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The map label for this gene is carA [H]

Identifier: 150006479

GI number: 150006479

Start: 4922128

End: 4923204

Strand: Reverse

Name: carA [H]

Synonym: BVU_3998

Alternate gene names: 150006479

Gene position: 4923204-4922128 (Counterclockwise)

Preceding gene: 150006480

Following gene: 150006478

Centisome position: 95.35

GC content: 46.89

Gene sequence:

>1077_bases
ATGAGAAATGTAACATTGATCCTCGACGACGGGAGCCGTTTCCCGGGCAAGTCGTTCGGTTATGAGAAACCGGTAGCCGG
CGAAGTGGTGTTTAATACCGCTATGACCGGTTATCCTGAGAGTTTGACAGACCCTTCATATGCGGGGCAGCTGATGACCT
TAACTTATCCGTTGGTCGGTAACTACGGGGTACCTCCCTTTACTATCGAACCCAATGGCTTGGCTACTTTTATGGAAAGT
GAACGTATCCATGCCGAAGCGATTATTGTAAGTGATTACAGTGAAGAATACAGTCACTGGAATGCGAAAGAAAGTCTGGC
CGACTGGCTGAAGCGTGAGCAAGTGCCGGGCATTACAGGCATCGATACCCGTGAACTGACCAAAGTTCTTCGCGAACATG
GGGTAATGATGGGGAAAATTGTATTTGATGATGAACCCGGCAATGTGCCCGAAGCAATGTATGCAGGTATCAATTATGTA
GATAAAGTATCGTGTAAGGAAGTGATTCGTTACAATGAAGGTGCAGGTAAGAAAAAAGTGGTGTTGGTGGATTGTGGTGT
AAAGACAAATATTATCCGTTGCTTACTGAAACGGGATGTGGAAGTGATCCGTGTTCCTTGGAACCATGACTTTACAGGGA
TGGAGTATGACGGATTGTTTATTTCGAACGGTCCGGGTGATCCGGATACTTGCGATGAGGCGGTACAGCATATCCGCAAG
GCGATGAGCCTGAGCGACAAGCCTATCTTCGGCATTTGTATGGGGAATCAGTTGCTTTCCAAAGCAGGAGGTGCCACCAT
CTATAAATTGAAATACGGTCATCGCAGCCATAACCAACCGGTGCGCATGATAGGAACGGAACGTTGTTTCATTACCAGTC
AGAACCACGGTTATGCGGTAGATAATAATACATTGGGAACCGATTGGGAACCGCTGTTCATCAATATGAATGACGGTTCC
AACGAGGGTATCAGGCATAAAACCAAACCTTGGTTCTCGGCACAGTTCCATCCGGAAGCTGCATCGGGACCTACGGATAC
CGAGTTCCTGTTTGATGAGTTTGTGAAGTTGTTATAA

Upstream 100 bases:

>100_bases
TATCCTACTCCGGGTGGAGTGAAGCTGGTGAACCAGGCGTTTATAGACTATATAGAAAAAGTTTATCAATTCTAAAGAAC
AACCATTCAAGATAGATTAG

Downstream 100 bases:

>100_bases
ACCGTGTATTAAAAAGAACAAGTATGAAAGACGAAATAAAGAAAGTATTGTTATTAGGTTCCGGTGCCTTGAAGATTGGC
GAGGCCGGAGAATTTGACTA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MRNVTLILDDGSRFPGKSFGYEKPVAGEVVFNTAMTGYPESLTDPSYAGQLMTLTYPLVGNYGVPPFTIEPNGLATFMES
ERIHAEAIIVSDYSEEYSHWNAKESLADWLKREQVPGITGIDTRELTKVLREHGVMMGKIVFDDEPGNVPEAMYAGINYV
DKVSCKEVIRYNEGAGKKKVVLVDCGVKTNIIRCLLKRDVEVIRVPWNHDFTGMEYDGLFISNGPGDPDTCDEAVQHIRK
AMSLSDKPIFGICMGNQLLSKAGGATIYKLKYGHRSHNQPVRMIGTERCFITSQNHGYAVDNNTLGTDWEPLFINMNDGS
NEGIRHKTKPWFSAQFHPEAASGPTDTEFLFDEFVKLL

Sequences:

>Translated_358_residues
MRNVTLILDDGSRFPGKSFGYEKPVAGEVVFNTAMTGYPESLTDPSYAGQLMTLTYPLVGNYGVPPFTIEPNGLATFMES
ERIHAEAIIVSDYSEEYSHWNAKESLADWLKREQVPGITGIDTRELTKVLREHGVMMGKIVFDDEPGNVPEAMYAGINYV
DKVSCKEVIRYNEGAGKKKVVLVDCGVKTNIIRCLLKRDVEVIRVPWNHDFTGMEYDGLFISNGPGDPDTCDEAVQHIRK
AMSLSDKPIFGICMGNQLLSKAGGATIYKLKYGHRSHNQPVRMIGTERCFITSQNHGYAVDNNTLGTDWEPLFINMNDGS
NEGIRHKTKPWFSAQFHPEAASGPTDTEFLFDEFVKLL
>Mature_358_residues
MRNVTLILDDGSRFPGKSFGYEKPVAGEVVFNTAMTGYPESLTDPSYAGQLMTLTYPLVGNYGVPPFTIEPNGLATFMES
ERIHAEAIIVSDYSEEYSHWNAKESLADWLKREQVPGITGIDTRELTKVLREHGVMMGKIVFDDEPGNVPEAMYAGINYV
DKVSCKEVIRYNEGAGKKKVVLVDCGVKTNIIRCLLKRDVEVIRVPWNHDFTGMEYDGLFISNGPGDPDTCDEAVQHIRK
AMSLSDKPIFGICMGNQLLSKAGGATIYKLKYGHRSHNQPVRMIGTERCFITSQNHGYAVDNNTLGTDWEPLFINMNDGS
NEGIRHKTKPWFSAQFHPEAASGPTDTEFLFDEFVKLL

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=354, Percent_Identity=49.4350282485876, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI169790915, Length=355, Percent_Identity=49.0140845070423, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI21361331, Length=355, Percent_Identity=49.0140845070423, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI1786215, Length=383, Percent_Identity=38.9033942558747, Blast_Score=262, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI193204318, Length=355, Percent_Identity=54.0845070422535, Blast_Score=376, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6322331, Length=386, Percent_Identity=47.6683937823834, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324878, Length=359, Percent_Identity=49.025069637883, Blast_Score=349, Evalue=4e-97,
Organism=Drosophila melanogaster, GI45555749, Length=365, Percent_Identity=49.041095890411, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24642586, Length=365, Percent_Identity=49.041095890411, Blast_Score=359, Evalue=2e-99,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39979; Mature: 39979

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNVTLILDDGSRFPGKSFGYEKPVAGEVVFNTAMTGYPESLTDPSYAGQLMTLTYPLVG
CCCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHCCEEEEEEEEECC
NYGVPPFTIEPNGLATFMESERIHAEAIIVSDYSEEYSHWNAKESLADWLKREQVPGITG
CCCCCCEEECCCCCEEHHHCCCEEEEEEEEECCHHHHHCCCHHHHHHHHHHHHCCCCCCC
IDTRELTKVLREHGVMMGKIVFDDEPGNVPEAMYAGINYVDKVSCKEVIRYNEGAGKKKV
CCHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCEEE
VLVDCGVKTNIIRCLLKRDVEVIRVPWNHDFTGMEYDGLFISNGPGDPDTCDEAVQHIRK
EEEECCCHHHHHHHHHHCCCEEEEECCCCCCCCEEECCEEEECCCCCCHHHHHHHHHHHH
AMSLSDKPIFGICMGNQLLSKAGGATIYKLKYGHRSHNQPVRMIGTERCFITSQNHGYAV
HHCCCCCCEEEEEECHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCEEEEEECCCCEEE
DNNTLGTDWEPLFINMNDGSNEGIRHKTKPWFSAQFHPEAASGPTDTEFLFDEFVKLL
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MRNVTLILDDGSRFPGKSFGYEKPVAGEVVFNTAMTGYPESLTDPSYAGQLMTLTYPLVG
CCCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHCCEEEEEEEEECC
NYGVPPFTIEPNGLATFMESERIHAEAIIVSDYSEEYSHWNAKESLADWLKREQVPGITG
CCCCCCEEECCCCCEEHHHCCCEEEEEEEEECCHHHHHCCCHHHHHHHHHHHHCCCCCCC
IDTRELTKVLREHGVMMGKIVFDDEPGNVPEAMYAGINYVDKVSCKEVIRYNEGAGKKKV
CCHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCEEE
VLVDCGVKTNIIRCLLKRDVEVIRVPWNHDFTGMEYDGLFISNGPGDPDTCDEAVQHIRK
EEEECCCHHHHHHHHHHCCCEEEEECCCCCCCCEEECCEEEECCCCCCHHHHHHHHHHHH
AMSLSDKPIFGICMGNQLLSKAGGATIYKLKYGHRSHNQPVRMIGTERCFITSQNHGYAV
HHCCCCCCEEEEEECHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCEEEEEECCCCEEE
DNNTLGTDWEPLFINMNDGSNEGIRHKTKPWFSAQFHPEAASGPTDTEFLFDEFVKLL
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA