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The map label for this gene is hsdS [H]

Identifier: 150006640

GI number: 150006640

Start: 5127935

End: 5129284

Strand: Reverse

Name: hsdS [H]

Synonym: BVU_4161

Alternate gene names: 150006640

Gene position: 5129284-5127935 (Counterclockwise)

Preceding gene: 150006641

Following gene: 150006639

Centisome position: 99.34

GC content: 36.59

Gene sequence:

>1350_bases
ATGGATACAAAAGCGTTACGTCAAAAGATACTCGACCTTGCGATACATGGAAAACTTGTACCCCAAGATCCCAACGATGA
ACCAGCATCAGTTCTGCTCGAACGAATCAAGGCTGAAAAAGAACGACTGATAAAAGAAGGCAAAATTAAACGGAGTAAGA
AGTCTGCAAAGTCTTCCGATACGCCCCATTATCCCTATTTGTTACCCAATGGGTGGGAATGGTGCAATTTAGAAGATATT
GTGTGCGAATTGAAATACGGAACTTCAGAGAAGTCTTTAAGTGTGGGAAAAATAGCTGTCTTGCGAATGGGGAATATTAC
TAATGTCGGTACAATCGATTATTCTAATTTAGTGTATTCGTCAAATAATGAAGATATTAAGCTGTACTCACTTGAAAAAG
ACGATTTACTGTTCAATCGCACAAACAGTAGTGAATGGGTAGGGAAAACGGCAATCTATAAGAAAGAACAACCAGCCATA
TATGCTGGCTATCTTATTCGCATTAGACCGATATTGATTTTTTCAGACTACCTTAATACTGTAATGAATAGCAGCTATTA
TCGTAATTGGTGCTATAATGTAAAAACGGATGCTGTTAATCAATCTAATATCAATGCTCAAAAGCTTTCGCAATTAATGA
TACCTATTCCACCCTTAAAGGAACAAGAAAGAATTGTTGTAGAGGTGGCTAAGTGGATTTCTTTAATTGATACTATCAAG
AATAGCAAGGAAGATTTACAAACAACTATCAAGCAGGCAAAAAGTAAAATCCTCAATCTTGCTATTCACGGCAAACTCGT
GCCGCAAGATCCGAATGACGAGCCAGCCATTGAACTGTTGAAGCGCATAAATCCCGACTTCACACCTTGTGATAACGGGC
ATTATACACAGTTGCCTGAAGGATGGGCTATTTGTAAAATGAAACAGATTACAAGTATAACGAATGGGAAGTCTCAAAAA
AATGTTGAAACACTTAACGGTATATATCCAATTTATGGTAGTGGCGGTGTTATAGGCAGAGCTAATCAGTATCTTTGCAT
CGCAGGTTCTACAATAATTGGAAGAAAAGGTACAATTAATAATCCTATATTCGTAGAGGAACATTTTTGGAACGTTGATA
CAGCTTTTGGCTTGAAGGCTAATGATGCTATACTTGATAAATATCTGTATTATTTCTGCTTATCATTTGATTTTAGCAAA
TTAGATAAAAGTACGGCTATGCCCAGTTTAACAAAAACATCTATTGGTAATGTTTTAATACCCATACCGCCATATAAAGA
GCAAGAACGAATTGTGGCTAAAATAGATATGGTGCTTGATACGATGAATGAAATATTACGGGCTGTATAA

Upstream 100 bases:

>100_bases
GATTGAAGAGTTTGAAGAAAATGAGGAATTAAAGAAACTGGAAGAACATGAATAGTAATTGTGCAGCCAAAACTGCACAA
TTACAAACAGAAATATAACG

Downstream 100 bases:

>100_bases
ACAATCCGTTTTTGTTCTGCCTTCGGTGGTATAGGTATCTCTTATTTTGAGTATTCAGAAATCCAATATCGTTTATGAGT
GTAACCAGATTGATATGTGA

Product: type I restriction enzyme EcoAI specificity protein

Products: NA

Alternate protein names: S.EcoAI; Type I restriction enzyme EcoAI specificity protein; S protein [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MDTKALRQKILDLAIHGKLVPQDPNDEPASVLLERIKAEKERLIKEGKIKRSKKSAKSSDTPHYPYLLPNGWEWCNLEDI
VCELKYGTSEKSLSVGKIAVLRMGNITNVGTIDYSNLVYSSNNEDIKLYSLEKDDLLFNRTNSSEWVGKTAIYKKEQPAI
YAGYLIRIRPILIFSDYLNTVMNSSYYRNWCYNVKTDAVNQSNINAQKLSQLMIPIPPLKEQERIVVEVAKWISLIDTIK
NSKEDLQTTIKQAKSKILNLAIHGKLVPQDPNDEPAIELLKRINPDFTPCDNGHYTQLPEGWAICKMKQITSITNGKSQK
NVETLNGIYPIYGSGGVIGRANQYLCIAGSTIIGRKGTINNPIFVEEHFWNVDTAFGLKANDAILDKYLYYFCLSFDFSK
LDKSTAMPSLTKTSIGNVLIPIPPYKEQERIVAKIDMVLDTMNEILRAV

Sequences:

>Translated_449_residues
MDTKALRQKILDLAIHGKLVPQDPNDEPASVLLERIKAEKERLIKEGKIKRSKKSAKSSDTPHYPYLLPNGWEWCNLEDI
VCELKYGTSEKSLSVGKIAVLRMGNITNVGTIDYSNLVYSSNNEDIKLYSLEKDDLLFNRTNSSEWVGKTAIYKKEQPAI
YAGYLIRIRPILIFSDYLNTVMNSSYYRNWCYNVKTDAVNQSNINAQKLSQLMIPIPPLKEQERIVVEVAKWISLIDTIK
NSKEDLQTTIKQAKSKILNLAIHGKLVPQDPNDEPAIELLKRINPDFTPCDNGHYTQLPEGWAICKMKQITSITNGKSQK
NVETLNGIYPIYGSGGVIGRANQYLCIAGSTIIGRKGTINNPIFVEEHFWNVDTAFGLKANDAILDKYLYYFCLSFDFSK
LDKSTAMPSLTKTSIGNVLIPIPPYKEQERIVAKIDMVLDTMNEILRAV
>Mature_449_residues
MDTKALRQKILDLAIHGKLVPQDPNDEPASVLLERIKAEKERLIKEGKIKRSKKSAKSSDTPHYPYLLPNGWEWCNLEDI
VCELKYGTSEKSLSVGKIAVLRMGNITNVGTIDYSNLVYSSNNEDIKLYSLEKDDLLFNRTNSSEWVGKTAIYKKEQPAI
YAGYLIRIRPILIFSDYLNTVMNSSYYRNWCYNVKTDAVNQSNINAQKLSQLMIPIPPLKEQERIVVEVAKWISLIDTIK
NSKEDLQTTIKQAKSKILNLAIHGKLVPQDPNDEPAIELLKRINPDFTPCDNGHYTQLPEGWAICKMKQITSITNGKSQK
NVETLNGIYPIYGSGGVIGRANQYLCIAGSTIIGRKGTINNPIFVEEHFWNVDTAFGLKANDAILDKYLYYFCLSFDFSK
LDKSTAMPSLTKTSIGNVLIPIPPYKEQERIVAKIDMVLDTMNEILRAV

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=408, Percent_Identity=23.0392156862745, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 50793; Mature: 50793

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTKALRQKILDLAIHGKLVPQDPNDEPASVLLERIKAEKERLIKEGKIKRSKKSAKSSD
CCHHHHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCC
TPHYPYLLPNGWEWCNLEDIVCELKYGTSEKSLSVGKIAVLRMGNITNVGTIDYSNLVYS
CCCCCEECCCCCCCCCHHHHEEEEECCCCCCCCCCCEEEEEEECCCCCCCEEECCCEEEE
SNNEDIKLYSLEKDDLLFNRTNSSEWVGKTAIYKKEQPAIYAGYLIRIRPILIFSDYLNT
CCCCCEEEEEECCCCEEEECCCCCCCCCCHHEEECCCCCEEEEEEEEEEHHHHHHHHHHH
VMNSSYYRNWCYNVKTDAVNQSNINAQKLSQLMIPIPPLKEQERIVVEVAKWISLIDTIK
HHCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
NSKEDLQTTIKQAKSKILNLAIHGKLVPQDPNDEPAIELLKRINPDFTPCDNGHYTQLPE
CCHHHHHHHHHHHHHHHEEEEEECEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
GWAICKMKQITSITNGKSQKNVETLNGIYPIYGSGGVIGRANQYLCIAGSTIIGRKGTIN
CCHHEEHHHHHHHCCCCCCCCHHHHCCEEEEECCCCEEECCCCEEEEECCEEECCCCCCC
NPIFVEEHFWNVDTAFGLKANDAILDKYLYYFCLSFDFSKLDKSTAMPSLTKTSIGNVLI
CCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHCCCCEEE
PIPPYKEQERIVAKIDMVLDTMNEILRAV
ECCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDTKALRQKILDLAIHGKLVPQDPNDEPASVLLERIKAEKERLIKEGKIKRSKKSAKSSD
CCHHHHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCC
TPHYPYLLPNGWEWCNLEDIVCELKYGTSEKSLSVGKIAVLRMGNITNVGTIDYSNLVYS
CCCCCEECCCCCCCCCHHHHEEEEECCCCCCCCCCCEEEEEEECCCCCCCEEECCCEEEE
SNNEDIKLYSLEKDDLLFNRTNSSEWVGKTAIYKKEQPAIYAGYLIRIRPILIFSDYLNT
CCCCCEEEEEECCCCEEEECCCCCCCCCCHHEEECCCCCEEEEEEEEEEHHHHHHHHHHH
VMNSSYYRNWCYNVKTDAVNQSNINAQKLSQLMIPIPPLKEQERIVVEVAKWISLIDTIK
HHCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
NSKEDLQTTIKQAKSKILNLAIHGKLVPQDPNDEPAIELLKRINPDFTPCDNGHYTQLPE
CCHHHHHHHHHHHHHHHEEEEEECEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
GWAICKMKQITSITNGKSQKNVETLNGIYPIYGSGGVIGRANQYLCIAGSTIIGRKGTIN
CCHHEEHHHHHHHCCCCCCCCHHHHCCEEEEECCCCEEECCCCEEEEECCEEECCCCCCC
NPIFVEEHFWNVDTAFGLKANDAILDKYLYYFCLSFDFSKLDKSTAMPSLTKTSIGNVLI
CCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHCCCCEEE
PIPPYKEQERIVAKIDMVLDTMNEILRAV
ECCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2642743 [H]