Click here to switch to the map view.

The map label for this gene is hsdM [C]

Identifier: 150006642

GI number: 150006642

Start: 5130464

End: 5131882

Strand: Reverse

Name: hsdM [C]

Synonym: BVU_4163

Alternate gene names: 150006642

Gene position: 5131882-5130464 (Counterclockwise)

Preceding gene: 150006643

Following gene: 150006641

Centisome position: 99.39

GC content: 41.23

Gene sequence:

>1419_bases
ATGGCAACAAATAATACAACAGAGCAATCGCTCACCAAAAAAGTATGGAATCTGGCAACCACTCTTGCCGGACAAGGAAT
TGGATTTACTGATTATATTACCCAACTGACCTATCTTTTATTCTTGAAGATGGATGCCGAAAATGTAGAAATGTTTGGAG
AAGAGTCGGCTATTCCAACTGGTTATCAGTGGGCAGATTTGATAGTACTTGATGGCTTGGATTTGGTAAAACAATATGAA
GAGACCCTTAAATTGCTTAGTGAGCAGGATAATCTGATAGGTACGATTTATACGAAGGCTCAAAACAAGATAGACAAGCC
TGTTTATCTGAAAAAGGTCATCACCATGATTGATGAGGAGCAATGGCTCATTATGGACGGTGATGTGAAAGGAGCTATCT
ACGAAAGCATTCTTGAAAAGAACGGACAAGACAAGAAAAGCGGGGCAGGACAGTATTTTACTCCTCGTCCACTTATTCAG
GCAATAGTAGATTGTATCAATCCGCAAATGGGGGAAACGGTTTGTGACCCGGCTTGTGGAACGGGTGGATTCTTGCTTAC
AGCTTACGATTATATGAAAGGTCAGTCAGCAAGTAAAGAAAAACGTGATTTCTTGCGTGACAAAGCTTTGCATGGTGTGG
ATAATACGCCTTTAGTGGTGACGCTTGCTTCCATGAATCTTTATTTGCATGGTATTGGTACAGACCGTAGTCCGATTGTT
TGTGAGGATTCTTTGGAAAAAGAACCGTCAACTCTTGTAGATGTGATTCTTGCCAATCCTCCTTTTGGAACTCGTCCGGC
TGGATCGGTGGATATTAACCGCCCGGATTTTTATGTAGAGACCAAGAATAACCAGTTAAATTTCCTCCAGCACATGATGC
TTATGCTCAAAACGGGAGGACGTGCAGCAGTTGTACTTCCTGATAATGTTCTCTTTGAGGCTGGAGCAGGTGAGACTATT
CGTAAACGCTTATTGCAGGATTTTAATTTGCATACTATCTTGCGTTTACCTACAGGTATTTTCTATGCCCAAGGCGTAAA
AGCCAACGTGTTGTTTTTCAGCAAAGGACAACCGACCAAGGAAATATGGTTTTACGACTACCGTACAGATATAAAGCATA
CGCTTGCCACCAATAAACTGGAACGGCATCATTTGGATGATTTTGTTTCTTGCTATAATAATCGAGTAGAGACATACGAT
GCAGAAAATAATCCGCAAGGTCGTTGGCGTAAATACCCTGTAGATGAAATTCTTACCAGAGACAAAACAAGTCTTGACAT
TACTTGGATAAAGCAAGGTGGTGAGGTGGACAACCGTTCATTGGCAGAGCTGATGGCTGATATAAAGGATAAAAGCCAAA
CGATAAGTGCTGCTGTGGTTGAACTTGAAAAGCTACTTGCGAATATTGAAGAAAATTGA

Upstream 100 bases:

>100_bases
GCAGCCCAAATGATTAGAGCGTTTGGCAATATGCAGAAGGCAGACGAGGCACTTCATTCACTTTACACATTTGTAGTTTT
AAGAAAAGCAGCATAAAGAA

Downstream 100 bases:

>100_bases
GTTATGGGAAAAGAGGTTTTCACAAATGATAAAGATATAAACGACCTGTTCTTGAAGGTTGTTAACCTTGTTACGCAGGC
AAGGGAACGTGTGGCAACAG

Product: type I restriction enzyme StySJI M protein

Products: NA

Alternate protein names: M.BthVORF4518P [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MATNNTTEQSLTKKVWNLATTLAGQGIGFTDYITQLTYLLFLKMDAENVEMFGEESAIPTGYQWADLIVLDGLDLVKQYE
ETLKLLSEQDNLIGTIYTKAQNKIDKPVYLKKVITMIDEEQWLIMDGDVKGAIYESILEKNGQDKKSGAGQYFTPRPLIQ
AIVDCINPQMGETVCDPACGTGGFLLTAYDYMKGQSASKEKRDFLRDKALHGVDNTPLVVTLASMNLYLHGIGTDRSPIV
CEDSLEKEPSTLVDVILANPPFGTRPAGSVDINRPDFYVETKNNQLNFLQHMMLMLKTGGRAAVVLPDNVLFEAGAGETI
RKRLLQDFNLHTILRLPTGIFYAQGVKANVLFFSKGQPTKEIWFYDYRTDIKHTLATNKLERHHLDDFVSCYNNRVETYD
AENNPQGRWRKYPVDEILTRDKTSLDITWIKQGGEVDNRSLAELMADIKDKSQTISAAVVELEKLLANIEEN

Sequences:

>Translated_472_residues
MATNNTTEQSLTKKVWNLATTLAGQGIGFTDYITQLTYLLFLKMDAENVEMFGEESAIPTGYQWADLIVLDGLDLVKQYE
ETLKLLSEQDNLIGTIYTKAQNKIDKPVYLKKVITMIDEEQWLIMDGDVKGAIYESILEKNGQDKKSGAGQYFTPRPLIQ
AIVDCINPQMGETVCDPACGTGGFLLTAYDYMKGQSASKEKRDFLRDKALHGVDNTPLVVTLASMNLYLHGIGTDRSPIV
CEDSLEKEPSTLVDVILANPPFGTRPAGSVDINRPDFYVETKNNQLNFLQHMMLMLKTGGRAAVVLPDNVLFEAGAGETI
RKRLLQDFNLHTILRLPTGIFYAQGVKANVLFFSKGQPTKEIWFYDYRTDIKHTLATNKLERHHLDDFVSCYNNRVETYD
AENNPQGRWRKYPVDEILTRDKTSLDITWIKQGGEVDNRSLAELMADIKDKSQTISAAVVELEKLLANIEEN
>Mature_471_residues
ATNNTTEQSLTKKVWNLATTLAGQGIGFTDYITQLTYLLFLKMDAENVEMFGEESAIPTGYQWADLIVLDGLDLVKQYEE
TLKLLSEQDNLIGTIYTKAQNKIDKPVYLKKVITMIDEEQWLIMDGDVKGAIYESILEKNGQDKKSGAGQYFTPRPLIQA
IVDCINPQMGETVCDPACGTGGFLLTAYDYMKGQSASKEKRDFLRDKALHGVDNTPLVVTLASMNLYLHGIGTDRSPIVC
EDSLEKEPSTLVDVILANPPFGTRPAGSVDINRPDFYVETKNNQLNFLQHMMLMLKTGGRAAVVLPDNVLFEAGAGETIR
KRLLQDFNLHTILRLPTGIFYAQGVKANVLFFSKGQPTKEIWFYDYRTDIKHTLATNKLERHHLDDFVSCYNNRVETYDA
ENNPQGRWRKYPVDEILTRDKTSLDITWIKQGGEVDNRSLAELMADIKDKSQTISAAVVELEKLLANIEEN

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=508, Percent_Identity=35.6299212598425, Blast_Score=276, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 53120; Mature: 52989

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATNNTTEQSLTKKVWNLATTLAGQGIGFTDYITQLTYLLFLKMDAENVEMFGEESAIPT
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCHHHHCCCCCCCCC
GYQWADLIVLDGLDLVKQYEETLKLLSEQDNLIGTIYTKAQNKIDKPVYLKKVITMIDEE
CCCHHHEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCCHHHHHHHHHHCCC
QWLIMDGDVKGAIYESILEKNGQDKKSGAGQYFTPRPLIQAIVDCINPQMGETVCDPACG
CEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHCCCCCC
TGGFLLTAYDYMKGQSASKEKRDFLRDKALHGVDNTPLVVTLASMNLYLHGIGTDRSPIV
CCCEEEEEHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECEEEEEEECCCCCCEE
CEDSLEKEPSTLVDVILANPPFGTRPAGSVDINRPDFYVETKNNQLNFLQHMMLMLKTGG
ECCCCCCCCCCEEEEEECCCCCCCCCCCCEECCCCCEEEEECCCHHHHHHHHHHHHHCCC
RAAVVLPDNVLFEAGAGETIRKRLLQDFNLHTILRLPTGIFYAQGVKANVLFFSKGQPTK
CEEEEECCCEEEECCCCHHHHHHHHHHCCCEEEEECCCCCEEECCCEEEEEEEECCCCCC
EIWFYDYRTDIKHTLATNKLERHHLDDFVSCYNNRVETYDAENNPQGRWRKYPVDEILTR
EEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHCC
DKTSLDITWIKQGGEVDNRSLAELMADIKDKSQTISAAVVELEKLLANIEEN
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ATNNTTEQSLTKKVWNLATTLAGQGIGFTDYITQLTYLLFLKMDAENVEMFGEESAIPT
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCHHHHCCCCCCCCC
GYQWADLIVLDGLDLVKQYEETLKLLSEQDNLIGTIYTKAQNKIDKPVYLKKVITMIDEE
CCCHHHEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCCHHHHHHHHHHCCC
QWLIMDGDVKGAIYESILEKNGQDKKSGAGQYFTPRPLIQAIVDCINPQMGETVCDPACG
CEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHCCCCCC
TGGFLLTAYDYMKGQSASKEKRDFLRDKALHGVDNTPLVVTLASMNLYLHGIGTDRSPIV
CCCEEEEEHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECEEEEEEECCCCCCEE
CEDSLEKEPSTLVDVILANPPFGTRPAGSVDINRPDFYVETKNNQLNFLQHMMLMLKTGG
ECCCCCCCCCCEEEEEECCCCCCCCCCCCEECCCCCEEEEECCCHHHHHHHHHHHHHCCC
RAAVVLPDNVLFEAGAGETIRKRLLQDFNLHTILRLPTGIFYAQGVKANVLFFSKGQPTK
CEEEEECCCEEEECCCCHHHHHHHHHHCCCEEEEECCCCCEEECCCEEEEEEEECCCCCC
EIWFYDYRTDIKHTLATNKLERHHLDDFVSCYNNRVETYDAENNPQGRWRKYPVDEILTR
EEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHCC
DKTSLDITWIKQGGEVDNRSLAELMADIKDKSQTISAAVVELEKLLANIEEN
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]