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The map label for this gene is wcaE [C]

Identifier: 150006448

GI number: 150006448

Start: 4885414

End: 4886229

Strand: Reverse

Name: wcaE [C]

Synonym: BVU_3967

Alternate gene names: 150006448

Gene position: 4886229-4885414 (Counterclockwise)

Preceding gene: 150006449

Following gene: 150006436

Centisome position: 94.64

GC content: 40.2

Gene sequence:

>816_bases
ATGTCACAAAGAAAATTTTCGATTATAACCATTAATTATAATAATAAAGAAGGGTTGAGGAAAACCATAGAAAGTGTGGT
CGGCCAGTCTTTTAGGGATTTTGAGTATATTATTATTGACGGAGGGTCTACGGATGGAAGTATTGAAGTGATAAAAGAGT
ATGCCGGTAAAGTGGACTATTGGGTATCGGAGCCGGACAAGGGTATTTATCATGCCATGAATAAAGGAGTTTTACAGGCG
CATGGAGAGTATTTGAACTTTATGAATTCCGGTGATGAATTTTATAACAATGGTGTGTTGCAGGAGGTGGCACCTTCATT
GGACAGCGATATTGTTGTTGGAAAAATAGTGCATGGCACAGAGGTGTGGGGATTTCATAAGGAGGATATAACGCTGATGG
ATTTGATTCGGGGAACTGTATTGCATCAGGCTTCATTCTTCCGTAAAGAACTTTTTGATGAAAACAGGTATGACGAGAGT
TATAAGATTGTATCCGATTGGAAATTTTATATTCAGACCTTGATATTCAACAATGCGACGTTCCGCAATATTCGTTCCAT
CGTTTGTAGGTTTGTACCCGGAGGGGTCAGTGAAACGGATGCCGGAACAAGAGATATGGAAAGAAAGCGGGTGTATAAGG
AGTTGTTTCCGGATAGAATGATGAAGGATTATATAAGACTGGAAAAGGTGGAATCTCCTTTATTGGAACTGATTCCCGAA
CTGAATAAAACTGCGGGCCTTCATCAGATGGCATACAAATTGGTCTGTGCCTTGTTGTGGGTGCATGGAAAGATAAAACG
GGTGCGTGTTAAGTAA

Upstream 100 bases:

>100_bases
CTTCGAATGTCAGCCGGATTGTGCCTCTGTATGTCGGATTCGAAAAATCGCTCAGTCTGGATGACGAATGGAAACTTTAG
ATTGTAAAAAGCGGAAAATT

Downstream 100 bases:

>100_bases
GCAGTAAATTGGTCTTTTCACTTGAGCCATGCCCGCAGGATAAGTCTGATGCCATATAGCGCATACTTTATTTTTAGAGA
AAGTGGAAGATGTATATAAT

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSQRKFSIITINYNNKEGLRKTIESVVGQSFRDFEYIIIDGGSTDGSIEVIKEYAGKVDYWVSEPDKGIYHAMNKGVLQA
HGEYLNFMNSGDEFYNNGVLQEVAPSLDSDIVVGKIVHGTEVWGFHKEDITLMDLIRGTVLHQASFFRKELFDENRYDES
YKIVSDWKFYIQTLIFNNATFRNIRSIVCRFVPGGVSETDAGTRDMERKRVYKELFPDRMMKDYIRLEKVESPLLELIPE
LNKTAGLHQMAYKLVCALLWVHGKIKRVRVK

Sequences:

>Translated_271_residues
MSQRKFSIITINYNNKEGLRKTIESVVGQSFRDFEYIIIDGGSTDGSIEVIKEYAGKVDYWVSEPDKGIYHAMNKGVLQA
HGEYLNFMNSGDEFYNNGVLQEVAPSLDSDIVVGKIVHGTEVWGFHKEDITLMDLIRGTVLHQASFFRKELFDENRYDES
YKIVSDWKFYIQTLIFNNATFRNIRSIVCRFVPGGVSETDAGTRDMERKRVYKELFPDRMMKDYIRLEKVESPLLELIPE
LNKTAGLHQMAYKLVCALLWVHGKIKRVRVK
>Mature_270_residues
SQRKFSIITINYNNKEGLRKTIESVVGQSFRDFEYIIIDGGSTDGSIEVIKEYAGKVDYWVSEPDKGIYHAMNKGVLQAH
GEYLNFMNSGDEFYNNGVLQEVAPSLDSDIVVGKIVHGTEVWGFHKEDITLMDLIRGTVLHQASFFRKELFDENRYDESY
KIVSDWKFYIQTLIFNNATFRNIRSIVCRFVPGGVSETDAGTRDMERKRVYKELFPDRMMKDYIRLEKVESPLLELIPEL
NKTAGLHQMAYKLVCALLWVHGKIKRVRVK

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788368, Length=221, Percent_Identity=31.6742081447964, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790044, Length=116, Percent_Identity=31.8965517241379, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 31372; Mature: 31241

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRKFSIITINYNNKEGLRKTIESVVGQSFRDFEYIIIDGGSTDGSIEVIKEYAGKVDY
CCCCEEEEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCE
WVSEPDKGIYHAMNKGVLQAHGEYLNFMNSGDEFYNNGVLQEVAPSLDSDIVVGKIVHGT
ECCCCCCHHHHHHHCHHHHHHHHHHHHHCCCHHHHHCCCHHHHHCCCCCCCEEEEEEECC
EVWGFHKEDITLMDLIRGTVLHQASFFRKELFDENRYDESYKIVSDWKFYIQTLIFNNAT
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCH
FRNIRSIVCRFVPGGVSETDAGTRDMERKRVYKELFPDRMMKDYIRLEKVESPLLELIPE
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
LNKTAGLHQMAYKLVCALLWVHGKIKRVRVK
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure 
SQRKFSIITINYNNKEGLRKTIESVVGQSFRDFEYIIIDGGSTDGSIEVIKEYAGKVDY
CCCEEEEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCE
WVSEPDKGIYHAMNKGVLQAHGEYLNFMNSGDEFYNNGVLQEVAPSLDSDIVVGKIVHGT
ECCCCCCHHHHHHHCHHHHHHHHHHHHHCCCHHHHHCCCHHHHHCCCCCCCEEEEEEECC
EVWGFHKEDITLMDLIRGTVLHQASFFRKELFDENRYDESYKIVSDWKFYIQTLIFNNAT
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCH
FRNIRSIVCRFVPGGVSETDAGTRDMERKRVYKELFPDRMMKDYIRLEKVESPLLELIPE
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
LNKTAGLHQMAYKLVCALLWVHGKIKRVRVK
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]