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The map label for this gene is eptB [H]

Identifier: 150006554

GI number: 150006554

Start: 5010936

End: 5012642

Strand: Reverse

Name: eptB [H]

Synonym: BVU_4074

Alternate gene names: 150006554

Gene position: 5012642-5010936 (Counterclockwise)

Preceding gene: 150006555

Following gene: 150006553

Centisome position: 97.08

GC content: 40.36

Gene sequence:

>1707_bases
ATGAAGCATTTTAAAGAATTTATTCAGTGGTGTTGTGAGCCACAACGGTTGTTCGTTCTGTTCATCGTTGTCTTGGCCAT
TCCCAATGTGGCATTGTTTTTTACAGAACAGCAAATGACACTTTGGGCACGTATTTGTAATGTCATTCTGCCGGTATCGG
TTTATTGGTTGATTATGACATTGGGGCGTAAACCTGGAAAGACTATTTGGATCTTATTTCCGTTTGTTTTCTTTGCGGCA
TTCCAACTGGTATTGCTATACCTGTTCGGGCGTTCTATCATTGCAGTAGATATGTTCCTGAATCTGACAACTACTAATTC
CGGGGAGGCGCTGGAACTGTTGGATAATTTACTGCCGGCTGTAATTGGTGTTTTTGTTGTTTACATTCCTGCTTTGGTCT
TGGGAGGTTTTTCATGGGCCCGGGGTAATCAGTTGGAATATTCTTTTATTCGTTCTCAACGTAAGTATGCCTTGGCAGGC
ATTGTTGCAGGAGCGTTATTGACCGTTATCTGCTATGCGACGCAACGTGATTATCAAGTAAAGATCGAAATGTATCCGGC
TAATGTATGTTATAATCTAGTATTGGCTGCAGAACGTGCGGGCGAAACAGCCGGATATGCGGAAACTTCAAGGGATTTTA
CTTTTAATGCCAGTGCCGCACATGATGAAAACAGCCGGGAAGTTTATGTCTTGGTGATAGGGGAAACGGCCCGTGCCTGT
AATTTCGGACTTTATGGTTATGAAAGGAATACTACTCCATTACTTGATAAAATGGAGGGGGTGGTAACTTTCACGGATGT
GCTTACCCAGTCTAATACTACTCATAAAAGTGTGCCTATGTTGCTTTCGGCAGCTTCGGCTGAGGATTATGACTGTCTTT
ATAGACAGAAAGGAATCATTACCGCTTTCAAGGAAGCAGGATTTCATACTGCCTTTTTTTCGAACCAGCTTCCTAACCAT
TCTTTTATTGATTTTTTGGGGATGGAGGCAGATGATTGGAAGTTTATCAAGAAAGATGCTCCGAAAGGAGCCAATATTTC
GGATGACGAACTATTGTTTCTGGTAGAAAAGGAGCTAAAGGCAGGTCATCAAAAATTATTCATCGTGCTGCATGCCTATG
GATCACATTTTAATTATAAAGAACGTTATCCCGAATCCATGTCTGTCTTTAAACCGGATAATCTGACAGACGCCAAATAT
GAGAATAAAGAGTATTTGATGAATGCATATGATAATACCATCCGTTATACAGATGGTTTCCTGGCTTCTTTGATCACTTT
ATTGCAGAAAACAAACTCTTTTTCGGCTATGCTTTATACTTCGGATCATGGAGAGGATATTTTTGATGATAATCGAAAAT
TATTTTTACATGCATCCCCAGTCCCGTCTTATTATCAACTGCATGTGCCATTTTTAATTTGGTTGTCCAAAACATATAGA
GAAAAGAATGTGGAAGTACATGAAGCTATATTGCAGAACCGTGAAAAACCGGTGGCGGGAAATGCCTCCGTGTTTCATAC
CATGCTGAATTTGGCAGGGGTACAGACACCTTATAGGGCAGATAGCCTATCGGTAGCAAACAGACAATATCTCATTCGTC
CGCGTTATTATCTTAATGACCATAATCTTCCTAAGTCATTGGATAAGATAGGATTAAAAAAGGAAGACATAGAACAGTTC
AGACGGAATAATCTTGTTTTTCCTTGA

Upstream 100 bases:

>100_bases
TATGGAATAGGTTATCGTTGGGAGTTTAAAAAGAATGTAAATGTTCGTCTGGACTATGGATTTGGAAAAAATGGACAGTC
TGGTTTTATCTTTAATATAA

Downstream 100 bases:

>100_bases
TGTGAAAAAGATTTAAAATGGTTGGAGAATTATAATAGAATTACGTATTAATGATTATTTTCGCACAGCTTTGTTATTGT
ATTTATTACGAAAACTATTA

Product: putative sulfatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MKHFKEFIQWCCEPQRLFVLFIVVLAIPNVALFFTEQQMTLWARICNVILPVSVYWLIMTLGRKPGKTIWILFPFVFFAA
FQLVLLYLFGRSIIAVDMFLNLTTTNSGEALELLDNLLPAVIGVFVVYIPALVLGGFSWARGNQLEYSFIRSQRKYALAG
IVAGALLTVICYATQRDYQVKIEMYPANVCYNLVLAAERAGETAGYAETSRDFTFNASAAHDENSREVYVLVIGETARAC
NFGLYGYERNTTPLLDKMEGVVTFTDVLTQSNTTHKSVPMLLSAASAEDYDCLYRQKGIITAFKEAGFHTAFFSNQLPNH
SFIDFLGMEADDWKFIKKDAPKGANISDDELLFLVEKELKAGHQKLFIVLHAYGSHFNYKERYPESMSVFKPDNLTDAKY
ENKEYLMNAYDNTIRYTDGFLASLITLLQKTNSFSAMLYTSDHGEDIFDDNRKLFLHASPVPSYYQLHVPFLIWLSKTYR
EKNVEVHEAILQNREKPVAGNASVFHTMLNLAGVQTPYRADSLSVANRQYLIRPRYYLNDHNLPKSLDKIGLKKEDIEQF
RRNNLVFP

Sequences:

>Translated_568_residues
MKHFKEFIQWCCEPQRLFVLFIVVLAIPNVALFFTEQQMTLWARICNVILPVSVYWLIMTLGRKPGKTIWILFPFVFFAA
FQLVLLYLFGRSIIAVDMFLNLTTTNSGEALELLDNLLPAVIGVFVVYIPALVLGGFSWARGNQLEYSFIRSQRKYALAG
IVAGALLTVICYATQRDYQVKIEMYPANVCYNLVLAAERAGETAGYAETSRDFTFNASAAHDENSREVYVLVIGETARAC
NFGLYGYERNTTPLLDKMEGVVTFTDVLTQSNTTHKSVPMLLSAASAEDYDCLYRQKGIITAFKEAGFHTAFFSNQLPNH
SFIDFLGMEADDWKFIKKDAPKGANISDDELLFLVEKELKAGHQKLFIVLHAYGSHFNYKERYPESMSVFKPDNLTDAKY
ENKEYLMNAYDNTIRYTDGFLASLITLLQKTNSFSAMLYTSDHGEDIFDDNRKLFLHASPVPSYYQLHVPFLIWLSKTYR
EKNVEVHEAILQNREKPVAGNASVFHTMLNLAGVQTPYRADSLSVANRQYLIRPRYYLNDHNLPKSLDKIGLKKEDIEQF
RRNNLVFP
>Mature_568_residues
MKHFKEFIQWCCEPQRLFVLFIVVLAIPNVALFFTEQQMTLWARICNVILPVSVYWLIMTLGRKPGKTIWILFPFVFFAA
FQLVLLYLFGRSIIAVDMFLNLTTTNSGEALELLDNLLPAVIGVFVVYIPALVLGGFSWARGNQLEYSFIRSQRKYALAG
IVAGALLTVICYATQRDYQVKIEMYPANVCYNLVLAAERAGETAGYAETSRDFTFNASAAHDENSREVYVLVIGETARAC
NFGLYGYERNTTPLLDKMEGVVTFTDVLTQSNTTHKSVPMLLSAASAEDYDCLYRQKGIITAFKEAGFHTAFFSNQLPNH
SFIDFLGMEADDWKFIKKDAPKGANISDDELLFLVEKELKAGHQKLFIVLHAYGSHFNYKERYPESMSVFKPDNLTDAKY
ENKEYLMNAYDNTIRYTDGFLASLITLLQKTNSFSAMLYTSDHGEDIFDDNRKLFLHASPVPSYYQLHVPFLIWLSKTYR
EKNVEVHEAILQNREKPVAGNASVFHTMLNLAGVQTPYRADSLSVANRQYLIRPRYYLNDHNLPKSLDKIGLKKEDIEQF
RRNNLVFP

Specific function: Catalyzes the addition of a phosphoethanolamine (pEtN) moiety to the outer 3-deoxy-D-manno-octulosonic acid (KDO) residue of a KDO(2)-lipid A. Phosphatidylethanolamines with one unsaturated acyl group functions as pEtN donors and the reaction releases dia

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082286, Length=365, Percent_Identity=28.4931506849315, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87082372, Length=308, Percent_Identity=30.5194805194805, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI87082223, Length=507, Percent_Identity=25.6410256410256, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1790392, Length=332, Percent_Identity=28.0120481927711, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787035, Length=255, Percent_Identity=29.0196078431373, Blast_Score=76, Evalue=7e-15,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 64926; Mature: 64926

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHFKEFIQWCCEPQRLFVLFIVVLAIPNVALFFTEQQMTLWARICNVILPVSVYWLIMT
CHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
LGRKPGKTIWILFPFVFFAAFQLVLLYLFGRSIIAVDMFLNLTTTNSGEALELLDNLLPA
HCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCHHEEEEEEEEEEECCCCCHHHHHHHHHHH
VIGVFVVYIPALVLGGFSWARGNQLEYSFIRSQRKYALAGIVAGALLTVICYATQRDYQV
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
KIEMYPANVCYNLVLAAERAGETAGYAETSRDFTFNASAAHDENSREVYVLVIGETARAC
EEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEEEECCCCCHH
NFGLYGYERNTTPLLDKMEGVVTFTDVLTQSNTTHKSVPMLLSAASAEDYDCLYRQKGII
CCCCCCCCCCCCHHHHHHCCCEEHHHHHHCCCCCCHHHHHHHEECCCCCHHHHHHCCCCH
TAFKEAGFHTAFFSNQLPNHSFIDFLGMEADDWKFIKKDAPKGANISDDELLFLVEKELK
HHHHHCCCEEEHHHCCCCCCCHHHHHCCCCCCCHHHHCCCCCCCCCCCCCEEEEEEHHHC
AGHQKLFIVLHAYGSHFNYKERYPESMSVFKPDNLTDAKYENKEYLMNAYDNTIRYTDGF
CCCEEEEEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECHHH
LASLITLLQKTNSFSAMLYTSDHGEDIFDDNRKLFLHASPVPSYYQLHVPFLIWLSKTYR
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEECHHHHHHHHHHH
EKNVEVHEAILQNREKPVAGNASVFHTMLNLAGVQTPYRADSLSVANRQYLIRPRYYLND
HCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCEEECC
HNLPKSLDKIGLKKEDIEQFRRNNLVFP
CCCCHHHHHHCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKHFKEFIQWCCEPQRLFVLFIVVLAIPNVALFFTEQQMTLWARICNVILPVSVYWLIMT
CHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
LGRKPGKTIWILFPFVFFAAFQLVLLYLFGRSIIAVDMFLNLTTTNSGEALELLDNLLPA
HCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCHHEEEEEEEEEEECCCCCHHHHHHHHHHH
VIGVFVVYIPALVLGGFSWARGNQLEYSFIRSQRKYALAGIVAGALLTVICYATQRDYQV
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
KIEMYPANVCYNLVLAAERAGETAGYAETSRDFTFNASAAHDENSREVYVLVIGETARAC
EEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEEEECCCCCHH
NFGLYGYERNTTPLLDKMEGVVTFTDVLTQSNTTHKSVPMLLSAASAEDYDCLYRQKGII
CCCCCCCCCCCCHHHHHHCCCEEHHHHHHCCCCCCHHHHHHHEECCCCCHHHHHHCCCCH
TAFKEAGFHTAFFSNQLPNHSFIDFLGMEADDWKFIKKDAPKGANISDDELLFLVEKELK
HHHHHCCCEEEHHHCCCCCCCHHHHHCCCCCCCHHHHCCCCCCCCCCCCCEEEEEEHHHC
AGHQKLFIVLHAYGSHFNYKERYPESMSVFKPDNLTDAKYENKEYLMNAYDNTIRYTDGF
CCCEEEEEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECHHH
LASLITLLQKTNSFSAMLYTSDHGEDIFDDNRKLFLHASPVPSYYQLHVPFLIWLSKTYR
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEECHHHHHHHHHHH
EKNVEVHEAILQNREKPVAGNASVFHTMLNLAGVQTPYRADSLSVANRQYLIRPRYYLND
HCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCEEECC
HNLPKSLDKIGLKKEDIEQFRRNNLVFP
CCCCHHHHHHCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 9298646 [H]