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The map label for this gene is pleD [H]

Identifier: 150006467

GI number: 150006467

Start: 4905276

End: 4906415

Strand: Reverse

Name: pleD [H]

Synonym: BVU_3986

Alternate gene names: 150006467

Gene position: 4906415-4905276 (Counterclockwise)

Preceding gene: 150006468

Following gene: 150006464

Centisome position: 95.03

GC content: 40.53

Gene sequence:

>1140_bases
ATGACACCGGATGTAAATGTTGTAATAAGTCATTCTGAGTACACTGTTTTGGTTGTGGATGATGTGGTTTCTAATGTCTT
GTTGTTAAAAGTGTTGTTAACAAACGAGAAGTTTAAGGTGATAACTGCCGGTAATGGAAAGGAGGCATTATCCCAGGCGG
TCAATGCCCGTCCCGATTTGATTTTGTTGGATGTCATGATGCCCGAGATGAATGGTTTTGAAGTTGCCGAACGATTGAAA
GCCGATCCGGAAACACAACATATTCCTATTATATTTCTGACAGCCTTGAATACCACTGCCGATATAGTGAAGGGTTTCAA
GGTAGGTGCGAATGATTTTATATCCAAACCTTTTAATAAGGAAGAGCTTGTGATCCGTGTTACCCATCAGATATCCCTGA
TTGCCGCCAAACGGATCATCATGAAGCAAACGGAAGAATTGCGTAAGATTATTATGGGGCGCGACAAGTTATATTCTGTT
ATCGCACATGACCTGCGTTCACCCATGGGATCCATTAAAATGGTGCTCAATATGTTGATACTCAGTCTTCCGTCCGAACA
GATCGGTAAGGAAATGTTTGATATGCTGAGCATGGCCAATCAAAGTACGGAGGATGTTTTCTCTTTGCTGGATAATTTAT
TGAAATGGACTAAAAGCCAGATAGGCAAGCTGAATGTAGTTTATCAGGACTTTGATATTGTGGGGAATATTGCTTCGGTT
ATCGAAATTTTCAATCTGGTAGCCGGTATGAAGAATATAAAGGTGAACTTCCCGGTGCATGGAAAGATTGAGGTACATGC
CGATATGGATATGATGAAAACCATCTTGCGTAATCTTCTCAGCAATGCAATTAAGTTCAGTTATAATGATTCCGAAATTC
TGGTTAATGCCGAAATAGAGGGTGATAAAGTCATAGTCAGTGTAAAAGACACCGGAAAAGGCATGAGTGAGGAAGACCAA
AAGAAACTGTTGAACACGGAAACTCATTTCAGCAAATACGGAACGAATAATGAGGAAGGTTCCGGATTAGGCTTGTTATT
ATGTCAGGATTTTGCTGTTAAGAATGGTGGGCGTTTATGGTTTGAGTCTGAAGAGGGAAAAGGTTCCACTTTCTTCTTTT
CGGTAGCTTTGAAGAAGTGA

Upstream 100 bases:

>100_bases
AAAAGAAAAGCTGGATTGGGAGCCTCACATTCATCTGGAAGAGGGGCTGATGAAAGTGATTGATTATTTTAAAAGTGTTC
TGGCTAAATAATATATACCT

Downstream 100 bases:

>100_bases
TTTATCAAAGCCCCACCTCCAGATATACCGGGTTATGGTCACTCATATCATAAGGAATCACCTTATACTTTATACCTTCC
AATAAGGTGGAATGGAACAA

Product: two-component system sensor histidine kinase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MTPDVNVVISHSEYTVLVVDDVVSNVLLLKVLLTNEKFKVITAGNGKEALSQAVNARPDLILLDVMMPEMNGFEVAERLK
ADPETQHIPIIFLTALNTTADIVKGFKVGANDFISKPFNKEELVIRVTHQISLIAAKRIIMKQTEELRKIIMGRDKLYSV
IAHDLRSPMGSIKMVLNMLILSLPSEQIGKEMFDMLSMANQSTEDVFSLLDNLLKWTKSQIGKLNVVYQDFDIVGNIASV
IEIFNLVAGMKNIKVNFPVHGKIEVHADMDMMKTILRNLLSNAIKFSYNDSEILVNAEIEGDKVIVSVKDTGKGMSEEDQ
KKLLNTETHFSKYGTNNEEGSGLGLLLCQDFAVKNGGRLWFESEEGKGSTFFFSVALKK

Sequences:

>Translated_379_residues
MTPDVNVVISHSEYTVLVVDDVVSNVLLLKVLLTNEKFKVITAGNGKEALSQAVNARPDLILLDVMMPEMNGFEVAERLK
ADPETQHIPIIFLTALNTTADIVKGFKVGANDFISKPFNKEELVIRVTHQISLIAAKRIIMKQTEELRKIIMGRDKLYSV
IAHDLRSPMGSIKMVLNMLILSLPSEQIGKEMFDMLSMANQSTEDVFSLLDNLLKWTKSQIGKLNVVYQDFDIVGNIASV
IEIFNLVAGMKNIKVNFPVHGKIEVHADMDMMKTILRNLLSNAIKFSYNDSEILVNAEIEGDKVIVSVKDTGKGMSEEDQ
KKLLNTETHFSKYGTNNEEGSGLGLLLCQDFAVKNGGRLWFESEEGKGSTFFFSVALKK
>Mature_378_residues
TPDVNVVISHSEYTVLVVDDVVSNVLLLKVLLTNEKFKVITAGNGKEALSQAVNARPDLILLDVMMPEMNGFEVAERLKA
DPETQHIPIIFLTALNTTADIVKGFKVGANDFISKPFNKEELVIRVTHQISLIAAKRIIMKQTEELRKIIMGRDKLYSVI
AHDLRSPMGSIKMVLNMLILSLPSEQIGKEMFDMLSMANQSTEDVFSLLDNLLKWTKSQIGKLNVVYQDFDIVGNIASVI
EIFNLVAGMKNIKVNFPVHGKIEVHADMDMMKTILRNLLSNAIKFSYNDSEILVNAEIEGDKVIVSVKDTGKGMSEEDQK
KLLNTETHFSKYGTNNEEGSGLGLLLCQDFAVKNGGRLWFESEEGKGSTFFFSVALKK

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788713, Length=238, Percent_Identity=25.6302521008403, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=28.0172413793103, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789149, Length=242, Percent_Identity=25.2066115702479, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786599, Length=131, Percent_Identity=35.8778625954198, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786912, Length=275, Percent_Identity=26.5454545454545, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789809, Length=114, Percent_Identity=34.2105263157895, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI2367329, Length=112, Percent_Identity=45.5357142857143, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1786784, Length=95, Percent_Identity=40, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790437, Length=112, Percent_Identity=34.8214285714286, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790860, Length=113, Percent_Identity=34.5132743362832, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082012, Length=99, Percent_Identity=34.3434343434343, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788550, Length=125, Percent_Identity=31.2, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI145693157, Length=224, Percent_Identity=24.1071428571429, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI48994928, Length=227, Percent_Identity=25.5506607929515, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787894, Length=241, Percent_Identity=22.4066390041494, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786600, Length=276, Percent_Identity=25, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790863, Length=117, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788393, Length=259, Percent_Identity=22.3938223938224, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788394, Length=131, Percent_Identity=29.0076335877863, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786911, Length=112, Percent_Identity=31.25, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786783, Length=260, Percent_Identity=20.7692307692308, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082128, Length=236, Percent_Identity=22.0338983050847, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42246; Mature: 42115

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPDVNVVISHSEYTVLVVDDVVSNVLLLKVLLTNEKFKVITAGNGKEALSQAVNARPDL
CCCCCEEEEECCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCE
ILLDVMMPEMNGFEVAERLKADPETQHIPIIFLTALNTTADIVKGFKVGANDFISKPFNK
EEEEEECCCCCCHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCHHHHCCCCCC
EELVIRVTHQISLIAAKRIIMKQTEELRKIIMGRDKLYSVIAHDLRSPMGSIKMVLNMLI
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHH
LSLPSEQIGKEMFDMLSMANQSTEDVFSLLDNLLKWTKSQIGKLNVVYQDFDIVGNIASV
HHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHHHHH
IEIFNLVAGMKNIKVNFPVHGKIEVHADMDMMKTILRNLLSNAIKFSYNDSEILVNAEIE
HHHHHHHCCCHHCEEECCCCCEEEEECCHHHHHHHHHHHHHHHHEEEECCCEEEEEEEEC
GDKVIVSVKDTGKGMSEEDQKKLLNTETHFSKYGTNNEEGSGLGLLLCQDFAVKNGGRLW
CCEEEEEEECCCCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEEEEHHHCCCCCEEE
FESEEGKGSTFFFSVALKK
EECCCCCCCEEEEEEEECC
>Mature Secondary Structure 
TPDVNVVISHSEYTVLVVDDVVSNVLLLKVLLTNEKFKVITAGNGKEALSQAVNARPDL
CCCCEEEEECCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCE
ILLDVMMPEMNGFEVAERLKADPETQHIPIIFLTALNTTADIVKGFKVGANDFISKPFNK
EEEEEECCCCCCHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCHHHHCCCCCC
EELVIRVTHQISLIAAKRIIMKQTEELRKIIMGRDKLYSVIAHDLRSPMGSIKMVLNMLI
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHH
LSLPSEQIGKEMFDMLSMANQSTEDVFSLLDNLLKWTKSQIGKLNVVYQDFDIVGNIASV
HHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHHHHH
IEIFNLVAGMKNIKVNFPVHGKIEVHADMDMMKTILRNLLSNAIKFSYNDSEILVNAEIE
HHHHHHHCCCHHCEEECCCCCEEEEECCHHHHHHHHHHHHHHHHEEEECCCEEEEEEEEC
GDKVIVSVKDTGKGMSEEDQKKLLNTETHFSKYGTNNEEGSGLGLLLCQDFAVKNGGRLW
CCEEEEEEECCCCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEEEEHHHCCCCCEEE
FESEEGKGSTFFFSVALKK
EECCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA