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The map label for this gene is apbE [H]

Identifier: 150006840

GI number: 150006840

Start: 181331

End: 182362

Strand: Reverse

Name: apbE [H]

Synonym: BDI_0171

Alternate gene names: 150006840

Gene position: 182362-181331 (Counterclockwise)

Preceding gene: 150006841

Following gene: 150006839

Centisome position: 3.79

GC content: 43.41

Gene sequence:

>1032_bases
ATGATGCGCAAGAATATTACCATATTAGTCAGCCTGTTGATCTTATCGCTTACGGCTTGCTCGGACAAGGGCCAATATTT
CGAAGAGAGTGGTTCTGTTTTTCATACAAGTTACCACATCAAATACAAGGCCAAGCAAATCCTAACCGATAAGATCGACA
GCGAGCTGCAACGTTTTAACCTCTCCTTAAATCCTTTCAACCCCAACTCCACGATCGCGAAAGTCAATAATAACGAGGAT
GTGGAGGTAGACGAATGGTTTACCGAAGTATTCAATAAGGCGGAAGAAATCTCCCAAAAATCAGGAGGAGCTTTTGATAT
TACTTGTGCTCCATTAATTAATCTATGGGGATTCGGATTCAGTAAGATGGATAGCGTAACCCCGCAGATGATCGATAGCA
TAAAGGCTTTCGTCGGTTATCAAAAAGTAAGATTGGAAGGAAAGAAAATCATCAAAGAAGATCCCCGGATTCTATTGAAT
TGTTCGTCCATCGCAAAAGGTTATGCTTGCGATGTCATCGCCCGCCTGCTGGAAAAGGAAGGCGTAAAGAACTACATGGT
AGAAATAGGTGGTGAGGTTACCATGAAAGGCGTAAATCAACAAGGTGACTGTTGGCGAGTCGGCATCAACAAGCCGGAAA
TAGGGACAAGTGGCGTAACAAATGATGTTGAAGAGATCGTACAATTATGCCAAAAAGGGGGGGTGGCTACTTCTGGTGAC
TACCGAAACTTCTACATCAAGGATGGTAAGAAATATGCCCATACGATCAATCCAGCGACCGGTTATCCCGCTGGACAAAA
TATCCTTAGCGCGACAATCGTGGCGGAAGATTGTATGACAGCAGACGCTTACGCTACGACTTTCATGGTTTTGGGAGTCG
AGAAAGCGAAGCTACTGGCACAATCCATCCCCCAAATAGAGTATTTCATTATTTATGCGGACAACAACGGACAACAGAAA
GTCACCTACTCAAAAGGTATGTTGGAATACCTTCCTAACAGAAAAACATTGGCTATCCTCGAAAATCCTTAA

Upstream 100 bases:

>100_bases
AAAAAAGGAATCCTTGATATTTATACACACAATAGCGTCTCCCCCATGAAAGGGGCATATATGGAATTTTTTATGTAAGT
TCGCGACATAAAATTTCATA

Downstream 100 bases:

>100_bases
GTTCCTACATGAAGCGTTATCTAAATCAACTTGAATGCAAAATACTGAGAGCCGGAGATTCCCTGTTTAATACCACAACC
ACGGATAACCTATTAATCTT

Product: thiamine biosynthesis lipoprotein ApbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MMRKNITILVSLLILSLTACSDKGQYFEESGSVFHTSYHIKYKAKQILTDKIDSELQRFNLSLNPFNPNSTIAKVNNNED
VEVDEWFTEVFNKAEEISQKSGGAFDITCAPLINLWGFGFSKMDSVTPQMIDSIKAFVGYQKVRLEGKKIIKEDPRILLN
CSSIAKGYACDVIARLLEKEGVKNYMVEIGGEVTMKGVNQQGDCWRVGINKPEIGTSGVTNDVEEIVQLCQKGGVATSGD
YRNFYIKDGKKYAHTINPATGYPAGQNILSATIVAEDCMTADAYATTFMVLGVEKAKLLAQSIPQIEYFIIYADNNGQQK
VTYSKGMLEYLPNRKTLAILENP

Sequences:

>Translated_343_residues
MMRKNITILVSLLILSLTACSDKGQYFEESGSVFHTSYHIKYKAKQILTDKIDSELQRFNLSLNPFNPNSTIAKVNNNED
VEVDEWFTEVFNKAEEISQKSGGAFDITCAPLINLWGFGFSKMDSVTPQMIDSIKAFVGYQKVRLEGKKIIKEDPRILLN
CSSIAKGYACDVIARLLEKEGVKNYMVEIGGEVTMKGVNQQGDCWRVGINKPEIGTSGVTNDVEEIVQLCQKGGVATSGD
YRNFYIKDGKKYAHTINPATGYPAGQNILSATIVAEDCMTADAYATTFMVLGVEKAKLLAQSIPQIEYFIIYADNNGQQK
VTYSKGMLEYLPNRKTLAILENP
>Mature_343_residues
MMRKNITILVSLLILSLTACSDKGQYFEESGSVFHTSYHIKYKAKQILTDKIDSELQRFNLSLNPFNPNSTIAKVNNNED
VEVDEWFTEVFNKAEEISQKSGGAFDITCAPLINLWGFGFSKMDSVTPQMIDSIKAFVGYQKVRLEGKKIIKEDPRILLN
CSSIAKGYACDVIARLLEKEGVKNYMVEIGGEVTMKGVNQQGDCWRVGINKPEIGTSGVTNDVEEIVQLCQKGGVATSGD
YRNFYIKDGKKYAHTINPATGYPAGQNILSATIVAEDCMTADAYATTFMVLGVEKAKLLAQSIPQIEYFIIYADNNGQQK
VTYSKGMLEYLPNRKTLAILENP

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=267, Percent_Identity=35.5805243445693, Blast_Score=175, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 38118; Mature: 38118

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRKNITILVSLLILSLTACSDKGQYFEESGSVFHTSYHIKYKAKQILTDKIDSELQRFN
CCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHC
LSLNPFNPNSTIAKVNNNEDVEVDEWFTEVFNKAEEISQKSGGAFDITCAPLINLWGFGF
CEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCC
SKMDSVTPQMIDSIKAFVGYQKVRLEGKKIIKEDPRILLNCSSIAKGYACDVIARLLEKE
HHHHCCCHHHHHHHHHHHCHHHEEECCHHHHCCCCCEEEEHHHHHCCHHHHHHHHHHHHC
GVKNYMVEIGGEVTMKGVNQQGDCWRVGINKPEIGTSGVTNDVEEIVQLCQKGGVATSGD
CHHHHHHHCCCEEEEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
YRNFYIKDGKKYAHTINPATGYPAGQNILSATIVAEDCMTADAYATTFMVLGVEKAKLLA
CCEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHEECHHHHHHHH
QSIPQIEYFIIYADNNGQQKVTYSKGMLEYLPNRKTLAILENP
HCCCCEEEEEEEECCCCCEEEEHHCCHHHHCCCCCEEEEEECC
>Mature Secondary Structure
MMRKNITILVSLLILSLTACSDKGQYFEESGSVFHTSYHIKYKAKQILTDKIDSELQRFN
CCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHC
LSLNPFNPNSTIAKVNNNEDVEVDEWFTEVFNKAEEISQKSGGAFDITCAPLINLWGFGF
CEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCC
SKMDSVTPQMIDSIKAFVGYQKVRLEGKKIIKEDPRILLNCSSIAKGYACDVIARLLEKE
HHHHCCCHHHHHHHHHHHCHHHEEECCHHHHCCCCCEEEEHHHHHCCHHHHHHHHHHHHC
GVKNYMVEIGGEVTMKGVNQQGDCWRVGINKPEIGTSGVTNDVEEIVQLCQKGGVATSGD
CHHHHHHHCCCEEEEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
YRNFYIKDGKKYAHTINPATGYPAGQNILSATIVAEDCMTADAYATTFMVLGVEKAKLLA
CCEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHEECHHHHHHHH
QSIPQIEYFIIYADNNGQQKVTYSKGMLEYLPNRKTLAILENP
HCCCCEEEEEEEECCCCCEEEEHHCCHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]