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The map label for this gene is manA [H]

Identifier: 150006734

GI number: 150006734

Start: 62679

End: 63785

Strand: Reverse

Name: manA [H]

Synonym: BDI_0060

Alternate gene names: 150006734

Gene position: 63785-62679 (Counterclockwise)

Preceding gene: 150006736

Following gene: 150006733

Centisome position: 1.33

GC content: 47.79

Gene sequence:

>1107_bases
ATGAAGAACACGAAATTAATTACCATGTTATGCCTAGGGGCACTAATCGCCTGTTCACCGTCGAAGAAAGGACTAGAACC
GACAAGCGAACAGGTTTCGCCGAGAGAACGTTTAATAGAGAACCTACGTGCGATTCCACAAAAAGGAATCATGTTCGGAC
ATCACGATGATACGGTTTATGGCATCGGATGGGAATTTGACGAAGGAGGCTCAGACGTGCAAAAAGTATGCGGCGATTAT
CCGGCCGTTATCAGCTTTGATCTTGGAGAGATCGAACTCGGGGGAGATAAAAGCCTTGATAAAGTACCTTTCGAGAAAAT
ACGCAAGGAAATCATCAACCAATATAACCGGGGAGGCTTGGTATCTTTAAGTTGGCATCCCCGTAACCCTAAAACCGGAG
GGGACGCTTGGGACGTATCAGACCATGCTGTCGTGAAATCGATCCTTCCGGAAGGGGAGAACTATGAGAAATTCCAAAGT
TGGCTAGGCAAAGTGAATGATTTCATCCTTTCCTTGAAAACAAGCGATAGCACCAAGATTCCCGTCCTATTCCGTCCTTG
GCACGAACACACCGGAAGTTGGTTCTGGTGGGGACAGAATTTATGTACCACGGATGAATACAAGGCGCTATGGCGAATGA
CCGCCGATTATCTAAACGCTCACGGAGCGGCAGACCAGATCGTTTACGCTTATTCCACAGGTACGGAGCCGCGGGATCAA
GCGTCTTATCTGGAACGTTACCCCGGACATGACTTGATCGACGTCCTAGGCTTCGACGCTTACCAACGGGAAGATAAGGA
TTTCTTCTTGAAAAGCATGGATACCAGTCTTTCCGTAATAGACAGCATAGGCAAAGCCAACCATAAGATCATAGCGATCA
CGGAGACCGGCTACGAAGGGGTACCAGACTCCACATGGTGGACGGGAACCCTGCTGCCTGCCATGGAGAAATATCCGGTC
GCTTATGTATTGGTATGGAGAAACGCCCGTGAAAAAGTCACTCACTTCTTTGGCCCTTATCCCGGACAAGCCTCTGCCGA
GGATTTCAAGAAGTTCTTTGAAAATCCGAAAACATTGTTCGCCTCCGATATCAACCTATATAAATAA

Upstream 100 bases:

>100_bases
TTCCGGTTAAAATAAAAATAATATCGCTATCCGATAAAATAATACTAGATTGTCTGCCCGATACCTCTTACATTCGCGTT
CATAAAGAAAGACAGTTATC

Downstream 100 bases:

>100_bases
CATCATACATAAACCAGATAAATCACGTCATATCTAGTTGTAGAGACGGGGCGTGCCCCGTCTCCCACCATCTGATAATA
TCATTTAAAGCAAAAACATC

Product: beta-glycosidase

Products: NA

Alternate protein names: Mannanase A [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKNTKLITMLCLGALIACSPSKKGLEPTSEQVSPRERLIENLRAIPQKGIMFGHHDDTVYGIGWEFDEGGSDVQKVCGDY
PAVISFDLGEIELGGDKSLDKVPFEKIRKEIINQYNRGGLVSLSWHPRNPKTGGDAWDVSDHAVVKSILPEGENYEKFQS
WLGKVNDFILSLKTSDSTKIPVLFRPWHEHTGSWFWWGQNLCTTDEYKALWRMTADYLNAHGAADQIVYAYSTGTEPRDQ
ASYLERYPGHDLIDVLGFDAYQREDKDFFLKSMDTSLSVIDSIGKANHKIIAITETGYEGVPDSTWWTGTLLPAMEKYPV
AYVLVWRNAREKVTHFFGPYPGQASAEDFKKFFENPKTLFASDINLYK

Sequences:

>Translated_368_residues
MKNTKLITMLCLGALIACSPSKKGLEPTSEQVSPRERLIENLRAIPQKGIMFGHHDDTVYGIGWEFDEGGSDVQKVCGDY
PAVISFDLGEIELGGDKSLDKVPFEKIRKEIINQYNRGGLVSLSWHPRNPKTGGDAWDVSDHAVVKSILPEGENYEKFQS
WLGKVNDFILSLKTSDSTKIPVLFRPWHEHTGSWFWWGQNLCTTDEYKALWRMTADYLNAHGAADQIVYAYSTGTEPRDQ
ASYLERYPGHDLIDVLGFDAYQREDKDFFLKSMDTSLSVIDSIGKANHKIIAITETGYEGVPDSTWWTGTLLPAMEKYPV
AYVLVWRNAREKVTHFFGPYPGQASAEDFKKFFENPKTLFASDINLYK
>Mature_368_residues
MKNTKLITMLCLGALIACSPSKKGLEPTSEQVSPRERLIENLRAIPQKGIMFGHHDDTVYGIGWEFDEGGSDVQKVCGDY
PAVISFDLGEIELGGDKSLDKVPFEKIRKEIINQYNRGGLVSLSWHPRNPKTGGDAWDVSDHAVVKSILPEGENYEKFQS
WLGKVNDFILSLKTSDSTKIPVLFRPWHEHTGSWFWWGQNLCTTDEYKALWRMTADYLNAHGAADQIVYAYSTGTEPRDQ
ASYLERYPGHDLIDVLGFDAYQREDKDFFLKSMDTSLSVIDSIGKANHKIIAITETGYEGVPDSTWWTGTLLPAMEKYPV
AYVLVWRNAREKVTHFFGPYPGQASAEDFKKFFENPKTLFASDINLYK

Specific function: Hydrolyzes mannan and galactomannan, but displays little activity towards other polysaccharides located in the plant cell wall. Appears to act in synergy with alpha-galactosidase (AgaA) to elicit hydrolysis of galactomannan. Preferentially hydrolyzes the

COG id: COG4124

COG function: function code G; Beta-mannanase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 26 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022790
- InterPro:   IPR000805
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF02156 Glyco_hydro_26 [H]

EC number: =3.2.1.78 [H]

Molecular weight: Translated: 41686; Mature: 41686

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTKLITMLCLGALIACSPSKKGLEPTSEQVSPRERLIENLRAIPQKGIMFGHHDDTVY
CCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCHHCCEEECCCCEEE
GIGWEFDEGGSDVQKVCGDYPAVISFDLGEIELGGDKSLDKVPFEKIRKEIINQYNRGGL
EECEEECCCCHHHHHHHCCCCCEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCE
VSLSWHPRNPKTGGDAWDVSDHAVVKSILPEGENYEKFQSWLGKVNDFILSLKTSDSTKI
EEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCC
PVLFRPWHEHTGSWFWWGQNLCTTDEYKALWRMTADYLNAHGAADQIVYAYSTGTEPRDQ
CEEEECHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHH
ASYLERYPGHDLIDVLGFDAYQREDKDFFLKSMDTSLSVIDSIGKANHKIIAITETGYEG
HHHHHHCCCCHHHHHHCCCHHHCCCHHHHHHHHCHHHHHHHHHCCCCCEEEEEEECCCCC
VPDSTWWTGTLLPAMEKYPVAYVLVWRNAREKVTHFFGPYPGQASAEDFKKFFENPKTLF
CCCCCCCCCCHHHHHHHCCEEEEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEE
ASDINLYK
ECCCCCCC
>Mature Secondary Structure
MKNTKLITMLCLGALIACSPSKKGLEPTSEQVSPRERLIENLRAIPQKGIMFGHHDDTVY
CCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCHHCCEEECCCCEEE
GIGWEFDEGGSDVQKVCGDYPAVISFDLGEIELGGDKSLDKVPFEKIRKEIINQYNRGGL
EECEEECCCCHHHHHHHCCCCCEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCE
VSLSWHPRNPKTGGDAWDVSDHAVVKSILPEGENYEKFQSWLGKVNDFILSLKTSDSTKI
EEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCC
PVLFRPWHEHTGSWFWWGQNLCTTDEYKALWRMTADYLNAHGAADQIVYAYSTGTEPRDQ
CEEEECHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHH
ASYLERYPGHDLIDVLGFDAYQREDKDFFLKSMDTSLSVIDSIGKANHKIIAITETGYEG
HHHHHHCCCCHHHHHHCCCHHHCCCHHHHHHHHCHHHHHHHHHCCCCCEEEEEEECCCCC
VPDSTWWTGTLLPAMEKYPVAYVLVWRNAREKVTHFFGPYPGQASAEDFKKFFENPKTLF
CCCCCCCCCCHHHHHHHCCEEEEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEE
ASDINLYK
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7848261 [H]