCACTCTACATAGAAAAGGCTATCCTCTTTGAGGATGGCCTT
TTTTTTCGTACGTTTGTATC

Downstream 100 bases:

>100_bases
TAACAATAAACGATCTAAATAAAAGCGAGAACTTCTTCTTGATGGCAGGTCCTTGCGTAATCGAGGGGGAAGATATGGCG
TTACGTATCGCCGAGAAAAT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase 1; DMAPP:tRNA dimethylallyltransferase 1; DMATase 1; Isopentenyl-diphosphate:tRNA isopentenyltransferase 1; IPP transferase 1; IPPT 1; IPTase 1

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKSYELITILGPTASGKTTFAAALAAQLDTEIISADSRQIYRSMDIGTGKDLADYNVNGKQIPYHLIDICEPGYKYNVFE
YQHDFFRVYEDMKRRGKLPILCGGTGMYIEAVLKGYKLLDVPQNPELRESLRNKTLEELETILASYKILHNKTDVDTAQR
AIRAIEIEEYYKTQAPDVNEYNPINSLIIGIHIDRELRREKISRRLRTRLDEGMVDEVRTILATGVKPEDLIYYGLEYKF
LTLYIIGELSFEEMVSQLEIAIHQFAKRQMTWFRGMERRGCEIHWIDATLPTEEKIATTMRILNNQL

Sequences:

>Translated_307_residues
MKSYELITILGPTASGKTTFAAALAAQLDTEIISADSRQIYRSMDIGTGKDLADYNVNGKQIPYHLIDICEPGYKYNVFE
YQHDFFRVYEDMKRRGKLPILCGGTGMYIEAVLKGYKLLDVPQNPELRESLRNKTLEELETILASYKILHNKTDVDTAQR
AIRAIEIEEYYKTQAPDVNEYNPINSLIIGIHIDRELRREKISRRLRTRLDEGMVDEVRTILATGVKPEDLIYYGLEYKF
LTLYIIGELSFEEMVSQLEIAIHQFAKRQMTWFRGMERRGCEIHWIDATLPTEEKIATTMRILNNQL
>Mature_307_residues
MKSYELITILGPTASGKTTFAAALAAQLDTEIISADSRQIYRSMDIGTGKDLADYNVNGKQIPYHLIDICEPGYKYNVFE
YQHDFFRVYEDMKRRGKLPILCGGTGMYIEAVLKGYKLLDVPQNPELRESLRNKTLEELETILASYKILHNKTDVDTAQR
AIRAIEIEEYYKTQAPDVNEYNPINSLIIGIHIDRELRREKISRRLRTRLDEGMVDEVRTILATGVKPEDLIYYGLEYKF
LTLYIIGELSFEEMVSQLEIAIHQFAKRQMTWFRGMERRGCEIHWIDATLPTEEKIATTMRILNNQL

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=240, Percent_Identity=27.5, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1790613, Length=309, Percent_Identity=32.3624595469256, Blast_Score=135, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI25144712, Length=115, Percent_Identity=31.304347826087, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6324848, Length=319, Percent_Identity=24.4514106583072, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24649830, Length=177, Percent_Identity=29.3785310734463, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA1_PARD8 (A6L8Q7)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001301693.1
- ProteinModelPortal:   A6L8Q7
- SMR:   A6L8Q7
- STRING:   A6L8Q7
- GeneID:   5305441
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_0286
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   IYYGLEY
- ProtClustDB:   PRK00091
- BioCyc:   PDIS435591:BDI_0286-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35576; Mature: 35576

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSYELITILGPTASGKTTFAAALAAQLDTEIISADSRQIYRSMDIGTGKDLADYNVNGK
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCC
QIPYHLIDICEPGYKYNVFEYQHDFFRVYEDMKRRGKLPILCGGTGMYIEAVLKGYKLLD
CCCHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHCCCEEEE
VPQNPELRESLRNKTLEELETILASYKILHNKTDVDTAQRAIRAIEIEEYYKTQAPDVNE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
YNPINSLIIGIHIDRELRREKISRRLRTRLDEGMVDEVRTILATGVKPEDLIYYGLEYKF
CCCHHHEEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHEEEECCCEEE
LTLYIIGELSFEEMVSQLEIAIHQFAKRQMTWFRGMERRGCEIHWIDATLPTEEKIATTM
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHH
RILNNQL
HHHHCCC
>Mature Secondary Structure
MKSYELITILGPTASGKTTFAAALAAQLDTEIISADSRQIYRSMDIGTGKDLADYNVNGK
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCC
QIPYHLIDICEPGYKYNVFEYQHDFFRVYEDMKRRGKLPILCGGTGMYIEAVLKGYKLLD
CCCHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHCCCEEEE
VPQNPELRESLRNKTLEELETILASYKILHNKTDVDTAQRAIRAIEIEEYYKTQAPDVNE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
YNPINSLIIGIHIDRELRREKISRRLRTRLDEGMVDEVRTILATGVKPEDLIYYGLEYKF
CCCHHHEEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHEEEECCCEEE
LTLYIIGELSFEEMVSQLEIAIHQFAKRQMTWFRGMERRGCEIHWIDATLPTEEKIATTM
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHH
RILNNQL
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA