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The map label for this gene is hsdR [H]

Identifier: 150006643

GI number: 150006643

Start: 5131887

End: 5134589

Strand: Reverse

Name: hsdR [H]

Synonym: BVU_4164

Alternate gene names: 150006643

Gene position: 5134589-5131887 (Counterclockwise)

Preceding gene: 150006644

Following gene: 150006642

Centisome position: 99.45

GC content: 41.99

Gene sequence:

>2703_bases
ATGACTCCTGAAGAAAAAGCAAGAATAAAGATAGACCAGTGGTTTGCCGATGCTGGTTGGAAAGTAGTCAACAGGGAGGA
TTATGAACCTACTTGTACGGCTGTAGCCATAAGAGAAGGTTTATTGAAAGGAAATCTTGAGGCAGATTATTTCCTTTTCA
TTAATGGAAAAGCAGTAGGGGTACTTGAAGCTAAGCGTGAAGAAACGGATGCTTTTGCTTCAGAGGTATGCGAGCAAGCA
GCCTTATATGCAAGAAGTGTTCCGAATATCTATCAGGCATATCAAAAGCCATTGCCTTTTATCTTCACTTCGAATGGTAA
GGAACTATATTGCTGTGATTTTCGTGAACAAGATTCTTGTTTCAGGCAGATCATGAATATCCCTACACCACATGAATTGG
TAAAGAGGCTGGGAATTGAAGATGCATTTGCCGGACTCCCTACATTGAAAAAGAAAGGGCTGCGTGATTGCCAATATGAG
GCAGTGACAGAACTTGAAAAGAGTTTCCGTGCTGGGCAAAATCGGGCGTTGATGGTTCTTGCCACCGGTGTGGGTAAAAC
ATATACTGCTTGTCTTGCTGCCTACCGGATGCTTTCTTATACACCAATGCGTAGAGTGTTGTTTCTTGTAGACCGAAATA
ATCTTGGAAAACAAGCAGAAGGAGAATTTGGAACTTTCCGTCTGACAGAGAACGGGGAGGCTTTCAATACTATCTTTACG
GTCAATCGCCTTCGCTCGTCTTCTATTCCTTCTGATAGCAATGTCGTTATTTCTACAATACAACGTCTATTCTCATTCTT
GAAAGGAGAAACTATTGAGGATAATGATGATGACGAGAATGAACCTATAGAGGAAGTAACCTTGCCTCCTAATCCCAACT
TACCGCACGACTACTTTGATATGATTATCATAGACGAGTGCCATCGCTCCATTTATGGGAACTGGCGCAAGGTGCTGGAG
TATTTCGATACGGCAAGATTGGTTGGTCTGACCGCAACTCCTATCCCTGAAACAATGGCATTCTTCAATAATAACTGCAT
TGTTAACTACACATTGGAAAAGAGTATTGTTGATGGCGTGAATGTGGATTGTCGGGTATATAGAATAAAAACGCAAGTCA
CAGAAACGGGTGGGGCTATATTAGAGGGTGAGAAATTTAAAGAAGAAACAAGATATACAGGTGAGGTTAAGATTGTAAGT
AGTAAGGAAACCAAAATCTATACAAATAAAGAACTTAATCGGAGTATTATCAATCCGGCACAGATAAAGTTAGTCCTTTC
GACCTATCGGGATGTGGTTTATACGGAATTGTTTAATGACCCGCAACGTGAACCAAATATGGACTTTTTGCCCAAAACAT
TGATTTTTGCTCTCAATGAAGCTCATGCTACAAATATCGTACAAATAGCTAAAGAGGTGTTTGGACGGACTGATGACCGC
TTTGTTCAGAAAATTACCTATTCGGCAGGCGACAGTAACGAACTGATACGCCAATTCCGTAACGACAAGGATTTCCGTAT
TGCTGTCACCTGTACGTTGGTTGCCACCGGAACAGATGTAAAGCCGCTGGAAGTGGTGATGTTCATGCGTGATGTGGAGT
CATTGCCTTTGTATATTCAGATGAAAGGGCGTGGAGTACGTACTATTGGTGATGAGCAACTTCGCAATGTTACCCCGAAC
GCATTCAGCAAGGATTGTTTTTATTTGGTTGATGCGGTAGGCGTGACGGAACATGCGCAAACAGTGGCACCTATAGATGA
TGCTCCTACAACAAAGACGATTACTCTAAAGGAGCTATTAGAACGTATTAGTCATGGCTATATTCCTGACGAGTATCTCA
AACGGCTTGCTGCAACGCTTGCCCGAATATACAACAAGGCGGATGATCCGCAACGGAAAGAGTTTGTCCGTTTGTCTCAT
GATGACATGAAGGAACTTTCTGCCAGAATATACGATGCACTGGAGAAAGGCATTCTTCCACCGTTTGTAAGCACTGATGA
GCCTAACAATGAACGTAAGGGGCTAGTCGCTCCGCTTGCCAATCATGCAGATGCTCGGAAATACCTTCTTATACTTGCAG
CCGGATTCGTCAATACATTGATGCCGGGTGAAGATACGCTTATCTCCAAAGGGTTTTCCATTGAGGAAGCAAAAAATACG
ACAGAGGCTTTTGAAGATTTTTGTAAAAAGTACTATGACGAAATAGAGGCACTGCGCATCATTTACAACAATGAGGGAGA
GCCTATAACCTATTCGATGCTAAAGGATTTGGAGAACAGACTTAAAATGGCAAACAATCATTTTACATCCAAACAACTCT
GGAACTCTTATGCCATAGTTAACCCTAAAGTGGTAAGGCGTTCCATTACCAAAGAAGAAAGCGATGCATTGACCAATATC
ATACAACTTGTGCGCTTTGCTTTCCACCAAATCGAACGATTGGATAGCGTAGTTACTACCTCTAAACAGTTTTTCAATCT
TTGGTTAGGGCAAAACCAGCGTGAAATAACTGACAAACAGCGAGAGGTTATCAGTCGTATTGTGGATTACATCGCTTCTA
ACGGAGCTTGTACTATCAGAGATATTCGTGAAGATGATGCTACCCATGCAGCCCAAATGATTAGAGCGTTTGGCAATATG
CAGAAGGCAGACGAGGCACTTCATTCACTTTACACATTTGTAGTTTTAAGAAAAGCAGCATAA

Upstream 100 bases:

>100_bases
GTTGCAATAAATTATTTAATACGGTTAGGAGGTTCTGGTTCCTTTTGTAACCTGTTGCAGATGTGCCTATGAACTTCCGA
TAATTTACAATATCAAGAAC

Downstream 100 bases:

>100_bases
AGAAATGGCAACAAATAATACAACAGAGCAATCGCTCACCAAAAAAGTATGGAATCTGGCAACCACTCTTGCCGGACAAG
GAATTGGATTTACTGATTAT

Product: type I restriction enzyme EcoKI R protein

Products: NA

Alternate protein names: R.EcoKI [H]

Number of amino acids: Translated: 900; Mature: 899

Protein sequence:

>900_residues
MTPEEKARIKIDQWFADAGWKVVNREDYEPTCTAVAIREGLLKGNLEADYFLFINGKAVGVLEAKREETDAFASEVCEQA
ALYARSVPNIYQAYQKPLPFIFTSNGKELYCCDFREQDSCFRQIMNIPTPHELVKRLGIEDAFAGLPTLKKKGLRDCQYE
AVTELEKSFRAGQNRALMVLATGVGKTYTACLAAYRMLSYTPMRRVLFLVDRNNLGKQAEGEFGTFRLTENGEAFNTIFT
VNRLRSSSIPSDSNVVISTIQRLFSFLKGETIEDNDDDENEPIEEVTLPPNPNLPHDYFDMIIIDECHRSIYGNWRKVLE
YFDTARLVGLTATPIPETMAFFNNNCIVNYTLEKSIVDGVNVDCRVYRIKTQVTETGGAILEGEKFKEETRYTGEVKIVS
SKETKIYTNKELNRSIINPAQIKLVLSTYRDVVYTELFNDPQREPNMDFLPKTLIFALNEAHATNIVQIAKEVFGRTDDR
FVQKITYSAGDSNELIRQFRNDKDFRIAVTCTLVATGTDVKPLEVVMFMRDVESLPLYIQMKGRGVRTIGDEQLRNVTPN
AFSKDCFYLVDAVGVTEHAQTVAPIDDAPTTKTITLKELLERISHGYIPDEYLKRLAATLARIYNKADDPQRKEFVRLSH
DDMKELSARIYDALEKGILPPFVSTDEPNNERKGLVAPLANHADARKYLLILAAGFVNTLMPGEDTLISKGFSIEEAKNT
TEAFEDFCKKYYDEIEALRIIYNNEGEPITYSMLKDLENRLKMANNHFTSKQLWNSYAIVNPKVVRRSITKEESDALTNI
IQLVRFAFHQIERLDSVVTTSKQFFNLWLGQNQREITDKQREVISRIVDYIASNGACTIRDIREDDATHAAQMIRAFGNM
QKADEALHSLYTFVVLRKAA

Sequences:

>Translated_900_residues
MTPEEKARIKIDQWFADAGWKVVNREDYEPTCTAVAIREGLLKGNLEADYFLFINGKAVGVLEAKREETDAFASEVCEQA
ALYARSVPNIYQAYQKPLPFIFTSNGKELYCCDFREQDSCFRQIMNIPTPHELVKRLGIEDAFAGLPTLKKKGLRDCQYE
AVTELEKSFRAGQNRALMVLATGVGKTYTACLAAYRMLSYTPMRRVLFLVDRNNLGKQAEGEFGTFRLTENGEAFNTIFT
VNRLRSSSIPSDSNVVISTIQRLFSFLKGETIEDNDDDENEPIEEVTLPPNPNLPHDYFDMIIIDECHRSIYGNWRKVLE
YFDTARLVGLTATPIPETMAFFNNNCIVNYTLEKSIVDGVNVDCRVYRIKTQVTETGGAILEGEKFKEETRYTGEVKIVS
SKETKIYTNKELNRSIINPAQIKLVLSTYRDVVYTELFNDPQREPNMDFLPKTLIFALNEAHATNIVQIAKEVFGRTDDR
FVQKITYSAGDSNELIRQFRNDKDFRIAVTCTLVATGTDVKPLEVVMFMRDVESLPLYIQMKGRGVRTIGDEQLRNVTPN
AFSKDCFYLVDAVGVTEHAQTVAPIDDAPTTKTITLKELLERISHGYIPDEYLKRLAATLARIYNKADDPQRKEFVRLSH
DDMKELSARIYDALEKGILPPFVSTDEPNNERKGLVAPLANHADARKYLLILAAGFVNTLMPGEDTLISKGFSIEEAKNT
TEAFEDFCKKYYDEIEALRIIYNNEGEPITYSMLKDLENRLKMANNHFTSKQLWNSYAIVNPKVVRRSITKEESDALTNI
IQLVRFAFHQIERLDSVVTTSKQFFNLWLGQNQREITDKQREVISRIVDYIASNGACTIRDIREDDATHAAQMIRAFGNM
QKADEALHSLYTFVVLRKAA
>Mature_899_residues
TPEEKARIKIDQWFADAGWKVVNREDYEPTCTAVAIREGLLKGNLEADYFLFINGKAVGVLEAKREETDAFASEVCEQAA
LYARSVPNIYQAYQKPLPFIFTSNGKELYCCDFREQDSCFRQIMNIPTPHELVKRLGIEDAFAGLPTLKKKGLRDCQYEA
VTELEKSFRAGQNRALMVLATGVGKTYTACLAAYRMLSYTPMRRVLFLVDRNNLGKQAEGEFGTFRLTENGEAFNTIFTV
NRLRSSSIPSDSNVVISTIQRLFSFLKGETIEDNDDDENEPIEEVTLPPNPNLPHDYFDMIIIDECHRSIYGNWRKVLEY
FDTARLVGLTATPIPETMAFFNNNCIVNYTLEKSIVDGVNVDCRVYRIKTQVTETGGAILEGEKFKEETRYTGEVKIVSS
KETKIYTNKELNRSIINPAQIKLVLSTYRDVVYTELFNDPQREPNMDFLPKTLIFALNEAHATNIVQIAKEVFGRTDDRF
VQKITYSAGDSNELIRQFRNDKDFRIAVTCTLVATGTDVKPLEVVMFMRDVESLPLYIQMKGRGVRTIGDEQLRNVTPNA
FSKDCFYLVDAVGVTEHAQTVAPIDDAPTTKTITLKELLERISHGYIPDEYLKRLAATLARIYNKADDPQRKEFVRLSHD
DMKELSARIYDALEKGILPPFVSTDEPNNERKGLVAPLANHADARKYLLILAAGFVNTLMPGEDTLISKGFSIEEAKNTT
EAFEDFCKKYYDEIEALRIIYNNEGEPITYSMLKDLENRLKMANNHFTSKQLWNSYAIVNPKVVRRSITKEESDALTNII
QLVRFAFHQIERLDSVVTTSKQFFNLWLGQNQREITDKQREVISRIVDYIASNGACTIRDIREDDATHAAQMIRAFGNMQ
KADEALHSLYTFVVLRKAA

Specific function: The EcoKI enzyme recognizes 5'-AACN(6)GTGC-3'. Subunit R is required for both nuclease and ATPase activities, but not for modification [H]

COG id: COG4096

COG function: function code V; Type I site-specific restriction-modification system, R (restriction) subunit and related helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI226510991, Length=686, Percent_Identity=26.9679300291545, Blast_Score=182, Evalue=8e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR021943
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR007409 [H]

Pfam domain/function: PF12082 DUF3559; PF00271 Helicase_C; PF04313 HSDR_N; PF04851 ResIII [H]

EC number: =3.1.21.3 [H]

Molecular weight: Translated: 102529; Mature: 102398

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPEEKARIKIDQWFADAGWKVVNREDYEPTCTAVAIREGLLKGNLEADYFLFINGKAVG
CCCCCCCCEEHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEE
VLEAKREETDAFASEVCEQAALYARSVPNIYQAYQKPLPFIFTSNGKELYCCDFREQDSC
EEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEECCCCEEEEECCCCHHHH
FRQIMNIPTPHELVKRLGIEDAFAGLPTLKKKGLRDCQYEAVTELEKSFRAGQNRALMVL
HHHHHCCCCHHHHHHHCCCHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEE
ATGVGKTYTACLAAYRMLSYTPMRRVLFLVDRNNLGKQAEGEFGTFRLTENGEAFNTIFT
EECCCHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHH
VNRLRSSSIPSDSNVVISTIQRLFSFLKGETIEDNDDDENEPIEEVTLPPNPNLPHDYFD
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHEEECCCCCCCCHHHHH
MIIIDECHRSIYGNWRKVLEYFDTARLVGLTATPIPETMAFFNNNCIVNYTLEKSIVDGV
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCCCEEEEEEHHHHHCCC
NVDCRVYRIKTQVTETGGAILEGEKFKEETRYTGEVKIVSSKETKIYTNKELNRSIINPA
CCCEEEEEEEEEEECCCCCEECCHHHHHHCCCCCEEEEEECCCCEEEECCHHHHHHCCHH
QIKLVLSTYRDVVYTELFNDPQREPNMDFLPKTLIFALNEAHATNIVQIAKEVFGRTDDR
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
FVQKITYSAGDSNELIRQFRNDKDFRIAVTCTLVATGTDVKPLEVVMFMRDVESLPLYIQ
HHHHHHCCCCCHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEE
MKGRGVRTIGDEQLRNVTPNAFSKDCFYLVDAVGVTEHAQTVAPIDDAPTTKTITLKELL
ECCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEHHHHH
ERISHGYIPDEYLKRLAATLARIYNKADDPQRKEFVRLSHDDMKELSARIYDALEKGILP
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
PFVSTDEPNNERKGLVAPLANHADARKYLLILAAGFVNTLMPGEDTLISKGFSIEEAKNT
CCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHH
TEAFEDFCKKYYDEIEALRIIYNNEGEPITYSMLKDLENRLKMANNHFTSKQLWNSYAIV
HHHHHHHHHHHHHHHHHEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHCCEEEC
NPKVVRRSITKEESDALTNIIQLVRFAFHQIERLDSVVTTSKQFFNLWLGQNQREITDKQ
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
REVISRIVDYIASNGACTIRDIREDDATHAAQMIRAFGNMQKADEALHSLYTFVVLRKAA
HHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPEEKARIKIDQWFADAGWKVVNREDYEPTCTAVAIREGLLKGNLEADYFLFINGKAVG
CCCCCCCEEHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEE
VLEAKREETDAFASEVCEQAALYARSVPNIYQAYQKPLPFIFTSNGKELYCCDFREQDSC
EEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEECCCCEEEEECCCCHHHH
FRQIMNIPTPHELVKRLGIEDAFAGLPTLKKKGLRDCQYEAVTELEKSFRAGQNRALMVL
HHHHHCCCCHHHHHHHCCCHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEE
ATGVGKTYTACLAAYRMLSYTPMRRVLFLVDRNNLGKQAEGEFGTFRLTENGEAFNTIFT
EECCCHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHH
VNRLRSSSIPSDSNVVISTIQRLFSFLKGETIEDNDDDENEPIEEVTLPPNPNLPHDYFD
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHEEECCCCCCCCHHHHH
MIIIDECHRSIYGNWRKVLEYFDTARLVGLTATPIPETMAFFNNNCIVNYTLEKSIVDGV
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCCCEEEEEEHHHHHCCC
NVDCRVYRIKTQVTETGGAILEGEKFKEETRYTGEVKIVSSKETKIYTNKELNRSIINPA
CCCEEEEEEEEEEECCCCCEECCHHHHHHCCCCCEEEEEECCCCEEEECCHHHHHHCCHH
QIKLVLSTYRDVVYTELFNDPQREPNMDFLPKTLIFALNEAHATNIVQIAKEVFGRTDDR
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
FVQKITYSAGDSNELIRQFRNDKDFRIAVTCTLVATGTDVKPLEVVMFMRDVESLPLYIQ
HHHHHHCCCCCHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEE
MKGRGVRTIGDEQLRNVTPNAFSKDCFYLVDAVGVTEHAQTVAPIDDAPTTKTITLKELL
ECCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEHHHHH
ERISHGYIPDEYLKRLAATLARIYNKADDPQRKEFVRLSHDDMKELSARIYDALEKGILP
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
PFVSTDEPNNERKGLVAPLANHADARKYLLILAAGFVNTLMPGEDTLISKGFSIEEAKNT
CCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHH
TEAFEDFCKKYYDEIEALRIIYNNEGEPITYSMLKDLENRLKMANNHFTSKQLWNSYAIV
HHHHHHHHHHHHHHHHHEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHCCEEEC
NPKVVRRSITKEESDALTNIIQLVRFAFHQIERLDSVVTTSKQFFNLWLGQNQREITDKQ
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
REVISRIVDYIASNGACTIRDIREDDATHAAQMIRAFGNMQKADEALHSLYTFVVLRKAA
HHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3323532; 7610040; 9278503; 1650347 [H]