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The map label for this gene is hsdS [H]

Identifier: 150006638

GI number: 150006638

Start: 5125281

End: 5126624

Strand: Direct

Name: hsdS [H]

Synonym: BVU_4159

Alternate gene names: 150006638

Gene position: 5125281-5126624 (Clockwise)

Preceding gene: 150006637

Following gene: 150006645

Centisome position: 99.27

GC content: 36.9

Gene sequence:

>1344_bases
ATGTCTAATACATGGTTTAGATTAAGGAGAGGATTTTTCCTCTCCTTTTTTCCTTCCGATACGCCCCATTATGAGAATGT
GCCGTTTGAGCTGCCGAATTCGTGGGTATGGTGTAGATTGGAAGATATAGCTTATGTTGCATCTGGAAGTACACCAGATA
AAACATGCTTTGTAGAGAATGGTGTTCCTTACATTAAGATGTACAATCTCAGGAATCAAAAAATAGATTTTGCCTATCAT
CCACAATATATTACAGAAGAAGTGCATAATGGAAAGCTCCAGCGTTCGCGCACAGAAGTAGGAGACCTTATTATGAATAT
TGTTGGACCACCTCTTGGTAAACTTGCAATTATTCCTACAACATTGCCACAAGCAAATTTTAATCAGGCCGCTGTACTAA
TACGCCCTTACAAATTTAAAGAGGTGTTGGTATCATATCTAAAAGTCTACTTAGAGGAAATGTCAGAAATAAATTCTATT
GCCACACGAGGTTCTGCCGGACAGGTTAATATATCACTGACACAATCTCAAAATATGCGAATACCAATACCGCCTTTAAA
TGAAGTTAGAAGGATAATTGAAGAGGTGTCTAAGTATGATATTTTGATTGATTCGCTCAAACAAAATATTACTGACATTC
AAAATCTTATAGCCTATACCAAATCCAAAATCCTTGATCTTGCCATTCACGGTAAACTAGTACCGCAAGATCCGAATGAC
GAACCAGCCATTGAACTATTGAAGCGCATAAATCCCGACTTCACACCTTGTGATAACGGGCATTATACGTTTGACGTTCC
GAGCGGGTGGATAACGACAAATTTAGGGTCAATATTTAATGTCGTATCAGCTAAAAGAATTTTGAAGTCAGATTGGAAGC
ATAGTGGTGTTCCATTTTATAGAGCACGTGAAATAGCCAAGCTGTCTATTTATGGACTTGTAGATAATGAATTATATATT
TCAGAGGAACATTATAACTCATTAAAGGAGAAATTTCCAGTACCCAAAGCGTCAGATATTATGATTTCTGCCGTTGGTAC
TATTGGCAAATGCTACATTGTAAAAGAGTCTGATAAGTTCTATTACAAGGATGCAAGTGTACTTTGTTTGTGTAATGACT
ATCAAATAAATGCGAAATATATTTACCACATCATGAGAAGCGAATATATGCTAAAACAGATGTATGATAACTCAAAGGGT
ACAACTGTTGATACCATTACGATTGAAAAGGCAAAACAATATATATTGCCCCTACCTCCACTTGCCGAGCAACAGCGCAT
AGTAGCCAAGATTGAAGAGACATTCTCCATTTTTGACGGTATTCAAAATTCCTTAGAAGCGTAA

Upstream 100 bases:

>100_bases
TTGTACCATTAGAATGAAGAAGGCTGATTAGTATGAATGTGAATATGCTGCATATAAATTTTCAACCAAAACAATTATGG
ATTGCCGATGAAAAACTTAA

Downstream 100 bases:

>100_bases
ACAAGAACGCTTCTAAGGACTAATGTAACTTTACCAGTTCACCACTTTATCCACAATCTTCTGCTGTTCGCTGGCAGTGC
GGCGTAAATAGATACGAGTG

Product: type I restriction enzyme EcoR124II specificity protein

Products: NA

Alternate protein names: S.EcoAI; Type I restriction enzyme EcoAI specificity protein; S protein [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSNTWFRLRRGFFLSFFPSDTPHYENVPFELPNSWVWCRLEDIAYVASGSTPDKTCFVENGVPYIKMYNLRNQKIDFAYH
PQYITEEVHNGKLQRSRTEVGDLIMNIVGPPLGKLAIIPTTLPQANFNQAAVLIRPYKFKEVLVSYLKVYLEEMSEINSI
ATRGSAGQVNISLTQSQNMRIPIPPLNEVRRIIEEVSKYDILIDSLKQNITDIQNLIAYTKSKILDLAIHGKLVPQDPND
EPAIELLKRINPDFTPCDNGHYTFDVPSGWITTNLGSIFNVVSAKRILKSDWKHSGVPFYRAREIAKLSIYGLVDNELYI
SEEHYNSLKEKFPVPKASDIMISAVGTIGKCYIVKESDKFYYKDASVLCLCNDYQINAKYIYHIMRSEYMLKQMYDNSKG
TTVDTITIEKAKQYILPLPPLAEQQRIVAKIEETFSIFDGIQNSLEA

Sequences:

>Translated_447_residues
MSNTWFRLRRGFFLSFFPSDTPHYENVPFELPNSWVWCRLEDIAYVASGSTPDKTCFVENGVPYIKMYNLRNQKIDFAYH
PQYITEEVHNGKLQRSRTEVGDLIMNIVGPPLGKLAIIPTTLPQANFNQAAVLIRPYKFKEVLVSYLKVYLEEMSEINSI
ATRGSAGQVNISLTQSQNMRIPIPPLNEVRRIIEEVSKYDILIDSLKQNITDIQNLIAYTKSKILDLAIHGKLVPQDPND
EPAIELLKRINPDFTPCDNGHYTFDVPSGWITTNLGSIFNVVSAKRILKSDWKHSGVPFYRAREIAKLSIYGLVDNELYI
SEEHYNSLKEKFPVPKASDIMISAVGTIGKCYIVKESDKFYYKDASVLCLCNDYQINAKYIYHIMRSEYMLKQMYDNSKG
TTVDTITIEKAKQYILPLPPLAEQQRIVAKIEETFSIFDGIQNSLEA
>Mature_446_residues
SNTWFRLRRGFFLSFFPSDTPHYENVPFELPNSWVWCRLEDIAYVASGSTPDKTCFVENGVPYIKMYNLRNQKIDFAYHP
QYITEEVHNGKLQRSRTEVGDLIMNIVGPPLGKLAIIPTTLPQANFNQAAVLIRPYKFKEVLVSYLKVYLEEMSEINSIA
TRGSAGQVNISLTQSQNMRIPIPPLNEVRRIIEEVSKYDILIDSLKQNITDIQNLIAYTKSKILDLAIHGKLVPQDPNDE
PAIELLKRINPDFTPCDNGHYTFDVPSGWITTNLGSIFNVVSAKRILKSDWKHSGVPFYRAREIAKLSIYGLVDNELYIS
EEHYNSLKEKFPVPKASDIMISAVGTIGKCYIVKESDKFYYKDASVLCLCNDYQINAKYIYHIMRSEYMLKQMYDNSKGT
TVDTITIEKAKQYILPLPPLAEQQRIVAKIEETFSIFDGIQNSLEA

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 51188; Mature: 51057

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTWFRLRRGFFLSFFPSDTPHYENVPFELPNSWVWCRLEDIAYVASGSTPDKTCFVEN
CCCCHHHHHCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCEEEEEC
GVPYIKMYNLRNQKIDFAYHPQYITEEVHNGKLQRSRTEVGDLIMNIVGPPLGKLAIIPT
CCCEEEEEECCCCEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEC
TLPQANFNQAAVLIRPYKFKEVLVSYLKVYLEEMSEINSIATRGSAGQVNISLTQSQNMR
CCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCE
IPIPPLNEVRRIIEEVSKYDILIDSLKQNITDIQNLIAYTKSKILDLAIHGKLVPQDPND
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEECCCCCCC
EPAIELLKRINPDFTPCDNGHYTFDVPSGWITTNLGSIFNVVSAKRILKSDWKHSGVPFY
CHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCHH
RAREIAKLSIYGLVDNELYISEEHYNSLKEKFPVPKASDIMISAVGTIGKCYIVKESDKF
HHHHEEEEEEEEEECCEEEEEHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEECCCCE
YYKDASVLCLCNDYQINAKYIYHIMRSEYMLKQMYDNSKGTTVDTITIEKAKQYILPLPP
EEECCCEEEEECCCEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHCCCCCC
LAEQQRIVAKIEETFSIFDGIQNSLEA
CCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNTWFRLRRGFFLSFFPSDTPHYENVPFELPNSWVWCRLEDIAYVASGSTPDKTCFVEN
CCCHHHHHCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCEEEEEC
GVPYIKMYNLRNQKIDFAYHPQYITEEVHNGKLQRSRTEVGDLIMNIVGPPLGKLAIIPT
CCCEEEEEECCCCEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEC
TLPQANFNQAAVLIRPYKFKEVLVSYLKVYLEEMSEINSIATRGSAGQVNISLTQSQNMR
CCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCE
IPIPPLNEVRRIIEEVSKYDILIDSLKQNITDIQNLIAYTKSKILDLAIHGKLVPQDPND
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEECCCCCCC
EPAIELLKRINPDFTPCDNGHYTFDVPSGWITTNLGSIFNVVSAKRILKSDWKHSGVPFY
CHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCHH
RAREIAKLSIYGLVDNELYISEEHYNSLKEKFPVPKASDIMISAVGTIGKCYIVKESDKF
HHHHEEEEEEEEEECCEEEEEHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEECCCCE
YYKDASVLCLCNDYQINAKYIYHIMRSEYMLKQMYDNSKGTTVDTITIEKAKQYILPLPP
EEECCCEEEEECCCEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHCCCCCC
LAEQQRIVAKIEETFSIFDGIQNSLEA
CCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2642743 [H]