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The map label for this gene is lysS

Identifier: 150006587

GI number: 150006587

Start: 5048782

End: 5050512

Strand: Direct

Name: lysS

Synonym: BVU_4107

Alternate gene names: 150006587

Gene position: 5048782-5050512 (Clockwise)

Preceding gene: 150006586

Following gene: 150006588

Centisome position: 97.78

GC content: 44.37

Gene sequence:

>1731_bases
ATGAACGTATTAGAACTAAGTGAACAGGAGATTATCAGACGAAACAGTCTGAATGAATTGCGCGCTATGGGCATCGAGCC
ATACCCCGCAGCTGAGTATGTAACCAATGCATTTTCTACAGATATAAAAGCTGAGTTCAAGGATGATGAGGAACCTCGCA
AAGTATCGGTAGCCGGACGTATGATGAGCCGCCGTGTGATGGGTAAAGCTTCATTTATCGAATTACAGGATTCAAAAGGC
CGTATCCAGGTGTATATCACCCGTGATGACATCTGCCCGGATGAAAACAAAGAAATGTACAACACTGTGTTCAAACGTCT
GCTGGACCTTGGTGACTTTATCGGTATAGAAGGATTCGTATTCAGAACCCAAATGGGTGAAATCAGTATCCACGCTAAAA
AACTGACCGTACTGTCCAAGTCTATCAAACCGCTTCCTATTGTAAAATACAAAGACGGAGTCGCTTACGACAAGTTTGAA
GACCCTGAACTGCGTTATCGCCAACGTTATGTTGACCTTGCTGTGAATGAAGAGATAAAAGATATCTTCATCAAGCGCAG
TAAAGTATATAGCTCTATGCGCGAATATTTCAACTCAAAAGGTTATATGGAAGTGGAAACCCCGATTCTGCAATCCATTG
CCGGCGGAGCAGCCGCACGTCCATTCATAACTCACCACAACGCACTGGATATGCCGCTGTACATGCGTATTGCCAGTGAG
TTGTACCTGAAAAGACTAATTGTAGGTGGTTTCGAAGGAGTGTATGAAATAGGTAAGAACTTCCGTAACGAAGGAATGGA
CCGCACACACAACCCTGAATTTACCTGCATGGAAATATATGTTGCCTACAAGGACTATAACTGGATGATGGAATTTACAG
AAAAAATGATTGAAAAAATCTGTCTGGATGTAAACGGCACCACTCAAGTAAAAGTAGGCGACAACATCATTGACTTCAAA
GCCCCGTACAAACGTGTCACCATGCTGGACTCTATCAAGGAACACACCGGCTATGATCTGACAGGCATGAACGAAGAACA
GATACGAGAAGTTTGCCAGAAACTCAATATGGAGATTGACGACACAATGGGCAAAGGCAAACTGATCGATGAAATCTTCG
GAGAATTCTGCGAAGGTACTTACATCCAGCCGACTTTCATCACCGACTATCCGAAAGAAATGTCTCCGCTGACAAAGATA
CACCGCAGCAATCCGGATTTGACCGAGCGTTTCGAGCTGATGGTGAACGGCAAGGAACTGTGTAATGCATACTCCGAATT
GAACGACCCGATTGACCAGTTGGAACGTTTCGAAGAACAAATGAAACTGAGCGAAAAAGGAGATGATGAAGCTATGATTA
TTGACAAAGACTTCGTCCGTGCGCTGGAATACGGTATGCCCCCCACTTCGGGTATGGGAATCGGTATGGACCGTCTGACG
ATGCTGATGACCGGACAGTCTACCATTCAGGAAGTATTGTTCTTCCCCCAGATGCGTCCCGAAAAGATTACTCCGAAAGA
CACTCCTGCCAAATTTATGGAACTGGGAATTTCAGAAGACTGGGTTCCTGTCATTCAGAAAGCAGGCTATAACCTGGTGA
GCGATATGAAAGAAGTGAATCCGCAGAAGCTGCATATGGACATTTGCGGAATCAACAAAAAATATAAATTGGAACTAACC
AATCCTACGGTGGACGAAGTTGCCGGATGGATTGCGAAAATTGAGAATTGA

Upstream 100 bases:

>100_bases
CTCAAGAACAGGCTTTATCGAAATAAAAACACGTCTTAATGAGGTACTTTGCAAAATCTTTCGTATTTTTGCCAACAAAT
TGAGTTAATGTTTTAACAGG

Downstream 100 bases:

>100_bases
GAATTTAAAGATTGAGAATTATTCCAATTGTCAATTATCCATCATAAATAATAAGCATGAAATTGCCCGGTAAAATAGCG
ATAATGGGTGGTGGCAGCTG

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MNVLELSEQEIIRRNSLNELRAMGIEPYPAAEYVTNAFSTDIKAEFKDDEEPRKVSVAGRMMSRRVMGKASFIELQDSKG
RIQVYITRDDICPDENKEMYNTVFKRLLDLGDFIGIEGFVFRTQMGEISIHAKKLTVLSKSIKPLPIVKYKDGVAYDKFE
DPELRYRQRYVDLAVNEEIKDIFIKRSKVYSSMREYFNSKGYMEVETPILQSIAGGAAARPFITHHNALDMPLYMRIASE
LYLKRLIVGGFEGVYEIGKNFRNEGMDRTHNPEFTCMEIYVAYKDYNWMMEFTEKMIEKICLDVNGTTQVKVGDNIIDFK
APYKRVTMLDSIKEHTGYDLTGMNEEQIREVCQKLNMEIDDTMGKGKLIDEIFGEFCEGTYIQPTFITDYPKEMSPLTKI
HRSNPDLTERFELMVNGKELCNAYSELNDPIDQLERFEEQMKLSEKGDDEAMIIDKDFVRALEYGMPPTSGMGIGMDRLT
MLMTGQSTIQEVLFFPQMRPEKITPKDTPAKFMELGISEDWVPVIQKAGYNLVSDMKEVNPQKLHMDICGINKKYKLELT
NPTVDEVAGWIAKIEN

Sequences:

>Translated_576_residues
MNVLELSEQEIIRRNSLNELRAMGIEPYPAAEYVTNAFSTDIKAEFKDDEEPRKVSVAGRMMSRRVMGKASFIELQDSKG
RIQVYITRDDICPDENKEMYNTVFKRLLDLGDFIGIEGFVFRTQMGEISIHAKKLTVLSKSIKPLPIVKYKDGVAYDKFE
DPELRYRQRYVDLAVNEEIKDIFIKRSKVYSSMREYFNSKGYMEVETPILQSIAGGAAARPFITHHNALDMPLYMRIASE
LYLKRLIVGGFEGVYEIGKNFRNEGMDRTHNPEFTCMEIYVAYKDYNWMMEFTEKMIEKICLDVNGTTQVKVGDNIIDFK
APYKRVTMLDSIKEHTGYDLTGMNEEQIREVCQKLNMEIDDTMGKGKLIDEIFGEFCEGTYIQPTFITDYPKEMSPLTKI
HRSNPDLTERFELMVNGKELCNAYSELNDPIDQLERFEEQMKLSEKGDDEAMIIDKDFVRALEYGMPPTSGMGIGMDRLT
MLMTGQSTIQEVLFFPQMRPEKITPKDTPAKFMELGISEDWVPVIQKAGYNLVSDMKEVNPQKLHMDICGINKKYKLELT
NPTVDEVAGWIAKIEN
>Mature_576_residues
MNVLELSEQEIIRRNSLNELRAMGIEPYPAAEYVTNAFSTDIKAEFKDDEEPRKVSVAGRMMSRRVMGKASFIELQDSKG
RIQVYITRDDICPDENKEMYNTVFKRLLDLGDFIGIEGFVFRTQMGEISIHAKKLTVLSKSIKPLPIVKYKDGVAYDKFE
DPELRYRQRYVDLAVNEEIKDIFIKRSKVYSSMREYFNSKGYMEVETPILQSIAGGAAARPFITHHNALDMPLYMRIASE
LYLKRLIVGGFEGVYEIGKNFRNEGMDRTHNPEFTCMEIYVAYKDYNWMMEFTEKMIEKICLDVNGTTQVKVGDNIIDFK
APYKRVTMLDSIKEHTGYDLTGMNEEQIREVCQKLNMEIDDTMGKGKLIDEIFGEFCEGTYIQPTFITDYPKEMSPLTKI
HRSNPDLTERFELMVNGKELCNAYSELNDPIDQLERFEEQMKLSEKGDDEAMIIDKDFVRALEYGMPPTSGMGIGMDRLT
MLMTGQSTIQEVLFFPQMRPEKITPKDTPAKFMELGISEDWVPVIQKAGYNLVSDMKEVNPQKLHMDICGINKKYKLELT
NPTVDEVAGWIAKIEN

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI194272210, Length=509, Percent_Identity=41.8467583497053, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI5031815, Length=509, Percent_Identity=41.8467583497053, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI45439306, Length=484, Percent_Identity=23.1404958677686, Blast_Score=99, Evalue=9e-21,
Organism=Escherichia coli, GI1790571, Length=501, Percent_Identity=47.7045908183633, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1789256, Length=511, Percent_Identity=46.9667318982387, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI87082379, Length=322, Percent_Identity=29.1925465838509, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1788173, Length=278, Percent_Identity=24.8201438848921, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=508, Percent_Identity=42.3228346456693, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17535927, Length=508, Percent_Identity=42.3228346456693, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71994340, Length=508, Percent_Identity=42.3228346456693, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI32566633, Length=366, Percent_Identity=22.6775956284153, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=469, Percent_Identity=41.7910447761194, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324256, Length=481, Percent_Identity=31.3929313929314, Blast_Score=193, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6321807, Length=526, Percent_Identity=21.8631178707224, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24640849, Length=511, Percent_Identity=39.9217221135029, Blast_Score=384, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24640851, Length=511, Percent_Identity=39.9217221135029, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24584738, Length=276, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYK_BACV8 (A6L7P5)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001301331.1
- ProteinModelPortal:   A6L7P5
- SMR:   A6L7P5
- STRING:   A6L7P5
- GeneID:   5305066
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_4107
- NMPDR:   fig|435590.6.peg.3819
- eggNOG:   COG1190
- HOGENOM:   HBG631383
- OMA:   GHPQMMS
- ProtClustDB:   PRK00484
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 66462; Mature: 66462

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLELSEQEIIRRNSLNELRAMGIEPYPAAEYVTNAFSTDIKAEFKDDEEPRKVSVAGR
CCCCCCCHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHCCCCEECCCCCCCCHHHHHHHH
MMSRRVMGKASFIELQDSKGRIQVYITRDDICPDENKEMYNTVFKRLLDLGDFIGIEGFV
HHHHHHHCCCEEEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEE
FRTQMGEISIHAKKLTVLSKSIKPLPIVKYKDGVAYDKFEDPELRYRQRYVDLAVNEEIK
EEEECCCEEEEHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHHH
DIFIKRSKVYSSMREYFNSKGYMEVETPILQSIAGGAAARPFITHHNALDMPLYMRIASE
HHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHH
LYLKRLIVGGFEGVYEIGKNFRNEGMDRTHNPEFTCMEIYVAYKDYNWMMEFTEKMIEKI
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHH
CLDVNGTTQVKVGDNIIDFKAPYKRVTMLDSIKEHTGYDLTGMNEEQIREVCQKLNMEID
HCCCCCCEEEEECCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCC
DTMGKGKLIDEIFGEFCEGTYIQPTFITDYPKEMSPLTKIHRSNPDLTERFELMVNGKEL
CCCCCCHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHHCCHHHH
CNAYSELNDPIDQLERFEEQMKLSEKGDDEAMIIDKDFVRALEYGMPPTSGMGIGMDRLT
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCHHHHH
MLMTGQSTIQEVLFFPQMRPEKITPKDTPAKFMELGISEDWVPVIQKAGYNLVSDMKEVN
HHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHCCCHHHHHHHHCC
PQKLHMDICGINKKYKLELTNPTVDEVAGWIAKIEN
HHHEEEEEECCCCEEEEEECCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNVLELSEQEIIRRNSLNELRAMGIEPYPAAEYVTNAFSTDIKAEFKDDEEPRKVSVAGR
CCCCCCCHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHCCCCEECCCCCCCCHHHHHHHH
MMSRRVMGKASFIELQDSKGRIQVYITRDDICPDENKEMYNTVFKRLLDLGDFIGIEGFV
HHHHHHHCCCEEEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEE
FRTQMGEISIHAKKLTVLSKSIKPLPIVKYKDGVAYDKFEDPELRYRQRYVDLAVNEEIK
EEEECCCEEEEHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHHH
DIFIKRSKVYSSMREYFNSKGYMEVETPILQSIAGGAAARPFITHHNALDMPLYMRIASE
HHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHH
LYLKRLIVGGFEGVYEIGKNFRNEGMDRTHNPEFTCMEIYVAYKDYNWMMEFTEKMIEKI
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHH
CLDVNGTTQVKVGDNIIDFKAPYKRVTMLDSIKEHTGYDLTGMNEEQIREVCQKLNMEID
HCCCCCCEEEEECCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCC
DTMGKGKLIDEIFGEFCEGTYIQPTFITDYPKEMSPLTKIHRSNPDLTERFELMVNGKEL
CCCCCCHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHHCCHHHH
CNAYSELNDPIDQLERFEEQMKLSEKGDDEAMIIDKDFVRALEYGMPPTSGMGIGMDRLT
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCHHHHH
MLMTGQSTIQEVLFFPQMRPEKITPKDTPAKFMELGISEDWVPVIQKAGYNLVSDMKEVN
HHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHCCCHHHHHHHHCC
PQKLHMDICGINKKYKLELTNPTVDEVAGWIAKIEN
HHHEEEEEECCCCEEEEEECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA