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The map label for this gene is thiDE [H]

Identifier: 150006469

GI number: 150006469

Start: 4907484

End: 4908308

Strand: Direct

Name: thiDE [H]

Synonym: BVU_3988

Alternate gene names: 150006469

Gene position: 4907484-4908308 (Clockwise)

Preceding gene: 150006466

Following gene: 150006470

Centisome position: 95.05

GC content: 48.36

Gene sequence:

>825_bases
ATGAAGAAATATCCTTGTGTATTAAGTATAGCAGGATCCGATTGCAGCGGAGGAGCCGGCATACAGGCTGACCTGAAGAC
CATCTCCGCCTTGGGTGGTTATGCTGCGACAGCCATTACTGCAATCACCGTACAGAACACTTTGGGAGTACGTGCCATAC
ATCCCGTACCACCGGTATATGTACGTGGGCAGATAGAAGCCGTAATGGAAGATATCCGTCCGGATGCTGTTAAAATAGGT
ATGATTAATGATGTGGAAATAGTCGAGGCCATAGCCTCCTGCCTTCGTACATACCGACCCCGTTTCGTTGTGTTCGATCC
GGTCATGGTATCGACTAGCGGACACCGGCTGATAGAAGAGGACGCCATCAGTGCACTGACCCGGGAACTGATGCCTCTGG
CCAGTCTGATTACGCCCAATCTGAGTGAGGCGGAAGTATTGACGGAACACTGCATAAGCAATGTGGACACAATGAAAGCT
GCCGCATCGGAATTATTAAAATTCGGTTGCGGATCCGTTTTACTGAAAGGAGGACATTTGGAGGGCGACAAGATGTGCGA
TGTATTACAAACAGCTGGAGAAGACAAGCCTCATCTCTTTATCTCAGATAAGATAGAAAGCAAAAACACACACGGAACAG
GATGTACTCTTTCTTCCGCCATTGCTACTTTTCTGGCACTGGGATATGATATGCCGCAGGCCGTAGAACATGCAAAAGCA
TATGTCACCAAAGGAATCCACGCCGGGAAAGACGTGCATATAGGCGAAGGACACGGCCCGCTGAATCACTTTTACGCACC
TGTTCCGATGAAAACAATGAAATAA

Upstream 100 bases:

>100_bases
TTTATGTTTAGCAAAAATAATTGATTCCCTTCACTTGTGCAAACATTTAAAACAAGATTATTCGTTATCTTTGCAAATAC
TTTGTCAAAATGAGATTTAT

Downstream 100 bases:

>100_bases
TTTGTCAATGTGCTAATTACCATGCGGTACATAATGCAGCATATTAGCAAATTGCCTCATTATTATTCCGTAGTTCCGAA
AGAAATAATTAACTTTGCAA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MKKYPCVLSIAGSDCSGGAGIQADLKTISALGGYAATAITAITVQNTLGVRAIHPVPPVYVRGQIEAVMEDIRPDAVKIG
MINDVEIVEAIASCLRTYRPRFVVFDPVMVSTSGHRLIEEDAISALTRELMPLASLITPNLSEAEVLTEHCISNVDTMKA
AASELLKFGCGSVLLKGGHLEGDKMCDVLQTAGEDKPHLFISDKIESKNTHGTGCTLSSAIATFLALGYDMPQAVEHAKA
YVTKGIHAGKDVHIGEGHGPLNHFYAPVPMKTMK

Sequences:

>Translated_274_residues
MKKYPCVLSIAGSDCSGGAGIQADLKTISALGGYAATAITAITVQNTLGVRAIHPVPPVYVRGQIEAVMEDIRPDAVKIG
MINDVEIVEAIASCLRTYRPRFVVFDPVMVSTSGHRLIEEDAISALTRELMPLASLITPNLSEAEVLTEHCISNVDTMKA
AASELLKFGCGSVLLKGGHLEGDKMCDVLQTAGEDKPHLFISDKIESKNTHGTGCTLSSAIATFLALGYDMPQAVEHAKA
YVTKGIHAGKDVHIGEGHGPLNHFYAPVPMKTMK
>Mature_274_residues
MKKYPCVLSIAGSDCSGGAGIQADLKTISALGGYAATAITAITVQNTLGVRAIHPVPPVYVRGQIEAVMEDIRPDAVKIG
MINDVEIVEAIASCLRTYRPRFVVFDPVMVSTSGHRLIEEDAISALTRELMPLASLITPNLSEAEVLTEHCISNVDTMKA
AASELLKFGCGSVLLKGGHLEGDKMCDVLQTAGEDKPHLFISDKIESKNTHGTGCTLSSAIATFLALGYDMPQAVEHAKA
YVTKGIHAGKDVHIGEGHGPLNHFYAPVPMKTMK

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=262, Percent_Identity=43.5114503816794, Blast_Score=215, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6325378, Length=284, Percent_Identity=35.2112676056338, Blast_Score=157, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324997, Length=285, Percent_Identity=38.9473684210526, Blast_Score=155, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6324517, Length=285, Percent_Identity=38.9473684210526, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 29139; Mature: 29139

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYPCVLSIAGSDCSGGAGIQADLKTISALGGYAATAITAITVQNTLGVRAIHPVPPVY
CCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHEEEECCCCEEEECCCCCEE
VRGQIEAVMEDIRPDAVKIGMINDVEIVEAIASCLRTYRPRFVVFDPVMVSTSGHRLIEE
ECHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCEEEECCCCHHHHH
DAISALTRELMPLASLITPNLSEAEVLTEHCISNVDTMKAAASELLKFGCGSVLLKGGHL
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCC
EGDKMCDVLQTAGEDKPHLFISDKIESKNTHGTGCTLSSAIATFLALGYDMPQAVEHAKA
CHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
YVTKGIHAGKDVHIGEGHGPLNHFYAPVPMKTMK
HHHHCCCCCCCEEECCCCCCHHHCCCCCCHHCCC
>Mature Secondary Structure
MKKYPCVLSIAGSDCSGGAGIQADLKTISALGGYAATAITAITVQNTLGVRAIHPVPPVY
CCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHEEEECCCCEEEECCCCCEE
VRGQIEAVMEDIRPDAVKIGMINDVEIVEAIASCLRTYRPRFVVFDPVMVSTSGHRLIEE
ECHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCEEEECCCCHHHHH
DAISALTRELMPLASLITPNLSEAEVLTEHCISNVDTMKAAASELLKFGCGSVLLKGGHL
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCC
EGDKMCDVLQTAGEDKPHLFISDKIESKNTHGTGCTLSSAIATFLALGYDMPQAVEHAKA
CHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
YVTKGIHAGKDVHIGEGHGPLNHFYAPVPMKTMK
HHHHCCCCCCCEEECCCCCCHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA